Rh7DG159900

cellulase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010905
Physical Location & Seq
Reverse (-)
14225164 .. 14228288
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG159900.1

Sequence Viewer

Length: 1248 bp
ATGCAGGTAAATTTGACTGGAGGATACTATGATGCAGGCGACAACGTGAAGTTCGTGTGGCCAATGGCATATAGTGTGACCTTGTTGAGTTGGAGTCTCATTGAGTACCAGCAGCAGATATCTTCAGCAAACCAGCTTGCATACGTCCAGAGAGCAATCCGCTGGGGCACAGACTTCATAATAAAAGCTCACCTTTCGCCCACCACCTTCTATACTCAGGTAGGAGATGGAAATGTGGATCACCAGTGCTGGGAGCGTCCCGAAGACATGGACTCATCACGAAAGCTCTACAAGATCACTTCGAGTTCTCCAGGCAGTGAGGCCGCAGCTGAGGCTGCTGCTGCCCTCGCTGCAGCCTCAATTGCCTTTAAGACAGTTGACTCCACCTATTCGGCAAAGCTCTCAAGTCATTCCCAATCTCTGTTTGACTTGGCTGACAAGTATAGAGGGTCTTACCAAGTCTCTTGCCCTTTCTACTGCTCCTACTCTGGCTACCAGGATGAGCTGTTATGGGCTGCGGCTTGGCTATACAAGGCAAGTACTGGAAACAGAAGCTATTTTAGTTATGTGGTAAACAACCAAGGATGGAGTCAAGCAATGTCTGAGTTTAGTTGGGATAACAAATTTGTTGGAGCTCAGACTTTACTAGCCAAGGAATACCATGCTGGAAATACTGATTTGCTCAAGTACAAGACCAATGCGGAGTCATTTATATGTGCAGTGATGCCAGGGAGTAGCTCTACACAAGTTCAAAGAACACCCGGTGGACTTCTGTTTATTAGAGACAGTAGTAACTTGCAGTATGTTACAAGCTCTACCTTGGTGCTATTCATCTACTCAAAAACCATAAGTAGTACACAAACTGGTGGAGTTCAATGCGGCTCCACGTTTTTCTCTGCTGCGAAAATCAAAGCCTTTGCTAAAGCTCAGGTGGACTACATCCTTGGAGCCAACCCCAAGCAGATGTCATACATGGTGGGTTTTGGTACCACATACCCAACAAAATTGCACCATAGAGGTTCATCCATCCCTTCAATCAAGACCCTCCACACCAAGGTGGACTGCAATGGTGGCTATACCTATTACAATTCTGGGAGTCCAAATCCAAATACTCATGTGGGTGCAATTGTTGGGGGTCCTGATGTGAATGACCAGTTCCAAAATGCAAGATCAGACTATTCTCATTCTGAACCTACAACTTATATCAATGCAGCTTTTGTGGGATCAGCAGCTGCTTTACTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

415

Amino Acids

45.38

Weight (kDa)

7.6

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 2 - 408 6.5e-137 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 986
AccB1I GGYRCC 1 cut(s) 986
AciI CCGC 5 cut(s) 160, 324, 518, 701, 879
AclWI GGATC 2 cut(s) 246, 1231
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 10, 623
AcuI CTGAAG 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 5 cut(s) 107, 541, 689, 856, 988
AfiI CCNNNNNNNGG 2 cut(s) 250, 1054
AgsI TTSAA 3 cut(s) 752, 875, 1035
AjnI CCWGG 3 cut(s) 310, 495, 727
AleI CACNNNNGTG 1 cut(s) 1055
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 3 cut(s) 101, 466, 777
AlwI GGATC 2 cut(s) 246, 1231
AlwNI CAGNNNCTG 1 cut(s) 1232
AoxI GGCC 2 cut(s) 59, 321
ApoI RAATTY 2 cut(s) 10, 623
Asp718I GGTACC 1 cut(s) 986
AspS9I GGNCC 1 cut(s) 1136
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 2 cut(s) 182, 233
AvaII GGWCC 1 cut(s) 1136
BaeGI GKGCMC 1 cut(s) 170
BalI TGGCCA 1 cut(s) 61
BanI GGYRCC 1 cut(s) 986
BanII GRGCYC 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 637
BbvCI CCTCAGC 1 cut(s) 330
BccI CCATC 3 cut(s) 221, 579, 1034
BciT130I CCWGG 3 cut(s) 312, 497, 729
BciVI GTATCC 1 cut(s) 17
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 3 cut(s) 101, 466, 777
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 17
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 4 cut(s) 312, 497, 729, 762
Bme18I GGWCC 1 cut(s) 1136
BmgT120I GGNCC 1 cut(s) 1136
BmiI GGNNCC 4 cut(s) 883, 949, 988, 1137
BmrFI CCNGG 4 cut(s) 312, 497, 729, 762
BmsI GCATC 2 cut(s) 22, 714
BpiI GAAGAC 1 cut(s) 270
BpmI CTGGAG 2 cut(s) 39, 294
Bpu10I CCTNAGC 2 cut(s) 330, 927
BpuEI CTTGAG 2 cut(s) 388, 668
BpuMI CCSGG 1 cut(s) 762
BsaJI CCNNGG 6 cut(s) 580, 651, 728, 819, 943, 1053
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 1054
Bse1I ACTGG 5 cut(s) 22, 244, 547, 868, 1153
Bse3DI GCAATG 2 cut(s) 603, 1072
BseBI CCWGG 3 cut(s) 312, 497, 729
BseDI CCNNGG 6 cut(s) 580, 651, 728, 819, 943, 1053
BseGI GGATG 5 cut(s) 505, 590, 939, 1022, 1026
BseLI CCNNNNNNNGG 2 cut(s) 250, 1054
BseMI GCAATG 2 cut(s) 603, 1072
BseMII CTCAG 5 cut(s) 230, 321, 594, 650, 941
BseNI ACTGG 5 cut(s) 22, 244, 547, 868, 1153
BseSI GKGCMC 1 cut(s) 170
BseYI CCCAGC 2 cut(s) 162, 249
BsgI GTGCAG 1 cut(s) 738
BshFI GGCC 2 cut(s) 61, 323
BshNI GGYRCC 1 cut(s) 986
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 1 cut(s) 762
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 2 cut(s) 250, 1054
BsmAI GTCTC 3 cut(s) 101, 466, 777
BsmFI GGGAC 1 cut(s) 243
BsnI GGCC 2 cut(s) 61, 323
Bsp1286I GDGCHC 2 cut(s) 170, 637
Bsp143I GATC 4 cut(s) 238, 294, 1169, 1223
BspACI CCGC 5 cut(s) 160, 324, 518, 701, 879
BspANI GGCC 2 cut(s) 61, 323
BspCNI CTCAG 5 cut(s) 229, 322, 595, 649, 940
BspLI GGNNCC 4 cut(s) 883, 949, 988, 1137
BspMAI CTGCAG 1 cut(s) 355
BspPI GGATC 2 cut(s) 246, 1231
BspT107I GGYRCC 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 603, 1072
BsrI ACTGG 5 cut(s) 22, 244, 547, 868, 1153
BssECI CCNNGG 6 cut(s) 580, 651, 728, 819, 943, 1053
BssMI GATC 4 cut(s) 238, 294, 1169, 1223
BssT1I CCWWGG 5 cut(s) 580, 651, 819, 943, 1053
Bst2UI CCWGG 3 cut(s) 312, 497, 729
Bst4CI ACNGT 2 cut(s) 376, 788
BstC8I GCNNGC 2 cut(s) 37, 138
BstDEI CTNAG 5 cut(s) 216, 330, 603, 636, 927
BstF5I GGATG 5 cut(s) 505, 590, 939, 1022, 1026
BstKTI GATC 4 cut(s) 241, 297, 1172, 1226
BstMAI GTCTC 3 cut(s) 101, 466, 777
BstMBI GATC 4 cut(s) 238, 294, 1169, 1223
BstMWI GCNNNNNNNGC 8 cut(s) 332, 335, 341, 347, 350, 362, 1071, 1241
BstNI CCWGG 3 cut(s) 312, 497, 729
BstSCI CCNGG 4 cut(s) 310, 495, 727, 760
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 170
BstV2I GAAGAC 1 cut(s) 270
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 2 cut(s) 61, 323
BtsCI GGATG 5 cut(s) 505, 590, 939, 1022, 1026
BtsI GCAGTG 2 cut(s) 322, 726
BtsIMutI CAGTG 3 cut(s) 251, 322, 726
Cac8I GCNNGC 2 cut(s) 37, 138
CaiI CAGNNNCTG 1 cut(s) 1232
Cfr13I GGNCC 1 cut(s) 1136
CseI GACGC 1 cut(s) 245
Csp6I GTAC 5 cut(s) 106, 540, 688, 855, 987
CviAII CATG 4 cut(s) 268, 662, 973, 1115
CviQI GTAC 5 cut(s) 106, 540, 688, 855, 987
DdeI CTNAG 5 cut(s) 216, 330, 603, 636, 927
DpnI GATC 4 cut(s) 240, 296, 1171, 1225
DpnII GATC 4 cut(s) 238, 294, 1169, 1223
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 635
Eco130I CCWWGG 5 cut(s) 580, 651, 819, 943, 1053
Eco24I GRGCYC 1 cut(s) 637
Eco32I GATATC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 1136
Eco53kI GAGCTC 1 cut(s) 635
Eco57I CTGAAG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 1136
EcoRII CCWGG 3 cut(s) 310, 495, 727
EcoRV GATATC 1 cut(s) 120
EcoT14I CCWWGG 5 cut(s) 580, 651, 819, 943, 1053
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 5 cut(s) 580, 651, 819, 943, 1053
FaeI CATG 4 cut(s) 271, 665, 976, 1118
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 1 cut(s) 243
FatI CATG 4 cut(s) 267, 661, 972, 1114
FokI GGATG 5 cut(s) 512, 597, 926, 1009, 1013
FriOI GRGCYC 1 cut(s) 637
FspBI CTAG 1 cut(s) 647
GsaI CCCAGC 2 cut(s) 166, 253
GsuI CTGGAG 2 cut(s) 39, 294
HaeIII GGCC 2 cut(s) 61, 323
HapII CCGG 1 cut(s) 762
HgaI GACGC 1 cut(s) 245
Hin1II CATG 4 cut(s) 271, 665, 976, 1118
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 6 cut(s) 94, 272, 380, 589, 704, 1096
HpaII CCGG 1 cut(s) 762
HphI GGTGA 2 cut(s) 182, 233
Hpy166II GTNNAC 6 cut(s) 379, 574, 767, 857, 934, 1060
Hpy188I TCNGA 4 cut(s) 604, 639, 1174, 1189
Hpy188III TCNNGA 5 cut(s) 148, 260, 279, 1039, 1139
Hpy8I GTNNAC 6 cut(s) 379, 574, 767, 857, 934, 1060
HpyAV CCTTC 2 cut(s) 217, 1041
HpyCH4III ACNGT 2 cut(s) 376, 788
HpyCH4IV ACGT 3 cut(s) 45, 144, 887
HpyF10VI GCNNNNNNNGC 8 cut(s) 332, 335, 341, 347, 350, 362, 1071, 1241
HpyF3I CTNAG 5 cut(s) 216, 330, 603, 636, 927
HpySE526I ACGT 3 cut(s) 45, 144, 887
Hsp92II CATG 4 cut(s) 271, 665, 976, 1118
KpnI GGTACC 1 cut(s) 990
Kzo9I GATC 4 cut(s) 238, 294, 1169, 1223
LmnI GCTCC 5 cut(s) 253, 485, 632, 887, 947
LweI GCATC 2 cut(s) 22, 714
MaeI CTAG 1 cut(s) 647
MaeII ACGT 3 cut(s) 45, 144, 887
MaeIII GTNAC 3 cut(s) 76, 791, 805
MalI GATC 4 cut(s) 240, 296, 1171, 1225
MboI GATC 4 cut(s) 238, 294, 1169, 1223
MboII GAAGA 2 cut(s) 114, 275
MfeI CAATTG 2 cut(s) 360, 1125
MhlI GDGCHC 2 cut(s) 170, 637
MlsI TGGCCA 1 cut(s) 61
MluCI AATT 6 cut(s) 10, 360, 623, 1004, 1087, 1125
MluNI TGGCCA 1 cut(s) 61
MlyI GAGTC 6 cut(s) 103, 266, 374, 598, 713, 1105
MmeI TCCRAC 2 cut(s) 71, 610
MnlI CCTC 8 cut(s) 14, 313, 325, 356, 367, 440, 1010, 1055
Mox20I TGGCCA 1 cut(s) 61
MscI TGGCCA 1 cut(s) 61
MseI TTAA 2 cut(s) 369, 1246
MslI CAYNNNNRTG 3 cut(s) 712, 1055, 1119
Msp20I TGGCCA 1 cut(s) 61
MspA1I CMGCKG 3 cut(s) 162, 329, 1232
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 4 cut(s) 312, 497, 729, 762
MunI CAATTG 2 cut(s) 360, 1125
MvaI CCWGG 3 cut(s) 312, 497, 729
MwoI GCNNNNNNNGC 8 cut(s) 332, 335, 341, 347, 350, 362, 1071, 1241
NciI CCSGG 1 cut(s) 762
NdeII GATC 4 cut(s) 238, 294, 1169, 1223
NlaIII CATG 4 cut(s) 271, 665, 976, 1118
NlaIV GGNNCC 4 cut(s) 883, 949, 988, 1137
NmuCI GTSAC 1 cut(s) 76
OliI CACNNNNGTG 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PleI GAGTC 6 cut(s) 102, 266, 374, 597, 712, 1104
PpsI GAGTC 6 cut(s) 102, 266, 374, 597, 712, 1104
PpuMI RGGWCCY 1 cut(s) 1136
Psp124BI GAGCTC 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 1136
Psp6I CCWGG 3 cut(s) 310, 495, 727
PspFI CCCAGC 2 cut(s) 162, 249
PspGI CCWGG 3 cut(s) 310, 495, 727
PspN4I GGNNCC 4 cut(s) 883, 949, 988, 1137
PspPI GGNCC 1 cut(s) 1136
PspPPI RGGWCCY 1 cut(s) 1136
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 1232
PvuII CAGCTG 2 cut(s) 329, 1232
RsaI GTAC 5 cut(s) 107, 541, 689, 856, 988
RsaNI GTAC 5 cut(s) 106, 540, 688, 855, 987
RseI CAYNNNNRTG 3 cut(s) 712, 1055, 1119
SacI GAGCTC 1 cut(s) 637
SaqAI TTAA 2 cut(s) 369, 1246
Sau3AI GATC 4 cut(s) 238, 294, 1169, 1223
Sau96I GGNCC 1 cut(s) 1136
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 6 cut(s) 103, 266, 374, 598, 713, 1105
ScrFI CCNGG 4 cut(s) 312, 497, 729, 762
SduI GDGCHC 2 cut(s) 170, 637
SfaNI GCATC 2 cut(s) 22, 714
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 1 cut(s) 1136
SmiMI CAYNNNNRTG 3 cut(s) 712, 1055, 1119
SmlI CTYRAG 2 cut(s) 403, 683
SmoI CTYRAG 2 cut(s) 403, 683
Sse9I AATT 6 cut(s) 10, 360, 623, 1004, 1087, 1125
SsiI CCGC 5 cut(s) 160, 324, 518, 701, 879
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 637
StyD4I CCNGG 4 cut(s) 310, 495, 727, 760
StyI CCWWGG 5 cut(s) 580, 651, 819, 943, 1053
TaaI ACNGT 2 cut(s) 376, 788
TaiI ACGT 3 cut(s) 48, 147, 890
TaqI TCGA 1 cut(s) 302
TasI AATT 6 cut(s) 10, 360, 623, 1004, 1087, 1125
TatI WGTACW 3 cut(s) 539, 687, 854
TauI GCSGC 3 cut(s) 326, 521, 882
Tru1I TTAA 2 cut(s) 369, 1246
Tru9I TTAA 2 cut(s) 369, 1246
TscAI CASTG 3 cut(s) 251, 322, 726
TseFI GTSAC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 3 cut(s) 166, 820, 1011
TspRI CASTG 3 cut(s) 251, 322, 726
VpaK11BI GGWCC 1 cut(s) 1136
XapI RAATTY 2 cut(s) 10, 623
XspI CTAG 1 cut(s) 647
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.