AT4G09740

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010905
Physical Location & Seq
Reverse (-)
6142706 .. 6145003
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09740.1

Sequence Viewer

Length: 1437 bp
ATGGGCAAACTCTTGGTTGTAATGTTAATTGGGATGTTCTTGGCTTTCGAAAGCTTGGAAGCTCTTGACTATGGAGATGCACTGAACAAATCAATTTTGTTCTTTGAAGGCCAAAGATCTGGTAAACTCCCGACTAATCAAAGGGTTAAATGGCGAGCTGATTCTGGCCTGTCTGACGGTGCATCAGCTAATGTGAATTTGATTGGAGGATATTACGATGCTGGTGACAATGTAAAATTTGTGTGGCCAATGTCATTTACCACAACCTTGTTGAGTTGGGCTGCACTTGAATACCAGAATGAGATTACTTTCGTTAACCAGCTTGGTTATCTCCGGTCTACTATCAAATGGGGAACTAATTTTATCCTCCGTGCTCATACGTCGACCAACATGCTATATACACAGGTAGGAGATGGAAATTCAGATCATAGTTGTTGGGAGAGACCAGAAGATATGGACACACCTAGAACTCTCTATAGTATCTCTTCCTCTTCCCCCGGTTCCGAAGCCGCAGGTGAAGCCGCAGCCGCTCTTGCCGCGGCCTCACTTGTTTTCAAACTGGTTGATTCTACATATTCATCCAAATTACTAAACAATGCTAAATCCCTATTTGAATTTGCTGATAAGTACAGAGGTTCTTACCAAGCTTCTTGCCCCTTCTACTGCTCACACTCAGGCTACCAGGATGAACTTTTGTGGGCTGCGGCTTGGTTATACAAAGCAACAGGGGAAAAGAGTTACTTAAATTATGTTATAAGCAACAAAGATTGGAGCAAAGCCATCAATGAATTTAGTTGGGACAACAAATTCGCCGGCGTTCAAGCTTTACTCGCATCGGAGTTTTACAACGGGGCAAATGACTTGGAAAAATTTAAGACCGATGTTGAATCATTCGTCTGTGCATTGATGCCAGGAAGTAGCTCTCAACAAATTAAGCCAACTCCTGGTGGCATTTTGTTTATTAGAGACAGTAGCAACTTACAGTATGTAACAACGGCTACAACTATATTATTCTATTACTCAAAAACTCTAACCAAAGCCGGGGTTGGTTCAATCCAGTGTGGGTCGACTCAGTTCACCGTATCTCAAATTCGAAATTTTGCCAAGTCACAGGTTGATTACATTCTTGGAAATAATCCATTGAAAATGTCTTACATGGTTGGATTTGGAACAAAGTATCCTACACAACCTCATCATAGAGGCTCGTCATTACCGTCAATCCAATCTAAACCAGAGAAAATCGACTGTAATGGAGGATTTTCATACTACAATTTTGATACACCAAACCCGAATGTGCATACCGGCGCAATTGTGGGCGGACCAAATTCATCCGACCAGTATAGCGACAAAAGAACAGATTATTCTCATGCAGAGCCCACAACTTACATCAATGCCGCCTTTATTGGGTCAGTCGCTGCTCTGATCAGTTCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

478

Amino Acids

52.51

Weight (kDa)

6.01

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 24 - 469 2.6e-161 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AarI CACCTGC 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 944
AccBSI CCGCTC 1 cut(s) 530
AccI GTMKAC 3 cut(s) 338, 383, 1067
AccII CGCG 1 cut(s) 539
AciI CCGC 8 cut(s) 510, 522, 528, 537, 539, 704, 1317, 1395
AcoI YGGCCR 1 cut(s) 245
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 944, 1041, 1404
AgsI TTSAA 8 cut(s) 107, 290, 556, 614, 821, 887, 1053, 1144
AjnI CCWGG 3 cut(s) 681, 910, 943
AloI GAACNNNNNNTCC 2 cut(s) 1162, 1194
AluBI AGCT 8 cut(s) 54, 62, 158, 188, 322, 647, 824, 921
AluI AGCT 8 cut(s) 54, 62, 158, 188, 322, 647, 824, 921
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 2 cut(s) 436, 960
AlwNI CAGNNNCTG 1 cut(s) 1415
AoxI GGCC 4 cut(s) 109, 166, 245, 540
ApeKI GCWGC 4 cut(s) 281, 524, 701, 1415
ArsI GACNNNNNNTTYG 2 cut(s) 791, 823
AspLEI GCGC 1 cut(s) 1307
AspS9I GGNCC 1 cut(s) 1319
AsuC2I CCSGG 2 cut(s) 498, 1042
AsuHPI GGTGA 3 cut(s) 236, 527, 1069
AsuII TTCGAA 2 cut(s) 48, 1093
AvaII GGWCC 1 cut(s) 1319
BalI TGGCCA 1 cut(s) 247
BanII GRGCYC 1 cut(s) 1377
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 4 cut(s) 268, 536, 688, 1402
BccI CCATC 2 cut(s) 407, 788
BceAI ACGGC 1 cut(s) 1011
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 3 cut(s) 683, 912, 945
BciVI GTATCC 1 cut(s) 1188
BclI TGATCA 1 cut(s) 1422
BcnI CCSGG 2 cut(s) 498, 1042
BcoDI GTCTC 2 cut(s) 436, 960
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 475
BfuAI ACCTGC 1 cut(s) 503
BfuI GTATCC 1 cut(s) 1188
BglII AGATCT 1 cut(s) 116
Bme1390I CCNGG 5 cut(s) 498, 683, 912, 945, 1042
Bme18I GGWCC 1 cut(s) 1319
BmgT120I GGNCC 1 cut(s) 1319
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 5 cut(s) 498, 683, 912, 945, 1042
BmsI GCATC 5 cut(s) 67, 191, 208, 842, 897
Bpu14I TTCGAA 2 cut(s) 48, 1093
BpuMI CCSGG 2 cut(s) 498, 1042
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 496, 537, 1041
BsaWI WCCGGW 1 cut(s) 333
Bsc4I CCNNNNNNNGG 3 cut(s) 944, 1041, 1404
Bse118I RCCGGY 2 cut(s) 812, 1301
Bse1I ACTGG 3 cut(s) 564, 1057, 1336
BseBI CCWGG 3 cut(s) 683, 912, 945
BseDI CCNNGG 3 cut(s) 496, 537, 1041
BseGI GGATG 4 cut(s) 39, 578, 691, 1328
BseLI CCNNNNNNNGG 3 cut(s) 944, 1041, 1404
BseMII CTCAG 2 cut(s) 687, 1085
BseNI ACTGG 3 cut(s) 564, 1057, 1336
BseXI GCAGC 4 cut(s) 268, 536, 688, 1402
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 4 cut(s) 111, 168, 247, 542
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 5 cut(s) 334, 498, 813, 1041, 1302
BslFI GGGAC 1 cut(s) 812
BslI CCNNNNNNNGG 3 cut(s) 944, 1041, 1404
BsmAI GTCTC 2 cut(s) 436, 960
BsmFI GGGAC 1 cut(s) 812
BsnI GGCC 4 cut(s) 111, 168, 247, 542
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 2 cut(s) 48, 1093
Bsp1286I GDGCHC 2 cut(s) 376, 1377
Bsp143I GATC 3 cut(s) 116, 424, 1422
BspACI CCGC 8 cut(s) 510, 522, 528, 537, 539, 704, 1317, 1395
BspANI GGCC 4 cut(s) 111, 168, 247, 542
BspCNI CTCAG 2 cut(s) 686, 1084
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 1 cut(s) 502
BspMI ACCTGC 1 cut(s) 503
BspT104I TTCGAA 2 cut(s) 48, 1093
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 530
BsrFI RCCGGY 2 cut(s) 812, 1301
BsrI ACTGG 3 cut(s) 564, 1057, 1336
BssAI RCCGGY 2 cut(s) 812, 1301
BssECI CCNNGG 3 cut(s) 496, 537, 1041
BssMI GATC 3 cut(s) 116, 424, 1422
Bst2UI CCWGG 3 cut(s) 683, 912, 945
Bst4CI ACNGT 6 cut(s) 179, 971, 984, 1081, 1215, 1247
Bst6I CTCTTC 2 cut(s) 490, 496
BstBI TTCGAA 2 cut(s) 48, 1093
BstC8I GCNNGC 2 cut(s) 156, 814
BstDEI CTNAG 2 cut(s) 673, 1071
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 4 cut(s) 39, 578, 691, 1328
BstFNI CGCG 1 cut(s) 539
BstHHI GCGC 1 cut(s) 1307
BstKTI GATC 3 cut(s) 119, 427, 1425
BstMAI GTCTC 2 cut(s) 436, 960
BstMBI GATC 3 cut(s) 116, 424, 1422
BstMWI GCNNNNNNNGC 5 cut(s) 518, 527, 533, 536, 830
BstNI CCWGG 3 cut(s) 683, 912, 945
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 5 cut(s) 496, 681, 910, 943, 1040
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 4 cut(s) 268, 536, 688, 1402
BstX2I RGATCY 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 116
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 4 cut(s) 111, 168, 247, 542
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 4 cut(s) 39, 578, 691, 1328
BtsIMutI CAGTG 2 cut(s) 80, 1064
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 2 cut(s) 156, 814
CaiI CAGNNNCTG 1 cut(s) 1415
CfoI GCGC 1 cut(s) 1307
Cfr10I RCCGGY 2 cut(s) 812, 1301
Cfr13I GGNCC 1 cut(s) 1319
Cfr42I CCGCGG 1 cut(s) 540
Csp6I GTAC 1 cut(s) 628
CviAII CATG 3 cut(s) 391, 1156, 1367
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 2 cut(s) 673, 1071
DpnI GATC 3 cut(s) 118, 426, 1424
DpnII GATC 3 cut(s) 116, 424, 1422
EaeI YGGCCR 1 cut(s) 245
Eam1104I CTCTTC 2 cut(s) 490, 496
EarI CTCTTC 2 cut(s) 490, 496
EciI GGCGGA 1 cut(s) 1332
Eco24I GRGCYC 1 cut(s) 1377
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 1319
EcoRII CCWGG 3 cut(s) 681, 910, 943
EcoT38I GRGCYC 1 cut(s) 1377
FaeI CATG 3 cut(s) 394, 1159, 1370
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 1 cut(s) 812
FatI CATG 3 cut(s) 390, 1155, 1366
FbaI TGATCA 1 cut(s) 1422
FblI GTMKAC 3 cut(s) 338, 383, 1067
FokI GGATG 4 cut(s) 46, 565, 698, 1315
FriOI GRGCYC 1 cut(s) 1377
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 1306
HaeIII GGCC 4 cut(s) 111, 168, 247, 542
HapII CCGG 5 cut(s) 334, 498, 813, 1041, 1302
HhaI GCGC 1 cut(s) 1307
Hin1II CATG 3 cut(s) 394, 1159, 1370
Hin6I GCGC 1 cut(s) 1305
HinP1I GCGC 1 cut(s) 1305
HincII GTYRAC 3 cut(s) 316, 384, 1068
HindII GTYRAC 3 cut(s) 316, 384, 1068
HindIII AAGCTT 3 cut(s) 52, 645, 822
HinfI GANTC 4 cut(s) 161, 566, 887, 1069
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 5 cut(s) 334, 498, 813, 1041, 1302
HphI GGTGA 3 cut(s) 236, 527, 1069
Hpy166II GTNNAC 6 cut(s) 125, 316, 339, 384, 1068, 1077
Hpy188I TCNGA 6 cut(s) 175, 424, 505, 838, 1333, 1422
Hpy188III TCNNGA 2 cut(s) 65, 130
Hpy8I GTNNAC 6 cut(s) 125, 316, 339, 384, 1068, 1077
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 2 cut(s) 101, 667
HpyCH4III ACNGT 6 cut(s) 179, 971, 984, 1081, 1215, 1247
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 6 cut(s) 80, 182, 284, 902, 1297, 1370
HpyF10VI GCNNNNNNNGC 5 cut(s) 518, 527, 533, 536, 830
HpyF3I CTNAG 2 cut(s) 673, 1071
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 3 cut(s) 394, 1159, 1370
HspAI GCGC 1 cut(s) 1305
KroI GCCGGC 1 cut(s) 812
KroNI GCCGGC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 1422
KspAI GTTAAC 1 cut(s) 316
KspI CCGCGG 1 cut(s) 540
Kzo9I GATC 3 cut(s) 116, 424, 1422
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 4 cut(s) 268, 536, 688, 1402
LweI GCATC 5 cut(s) 67, 191, 208, 842, 897
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 224, 737, 988, 1107
MalI GATC 3 cut(s) 118, 426, 1424
MbiI CCGCTC 1 cut(s) 530
MboI GATC 3 cut(s) 116, 424, 1422
MboII GAAGA 4 cut(s) 461, 477, 483, 1422
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 376, 1377
MlsI TGGCCA 1 cut(s) 247
MluNI TGGCCA 1 cut(s) 247
MlyI GAGTC 1 cut(s) 1063
MmeI TCCRAC 2 cut(s) 1141, 1356
MnlI CCTC 8 cut(s) 200, 377, 499, 553, 626, 1193, 1200, 1247
Mox20I TGGCCA 1 cut(s) 247
MreI CGCCGGCG 1 cut(s) 812
MroNI GCCGGC 1 cut(s) 812
MscI TGGCCA 1 cut(s) 247
MseI TTAA 6 cut(s) 26, 147, 315, 743, 873, 933
Msp20I TGGCCA 1 cut(s) 247
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 5 cut(s) 334, 498, 813, 1041, 1302
MspR9I CCNGG 5 cut(s) 498, 683, 912, 945, 1042
MunI CAATTG 1 cut(s) 1308
MvaI CCWGG 3 cut(s) 683, 912, 945
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 5 cut(s) 518, 527, 533, 536, 830
NaeI GCCGGC 1 cut(s) 814
NciI CCSGG 2 cut(s) 498, 1042
NdeII GATC 3 cut(s) 116, 424, 1422
NgoMIV GCCGGC 1 cut(s) 812
NlaIII CATG 3 cut(s) 394, 1159, 1370
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 224, 1107
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 2 cut(s) 48, 1093
PaqCI CACCTGC 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 1211
PdiI GCCGGC 1 cut(s) 814
PfeI GAWTC 3 cut(s) 161, 566, 887
PflMI CCANNNNNTGG 1 cut(s) 944
PleI GAGTC 1 cut(s) 1063
PpsI GAGTC 1 cut(s) 1063
PsiI TTATAA 1 cut(s) 755
Psp6I CCWGG 3 cut(s) 681, 910, 943
PspGI CCWGG 3 cut(s) 681, 910, 943
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 1 cut(s) 1319
PstNI CAGNNNCTG 1 cut(s) 1415
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SacII CCGCGG 1 cut(s) 540
SalI GTCGAC 2 cut(s) 382, 1066
SaqAI TTAA 6 cut(s) 26, 147, 315, 743, 873, 933
Sau3AI GATC 3 cut(s) 116, 424, 1422
Sau96I GGNCC 1 cut(s) 1319
SchI GAGTC 1 cut(s) 1063
ScrFI CCNGG 5 cut(s) 498, 683, 912, 945, 1042
SduI GDGCHC 2 cut(s) 376, 1377
SfaNI GCATC 5 cut(s) 67, 191, 208, 842, 897
SfcI CTRYAG 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 540
SfuI TTCGAA 2 cut(s) 48, 1093
SgrAI CRCCGGYG 1 cut(s) 812
SgrBI CCGCGG 1 cut(s) 540
SinI GGWCC 1 cut(s) 1319
SsiI CCGC 8 cut(s) 510, 522, 528, 537, 539, 704, 1317, 1395
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 5 cut(s) 496, 681, 910, 943, 1040
TaaI ACNGT 6 cut(s) 179, 971, 984, 1081, 1215, 1247
TaiI ACGT 1 cut(s) 383
TaqI TCGA 5 cut(s) 48, 383, 1067, 1093, 1242
TaqII GACCGA 1 cut(s) 893
TatI WGTACW 1 cut(s) 627
TauI GCSGC 7 cut(s) 512, 524, 530, 539, 542, 707, 1397
TfiI GAWTC 3 cut(s) 161, 566, 887
Tru1I TTAA 6 cut(s) 26, 147, 315, 743, 873, 933
Tru9I TTAA 6 cut(s) 26, 147, 315, 743, 873, 933
TscAI CASTG 2 cut(s) 87, 1064
TseFI GTSAC 2 cut(s) 224, 1107
TseI GCWGC 4 cut(s) 281, 524, 701, 1415
Tsp45I GTSAC 2 cut(s) 224, 1107
TspDTI ATGAA 5 cut(s) 567, 702, 801, 1251, 1317
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 2 cut(s) 87, 1064
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 1319
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 3 cut(s) 338, 383, 1067
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.