Rroxscaffold_3G00259210

cellulase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010905
Physical Location & Seq
Forward (+)
53613652 .. 53615993
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259210.1

Sequence Viewer

Length: 1434 bp
ATGGGGCATGGTAGAGTTGTTGCATGTGTTTTGGTATTGTGGTGTTTTTTGAACGAGATAGTCTCAGCCATTGACTATGAAGATGCGCTTGGGAAATCTATATTGTTCTTCGAAGGCCAACGCTCCGGGAAGTTGCCGACCACCCAGCGAGTATCTTGGAGAGGAGACTCTGCACTTTCCGATGGCCAGCTTGAAGGAGTAAATTTGACTGGAGGATACTATGATGCAGGCGACAACGTGAAGTTCGTGTGGCTGATGGCATATAGTGTGAGCTTGTTGAGTTGGAGTCTCATTGAGTACCAGCAGCAGATATCTTCAGCAAACCAGCTTGCATACGTCCAGAGAGCAATCCGCTGGGGCACAGACTTCATAATGAAAGCTCACCTTTTCGCCCACCACCTTCTACACTCAGGAGATGGAAATGTGGATCACCAGTGCTGGGAGCGTCCCGAAGACATGGACACGTCACGAAAGCTCTACAAGATCACTTCGAGTTCTCCAGGCAGTGAGGCCGCAGCTGAGGCTGCTGCTGCCCTCGCTGCAGCCTCAATTGTCTTTATGACAGTTGACTCCACCTATTCGGCAAAGCTCTCAAGTCATTCCCAATCTCTGTTTGACTTGGCTGACAAGTATAGAGGGTCTTACCAAGTCTCTTGCCCTTTCTACTGCTCCTACTCTGGCTACCAGGATGAGCTGTTATGGGCTGCGGCTTGGCTATACAAGGCAAGTGGAAACAGAAGCTATTTTAGTTATGTGGTAAACAACCAAGGATGGAGTCAGGCAATGTCTGAGTTCAGTTGGGATAACAAATTTGTTGGAGCTCAGACTTTACTAGCCAAGGAATACCATGCTGGAAATACTGATTTGCTCAAGTACAAGAACAATGCGGAGTCATTTATATGTGCAGTGATGCCAGGGAGTAGCTCTACACAAGTTCAAAGAACACCCGGTGGACTTCTGTTTGTTAGAGACAGTAGTAACTTGCAGTATGTTACAAGCTCTACCTTGGTGCTATTCATCTACTCAAAAACCATAAGTAGTACAAAAACTGGTGGAGTTCAATGCGGCTCCACGTTTTTCTCTGCTGCGAAAATCAAAGCCTTTGCTAAAGCTCAGGTGGACTACATCCTTGGAGCCAACCCCAAGCAGATGTCATACATGGTGGGTTTTGGTACCACATACCCAACAAAATTGCACCATAGAGGTTCATCCATCCCTTCAATCAAGACCCTCCACACCGAGGTGGACTGCAATGGTGGCTATACCTATTACAATTCTGGGAGTCCAAATCCAAATACTCATGTAGGTGCAATTGTTGGGGGTCCTGATGTGAATGACCAGTTCCAGAATGCAAGATCAGACTATTCTCATTCTGAACCTACAACTTATATCAATGCAGCTTTTGTCGGATCAGCAGCTGCTTTACTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

477

Amino Acids

52.21

Weight (kDa)

6.49

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 26 - 470 2.4e-149 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1172
AccB1I GGYRCC 1 cut(s) 1172
AciI CCGC 5 cut(s) 352, 513, 707, 887, 1065
AclWI GGATC 2 cut(s) 435, 1417
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 2 cut(s) 202, 809
AcuI CTGAAG 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 950
AfaI GTAC 4 cut(s) 299, 875, 1042, 1174
AfiI CCNNNNNNNGG 2 cut(s) 439, 1240
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 5 cut(s) 52, 194, 938, 1061, 1221
AjiI CACGTC 1 cut(s) 465
AjnI CCWGG 3 cut(s) 499, 684, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AleI CACNNNNGTG 1 cut(s) 1241
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 5 cut(s) 67, 159, 293, 655, 963
AlwI GGATC 2 cut(s) 435, 1417
AlwNI CAGNNNCTG 1 cut(s) 1418
AoxI GGCC 3 cut(s) 115, 184, 510
ApoI RAATTY 2 cut(s) 202, 809
Asp718I GGTACC 1 cut(s) 1172
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 1322
AsuC2I CCSGG 2 cut(s) 127, 948
AsuHPI GGTGA 2 cut(s) 374, 422
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 1322
BaeGI GKGCMC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 1172
BanII GRGCYC 1 cut(s) 823
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 823
BbvCI CCTCAGC 1 cut(s) 519
BccI CCATC 5 cut(s) 176, 250, 410, 765, 1220
BciT130I CCWGG 3 cut(s) 501, 686, 915
BciVI GTATCC 1 cut(s) 209
BcnI CCSGG 2 cut(s) 127, 948
BcoDI GTCTC 5 cut(s) 67, 159, 293, 655, 963
BfaI CTAG 1 cut(s) 833
BfmI CTRYAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 209
Bme1390I CCNGG 5 cut(s) 127, 501, 686, 915, 948
Bme18I GGWCC 1 cut(s) 1322
BmgBI CACGTC 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 1322
BmiI GGNNCC 4 cut(s) 1069, 1135, 1174, 1323
BmrFI CCNGG 5 cut(s) 127, 501, 686, 915, 948
BmsI GCATC 3 cut(s) 73, 214, 900
BpiI GAAGAC 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpmI CTGGAG 2 cut(s) 231, 483
Bpu10I CCTNAGC 2 cut(s) 519, 1113
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 577, 854
BpuMI CCSGG 2 cut(s) 127, 948
BsaJI CCNNGG 6 cut(s) 766, 837, 914, 1005, 1129, 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 1240
Bse1I ACTGG 4 cut(s) 214, 433, 1054, 1339
Bse3DI GCAATG 2 cut(s) 789, 1258
BseBI CCWGG 3 cut(s) 501, 686, 915
BseDI CCNNGG 6 cut(s) 766, 837, 914, 1005, 1129, 1239
BseGI GGATG 5 cut(s) 694, 776, 1125, 1208, 1212
BseLI CCNNNNNNNGG 2 cut(s) 439, 1240
BseMI GCAATG 2 cut(s) 789, 1258
BseMII CTCAG 6 cut(s) 78, 423, 510, 780, 836, 1127
BseNI ACTGG 4 cut(s) 214, 433, 1054, 1339
BseRI GAGGAG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 362
BseYI CCCAGC 3 cut(s) 144, 354, 438
BsgI GTGCAG 2 cut(s) 156, 924
BshFI GGCC 3 cut(s) 117, 186, 512
BshNI GGYRCC 1 cut(s) 1172
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 2 cut(s) 126, 948
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 439, 1240
BsmAI GTCTC 5 cut(s) 67, 159, 293, 655, 963
BsmFI GGGAC 1 cut(s) 432
BsmI GAATGC 1 cut(s) 1354
BsnI GGCC 3 cut(s) 117, 186, 512
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 362, 823
Bsp143I GATC 4 cut(s) 427, 483, 1355, 1409
BspACI CCGC 5 cut(s) 352, 513, 707, 887, 1065
BspANI GGCC 3 cut(s) 117, 186, 512
BspCNI CTCAG 6 cut(s) 77, 422, 511, 781, 835, 1126
BspLI GGNNCC 4 cut(s) 1069, 1135, 1174, 1323
BspMAI CTGCAG 1 cut(s) 544
BspPI GGATC 2 cut(s) 435, 1417
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 1172
BsrDI GCAATG 2 cut(s) 789, 1258
BsrI ACTGG 4 cut(s) 214, 433, 1054, 1339
BssECI CCNNGG 6 cut(s) 766, 837, 914, 1005, 1129, 1239
BssMI GATC 4 cut(s) 427, 483, 1355, 1409
BssT1I CCWWGG 4 cut(s) 766, 837, 1005, 1129
Bst2UI CCWGG 3 cut(s) 501, 686, 915
Bst4CI ACNGT 2 cut(s) 565, 974
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 188, 229, 330
BstDEI CTNAG 6 cut(s) 64, 409, 519, 789, 822, 1113
BstF5I GGATG 5 cut(s) 694, 776, 1125, 1208, 1212
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 430, 486, 1358, 1412
BstMAI GTCTC 5 cut(s) 67, 159, 293, 655, 963
BstMBI GATC 4 cut(s) 427, 483, 1355, 1409
BstMWI GCNNNNNNNGC 7 cut(s) 521, 524, 530, 536, 539, 1257, 1427
BstNI CCWGG 3 cut(s) 501, 686, 915
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 5 cut(s) 125, 499, 684, 913, 946
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 459
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 117, 186, 512
BtrI CACGTC 1 cut(s) 465
BtsCI GGATG 5 cut(s) 694, 776, 1125, 1208, 1212
BtsI GCAGTG 2 cut(s) 511, 912
BtsIMutI CAGTG 3 cut(s) 440, 511, 912
Cac8I GCNNGC 3 cut(s) 188, 229, 330
CaiI CAGNNNCTG 1 cut(s) 1418
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 1322
CseI GACGC 1 cut(s) 434
Csp6I GTAC 4 cut(s) 298, 874, 1041, 1173
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviAII CATG 6 cut(s) 8, 24, 457, 848, 1159, 1301
CviQI GTAC 4 cut(s) 298, 874, 1041, 1173
DdeI CTNAG 6 cut(s) 64, 409, 519, 789, 822, 1113
DpnI GATC 4 cut(s) 429, 485, 1357, 1411
DpnII GATC 4 cut(s) 427, 483, 1355, 1409
DraIII CACNNNGTG 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 821
Eco130I CCWWGG 4 cut(s) 766, 837, 1005, 1129
Eco24I GRGCYC 1 cut(s) 823
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 1322
Eco53kI GAGCTC 1 cut(s) 821
Eco57I CTGAAG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 821
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRII CCWGG 3 cut(s) 499, 684, 913
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 4 cut(s) 766, 837, 1005, 1129
EcoT38I GRGCYC 1 cut(s) 823
ErhI CCWWGG 4 cut(s) 766, 837, 1005, 1129
FaeI CATG 6 cut(s) 11, 27, 460, 851, 1162, 1304
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 369, 401
FaqI GGGAC 1 cut(s) 432
FatI CATG 6 cut(s) 7, 23, 456, 847, 1158, 1300
FokI GGATG 5 cut(s) 701, 783, 1112, 1195, 1199
FriOI GRGCYC 1 cut(s) 823
FspBI CTAG 1 cut(s) 833
GlaI GCGC 1 cut(s) 87
GsaI CCCAGC 3 cut(s) 148, 358, 442
GsuI CTGGAG 2 cut(s) 231, 483
HaeIII GGCC 3 cut(s) 117, 186, 512
HapII CCGG 2 cut(s) 126, 948
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 88
Hin1II CATG 6 cut(s) 11, 27, 460, 851, 1162, 1304
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 6 cut(s) 167, 286, 569, 775, 890, 1282
HpaII CCGG 2 cut(s) 126, 948
HphI GGTGA 2 cut(s) 374, 422
Hpy166II GTNNAC 5 cut(s) 568, 760, 953, 1120, 1246
Hpy188I TCNGA 6 cut(s) 181, 790, 825, 1360, 1375, 1409
Hpy188III TCNNGA 7 cut(s) 340, 411, 449, 468, 1225, 1325, 1345
Hpy8I GTNNAC 5 cut(s) 568, 760, 953, 1120, 1246
HpyAV CCTTC 4 cut(s) 107, 188, 410, 1227
HpyCH4III ACNGT 2 cut(s) 565, 974
HpyCH4IV ACGT 4 cut(s) 237, 336, 464, 1073
HpyF10VI GCNNNNNNNGC 7 cut(s) 521, 524, 530, 536, 539, 1257, 1427
HpyF3I CTNAG 6 cut(s) 64, 409, 519, 789, 822, 1113
HpySE526I ACGT 4 cut(s) 237, 336, 464, 1073
Hsp92II CATG 6 cut(s) 11, 27, 460, 851, 1162, 1304
HspAI GCGC 1 cut(s) 86
KpnI GGTACC 1 cut(s) 1176
Kzo9I GATC 4 cut(s) 427, 483, 1355, 1409
LmnI GCTCC 6 cut(s) 128, 442, 674, 818, 1073, 1133
LweI GCATC 3 cut(s) 73, 214, 900
MaeI CTAG 1 cut(s) 833
MaeII ACGT 4 cut(s) 237, 336, 464, 1073
MaeIII GTNAC 3 cut(s) 465, 977, 991
MalI GATC 4 cut(s) 429, 485, 1357, 1411
MboI GATC 4 cut(s) 427, 483, 1355, 1409
MboII GAAGA 4 cut(s) 92, 100, 306, 464
MfeI CAATTG 2 cut(s) 549, 1311
MhlI GDGCHC 2 cut(s) 362, 823
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 6 cut(s) 202, 549, 809, 1190, 1273, 1311
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 6 cut(s) 161, 295, 563, 784, 899, 1291
MmeI TCCRAC 3 cut(s) 263, 796, 1387
Mox20I TGGCCA 1 cut(s) 186
MscI TGGCCA 1 cut(s) 186
MseI TTAA 1 cut(s) 1432
MslI CAYNNNNRTG 3 cut(s) 898, 1241, 1305
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 3 cut(s) 354, 518, 1418
MspI CCGG 2 cut(s) 126, 948
MspR9I CCNGG 5 cut(s) 127, 501, 686, 915, 948
MunI CAATTG 2 cut(s) 549, 1311
Mva1269I GAATGC 1 cut(s) 1354
MvaI CCWGG 3 cut(s) 501, 686, 915
MwoI GCNNNNNNNGC 7 cut(s) 521, 524, 530, 536, 539, 1257, 1427
NciI CCSGG 2 cut(s) 127, 948
NdeII GATC 4 cut(s) 427, 483, 1355, 1409
NlaIII CATG 6 cut(s) 11, 27, 460, 851, 1162, 1304
NlaIV GGNNCC 4 cut(s) 1069, 1135, 1174, 1323
NmuCI GTSAC 1 cut(s) 465
NspI RCATGY 1 cut(s) 27
NspV TTCGAA 1 cut(s) 111
OliI CACNNNNGTG 1 cut(s) 1241
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 1354
PfoI TCCNGGA 1 cut(s) 125
PleI GAGTC 6 cut(s) 161, 294, 563, 783, 898, 1290
PpsI GAGTC 6 cut(s) 161, 294, 563, 783, 898, 1290
PpuMI RGGWCCY 1 cut(s) 1322
Psp124BI GAGCTC 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 1322
Psp6I CCWGG 3 cut(s) 499, 684, 913
PspFI CCCAGC 3 cut(s) 144, 354, 438
PspGI CCWGG 3 cut(s) 499, 684, 913
PspN4I GGNNCC 4 cut(s) 1069, 1135, 1174, 1323
PspPI GGNCC 1 cut(s) 1322
PspPPI RGGWCCY 1 cut(s) 1322
PstI CTGCAG 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 1418
PvuII CAGCTG 2 cut(s) 518, 1418
RsaI GTAC 4 cut(s) 299, 875, 1042, 1174
RsaNI GTAC 4 cut(s) 298, 874, 1041, 1173
RseI CAYNNNNRTG 3 cut(s) 898, 1241, 1305
SacI GAGCTC 1 cut(s) 823
SaqAI TTAA 1 cut(s) 1432
Sau3AI GATC 4 cut(s) 427, 483, 1355, 1409
Sau96I GGNCC 1 cut(s) 1322
SchI GAGTC 6 cut(s) 161, 295, 563, 784, 899, 1291
ScrFI CCNGG 5 cut(s) 127, 501, 686, 915, 948
SduI GDGCHC 2 cut(s) 362, 823
SfaNI GCATC 3 cut(s) 73, 214, 900
SfcI CTRYAG 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 1 cut(s) 1322
SmiMI CAYNNNNRTG 3 cut(s) 898, 1241, 1305
SmlI CTYRAG 2 cut(s) 592, 869
SmoI CTYRAG 2 cut(s) 592, 869
Sse9I AATT 6 cut(s) 202, 549, 809, 1190, 1273, 1311
SsiI CCGC 5 cut(s) 352, 513, 707, 887, 1065
SspMI CTAG 1 cut(s) 833
SstI GAGCTC 1 cut(s) 823
StyD4I CCNGG 5 cut(s) 125, 499, 684, 913, 946
StyI CCWWGG 4 cut(s) 766, 837, 1005, 1129
TaaI ACNGT 2 cut(s) 565, 974
TaiI ACGT 4 cut(s) 240, 339, 467, 1076
TaqI TCGA 2 cut(s) 111, 491
TasI AATT 6 cut(s) 202, 549, 809, 1190, 1273, 1311
TatI WGTACW 2 cut(s) 873, 1040
TauI GCSGC 3 cut(s) 515, 710, 1068
Tru1I TTAA 1 cut(s) 1432
Tru9I TTAA 1 cut(s) 1432
TscAI CASTG 3 cut(s) 440, 511, 912
TseFI GTSAC 1 cut(s) 465
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 5 cut(s) 93, 358, 389, 1006, 1197
TspRI CASTG 3 cut(s) 440, 511, 912
VpaK11BI GGWCC 1 cut(s) 1322
XapI RAATTY 2 cut(s) 202, 809
XceI RCATGY 1 cut(s) 27
XspI CTAG 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.