AT4G23560

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010905
Physical Location & Seq
Reverse (-)
12293117 .. 12295859
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23560.1

Sequence Viewer

Length: 1440 bp
ATGGGAAAGCTCTTGGTGTTAATGTTAGTTGGGATGTTCTTGGCTTTTGAAAGCTTGGAAGCTCTTGAATATGGAGATGCACTTAACAAGTCAATCTTGTTCTTTGAAGGCCAACGATCCGGTAAACTCCCGACTAACCAACGAGTTAAATGGCGAGCCGATTCTGCCCTCTCTGATGGTTCACTTGCTAATGTGAATTTAATTGGAGGATATTACGATGCTGGTGACAATGTAAAATTTGTGTGGCCAATGTCATTTACCACAACCTTGTTAAGTTGGGCTGCAATTGAATACCAAAATGAGATTTCTTCCGTTAACCAGCTCGGTTATCTCCGGTCTACTATCAAATGGGGAACCGATTTCATCCTACGCGCTCATACATCTCCCAACATGCTATATACCCAGGTGGGAGATGGTAATTCAGATCATAGTTGTTGGGAGAGGCCAGAAGATATGGACACATCAAGAACTCTCTACAGTATCTCTTCCTCTTCCCCTGGTTCTGAAGCCGCGGGTGAAGCCGCAGCCGCTCTTGCAGCGGCCTCACTTGTTTTCAAATCGGTCGATTCTACTTATTCATCCACACTATTAAACCATGCAAAAACCCTATTTGAATTTGCTGATAAGTACAGAGGTTCTTATCAAGCATCTTGCCCTTTCTACTGCTCCTACTCCGGCTACCAGGATGAATTATTATGGGCTGCGGCTTGGCTATATAAAGCAACAGGAGATAAAATTTACATAAATTATGTTATAAGCAATAAAGATTGGAGCCAAGCCGTTAATGAATTCAGTTGGGACAACAAATTCGTCGGCGCTCAAGCTTTACTCGTATCGGAGTTTTACAACGGGGCAAATGACTTGGCAAAATTTAAGTCCGATGTTGAATCCTTCGTCTGTGCAATGATGCCAGGAAGTAGCTCTCAACAAATTAAACCAACTCCTGGTGGCCTTTTGTTTATTAGAGACAGTAGCAACTTACAATATGTAACAACGGCTACAACTGTTTTGTTCCATTACTCAAAAACTCTTACCAAAGCCGGGGTTGGTTCAATCCAGTGCGGTTCGACCAAGTTCACCGTATCTCAAATTCGAAATTTTGCCAAATCACAGGTTGATTATATCCTTGGAAATAATCCAATGAAAATGTCTTACATGGTCGGATTTGGGACAAAGTATCCAACACAACCTCATCATAGAGGCTCATCTTTACCATCGATCCAATCTAAACCGGAGAAAATTGACTGTAATGGAGGATATTCATACTACAATTCTGATACACCAAACCCGAATGTGCATATTGGTGCAATTGTGGGCGGACCGAATTCATCCGATCAGTATAGCGACAAAAAATCAGATTACTCTCATGCTGAGCCCACAACTTACATCAACGCCGCCTTTATTGGACCTGTTGCAGCTCTAATCAGTTCTTCCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

479

Amino Acids

52.45

Weight (kDa)

5.93

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 24 - 469 1.3e-161 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccB7I CCANNNNNTGG 1 cut(s) 944
AccBSI CCGCTC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 338
AccII CGCG 2 cut(s) 372, 512
AccIII TCCGGA 1 cut(s) 1433
AciI CCGC 9 cut(s) 510, 512, 522, 528, 539, 704, 1062, 1317, 1395
AclWI GGATC 2 cut(s) 111, 1213
AcoI YGGCCR 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 2 cut(s) 944, 1041
AgsI TTSAA 8 cut(s) 50, 68, 107, 290, 556, 614, 887, 1053
AjnI CCWGG 5 cut(s) 402, 496, 681, 910, 943
AluBI AGCT 7 cut(s) 10, 54, 62, 322, 824, 921, 1418
AluI AGCT 7 cut(s) 10, 54, 62, 322, 824, 921, 1418
Alw26I GTCTC 1 cut(s) 960
AlwI GGATC 2 cut(s) 111, 1213
Aor13HI TCCGGA 1 cut(s) 1433
AoxI GGCC 5 cut(s) 109, 245, 443, 540, 949
ApeKI GCWGC 5 cut(s) 281, 524, 536, 701, 1415
ArsI GACNNNNNNTTYG 4 cut(s) 791, 823, 860, 892
AspLEI GCGC 2 cut(s) 374, 818
AspS9I GGNCC 2 cut(s) 1319, 1406
AsuC2I CCSGG 1 cut(s) 1042
AsuHPI GGTGA 3 cut(s) 236, 527, 1069
AsuII TTCGAA 1 cut(s) 1093
AvaII GGWCC 2 cut(s) 1319, 1406
BalI TGGCCA 1 cut(s) 247
BanII GRGCYC 1 cut(s) 1377
BbvI GCAGC 5 cut(s) 268, 536, 548, 688, 1427
BccI CCATC 3 cut(s) 170, 407, 1222
BceAI ACGGC 2 cut(s) 764, 1011
BciT130I CCWGG 5 cut(s) 404, 498, 683, 912, 945
BciVI GTATCC 1 cut(s) 1188
BcnI CCSGG 1 cut(s) 1042
BcoDI GTCTC 1 cut(s) 960
BfmI CTRYAG 1 cut(s) 475
BfoI RGCGCY 1 cut(s) 819
BfuI GTATCC 1 cut(s) 1188
BlpI GCTNAGC 1 cut(s) 1371
Bme1390I CCNGG 6 cut(s) 404, 498, 683, 912, 945, 1042
Bme18I GGWCC 2 cut(s) 1319, 1406
BmgT120I GGNCC 2 cut(s) 1319, 1406
BmiI GGNNCC 2 cut(s) 355, 773
BmrFI CCNGG 6 cut(s) 404, 498, 683, 912, 945, 1042
BmsI GCATC 4 cut(s) 67, 208, 656, 897
Bpu1102I GCTNAGC 1 cut(s) 1371
Bpu14I TTCGAA 1 cut(s) 1093
BpuEI CTTGAG 1 cut(s) 804
BpuMI CCSGG 1 cut(s) 1042
Bsa29I ATCGAT 1 cut(s) 1217
BsaJI CCNNGG 5 cut(s) 402, 496, 510, 1041, 1126
BsaWI WCCGGW 4 cut(s) 119, 333, 1231, 1433
Bsc4I CCNNNNNNNGG 2 cut(s) 944, 1041
Bse1I ACTGG 1 cut(s) 1057
Bse3DI GCAATG 1 cut(s) 909
BseAI TCCGGA 1 cut(s) 1433
BseBI CCWGG 5 cut(s) 404, 498, 683, 912, 945
BseCI ATCGAT 1 cut(s) 1217
BseDI CCNNGG 5 cut(s) 402, 496, 510, 1041, 1126
BseGI GGATG 5 cut(s) 39, 363, 578, 691, 1328
BseLI CCNNNNNNNGG 2 cut(s) 944, 1041
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 1 cut(s) 1362
BseNI ACTGG 1 cut(s) 1057
BseXI GCAGC 5 cut(s) 268, 536, 548, 688, 1427
Bsh1236I CGCG 2 cut(s) 372, 512
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 5 cut(s) 111, 247, 445, 542, 951
BshVI ATCGAT 1 cut(s) 1217
BsiEI CGRYCG 1 cut(s) 564
BsiSI CCGG 6 cut(s) 120, 334, 675, 1041, 1232, 1434
BslFI GGGAC 2 cut(s) 812, 1183
BslI CCNNNNNNNGG 2 cut(s) 944, 1041
BsmAI GTCTC 1 cut(s) 960
BsmFI GGGAC 2 cut(s) 812, 1183
BsnI GGCC 5 cut(s) 111, 247, 445, 542, 951
Bsp119I TTCGAA 1 cut(s) 1093
Bsp1286I GDGCHC 1 cut(s) 1377
Bsp13I TCCGGA 1 cut(s) 1433
Bsp143I GATC 4 cut(s) 116, 424, 1218, 1333
Bsp1720I GCTNAGC 1 cut(s) 1371
BspACI CCGC 9 cut(s) 510, 512, 522, 528, 539, 704, 1062, 1317, 1395
BspANI GGCC 5 cut(s) 111, 247, 445, 542, 951
BspCNI CTCAG 1 cut(s) 1363
BspDI ATCGAT 1 cut(s) 1217
BspEI TCCGGA 1 cut(s) 1433
BspFNI CGCG 2 cut(s) 372, 512
BspLI GGNNCC 2 cut(s) 355, 773
BspPI GGATC 2 cut(s) 111, 1213
BspT104I TTCGAA 1 cut(s) 1093
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 1 cut(s) 1057
BssECI CCNNGG 5 cut(s) 402, 496, 510, 1041, 1126
BssMI GATC 4 cut(s) 116, 424, 1218, 1333
BssT1I CCWWGG 1 cut(s) 1126
Bst2UI CCWGG 5 cut(s) 404, 498, 683, 912, 945
Bst4CI ACNGT 5 cut(s) 479, 971, 1006, 1081, 1247
Bst6I CTCTTC 2 cut(s) 490, 496
BstBI TTCGAA 1 cut(s) 1093
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 1371
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 5 cut(s) 39, 363, 578, 691, 1328
BstFNI CGCG 2 cut(s) 372, 512
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 2 cut(s) 374, 818
BstKTI GATC 4 cut(s) 119, 427, 1221, 1336
BstMAI GTCTC 1 cut(s) 960
BstMBI GATC 4 cut(s) 116, 424, 1218, 1333
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 5 cut(s) 164, 518, 527, 533, 536
BstNI CCWGG 5 cut(s) 404, 498, 683, 912, 945
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 6 cut(s) 402, 496, 681, 910, 943, 1040
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 2 cut(s) 372, 512
BstV1I GCAGC 5 cut(s) 268, 536, 548, 688, 1427
Bsu15I ATCGAT 1 cut(s) 1217
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 5 cut(s) 111, 247, 445, 542, 951
BsuTUI ATCGAT 1 cut(s) 1217
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 5 cut(s) 39, 363, 578, 691, 1328
BtsIMutI CAGTG 1 cut(s) 1064
Cac8I GCNNGC 1 cut(s) 156
CfoI GCGC 2 cut(s) 374, 818
Cfr13I GGNCC 2 cut(s) 1319, 1406
Cfr42I CCGCGG 1 cut(s) 513
ClaI ATCGAT 1 cut(s) 1217
CpoI CGGWCCG 1 cut(s) 1319
Csp6I GTAC 1 cut(s) 628
CspI CGGWCCG 1 cut(s) 1319
CviAII CATG 4 cut(s) 391, 596, 1156, 1367
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 1 cut(s) 1371
DpnI GATC 4 cut(s) 118, 426, 1220, 1335
DpnII GATC 4 cut(s) 116, 424, 1218, 1333
EaeI YGGCCR 1 cut(s) 245
Eam1104I CTCTTC 2 cut(s) 490, 496
EarI CTCTTC 2 cut(s) 490, 496
EciI GGCGGA 1 cut(s) 1332
Eco130I CCWWGG 1 cut(s) 1126
Eco24I GRGCYC 1 cut(s) 1377
Eco47I GGWCC 2 cut(s) 1319, 1406
Eco57I CTGAAG 1 cut(s) 525
EcoRI GAATTC 2 cut(s) 788, 1324
EcoRII CCWGG 5 cut(s) 402, 496, 681, 910, 943
EcoT14I CCWWGG 1 cut(s) 1126
EcoT38I GRGCYC 1 cut(s) 1377
ErhI CCWWGG 1 cut(s) 1126
FaeI CATG 4 cut(s) 394, 599, 1159, 1370
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 2 cut(s) 812, 1183
FatI CATG 4 cut(s) 390, 595, 1155, 1366
FauI CCCGC 1 cut(s) 505
FblI GTMKAC 1 cut(s) 338
FokI GGATG 5 cut(s) 46, 350, 565, 698, 1315
FriOI GRGCYC 1 cut(s) 1377
GlaI GCGC 2 cut(s) 373, 817
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 5 cut(s) 111, 247, 445, 542, 951
HapII CCGG 6 cut(s) 120, 334, 675, 1041, 1232, 1434
HhaI GCGC 2 cut(s) 374, 818
Hin1II CATG 4 cut(s) 394, 599, 1159, 1370
Hin6I GCGC 2 cut(s) 372, 816
HinP1I GCGC 2 cut(s) 372, 816
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 2 cut(s) 52, 822
HinfI GANTC 3 cut(s) 161, 566, 887
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 6 cut(s) 120, 334, 675, 1041, 1232, 1434
HphI GGTGA 3 cut(s) 236, 527, 1069
Hpy166II GTNNAC 5 cut(s) 125, 182, 316, 339, 1077
Hpy188I TCNGA 9 cut(s) 175, 424, 505, 838, 880, 1163, 1276, 1333, 1357
Hpy188III TCNNGA 4 cut(s) 65, 130, 465, 1434
Hpy8I GTNNAC 5 cut(s) 125, 182, 316, 339, 1077
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 2 cut(s) 101, 901
HpyCH4III ACNGT 5 cut(s) 479, 971, 1006, 1081, 1247
HpyCH4V TGCA 8 cut(s) 80, 284, 536, 599, 902, 1297, 1307, 1415
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 518, 527, 533, 536
HpyF3I CTNAG 1 cut(s) 1371
Hsp92II CATG 4 cut(s) 394, 599, 1159, 1370
HspAI GCGC 2 cut(s) 372, 816
Kpn2I TCCGGA 1 cut(s) 1433
KspAI GTTAAC 1 cut(s) 316
KspI CCGCGG 1 cut(s) 513
Kzo9I GATC 4 cut(s) 116, 424, 1218, 1333
LmnI GCTCC 2 cut(s) 671, 771
Lsp1109I GCAGC 5 cut(s) 268, 536, 548, 688, 1427
LweI GCATC 4 cut(s) 67, 208, 656, 897
MaeIII GTNAC 2 cut(s) 224, 988
MalI GATC 4 cut(s) 118, 426, 1220, 1335
MbiI CCGCTC 1 cut(s) 530
MboI GATC 4 cut(s) 116, 424, 1218, 1333
MboII GAAGA 5 cut(s) 300, 461, 477, 483, 1422
MfeI CAATTG 2 cut(s) 285, 1308
MhlI GDGCHC 1 cut(s) 1377
MlsI TGGCCA 1 cut(s) 247
MluNI TGGCCA 1 cut(s) 247
MmeI TCCRAC 2 cut(s) 1141, 1205
MnlI CCTC 9 cut(s) 179, 200, 435, 499, 553, 626, 1193, 1200, 1247
Mox20I TGGCCA 1 cut(s) 247
MroI TCCGGA 1 cut(s) 1433
MscI TGGCCA 1 cut(s) 247
MslI CAYNNNNRTG 1 cut(s) 1302
Msp20I TGGCCA 1 cut(s) 247
MspA1I CMGCKG 2 cut(s) 512, 539
MspI CCGG 6 cut(s) 120, 334, 675, 1041, 1232, 1434
MspR9I CCNGG 6 cut(s) 404, 498, 683, 912, 945, 1042
MunI CAATTG 2 cut(s) 285, 1308
MvaI CCWGG 5 cut(s) 404, 498, 683, 912, 945
MvnI CGCG 2 cut(s) 372, 512
MwoI GCNNNNNNNGC 5 cut(s) 164, 518, 527, 533, 536
NciI CCSGG 1 cut(s) 1042
NdeII GATC 4 cut(s) 116, 424, 1218, 1333
NlaIII CATG 4 cut(s) 394, 599, 1159, 1370
NlaIV GGNNCC 2 cut(s) 355, 773
NmuCI GTSAC 1 cut(s) 224
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 1 cut(s) 1093
PfeI GAWTC 3 cut(s) 161, 566, 887
PflMI CCANNNNNTGG 1 cut(s) 944
PsiI TTATAA 1 cut(s) 755
Psp6I CCWGG 5 cut(s) 402, 496, 681, 910, 943
PspGI CCWGG 5 cut(s) 402, 496, 681, 910, 943
PspN4I GGNNCC 2 cut(s) 355, 773
PspPI GGNCC 2 cut(s) 1319, 1406
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 1302
Rsr2I CGGWCCG 1 cut(s) 1319
RsrII CGGWCCG 1 cut(s) 1319
SacII CCGCGG 1 cut(s) 513
Sau3AI GATC 4 cut(s) 116, 424, 1218, 1333
Sau96I GGNCC 2 cut(s) 1319, 1406
ScrFI CCNGG 6 cut(s) 404, 498, 683, 912, 945, 1042
SduI GDGCHC 1 cut(s) 1377
SfaNI GCATC 4 cut(s) 67, 208, 656, 897
SfcI CTRYAG 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 1093
SgrBI CCGCGG 1 cut(s) 513
SinI GGWCC 2 cut(s) 1319, 1406
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 1 cut(s) 819
SmoI CTYRAG 1 cut(s) 819
SsiI CCGC 9 cut(s) 510, 512, 522, 528, 539, 704, 1062, 1317, 1395
StyD4I CCNGG 6 cut(s) 402, 496, 681, 910, 943, 1040
StyI CCWWGG 1 cut(s) 1126
TaaI ACNGT 5 cut(s) 479, 971, 1006, 1081, 1247
TaqI TCGA 4 cut(s) 564, 1067, 1093, 1217
TaqII GACCGA 2 cut(s) 550, 1336
TatI WGTACW 1 cut(s) 627
TauI GCSGC 6 cut(s) 512, 524, 530, 542, 707, 1397
TfiI GAWTC 3 cut(s) 161, 566, 887
TscAI CASTG 1 cut(s) 1064
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 5 cut(s) 281, 524, 536, 701, 1415
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 7 cut(s) 352, 567, 702, 801, 1157, 1251, 1317
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 1 cut(s) 1064
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 2 cut(s) 1319, 1406
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.