Rh7AG158700

cellulase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010905
Physical Location & Seq
Reverse (-)
13286271 .. 13289425
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG158700.1

Sequence Viewer

Length: 1437 bp
ATGGGGCATGGTAGAGTTGTTGCATGTGTTTTGGTATTGTGGTGTTTTTTGAATGAGATAGTCTCAGCCATTGACTATGAAGATGCGCTTGGCAAATCTATATTGTTCTTCGAAGGCCAACGCTCCGGAAAGTTGCCGACCATCCAGCGAGTATCTTGGAGAGGAGACTCTGCACTTTCCGATGGCCAGCTTGAAGGAGTAAATTTGACTGGAGGATACTATGATGCAGGCGACAACGTGAAGTTCGTGTGGCCAATGGCATATAGTGTGACCTTGTTGAGTTGGAGTCTCATTGAGTACCAGCAGCAGATATCTTCAGCAAACCAGCTTGCATACGTCCAGAGAGCAATCCGCTGGGGCACAGACTTCATAATAAAAGCTCACCTTTCGCCCACCACCTTCTATACTCAGGTAGGAGATGGAAATGTGGATCACCAGTGCTGGGAGCGTCCCGAAGACATGGACTCATCACGAAAGCTCTACAAGATCACTTCGAGATCGCCAGGCAGTGAGGCCGCAGCTGAGGCTGCTGCTGCCCTCGCTGCAGCCCCAATTGTCTTTAAGACAGTTGACTCCACCTATTCGGCAAAGCTCTCAAGTCATTCCCAATCTCTGTTTGACTTGGCTGACAAGTATAGAGGGTCTTACCAAGTCTCTTGCCCTTTCTACTGCTCCTACTCTGGCTACCAGGATGAGCTGCTATGGGCTGCGGCTTGGCTATACAAGGCAAGTGGAAACAGAAGCTATTTTAGTTATGTGGTAAACAACCAAGGATGGAGTCAGGCAATGTCTGAGTTCAGTTGGGATAACAAATTTGTTGGAGCTCAGACTTTACTAGCCAAGGAATACCATGCTGGAAATACTGATTTGCTCAAGTACAAGACCCATGCGGAGTCATTTATATGTGCAGTGATGCCAGGGAGTAGCTCTACACAAGTTCAAAGAACACCCGGTGGACTTCTGTTTATTAGAGACAGTAGTAACTTGCAGTATGTTACAAGCTCTACCTTGGTGCTATTCATCTACTCAAAAACCATAAGTAGTACACAAACTGGTGGAGTTCAATGCGGCTCCACGTTTTTCTCTGCTGCGAAAATCAAAGCCTTTGCTAAAGCTCAGGTGGACTACATCCTTGGAGCCAACCCCAAGCAGATGTCATACATGGTGGGTTTTGGTACCACATACCCAACAAAATTGCACCATAGAGGTTCATCCATCCCTTCAATCAAGACCCTCCACACCAAGGTGGACTGCAATGGTGGCTATACCTATTACAATTCTGGTAGTCCAAATCCAAACACTCATGTGGGTGCAATTGTTGGGGGTCCTGATGTGAATGACCAGTTCCAGAATGCAAGATCAGACTATTCTCATTCTGAACCTACAACTTATATCAATGCAGCTTTTGTGGGATCAGCAGCTGCTTTACTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

478

Amino Acids

52.37

Weight (kDa)

7.14

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 26 - 471 1e-154 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1175
AccB1I GGYRCC 1 cut(s) 1175
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 5 cut(s) 352, 516, 710, 890, 1068
AclWI GGATC 2 cut(s) 438, 1420
AcoI YGGCCR 2 cut(s) 184, 251
AcsI RAATTY 2 cut(s) 202, 812
AcuI CTGAAG 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 953
AfaI GTAC 4 cut(s) 299, 878, 1045, 1177
AfiI CCNNNNNNNGG 2 cut(s) 442, 1243
AgsI TTSAA 5 cut(s) 52, 194, 941, 1064, 1224
AjnI CCWGG 3 cut(s) 502, 687, 916
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AleI CACNNNNGTG 2 cut(s) 1244, 1304
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 5 cut(s) 67, 159, 293, 658, 966
AlwI GGATC 2 cut(s) 438, 1420
AlwNI CAGNNNCTG 1 cut(s) 1421
Aor13HI TCCGGA 1 cut(s) 125
AoxI GGCC 4 cut(s) 115, 184, 251, 513
ApoI RAATTY 2 cut(s) 202, 812
Asp718I GGTACC 1 cut(s) 1175
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 1325
AsuC2I CCSGG 1 cut(s) 951
AsuHPI GGTGA 2 cut(s) 374, 425
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 1325
BaeGI GKGCMC 1 cut(s) 362
BalI TGGCCA 2 cut(s) 186, 253
BanI GGYRCC 1 cut(s) 1175
BanII GRGCYC 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 826
BbvCI CCTCAGC 1 cut(s) 522
BccI CCATC 5 cut(s) 149, 176, 413, 768, 1223
BciT130I CCWGG 3 cut(s) 504, 689, 918
BciVI GTATCC 1 cut(s) 209
BcnI CCSGG 1 cut(s) 951
BcoDI GTCTC 5 cut(s) 67, 159, 293, 658, 966
BfaI CTAG 1 cut(s) 836
BfmI CTRYAG 1 cut(s) 543
BfuI GTATCC 1 cut(s) 209
Bme1390I CCNGG 4 cut(s) 504, 689, 918, 951
Bme18I GGWCC 1 cut(s) 1325
BmgT120I GGNCC 1 cut(s) 1325
BmiI GGNNCC 4 cut(s) 1072, 1138, 1177, 1326
BmrFI CCNGG 4 cut(s) 504, 689, 918, 951
BmsI GCATC 3 cut(s) 73, 214, 903
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 2 cut(s) 522, 1116
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 580, 857
BpuMI CCSGG 1 cut(s) 951
BsaJI CCNNGG 6 cut(s) 769, 840, 917, 1008, 1132, 1242
BsaWI WCCGGW 1 cut(s) 125
Bsc4I CCNNNNNNNGG 2 cut(s) 442, 1243
Bse1I ACTGG 4 cut(s) 214, 436, 1057, 1342
Bse3DI GCAATG 2 cut(s) 792, 1261
BseAI TCCGGA 1 cut(s) 125
BseBI CCWGG 3 cut(s) 504, 689, 918
BseDI CCNNGG 6 cut(s) 769, 840, 917, 1008, 1132, 1242
BseGI GGATG 6 cut(s) 141, 697, 779, 1128, 1211, 1215
BseLI CCNNNNNNNGG 2 cut(s) 442, 1243
BseMI GCAATG 2 cut(s) 792, 1261
BseMII CTCAG 6 cut(s) 78, 422, 513, 783, 839, 1130
BseNI ACTGG 4 cut(s) 214, 436, 1057, 1342
BseRI GAGGAG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 362
BseYI CCCAGC 2 cut(s) 354, 441
BsgI GTGCAG 2 cut(s) 156, 927
BshFI GGCC 4 cut(s) 117, 186, 253, 515
BshNI GGYRCC 1 cut(s) 1175
BsiHKAI GWGCWC 1 cut(s) 826
BsiSI CCGG 2 cut(s) 126, 951
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 2 cut(s) 442, 1243
BsmAI GTCTC 5 cut(s) 67, 159, 293, 658, 966
BsmFI GGGAC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 1357
BsnI GGCC 4 cut(s) 117, 186, 253, 515
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 362, 826
Bsp13I TCCGGA 1 cut(s) 125
Bsp143I GATC 5 cut(s) 430, 486, 497, 1358, 1412
BspACI CCGC 5 cut(s) 352, 516, 710, 890, 1068
BspANI GGCC 4 cut(s) 117, 186, 253, 515
BspCNI CTCAG 6 cut(s) 77, 421, 514, 784, 838, 1129
BspEI TCCGGA 1 cut(s) 125
BspLI GGNNCC 4 cut(s) 1072, 1138, 1177, 1326
BspMAI CTGCAG 1 cut(s) 547
BspPI GGATC 2 cut(s) 438, 1420
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 1175
BsrDI GCAATG 2 cut(s) 792, 1261
BsrI ACTGG 4 cut(s) 214, 436, 1057, 1342
BssECI CCNNGG 6 cut(s) 769, 840, 917, 1008, 1132, 1242
BssMI GATC 5 cut(s) 430, 486, 497, 1358, 1412
BssT1I CCWWGG 5 cut(s) 769, 840, 1008, 1132, 1242
Bst2UI CCWGG 3 cut(s) 504, 689, 918
Bst4CI ACNGT 2 cut(s) 568, 977
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 188, 229, 330
BstDEI CTNAG 6 cut(s) 64, 408, 522, 792, 825, 1116
BstF5I GGATG 6 cut(s) 141, 697, 779, 1128, 1211, 1215
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 5 cut(s) 433, 489, 500, 1361, 1415
BstMAI GTCTC 5 cut(s) 67, 159, 293, 658, 966
BstMBI GATC 5 cut(s) 430, 486, 497, 1358, 1412
BstMWI GCNNNNNNNGC 7 cut(s) 524, 527, 533, 539, 542, 1260, 1430
BstNI CCWGG 3 cut(s) 504, 689, 918
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 4 cut(s) 502, 687, 916, 949
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 462
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 4 cut(s) 117, 186, 253, 515
BtsCI GGATG 6 cut(s) 141, 697, 779, 1128, 1211, 1215
BtsI GCAGTG 2 cut(s) 514, 915
BtsIMutI CAGTG 3 cut(s) 443, 514, 915
Cac8I GCNNGC 3 cut(s) 188, 229, 330
CaiI CAGNNNCTG 1 cut(s) 1421
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 1325
CseI GACGC 1 cut(s) 437
Csp6I GTAC 4 cut(s) 298, 877, 1044, 1176
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviAII CATG 7 cut(s) 8, 24, 460, 851, 887, 1162, 1304
CviQI GTAC 4 cut(s) 298, 877, 1044, 1176
DdeI CTNAG 6 cut(s) 64, 408, 522, 792, 825, 1116
DpnI GATC 5 cut(s) 432, 488, 499, 1360, 1414
DpnII GATC 5 cut(s) 430, 486, 497, 1358, 1412
DraIII CACNNNGTG 1 cut(s) 953
EaeI YGGCCR 2 cut(s) 184, 251
Ecl136II GAGCTC 1 cut(s) 824
Eco130I CCWWGG 5 cut(s) 769, 840, 1008, 1132, 1242
Eco24I GRGCYC 1 cut(s) 826
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 1325
Eco53kI GAGCTC 1 cut(s) 824
Eco57I CTGAAG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 1325
EcoRII CCWGG 3 cut(s) 502, 687, 916
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 5 cut(s) 769, 840, 1008, 1132, 1242
EcoT38I GRGCYC 1 cut(s) 826
ErhI CCWWGG 5 cut(s) 769, 840, 1008, 1132, 1242
FaeI CATG 7 cut(s) 11, 27, 463, 854, 890, 1165, 1307
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 369, 401
FaqI GGGAC 1 cut(s) 435
FatI CATG 7 cut(s) 7, 23, 459, 850, 886, 1161, 1303
FokI GGATG 6 cut(s) 128, 704, 786, 1115, 1198, 1202
FriOI GRGCYC 1 cut(s) 826
FspBI CTAG 1 cut(s) 836
GlaI GCGC 1 cut(s) 87
GsaI CCCAGC 2 cut(s) 358, 445
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 4 cut(s) 117, 186, 253, 515
HapII CCGG 2 cut(s) 126, 951
HgaI GACGC 1 cut(s) 437
HhaI GCGC 1 cut(s) 88
Hin1II CATG 7 cut(s) 11, 27, 463, 854, 890, 1165, 1307
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 6 cut(s) 167, 286, 464, 572, 778, 893
HpaII CCGG 2 cut(s) 126, 951
HphI GGTGA 2 cut(s) 374, 425
Hpy166II GTNNAC 6 cut(s) 571, 763, 956, 1046, 1123, 1249
Hpy188I TCNGA 5 cut(s) 181, 793, 828, 1363, 1378
Hpy188III TCNNGA 8 cut(s) 126, 340, 452, 471, 495, 1228, 1328, 1348
Hpy8I GTNNAC 6 cut(s) 571, 763, 956, 1046, 1123, 1249
HpyAV CCTTC 4 cut(s) 107, 188, 409, 1230
HpyCH4III ACNGT 2 cut(s) 568, 977
HpyCH4IV ACGT 3 cut(s) 237, 336, 1076
HpyF10VI GCNNNNNNNGC 7 cut(s) 524, 527, 533, 539, 542, 1260, 1430
HpyF3I CTNAG 6 cut(s) 64, 408, 522, 792, 825, 1116
HpySE526I ACGT 3 cut(s) 237, 336, 1076
Hsp92II CATG 7 cut(s) 11, 27, 463, 854, 890, 1165, 1307
HspAI GCGC 1 cut(s) 86
Kpn2I TCCGGA 1 cut(s) 125
KpnI GGTACC 1 cut(s) 1179
Kzo9I GATC 5 cut(s) 430, 486, 497, 1358, 1412
LmnI GCTCC 6 cut(s) 128, 445, 677, 821, 1076, 1136
LweI GCATC 3 cut(s) 73, 214, 903
MaeI CTAG 1 cut(s) 836
MaeII ACGT 3 cut(s) 237, 336, 1076
MaeIII GTNAC 3 cut(s) 268, 980, 994
MalI GATC 5 cut(s) 432, 488, 499, 1360, 1414
MboI GATC 5 cut(s) 430, 486, 497, 1358, 1412
MboII GAAGA 4 cut(s) 92, 100, 306, 467
MfeI CAATTG 2 cut(s) 552, 1314
MhlI GDGCHC 2 cut(s) 362, 826
MlsI TGGCCA 2 cut(s) 186, 253
MluCI AATT 6 cut(s) 202, 552, 812, 1193, 1276, 1314
MluNI TGGCCA 2 cut(s) 186, 253
MlyI GAGTC 6 cut(s) 161, 295, 458, 566, 787, 902
MmeI TCCRAC 2 cut(s) 263, 799
MnlI CCTC 8 cut(s) 155, 206, 505, 517, 548, 632, 1199, 1244
Mox20I TGGCCA 2 cut(s) 186, 253
MroI TCCGGA 1 cut(s) 125
MscI TGGCCA 2 cut(s) 186, 253
MseI TTAA 2 cut(s) 561, 1435
MslI CAYNNNNRTG 4 cut(s) 901, 1244, 1304, 1308
Msp20I TGGCCA 2 cut(s) 186, 253
MspA1I CMGCKG 3 cut(s) 354, 521, 1421
MspI CCGG 2 cut(s) 126, 951
MspR9I CCNGG 4 cut(s) 504, 689, 918, 951
MunI CAATTG 2 cut(s) 552, 1314
Mva1269I GAATGC 1 cut(s) 1357
MvaI CCWGG 3 cut(s) 504, 689, 918
MwoI GCNNNNNNNGC 7 cut(s) 524, 527, 533, 539, 542, 1260, 1430
NciI CCSGG 1 cut(s) 951
NdeII GATC 5 cut(s) 430, 486, 497, 1358, 1412
NlaIII CATG 7 cut(s) 11, 27, 463, 854, 890, 1165, 1307
NlaIV GGNNCC 4 cut(s) 1072, 1138, 1177, 1326
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 27
NspV TTCGAA 1 cut(s) 111
OliI CACNNNNGTG 2 cut(s) 1244, 1304
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 1357
PleI GAGTC 6 cut(s) 161, 294, 458, 566, 786, 901
PpsI GAGTC 6 cut(s) 161, 294, 458, 566, 786, 901
PpuMI RGGWCCY 1 cut(s) 1325
Psp124BI GAGCTC 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 1325
Psp6I CCWGG 3 cut(s) 502, 687, 916
PspFI CCCAGC 2 cut(s) 354, 441
PspGI CCWGG 3 cut(s) 502, 687, 916
PspN4I GGNNCC 4 cut(s) 1072, 1138, 1177, 1326
PspPI GGNCC 1 cut(s) 1325
PspPPI RGGWCCY 1 cut(s) 1325
PstI CTGCAG 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 1421
PvuII CAGCTG 2 cut(s) 521, 1421
RsaI GTAC 4 cut(s) 299, 878, 1045, 1177
RsaNI GTAC 4 cut(s) 298, 877, 1044, 1176
RseI CAYNNNNRTG 4 cut(s) 901, 1244, 1304, 1308
SacI GAGCTC 1 cut(s) 826
SaqAI TTAA 2 cut(s) 561, 1435
Sau3AI GATC 5 cut(s) 430, 486, 497, 1358, 1412
Sau96I GGNCC 1 cut(s) 1325
SchI GAGTC 6 cut(s) 161, 295, 458, 566, 787, 902
ScrFI CCNGG 4 cut(s) 504, 689, 918, 951
SduI GDGCHC 2 cut(s) 362, 826
SfaNI GCATC 3 cut(s) 73, 214, 903
SfcI CTRYAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 1 cut(s) 1325
SmiMI CAYNNNNRTG 4 cut(s) 901, 1244, 1304, 1308
SmlI CTYRAG 2 cut(s) 595, 872
SmoI CTYRAG 2 cut(s) 595, 872
Sse9I AATT 6 cut(s) 202, 552, 812, 1193, 1276, 1314
SsiI CCGC 5 cut(s) 352, 516, 710, 890, 1068
SspMI CTAG 1 cut(s) 836
SstI GAGCTC 1 cut(s) 826
StyD4I CCNGG 4 cut(s) 502, 687, 916, 949
StyI CCWWGG 5 cut(s) 769, 840, 1008, 1132, 1242
TaaI ACNGT 2 cut(s) 568, 977
TaiI ACGT 3 cut(s) 240, 339, 1079
TaqI TCGA 2 cut(s) 111, 494
TasI AATT 6 cut(s) 202, 552, 812, 1193, 1276, 1314
TatI WGTACW 2 cut(s) 876, 1043
TauI GCSGC 3 cut(s) 518, 713, 1071
Tru1I TTAA 2 cut(s) 561, 1435
Tru9I TTAA 2 cut(s) 561, 1435
TscAI CASTG 3 cut(s) 443, 514, 915
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 4 cut(s) 93, 358, 1009, 1200
TspRI CASTG 3 cut(s) 443, 514, 915
VpaK11BI GGWCC 1 cut(s) 1325
XapI RAATTY 2 cut(s) 202, 812
XceI RCATGY 1 cut(s) 27
XspI CTAG 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.