MD10G1321300.v1.1

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006279
Physical Location & Seq
Reverse (-)
40322994 .. 40327741
4748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1321300.v1.1.491

Sequence Viewer

Length: 3084 bp
ATGGAGTTCGAGAAACGAAAGGCGGCGACGCTAGCGTCATTGCGGTCGGAAGAGACAGACAAATCACCGAAGGGCACGGTGGACGCCCCAATCATACCCCTTCTCGACGGCATCAACAGCCACCCCAATTATTTCACCACCAGCTCCTGCTCCGGCCGTATCTCTATCCTCTCCCAGCCCACGCACGTGACAAATCAGCCCAAGACCAAGAAGAAAGCTCTAGGTGGCACGTGGATCTTCATCTCCCACGACCCGGCGGATGCCGACTCTATCCTCGGCCTCCTCTTCGGCCCCGACCCGAATGATCAGAACGACGCACAGGACCAAAACGACCTCGTTTTCAGATTCGAGCCGCTTATTGTGGCGGTGGAGTGCAGGGACGTGGCGTCGGCGCAGTCGTTGGTTTCGAAGGCGATTGCTTGCGGGTTCAGAGAGTCCGGCATTACGAATTCGAACCGGCGCGTGATTATCGCCATTCGGTGTTCAATTCGGATGGAGGTGCCGCTGGGGAGCAGCCGGGAGGTGATGGTGTCGAGAGAGTACGTGAGGTTCCTTGTCAGGGTGGCGAACGAGAAAATGGAGGCCAACAGGAAGAGGACTGAGGCATTTTTCCAGGCTCTGCAGAGTGAAGGTGGAGGGTTTGTGGGTCCCACGGTTGCGAATGGCGAGATTGCGGCAGATGGGCATTCGGATTCTGGGTCCAAGGAAGTTCCTGGTGGTTGTAGTCTGTCTGTTGTTGAGATGGAAATAGCCGGTGAACCCGAAGAGAATCTTTTCCTCTGGGGTCATTCGTCTTGTGCATTGGAAATCAAAAACCGGAATGGTGTTCTTGTGTTTGGTGGCTTTGGAGGCATGGGAAGACATGGGAGAAGAAGTAGTTCTTGGCTGGTTGATCCATTGTCTGGCACTTTGAGAGCAATTGATGCAGACGGCACTCCGTCTCCGCGGTTGGGTCACACTACGTCTCTGGTTGGAGATTGTGTGTTTGTCATTGGAGGCAGGGCTGATCCTGAGAAAATTTTGAGTGATGTTTGGGTCCTCAATACAGCAAAGAATGAATGGAAGTTATTGGAATGTTCTGGGGACAAATTTCCTCCCAGGCATCGGCATGCTGCAGCTGTTATAGGCTCAAAGATATATGTGTTTGGTGGACTTAACAATGATACCATCAGTTCTTCCTTACATGTTCTCGATACCGATAATCTGCAGTGGAAAGAGTTGATTGTCAGTGGTGAGCATCCATGTGCCAGACATTCTCACTCAATGGTGGCATATAGATCTCAACTATATATTTTTGGAGGATATAGTGGTGAGAAAACACTTGCAGACTTGTATGTTTTTGATATCCAAACATGTAAATGGAAGAATGAAAATGCAGCTGGGAGAAGTCCACATGCAAGGTTCTCCCACTCCATGTTTGTCTACAAGAATTATCTTGGGATTGTTGGTGGTTGTCCAGTTAAACAACGTTGCCAGGAGTTAGCACTACTTGATTTGAAGGTCCGTGTGTGGAGGCATGCGAAACTTGAATCCAATGGTGAAGATTTATTTGTACGTAGCACAGCCAATGTTGTCGGCGACAATCTTATTATGATTGGTGGTGGGGCATCTTGTTATGCATTTGGGACAAAGTTTAGTAAGCCCATGAAAATCAACTTGCTTCCTCTTATGAGCATAGATGATAATATTAAGCCAGATGACAGAGAAATGCATGGTCAACATTATGAAATGGTGAATAATGAGAAAGGTAAACAATTCCAAGATCCACAGGCTGAAGGTGTGCAGAGCTTAAGTGAACCTTGTGATCTGAATTTTGAGAGTGACTTGCCCATGGAAAATGGCATAAGTCAGCAGGTAGAGTCATATTGGATTCTACAACTTGAAAGAAAATATGCAAAAGTAGGCAAGGACATATTGAAGAAGTTTGGATGGTTAGATCTTGGAAGGAAGGTGTACTCCAGGAAGAGTGGTTCACATATATGTTTTCCTGTGAACGAAAAATTTTCAGGTGTGTTTAAAGAAAACAAGCGTCCTTTGACAGATTTATCTGAAGGACAGAGTGATCACTTTGTTAAACCAGTTATAGGAGAAGAATTCTCGGTTAATGAGGTGAACTGTTCAAAAGCCTTGGACATTCTAAAGGAATGTGGTGCTACTAAGCTGGCAGCTGAAGAAGTTGAAGTCAAGAGAGCTGCCAAATCTCCCGCGAAAGTAATGAATGAAATTGTGGGCTTGCTGATAAAGGAGAAAGGACTGCCTGCAGAACTCTTAGAGGAGCTACCAGCAAGATGGGAGCGACTTGGAGATATAGTTGTCCTTCCGGCGACATCGTTCAAGAATCCAATATGGGACTCATTAGGGATGGAGCTTTGGCATGTTATTTCCAAATCACTTAACGCTCATCGCCTTGCACGCCAAGGCCGAGTTGCGTCGACCGCTACAAGGGACAGTACTTTGGAGATTCTTGTAGGAGATAATGGATGGGTTGAGCATCGCGAAAATGGCATTCTCTATTCTTTCGATGCTACTAAATGTATGTTCTCCTGGGGCAACCTATCTGAGAAGCTCCGCATGGCGTCAATGAATTGCAGAAATGAGATTGTTGTGGATTTGTTTGCTGGAATTGGATATTTTGTTCTGCCATTTCTTGTCAGGGCCAACGCAAAACTAGTTTATGCATGTGAATGGAATCCCCATGCTGTTGAGGCACTCCGGCGAAATGTGCAAGCCAATTCAGTTAGTGACCGATGCATTGTTCTTGAAGGGGATAACCGGATAGTGGCACCTAAAGGAGTGGCTGATCGGGTCTGTCTCGGTCTCATTCCAACAAGTGAAGGTAGCTGGGTCACTGCTGTTAGGGCATTAAGGAGCGAGGGCGGGATGCTACACGTGCACGGGAACGTCAAGGACTCGGAGGAGAATTCATGGACTAAACATGTATCAGAATCAATAAGTGAAATTGCCAAATCTGAAGGTCTTAGTTGGGAAGTAAGAGTAGAACATGTCGAGAGAGTGAAATGGTATGGCCCACACATCCGTCATCTTGTTGCAGACGTTAGATGTCGACAGATGATCCGAACATGA

Protein Analysis

1028

Amino Acids

113.73

Weight (kDa)

6.69

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 208 2.3e-69 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 263 - 384 1.1e-22 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 273 - 376 1.5e-14 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 310 - 358 5.6e-06 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 313 - 546 2.7e-26 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 315 - 357 5.1e-08 Kelch motif
Kelch_2 PF07646 315 - 359 1.2e-06 Kelch motif
NANM PF24996 321 - 450 4.2e-06 N-acetylneuraminate epimerase
Beta-prop_Calicin PF13964 326 - 456 1.6e-08 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 342 - 413 3.4e-11 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 366 - 407 9.9e-10 Kelch motif
Kelch_2 PF07646 366 - 409 9.9e-09 Kelch motif
Beta-prop_FBX42 PF13415 367 - 536 1.7e-30 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 370 - 539 5.5e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 401 - 485 1.5e-11 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 403 - 461 1.4e-11 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 412 - 534 5.3e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 416 - 455 3.2e-08 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 429 - 541 2.8e-14 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 749 - 826 4.9e-15 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 830 - 989 3.2e-35 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
Acc36I ACCTGC 1 cut(s) 1843
AccB1I GGYRCC 2 cut(s) 499, 2782
AccB7I CCANNNNNTGG 1 cut(s) 902
AccI GTMKAC 3 cut(s) 1422, 2432, 3064
AccII CGCG 4 cut(s) 462, 946, 2207, 2496
AclI AACGTT 1 cut(s) 1468
AclWI GGATC 5 cut(s) 242, 887, 1001, 1757, 3067
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 7 cut(s) 448, 1017, 1088, 1810, 2000, 2093, 2920
AcuI CTGAAG 4 cut(s) 1794, 2070, 2190, 2991
AcvI CACGTG 3 cut(s) 187, 231, 2890
AcyI GRCGYC 3 cut(s) 84, 386, 2576
AfaI GTAC 4 cut(s) 542, 1554, 1955, 2452
AfiI CCNNNNNNNGG 8 cut(s) 253, 559, 902, 950, 1104, 2084, 2442, 2779
AflII CTTAAG 1 cut(s) 1789
AflIII ACRYGT 5 cut(s) 1183, 1352, 2887, 2935, 3001
AgsI TTSAA 9 cut(s) 486, 1498, 1529, 1883, 1918, 2121, 2180, 2335, 2762
AhdI GACNNNNNGTC 1 cut(s) 385
AhlI ACTAGT 1 cut(s) 2668
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 6 cut(s) 612, 712, 1097, 1473, 1958, 2543
AjuI GAANNNNNNNTTGG 2 cut(s) 865, 897
AleI CACNNNNGTG 1 cut(s) 185
AloI GAACNNNNNNTCC 4 cut(s) 1954, 1986, 2619, 2651
Alw21I GWGCWC 1 cut(s) 2895
Alw26I GTCTC 5 cut(s) 47, 945, 969, 2816, 2822
Alw44I GTGCAC 1 cut(s) 2891
AlwI GGATC 5 cut(s) 242, 887, 1001, 1757, 3067
AlwNI CAGNNNCTG 2 cut(s) 147, 619
AoxI GGCC 7 cut(s) 154, 277, 289, 582, 2419, 2655, 3025
ApaLI GTGCAC 1 cut(s) 2891
ApeKI GCWGC 6 cut(s) 513, 1112, 1115, 1376, 2165, 2192
ApoI RAATTY 7 cut(s) 448, 1017, 1088, 1810, 2000, 2093, 2920
Asp700I GAANNNNTTC 2 cut(s) 773, 877
AspLEI GCGC 2 cut(s) 394, 462
AspS9I GGNCC 8 cut(s) 290, 322, 647, 699, 1036, 1501, 2655, 3026
AsuC2I CCSGG 2 cut(s) 254, 518
AsuHPI GGTGA 9 cut(s) 57, 127, 535, 767, 1244, 1322, 1550, 1744, 2122
AsuII TTCGAA 2 cut(s) 407, 452
AsuNHI GCTAGC 1 cut(s) 31
AvaII GGWCC 5 cut(s) 322, 647, 699, 1036, 1501
BaeGI GKGCMC 2 cut(s) 77, 2895
BanI GGYRCC 2 cut(s) 499, 2782
BbrPI CACGTG 3 cut(s) 187, 231, 2890
BbsI GAAGAC 1 cut(s) 865
Bbv12I GWGCWC 1 cut(s) 2895
BbvI GCAGC 6 cut(s) 525, 1099, 1127, 1388, 2177, 2179
BccI CCATC 9 cut(s) 487, 520, 674, 736, 1175, 1923, 2283, 2356, 2475
BceAI ACGGC 3 cut(s) 124, 141, 946
BciT130I CCWGG 6 cut(s) 614, 714, 1099, 1475, 1960, 2545
BclI TGATCA 2 cut(s) 304, 2062
BcnI CCSGG 2 cut(s) 254, 518
BcoDI GTCTC 5 cut(s) 47, 945, 969, 2816, 2822
BcuI ACTAGT 1 cut(s) 2668
BfaI CTAG 3 cut(s) 32, 221, 2669
BfmI CTRYAG 4 cut(s) 620, 1113, 1205, 2259
BfrI CTTAAG 1 cut(s) 1789
BfuAI ACCTGC 1 cut(s) 1843
BglII AGATCT 2 cut(s) 1277, 1936
BmcAI AGTACT 1 cut(s) 2452
Bme1390I CCNGG 8 cut(s) 254, 518, 614, 714, 1099, 1475, 1960, 2545
Bme18I GGWCC 5 cut(s) 322, 647, 699, 1036, 1501
BmeRI GACNNNNNGTC 1 cut(s) 385
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 8 cut(s) 290, 322, 647, 699, 1036, 1501, 2655, 3026
BmiI GGNNCC 8 cut(s) 292, 501, 551, 648, 649, 700, 1037, 2784
BmrFI CCNGG 8 cut(s) 254, 518, 614, 714, 1099, 1475, 1960, 2545
BmtI GCTAGC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 865
BpmI CTGGAG 1 cut(s) 1942
Bpu14I TTCGAA 2 cut(s) 407, 452
BpuMI CCSGG 2 cut(s) 254, 518
BsaAI YACGTR 5 cut(s) 187, 231, 544, 1556, 2890
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 3 cut(s) 84, 386, 2576
BsaI GGTCTC 1 cut(s) 2822
BsaJI CCNNGG 9 cut(s) 274, 651, 702, 944, 1097, 1830, 2127, 2416, 2544
BsaWI WCCGGW 2 cut(s) 816, 2772
Bsc4I CCNNNNNNNGG 8 cut(s) 253, 559, 902, 950, 1104, 2084, 2442, 2779
Bse118I RCCGGY 2 cut(s) 456, 752
Bse1I ACTGG 2 cut(s) 1457, 2078
Bse3DI GCAATG 1 cut(s) 38
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 6 cut(s) 614, 714, 1099, 1475, 1960, 2545
BseDI CCNNGG 9 cut(s) 274, 651, 702, 944, 1097, 1830, 2127, 2416, 2544
BseGI GGATG 8 cut(s) 265, 498, 1237, 1934, 2367, 2486, 2886, 3033
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 8 cut(s) 253, 559, 902, 950, 1104, 2084, 2442, 2779
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 591, 1002, 2550
BseNI ACTGG 2 cut(s) 1457, 2078
BseRI GAGGAG 3 cut(s) 272, 2288, 2930
BseSI GKGCMC 2 cut(s) 77, 2895
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 6 cut(s) 525, 1099, 1127, 1388, 2177, 2179
BseYI CCCAGC 4 cut(s) 174, 505, 1379, 2841
BsgI GTGCAG 2 cut(s) 394, 1802
Bsh1236I CGCG 4 cut(s) 462, 946, 2207, 2496
Bsh1285I CGRYCG 3 cut(s) 47, 157, 2436
BshFI GGCC 7 cut(s) 156, 279, 291, 584, 2421, 2657, 3027
BshNI GGYRCC 2 cut(s) 499, 2782
BsiEI CGRYCG 3 cut(s) 47, 157, 2436
BsiHKAI GWGCWC 1 cut(s) 2895
BslFI GGGAC 6 cut(s) 392, 633, 1097, 1639, 2363, 2459
BslI CCNNNNNNNGG 8 cut(s) 253, 559, 902, 950, 1104, 2084, 2442, 2779
BsmAI GTCTC 5 cut(s) 47, 945, 969, 2816, 2822
BsmBI CGTCTC 2 cut(s) 945, 969
BsmFI GGGAC 6 cut(s) 392, 633, 1097, 1639, 2363, 2459
BsmI GAATGC 2 cut(s) 685, 2505
BsnI GGCC 7 cut(s) 156, 279, 291, 584, 2421, 2657, 3027
Bso31I GGTCTC 1 cut(s) 2822
Bsp119I TTCGAA 2 cut(s) 407, 452
Bsp1286I GDGCHC 2 cut(s) 77, 2895
Bsp19I CCATGG 1 cut(s) 1830
Bsp68I TCGCGA 1 cut(s) 2496
BspANI GGCC 7 cut(s) 156, 279, 291, 584, 2421, 2657, 3027
BspCNI CTCAG 3 cut(s) 592, 1003, 2551
BspFNI CGCG 4 cut(s) 462, 946, 2207, 2496
BspLI GGNNCC 8 cut(s) 292, 501, 551, 648, 649, 700, 1037, 2784
BspMAI CTGCAG 4 cut(s) 624, 1117, 1209, 2263
BspMI ACCTGC 1 cut(s) 1843
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 5 cut(s) 242, 887, 1001, 1757, 3067
BspT104I TTCGAA 2 cut(s) 407, 452
BspT107I GGYRCC 2 cut(s) 499, 2782
BspTI CTTAAG 1 cut(s) 1789
BspTNI GGTCTC 1 cut(s) 2822
BsrDI GCAATG 1 cut(s) 38
BsrFI RCCGGY 2 cut(s) 456, 752
BsrI ACTGG 2 cut(s) 1457, 2078
BssAI RCCGGY 2 cut(s) 456, 752
BssECI CCNNGG 9 cut(s) 274, 651, 702, 944, 1097, 1830, 2127, 2416, 2544
BssNI GRCGYC 3 cut(s) 84, 386, 2576
BssT1I CCWWGG 4 cut(s) 702, 1830, 2127, 2416
Bst2UI CCWGG 6 cut(s) 614, 714, 1099, 1475, 1960, 2545
Bst4CI ACNGT 4 cut(s) 79, 655, 2117, 2450
Bst6I CTCTTC 5 cut(s) 45, 290, 587, 759, 1958
BstACI GRCGYC 3 cut(s) 84, 386, 2576
BstAFI CTTAAG 1 cut(s) 1789
BstAPI GCANNNNNTGC 1 cut(s) 923
BstBAI YACGTR 5 cut(s) 187, 231, 544, 1556, 2890
BstBI TTCGAA 2 cut(s) 407, 452
BstC8I GCNNGC 9 cut(s) 33, 421, 1110, 1518, 2163, 2234, 2259, 2413, 2727
BstDEI CTNAG 6 cut(s) 600, 1011, 2157, 2269, 2559, 2978
BstDSI CCRYGG 3 cut(s) 651, 944, 1830
BstF5I GGATG 8 cut(s) 265, 498, 1237, 1934, 2367, 2486, 2886, 3033
BstFNI CGCG 4 cut(s) 462, 946, 2207, 2496
BstHHI GCGC 2 cut(s) 394, 462
BstMAI GTCTC 5 cut(s) 47, 945, 969, 2816, 2822
BstMCI CGRYCG 3 cut(s) 47, 157, 2436
BstNI CCWGG 6 cut(s) 614, 714, 1099, 1475, 1960, 2545
BstNSI RCATGY 9 cut(s) 1112, 1187, 1356, 1397, 1520, 2378, 2682, 2939, 3005
BstSCI CCNGG 8 cut(s) 252, 516, 612, 712, 1097, 1473, 1958, 2543
BstSFI CTRYAG 4 cut(s) 620, 1113, 1205, 2259
BstSLI GKGCMC 2 cut(s) 77, 2895
BstSNI TACGTA 1 cut(s) 1556
BstUI CGCG 4 cut(s) 462, 946, 2207, 2496
BstV1I GCAGC 6 cut(s) 525, 1099, 1127, 1388, 2177, 2179
BstV2I GAAGAC 1 cut(s) 865
BstX2I RGATCY 4 cut(s) 234, 1277, 1762, 1936
BstXI CCANNNNNNTGG 1 cut(s) 2289
BstYI RGATCY 4 cut(s) 234, 1277, 1762, 1936
BstZI CGGCCG 1 cut(s) 154
BsuRI GGCC 7 cut(s) 156, 279, 291, 584, 2421, 2657, 3027
BtgI CCRYGG 3 cut(s) 651, 944, 1830
BtgZI GCGATG 2 cut(s) 2387, 2477
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 8 cut(s) 265, 498, 1237, 1934, 2367, 2486, 2886, 3033
BtsI GCAGTG 2 cut(s) 1214, 2847
BtsIMutI CAGTG 3 cut(s) 1214, 1234, 2847
BtuMI TCGCGA 1 cut(s) 2496
BveI ACCTGC 1 cut(s) 1843
Cac8I GCNNGC 9 cut(s) 33, 421, 1110, 1518, 2163, 2234, 2259, 2413, 2727
CaiI CAGNNNCTG 2 cut(s) 147, 619
CfoI GCGC 2 cut(s) 394, 462
Cfr10I RCCGGY 2 cut(s) 456, 752
Cfr13I GGNCC 8 cut(s) 290, 322, 647, 699, 1036, 1501, 2655, 3026
Cfr42I CCGCGG 1 cut(s) 947
CseI GACGC 8 cut(s) 24, 37, 92, 323, 375, 2018, 2418, 2565
Csp6I GTAC 4 cut(s) 541, 1553, 1954, 2451
CviQI GTAC 4 cut(s) 541, 1553, 1954, 2451
DdeI CTNAG 6 cut(s) 600, 1011, 2157, 2269, 2559, 2978
DraI TTTAAA 1 cut(s) 2017
DrdI GACNNNNNNGTC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 154
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 5 cut(s) 45, 290, 587, 759, 1958
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 5 cut(s) 45, 290, 587, 759, 1958
EciI GGCGGA 1 cut(s) 272
EclXI CGGCCG 1 cut(s) 154
Eco105I TACGTA 1 cut(s) 1556
Eco130I CCWWGG 4 cut(s) 702, 1830, 2127, 2416
Eco31I GGTCTC 1 cut(s) 2822
Eco32I GATATC 1 cut(s) 1345
Eco47I GGWCC 5 cut(s) 322, 647, 699, 1036, 1501
Eco52I CGGCCG 1 cut(s) 154
Eco57I CTGAAG 4 cut(s) 1794, 2070, 2190, 2991
Eco72I CACGTG 3 cut(s) 187, 231, 2890
EcoO109I RGGNCCY 2 cut(s) 647, 1036
EcoRI GAATTC 3 cut(s) 448, 2093, 2920
EcoRII CCWGG 6 cut(s) 612, 712, 1097, 1473, 1958, 2543
EcoRV GATATC 1 cut(s) 1345
EcoT14I CCWWGG 4 cut(s) 702, 1830, 2127, 2416
EcoT22I ATGCAT 4 cut(s) 1621, 1713, 2680, 2753
ErhI CCWWGG 4 cut(s) 702, 1830, 2127, 2416
Esp3I CGTCTC 2 cut(s) 945, 969
FalI AAGNNNNNCTT 6 cut(s) 756, 788, 865, 897, 1783, 1815
FaqI GGGAC 6 cut(s) 392, 633, 1097, 1639, 2363, 2459
FauI CCCGC 3 cut(s) 416, 2212, 2870
FbaI TGATCA 2 cut(s) 304, 2062
FblI GTMKAC 3 cut(s) 1422, 2432, 3064
FokI GGATG 8 cut(s) 272, 505, 1224, 1941, 2374, 2493, 2893, 3020
FspBI CTAG 3 cut(s) 32, 221, 2669
GlaI GCGC 2 cut(s) 393, 461
GsaI CCCAGC 4 cut(s) 178, 509, 1383, 2845
GsuI CTGGAG 1 cut(s) 1942
HaeIII GGCC 7 cut(s) 156, 279, 291, 584, 2421, 2657, 3027
HgaI GACGC 8 cut(s) 24, 37, 92, 323, 375, 2018, 2418, 2565
HhaI GCGC 2 cut(s) 394, 462
Hin1I GRCGYC 3 cut(s) 84, 386, 2576
Hin6I GCGC 2 cut(s) 392, 460
HinP1I GCGC 2 cut(s) 392, 460
HincII GTYRAC 3 cut(s) 1718, 2433, 3065
HindII GTYRAC 3 cut(s) 1718, 2433, 3065
HphI GGTGA 9 cut(s) 57, 127, 535, 767, 1244, 1322, 1550, 1744, 2122
Hpy99I CGWCG 5 cut(s) 31, 110, 317, 391, 2434
HpyCH4III ACNGT 4 cut(s) 79, 655, 2117, 2450
HpyF3I CTNAG 6 cut(s) 600, 1011, 2157, 2269, 2559, 2978
Hsp92I GRCGYC 3 cut(s) 84, 386, 2576
HspAI GCGC 2 cut(s) 392, 460
KflI GGGWCCC 1 cut(s) 647
Ksp22I TGATCA 2 cut(s) 304, 2062
KspI CCGCGG 1 cut(s) 947
LmnI GCTCC 8 cut(s) 149, 155, 510, 2275, 2293, 2365, 2571, 2868
Lsp1109I GCAGC 6 cut(s) 525, 1099, 1127, 1388, 2177, 2179
MaeI CTAG 3 cut(s) 32, 221, 2669
MaeIII GTNAC 5 cut(s) 187, 953, 1820, 2741, 2845
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 4 cut(s) 234, 1277, 1762, 1936
MhlI GDGCHC 2 cut(s) 77, 2895
MlyI GAGTC 5 cut(s) 260, 443, 1868, 2345, 2903
MmeI TCCRAC 3 cut(s) 27, 952, 2849
Mph1103I ATGCAT 4 cut(s) 1621, 1713, 2680, 2753
MroXI GAANNNNTTC 2 cut(s) 773, 877
MseI TTAA 9 cut(s) 1155, 1461, 1689, 1790, 2016, 2073, 2103, 2394, 2864
MslI CAYNNNNRTG 6 cut(s) 185, 1107, 1242, 1357, 1978, 2683
MspA1I CMGCKG 5 cut(s) 505, 946, 1118, 1379, 2168
MspCI CTTAAG 1 cut(s) 1789
MspR9I CCNGG 8 cut(s) 254, 518, 614, 714, 1099, 1475, 1960, 2545
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 2 cut(s) 685, 2505
MvaI CCWGG 6 cut(s) 614, 714, 1099, 1475, 1960, 2545
MvnI CGCG 4 cut(s) 462, 946, 2207, 2496
NciI CCSGG 2 cut(s) 254, 518
NcoI CCATGG 1 cut(s) 1830
NheI GCTAGC 1 cut(s) 31
NlaIV GGNNCC 8 cut(s) 292, 501, 551, 648, 649, 700, 1037, 2784
NmeAIII GCCGAG 2 cut(s) 255, 2447
NmuCI GTSAC 5 cut(s) 187, 953, 1820, 2741, 2845
NruI TCGCGA 1 cut(s) 2496
NsiI ATGCAT 4 cut(s) 1621, 1713, 2680, 2753
NspI RCATGY 9 cut(s) 1112, 1187, 1356, 1397, 1520, 2378, 2682, 2939, 3005
NspV TTCGAA 2 cut(s) 407, 452
OliI CACNNNNGTG 1 cut(s) 185
PaeI GCATGC 2 cut(s) 1112, 1520
PciI ACATGT 4 cut(s) 1183, 1352, 2935, 3001
PcsI WCGNNNNNNNCGW 3 cut(s) 395, 404, 2419
PctI GAATGC 2 cut(s) 685, 2505
PdmI GAANNNNTTC 2 cut(s) 773, 877
PfeI GAWTC 9 cut(s) 345, 692, 769, 1529, 1870, 2338, 2461, 2689, 2945
PflMI CCANNNNNTGG 1 cut(s) 902
PfoI TCCNGGA 1 cut(s) 1958
PleI GAGTC 5 cut(s) 260, 442, 1867, 2345, 2903
PmaCI CACGTG 3 cut(s) 187, 231, 2890
PmlI CACGTG 3 cut(s) 187, 231, 2890
PpsI GAGTC 5 cut(s) 260, 442, 1867, 2345, 2903
Ppu21I YACGTR 5 cut(s) 187, 231, 544, 1556, 2890
PpuMI RGGWCCY 2 cut(s) 647, 1036
PscI ACATGT 4 cut(s) 1183, 1352, 2935, 3001
Psp1406I AACGTT 1 cut(s) 1468
Psp5II RGGWCCY 2 cut(s) 647, 1036
Psp6I CCWGG 6 cut(s) 612, 712, 1097, 1473, 1958, 2543
PspCI CACGTG 3 cut(s) 187, 231, 2890
PspFI CCCAGC 4 cut(s) 174, 505, 1379, 2841
PspGI CCWGG 6 cut(s) 612, 712, 1097, 1473, 1958, 2543
PspN4I GGNNCC 8 cut(s) 292, 501, 551, 648, 649, 700, 1037, 2784
PspPI GGNCC 8 cut(s) 290, 322, 647, 699, 1036, 1501, 2655, 3026
PspPPI RGGWCCY 2 cut(s) 647, 1036
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
PstI CTGCAG 4 cut(s) 624, 1117, 1209, 2263
PstNI CAGNNNCTG 2 cut(s) 147, 619
PsuI RGATCY 4 cut(s) 234, 1277, 1762, 1936
PvuII CAGCTG 3 cut(s) 1118, 1379, 2168
RruI TCGCGA 1 cut(s) 2496
RsaI GTAC 4 cut(s) 542, 1554, 1955, 2452
RsaNI GTAC 4 cut(s) 541, 1553, 1954, 2451
RseI CAYNNNNRTG 6 cut(s) 185, 1107, 1242, 1357, 1978, 2683
SacII CCGCGG 1 cut(s) 947
SalI GTCGAC 2 cut(s) 2431, 3063
SaqAI TTAA 9 cut(s) 1155, 1461, 1689, 1790, 2016, 2073, 2103, 2394, 2864
Sau96I GGNCC 8 cut(s) 290, 322, 647, 699, 1036, 1501, 2655, 3026
ScaI AGTACT 1 cut(s) 2452
SchI GAGTC 5 cut(s) 260, 443, 1868, 2345, 2903
ScrFI CCNGG 8 cut(s) 254, 518, 614, 714, 1099, 1475, 1960, 2545
SduI GDGCHC 2 cut(s) 77, 2895
SfcI CTRYAG 4 cut(s) 620, 1113, 1205, 2259
Sfr303I CCGCGG 1 cut(s) 947
SfuI TTCGAA 2 cut(s) 407, 452
SgrBI CCGCGG 1 cut(s) 947
SinI GGWCC 5 cut(s) 322, 647, 699, 1036, 1501
SmiMI CAYNNNNRTG 6 cut(s) 185, 1107, 1242, 1357, 1978, 2683
SmlI CTYRAG 1 cut(s) 1789
SmoI CTYRAG 1 cut(s) 1789
SnaBI TACGTA 1 cut(s) 1556
SpeI ACTAGT 1 cut(s) 2668
SphI GCATGC 2 cut(s) 1112, 1520
SspI AATATT 1 cut(s) 1687
SspMI CTAG 3 cut(s) 32, 221, 2669
StyD4I CCNGG 8 cut(s) 252, 516, 612, 712, 1097, 1473, 1958, 2543
StyI CCWWGG 4 cut(s) 702, 1830, 2127, 2416
TaaI ACNGT 4 cut(s) 79, 655, 2117, 2450
TaqII GACCGA 2 cut(s) 2760, 2804
TatI WGTACW 2 cut(s) 1953, 2450
TauI GCSGC 4 cut(s) 26, 355, 505, 677
TfiI GAWTC 9 cut(s) 345, 692, 769, 1529, 1870, 2338, 2461, 2689, 2945
Tru1I TTAA 9 cut(s) 1155, 1461, 1689, 1790, 2016, 2073, 2103, 2394, 2864
Tru9I TTAA 9 cut(s) 1155, 1461, 1689, 1790, 2016, 2073, 2103, 2394, 2864
TscAI CASTG 3 cut(s) 1214, 1234, 2854
TseFI GTSAC 5 cut(s) 187, 953, 1820, 2741, 2845
TseI GCWGC 6 cut(s) 513, 1112, 1115, 1376, 2165, 2192
Tsp45I GTSAC 5 cut(s) 187, 953, 1820, 2741, 2845
TspDTI ATGAA 9 cut(s) 229, 1071, 1383, 1661, 1740, 2231, 2235, 2597, 2913
TspGWI ACGGA 3 cut(s) 927, 1493, 3026
TspRI CASTG 3 cut(s) 1214, 1234, 2854
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 1 cut(s) 1789
VneI GTGCAC 1 cut(s) 2891
VpaK11BI GGWCC 5 cut(s) 322, 647, 699, 1036, 1501
XapI RAATTY 7 cut(s) 448, 1017, 1088, 1810, 2000, 2093, 2920
XceI RCATGY 9 cut(s) 1112, 1187, 1356, 1397, 1520, 2378, 2682, 2939, 3005
XmiI GTMKAC 3 cut(s) 1422, 2432, 3064
XmnI GAANNNNTTC 2 cut(s) 773, 877
XspI CTAG 3 cut(s) 32, 221, 2669
ZrmI AGTACT 1 cut(s) 2452
Zsp2I ATGCAT 4 cut(s) 1621, 1713, 2680, 2753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.