FvH4_5g03220

cellulase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010905
Physical Location & Seq
Forward (+)
1912259 .. 1914883
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03220.t1

Sequence Viewer

Length: 1446 bp
ATGATGCGGCATGGTAGAGTTGTTGCATGTGTTTTGGTGTTGTGGTGTTGGTGTGTTTTGAATGGGGTAGTCTCGGCCATTGACTATAAAGATGCGCTAGGCAAATCTATATTGTTCTTCGAAGGCCAACGCTCCGGGAAGTTGCCAAACTCCCAGCGAGTAGTTTGGAGAGGAGACTCTGCACTTTCCGATGGCCAGCTCGAAGGAGTGAATCTGACCGGAGGATACTACGACGCAGGCGACAACGTGAAGTTCGGGTGGCCAATGGCATATAGTGTGAGCTTGTTGAGTTGGAGTCTCATTGAGTACCAGCAGCAGATATCTTCAGCAAAGCAGCTTGCATATCTCCAAGGAGCAATCCGCTGGGGCACAGAATTCATAATAAAAGCTCACCCTTCGCCTACCACATTCTATACTCAGGTAGGAGATGGAAATGCGGATCACCAGTGCTGGGAGCGTCCGGAAGACATGGACACATCAAGGAAGCTCTACAAGATCACTTCGAGTTCTCCAGGCAGTGAGGCCGCAGCTGAGGCTGCTGCTGCCCTCGCTGCAGCCTCGATTGTCTTTAAAACAGTTGACTCCACCTATTCTGCGAAGCTCTTACGTTATTCCAAATCTCTGTTTGACTTGGCTGACAAGTATAGAGGGTCTTACCAAGTCTCTTGCCCTTTCTACTGCTCCTACTCTGGCTACCAGGATGAGCTGTTATGGGCTGCGGCTTGGCTATACAAGGCAAGTGGAAACAGAAGCTATTTGAGTTATGTGCTAAACAACCAAGGATGGAGTCAGGCTATGTCCGAGTTCAGTTGGGATAACAAATTTGTTGGAGCTCAGACTATACTAGCCAAAGAATACCATGCCGGAAACACGAATTTACTCAAGTACAAGACCGATGCGGAGTCATTTATATGTGCAGTGATGCCAGGGAGTAGCTCTTCACAAGTTCAAAGAACACCTGGTGGACTTCTGTTCATTAGAGATAGTAGTAACTTGCAGTATGTTACAAGCTCTACCTTGGTGCTCTTCATCTACTCAAAAACAATAAGTAGTACACAAACTCGTGGAGTTCAATGCGGCTCCATGTTTTTCTCTGCTGAGAAAATCAAAGCCTTTGCTAAATCTCAGGTGGACTACATCCTTGGAGCCAACCCCAAGAAGATGTCATACATGGTAGGTTTTGGTACCACATACCCAACAAAAGTGCACCATAGAGGTTCATCCATCCCTTCAATCAAGAAAGTCCGCACCAAGGTGGACTGCAATGGTGGCTATACCTATTACAATTCTGGGAGTCCGAATCCAAATACTCATGTGGGTGCAATTGTTGGGGGTCCTGATGTGAATGACCAGTTCCAGAATGCAAGATCAGACTATTCTCATTCTGAACCTACAACTTATATCAATGCAGCTTTTGTGGGATCAGCAGCTGCTTTACTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

482

Amino Acids

52.81

Weight (kDa)

8.81

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 29 - 474 1e-157 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1184
AccB1I GGYRCC 1 cut(s) 1184
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 8 cut(s) 7, 361, 437, 525, 719, 899, 1077, 1246
AclWI GGATC 2 cut(s) 447, 1429
AcoI YGGCCR 3 cut(s) 75, 193, 260
AcsI RAATTY 3 cut(s) 374, 821, 874
AcuI CTGAAG 1 cut(s) 309
AdeI CACNNNGTG 1 cut(s) 962
AfaI GTAC 4 cut(s) 308, 887, 1054, 1186
AfiI CCNNNNNNNGG 2 cut(s) 451, 1252
AgsI TTSAA 4 cut(s) 61, 950, 1073, 1233
AjnI CCWGG 4 cut(s) 511, 696, 925, 958
AleI CACNNNNGTG 1 cut(s) 1253
Alw21I GWGCWC 3 cut(s) 835, 1026, 1209
Alw26I GTCTC 4 cut(s) 76, 168, 302, 667
Alw44I GTGCAC 1 cut(s) 1205
AlwI GGATC 2 cut(s) 447, 1429
AlwNI CAGNNNCTG 1 cut(s) 1430
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 5 cut(s) 75, 124, 193, 260, 522
ApaLI GTGCAC 1 cut(s) 1205
ApoI RAATTY 3 cut(s) 374, 821, 874
Asp718I GGTACC 1 cut(s) 1184
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 1334
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 383, 434
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 1334
BaeGI GKGCMC 2 cut(s) 371, 1209
BalI TGGCCA 2 cut(s) 195, 262
BanI GGYRCC 1 cut(s) 1184
BanII GRGCYC 1 cut(s) 835
BarI GAAGNNNNNNTAC 2 cut(s) 1151, 1183
BauI CACGAG 1 cut(s) 1062
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 3 cut(s) 835, 1026, 1209
BbvCI CCTCAGC 1 cut(s) 531
BccI CCATC 4 cut(s) 185, 422, 777, 1232
BciT130I CCWGG 4 cut(s) 513, 698, 927, 960
BciVI GTATCC 1 cut(s) 218
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 4 cut(s) 76, 168, 302, 667
BfaI CTAG 3 cut(s) 98, 845, 1439
BfmI CTRYAG 1 cut(s) 552
BfuI GTATCC 1 cut(s) 218
Bme1390I CCNGG 5 cut(s) 136, 513, 698, 927, 960
Bme18I GGWCC 1 cut(s) 1334
BmgT120I GGNCC 1 cut(s) 1334
BmiI GGNNCC 4 cut(s) 1081, 1147, 1186, 1335
BmrFI CCNGG 5 cut(s) 136, 513, 698, 927, 960
BmsI GCATC 3 cut(s) 82, 886, 912
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 531
Bpu14I TTCGAA 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 136
BsaJI CCNNGG 6 cut(s) 349, 778, 926, 1017, 1141, 1251
BsaWI WCCGGW 2 cut(s) 218, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 1252
Bse1I ACTGG 2 cut(s) 445, 1351
Bse3DI GCAATG 1 cut(s) 1270
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 4 cut(s) 513, 698, 927, 960
BseDI CCNNGG 6 cut(s) 349, 778, 926, 1017, 1141, 1251
BseGI GGATG 5 cut(s) 706, 788, 1137, 1220, 1224
BseLI CCNNNNNNNGG 2 cut(s) 451, 1252
BseMI GCAATG 1 cut(s) 1270
BseMII CTCAG 5 cut(s) 431, 522, 848, 1089, 1139
BseNI ACTGG 2 cut(s) 445, 1351
BseRI GAGGAG 1 cut(s) 186
BseSI GKGCMC 2 cut(s) 371, 1209
BseYI CCCAGC 3 cut(s) 153, 363, 450
BsgI GTGCAG 2 cut(s) 165, 936
BshFI GGCC 5 cut(s) 77, 126, 195, 262, 524
BshNI GGYRCC 1 cut(s) 1184
BsiHKAI GWGCWC 3 cut(s) 835, 1026, 1209
BsiSI CCGG 4 cut(s) 135, 219, 461, 864
BslI CCNNNNNNNGG 2 cut(s) 451, 1252
BsmAI GTCTC 4 cut(s) 76, 168, 302, 667
BsmI GAATGC 1 cut(s) 1366
BsnI GGCC 5 cut(s) 77, 126, 195, 262, 524
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 4 cut(s) 371, 835, 1026, 1209
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 4 cut(s) 439, 495, 1367, 1421
BspACI CCGC 8 cut(s) 7, 361, 437, 525, 719, 899, 1077, 1246
BspANI GGCC 5 cut(s) 77, 126, 195, 262, 524
BspCNI CTCAG 5 cut(s) 430, 523, 847, 1090, 1138
BspEI TCCGGA 1 cut(s) 460
BspLI GGNNCC 4 cut(s) 1081, 1147, 1186, 1335
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 2 cut(s) 447, 1429
BspQI GCTCTTC 2 cut(s) 943, 1031
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 1184
BsrDI GCAATG 1 cut(s) 1270
BsrI ACTGG 2 cut(s) 445, 1351
BssECI CCNNGG 6 cut(s) 349, 778, 926, 1017, 1141, 1251
BssMI GATC 4 cut(s) 439, 495, 1367, 1421
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 5 cut(s) 349, 778, 1017, 1141, 1251
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 4 cut(s) 513, 698, 927, 960
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 2 cut(s) 943, 1031
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 197, 238, 339
BstDEI CTNAG 5 cut(s) 417, 531, 834, 1098, 1125
BstF5I GGATG 5 cut(s) 706, 788, 1137, 1220, 1224
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 4 cut(s) 442, 498, 1370, 1424
BstMAI GTCTC 4 cut(s) 76, 168, 302, 667
BstMBI GATC 4 cut(s) 439, 495, 1367, 1421
BstMWI GCNNNNNNNGC 7 cut(s) 533, 536, 542, 548, 551, 1269, 1439
BstNI CCWGG 4 cut(s) 513, 698, 927, 960
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 5 cut(s) 134, 511, 696, 925, 958
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 2 cut(s) 371, 1209
BstV2I GAAGAC 1 cut(s) 471
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 5 cut(s) 77, 126, 195, 262, 524
BtsCI GGATG 5 cut(s) 706, 788, 1137, 1220, 1224
BtsI GCAGTG 2 cut(s) 523, 924
BtsIMutI CAGTG 3 cut(s) 452, 523, 924
Cac8I GCNNGC 3 cut(s) 197, 238, 339
CaiI CAGNNNCTG 1 cut(s) 1430
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 1334
CseI GACGC 2 cut(s) 242, 446
CsiI ACCWGGT 1 cut(s) 958
Csp6I GTAC 4 cut(s) 307, 886, 1053, 1185
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviAII CATG 7 cut(s) 11, 27, 469, 860, 1084, 1171, 1313
CviQI GTAC 4 cut(s) 307, 886, 1053, 1185
DdeI CTNAG 5 cut(s) 417, 531, 834, 1098, 1125
DpnI GATC 4 cut(s) 441, 497, 1369, 1423
DpnII GATC 4 cut(s) 439, 495, 1367, 1421
DraI TTTAAA 1 cut(s) 571
DraIII CACNNNGTG 1 cut(s) 962
EaeI YGGCCR 3 cut(s) 75, 193, 260
Eam1104I CTCTTC 2 cut(s) 943, 1031
EarI CTCTTC 2 cut(s) 943, 1031
Ecl136II GAGCTC 1 cut(s) 833
Eco130I CCWWGG 5 cut(s) 349, 778, 1017, 1141, 1251
Eco24I GRGCYC 1 cut(s) 835
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 1334
Eco53kI GAGCTC 1 cut(s) 833
Eco57I CTGAAG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 1334
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 4 cut(s) 511, 696, 925, 958
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 5 cut(s) 349, 778, 1017, 1141, 1251
EcoT38I GRGCYC 1 cut(s) 835
ErhI CCWWGG 5 cut(s) 349, 778, 1017, 1141, 1251
FaeI CATG 7 cut(s) 14, 30, 472, 863, 1087, 1174, 1316
FatI CATG 7 cut(s) 10, 26, 468, 859, 1083, 1170, 1312
FokI GGATG 5 cut(s) 713, 795, 1124, 1207, 1211
FriOI GRGCYC 1 cut(s) 835
FspBI CTAG 3 cut(s) 98, 845, 1439
GlaI GCGC 1 cut(s) 96
GsaI CCCAGC 3 cut(s) 157, 367, 454
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 5 cut(s) 77, 126, 195, 262, 524
HapII CCGG 4 cut(s) 135, 219, 461, 864
HgaI GACGC 2 cut(s) 242, 446
HhaI GCGC 1 cut(s) 97
Hin1II CATG 7 cut(s) 14, 30, 472, 863, 1087, 1174, 1316
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 8 cut(s) 176, 211, 295, 581, 787, 902, 1294, 1300
HpaII CCGG 4 cut(s) 135, 219, 461, 864
HphI GGTGA 2 cut(s) 383, 434
Hpy166II GTNNAC 6 cut(s) 580, 965, 1055, 1132, 1207, 1258
Hpy188I TCNGA 7 cut(s) 190, 216, 802, 837, 1299, 1372, 1387
Hpy188III TCNNGA 4 cut(s) 461, 1237, 1337, 1357
Hpy8I GTNNAC 6 cut(s) 580, 965, 1055, 1132, 1207, 1258
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 4 cut(s) 116, 197, 405, 1239
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 2 cut(s) 246, 607
HpyF10VI GCNNNNNNNGC 7 cut(s) 533, 536, 542, 548, 551, 1269, 1439
HpyF3I CTNAG 5 cut(s) 417, 531, 834, 1098, 1125
HpySE526I ACGT 2 cut(s) 246, 607
Hsp92II CATG 7 cut(s) 14, 30, 472, 863, 1087, 1174, 1316
HspAI GCGC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 460
KpnI GGTACC 1 cut(s) 1188
Kzo9I GATC 4 cut(s) 439, 495, 1367, 1421
LguI GCTCTTC 2 cut(s) 943, 1031
LmnI GCTCC 7 cut(s) 137, 353, 454, 686, 830, 1085, 1145
LweI GCATC 3 cut(s) 82, 886, 912
MabI ACCWGGT 1 cut(s) 958
MaeI CTAG 3 cut(s) 98, 845, 1439
MaeII ACGT 2 cut(s) 246, 607
MaeIII GTNAC 2 cut(s) 989, 1003
MalI GATC 4 cut(s) 441, 497, 1369, 1423
MboI GATC 4 cut(s) 439, 495, 1367, 1421
MboII GAAGA 6 cut(s) 109, 315, 476, 930, 1018, 1171
MfeI CAATTG 1 cut(s) 1323
MhlI GDGCHC 4 cut(s) 371, 835, 1026, 1209
MlsI TGGCCA 2 cut(s) 195, 262
MluCI AATT 5 cut(s) 374, 821, 874, 1285, 1323
MluNI TGGCCA 2 cut(s) 195, 262
MlyI GAGTC 6 cut(s) 170, 304, 575, 796, 911, 1303
MmeI TCCRAC 2 cut(s) 272, 808
MnlI CCTC 8 cut(s) 164, 215, 514, 526, 557, 568, 641, 1208
Mox20I TGGCCA 2 cut(s) 195, 262
MroI TCCGGA 1 cut(s) 460
MscI TGGCCA 2 cut(s) 195, 262
MseI TTAA 2 cut(s) 570, 1444
MslI CAYNNNNRTG 3 cut(s) 910, 1253, 1317
Msp20I TGGCCA 2 cut(s) 195, 262
MspA1I CMGCKG 3 cut(s) 363, 530, 1430
MspI CCGG 4 cut(s) 135, 219, 461, 864
MspR9I CCNGG 5 cut(s) 136, 513, 698, 927, 960
MunI CAATTG 1 cut(s) 1323
Mva1269I GAATGC 1 cut(s) 1366
MvaI CCWGG 4 cut(s) 513, 698, 927, 960
MwoI GCNNNNNNNGC 7 cut(s) 533, 536, 542, 548, 551, 1269, 1439
NciI CCSGG 1 cut(s) 136
NdeII GATC 4 cut(s) 439, 495, 1367, 1421
NlaIII CATG 7 cut(s) 14, 30, 472, 863, 1087, 1174, 1316
NlaIV GGNNCC 4 cut(s) 1081, 1147, 1186, 1335
NmeAIII GCCGAG 1 cut(s) 53
NspI RCATGY 1 cut(s) 30
NspV TTCGAA 1 cut(s) 120
OliI CACNNNNGTG 1 cut(s) 1253
PciSI GCTCTTC 2 cut(s) 943, 1031
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 1366
PfeI GAWTC 2 cut(s) 211, 1300
PfoI TCCNGGA 1 cut(s) 134
PleI GAGTC 6 cut(s) 170, 303, 575, 795, 910, 1302
PpsI GAGTC 6 cut(s) 170, 303, 575, 795, 910, 1302
PpuMI RGGWCCY 1 cut(s) 1334
Psp124BI GAGCTC 1 cut(s) 835
Psp5II RGGWCCY 1 cut(s) 1334
Psp6I CCWGG 4 cut(s) 511, 696, 925, 958
PspFI CCCAGC 3 cut(s) 153, 363, 450
PspGI CCWGG 4 cut(s) 511, 696, 925, 958
PspN4I GGNNCC 4 cut(s) 1081, 1147, 1186, 1335
PspPI GGNCC 1 cut(s) 1334
PspPPI RGGWCCY 1 cut(s) 1334
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 1430
PvuII CAGCTG 2 cut(s) 530, 1430
RsaI GTAC 4 cut(s) 308, 887, 1054, 1186
RsaNI GTAC 4 cut(s) 307, 886, 1053, 1185
RseI CAYNNNNRTG 3 cut(s) 910, 1253, 1317
SacI GAGCTC 1 cut(s) 835
SapI GCTCTTC 2 cut(s) 943, 1031
SaqAI TTAA 2 cut(s) 570, 1444
Sau3AI GATC 4 cut(s) 439, 495, 1367, 1421
Sau96I GGNCC 1 cut(s) 1334
SchI GAGTC 6 cut(s) 170, 304, 575, 796, 911, 1303
ScrFI CCNGG 5 cut(s) 136, 513, 698, 927, 960
SduI GDGCHC 4 cut(s) 371, 835, 1026, 1209
SexAI ACCWGGT 1 cut(s) 958
SfaNI GCATC 3 cut(s) 82, 886, 912
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 1334
SmiMI CAYNNNNRTG 3 cut(s) 910, 1253, 1317
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
Sse9I AATT 5 cut(s) 374, 821, 874, 1285, 1323
SsiI CCGC 8 cut(s) 7, 361, 437, 525, 719, 899, 1077, 1246
SspMI CTAG 3 cut(s) 98, 845, 1439
SstI GAGCTC 1 cut(s) 835
StyD4I CCNGG 5 cut(s) 134, 511, 696, 925, 958
StyI CCWWGG 5 cut(s) 349, 778, 1017, 1141, 1251
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 2 cut(s) 249, 610
TaqI TCGA 4 cut(s) 120, 201, 503, 560
TaqII GACCGA 1 cut(s) 908
TasI AATT 5 cut(s) 374, 821, 874, 1285, 1323
TatI WGTACW 2 cut(s) 885, 1052
TauI GCSGC 4 cut(s) 10, 527, 722, 1080
TfiI GAWTC 2 cut(s) 211, 1300
Tru1I TTAA 2 cut(s) 570, 1444
Tru9I TTAA 2 cut(s) 570, 1444
TscAI CASTG 3 cut(s) 452, 523, 924
TspDTI ATGAA 4 cut(s) 367, 964, 1018, 1209
TspRI CASTG 3 cut(s) 452, 523, 924
VneI GTGCAC 1 cut(s) 1205
VpaK11BI GGWCC 1 cut(s) 1334
XapI RAATTY 3 cut(s) 374, 821, 874
XceI RCATGY 1 cut(s) 30
XspI CTAG 3 cut(s) 98, 845, 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.