MD06G1091600.v1.1

cellulase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010905
Physical Location & Seq
Reverse (-)
22115641 .. 22121156
5516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1091600.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGAAAAAATATAAATTCCTTGAATCTCAGCAACTGCATGCAAACGCCACAAGCAAGTTACAAAATTTCAAACTGATCATCATGAAGCACGTCGTAGTACTGGTATGGGTTTTGGTCTTGTGTTGTGTCTTGGTTAACAAGGATGGGCAAGCCCTAGCCATGGAGGACGAAATTGAAGAGTTGGCAGTAGATCATGACTATAGAGATGCCCTCGGTAAGGCCATTTTATTCTTCGAAGGGCAACGCTCGGGAAAGTTGCCCACCAGCCAGCGAGTAAACTGGAGAGGAGACTCTGCACTCTCTGATGGAAAACCTCAAAATGTGAATTTGGCTGGAGGATACTATGATGCAGGTGACAACGTCAAGTTTGGGTGGCCAATGGCATTTAGTGTCAGCTTGTTGAGTTGGGCTGCTGTGGAGTACCAGCAGCAGATATCTTCTGCAAACCAGCTTGAATATCTCCATACTGCAATCCGTTGGGGCACAGATTACATACTGAAATGTCACTCTTCAGCCACCACATTTTATACCCAGGTGGGAGATGCAAATGCGGATCACCAGTGCTGGGAGCGCCCTGAGGACATGGATACACCACGAACACTTTACAAGATCACTTCTACTTCTCCTGGCTCCGAGCCAGCAGCTGAGGCTGCTGCTGCCCTTGCCGCTGCTTCACTTGTCTTTAAAGTGGTTGATTCCAACTACTCCGCGTTGCTCCTAAGCCATTCACGATCACTGTTTGACTTGGCAGATCAGCATAGAGCGTCTTACCAAGGCTATTGCCCATTTTACTGCTCCTACTCTGGCTATCAGGATGAGTTGTTATGGGCTGCGGCTTGGCTATACAAGGCGAGTGCAGACACCAACTATTTGAATTATGTCTCGAGCAACCAAGCATGGAGTCAGGCAGTGTCTGAGTTCAGTTGGGATAACAAATTAGTTGGAGCTCAGACATTACTAGCCAAGGAATTCTTCAGTGGAAATACGAATTTGGCTAAGTACAATAGCGATGTCCAGTCGTTTGTATGCGCACTGATGCCAGGGAGTAGTTCGGTACAGATCAGAAGAACCCCAGGTGGACTACTTTTTACAAGAGATAGTAGTAATTTGCAATATGTAACAAGCTCAACCCTGGTGCTATTCATCTACTCCAAAACCCTAACTGCAGCACAAATTGGTGGAGTCCAATGCGGCTCGGTCTTTTTCTCTACCTCCCAAATCAGAACCTTTGGAAAATATCAGGTGGACTACATCCTAGGAAGCAACCCCAAGAACATGTCATGCATGGTGGGTTTTGGTGGCAAATACCCAACGCAATTGCACCATAGAGGTTCATCAATCCCTTCCATCAACGTCCTCCCAGCCAAGGTGGATTGCAAAGGTGGTAACAACTATTTCTCCTCTTCCAATCCAAACCCTAATACTCATGTGGGTGCAATCGTTGGAGGTCCAGATGTAAACGACCAGTTCAACGATGCAAGATCAGACTATTCTCACTCTGAACCCACAACATATATGAATGCAGCTTTTGTGGGAACTGTGGCTGCTTTACTTGGTCAAGCCCGAACAGGGAGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

527

Amino Acids

57.72

Weight (kDa)

6.31

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 67 - 513 1.1e-153 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09740 AT4G23560
fragaria_vesca FvH4_5g03220
malus_domestica MD06G1091600.v1.1
prunus_persica Prupe.5G106800_v2.0.a1 Prupe.5G106800_v2.0.a1
pyrus_communis pycom06g08750
rosa_chinensis RchiOBHm_Chr7g0196541
rosa_laevigata RLG00000004006
rosa_multiflora Rmu_sc0013350.1_g000007
rosa_roxburghii Rroxscaffold_3G00259210
rosa_rugosa Rorug07G0034400 Rorug07G0034500 Rorug07G0034600
rosa_samantha Rh1BG041400 Rh7AG158700 Rh7BG160700 Rh7CG167100 Rh7DG159900
rosa_wichuraiana Rw7G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 341
Acc16I TGCGCA 1 cut(s) 1030
Acc36I ACCTGC 1 cut(s) 341
AccII CGCG 1 cut(s) 710
AciI CCGC 5 cut(s) 551, 666, 708, 833, 1191
AclWI GGATC 1 cut(s) 561
AcoI YGGCCR 1 cut(s) 374
AcsI RAATTY 5 cut(s) 14, 64, 325, 968, 988
AcuI CTGAAG 2 cut(s) 495, 958
AfaI GTAC 4 cut(s) 99, 422, 1001, 1056
AfiI CCNNNNNNNGG 5 cut(s) 160, 217, 565, 1366, 1569
AflIII ACRYGT 1 cut(s) 1275
AgsI TTSAA 6 cut(s) 23, 70, 176, 455, 874, 1471
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 5 cut(s) 531, 625, 1039, 1072, 1131
AjuI GAANNNNNNNTTGG 2 cut(s) 956, 988
AluBI AGCT 6 cut(s) 396, 451, 644, 947, 1125, 1526
AluI AGCT 6 cut(s) 396, 451, 644, 947, 1125, 1526
Alw21I GWGCWC 1 cut(s) 949
Alw26I GTCTC 2 cut(s) 282, 886
AlwI GGATC 1 cut(s) 561
AlwNI CAGNNNCTG 3 cut(s) 34, 644, 914
Ama87I CYCGRG 2 cut(s) 247, 883
AoxI GGCC 2 cut(s) 219, 374
ApoI RAATTY 5 cut(s) 14, 64, 325, 968, 988
AspA2I CCTAGG 1 cut(s) 1255
AspLEI GCGC 2 cut(s) 573, 1031
AspS9I GGNCC 1 cut(s) 1448
AsuHPI GGTGA 2 cut(s) 365, 548
AsuII TTCGAA 1 cut(s) 234
AvaI CYCGRG 2 cut(s) 247, 883
AvaII GGWCC 1 cut(s) 1448
AvrII CCTAGG 1 cut(s) 1255
AxyI CCTNAGG 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 485
BalI TGGCCA 1 cut(s) 376
BanII GRGCYC 1 cut(s) 949
Bbv12I GWGCWC 1 cut(s) 949
BbvCI CCTCAGC 1 cut(s) 645
BccI CCATC 3 cut(s) 137, 299, 1355
BciT130I CCWGG 5 cut(s) 533, 627, 1041, 1074, 1133
BciVI GTATCC 2 cut(s) 332, 580
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 282, 886
BfaI CTAG 3 cut(s) 155, 959, 1256
BfmI CTRYAG 2 cut(s) 199, 1164
BfoI RGCGCY 1 cut(s) 574
BfuAI ACCTGC 1 cut(s) 341
BfuI GTATCC 2 cut(s) 332, 580
BlnI CCTAGG 1 cut(s) 1255
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 5 cut(s) 533, 627, 1041, 1074, 1133
Bme18I GGWCC 1 cut(s) 1448
BmeT110I CYCGRG 2 cut(s) 247, 883
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 1448
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 5 cut(s) 533, 627, 1041, 1074, 1133
BmsI GCATC 5 cut(s) 196, 337, 532, 1026, 1465
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 2 cut(s) 301, 354
Bpu10I CCTNAGC 2 cut(s) 645, 719
Bpu14I TTCGAA 1 cut(s) 234
BsaXI ACNNNNNCTCC 2 cut(s) 1382, 1412
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 217, 565, 1366, 1569
Bse1I ACTGG 5 cut(s) 105, 284, 559, 1015, 1465
Bse21I CCTNAGG 1 cut(s) 576
BseBI CCWGG 5 cut(s) 533, 627, 1041, 1074, 1133
BseGI GGATG 3 cut(s) 148, 820, 1251
BseLI CCNNNNNNNGG 5 cut(s) 160, 217, 565, 1366, 1569
BseMII CTCAG 5 cut(s) 41, 567, 636, 906, 962
BseNI ACTGG 5 cut(s) 105, 284, 559, 1015, 1465
BseRI GAGGAG 2 cut(s) 300, 1390
BseSI GKGCMC 1 cut(s) 485
BseYI CCCAGC 2 cut(s) 564, 1360
BsgI GTGCAG 2 cut(s) 279, 876
Bsh1236I CGCG 1 cut(s) 710
BshFI GGCC 2 cut(s) 221, 376
BsiHKAI GWGCWC 1 cut(s) 949
BsiHKCI CYCGRG 2 cut(s) 247, 883
BslI CCNNNNNNNGG 5 cut(s) 160, 217, 565, 1366, 1569
BsmAI GTCTC 2 cut(s) 282, 886
BsmI GAATGC 1 cut(s) 1525
BsnI GGCC 2 cut(s) 221, 376
BsoBI CYCGRG 2 cut(s) 247, 883
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 485, 949
Bsp143I GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 5 cut(s) 551, 666, 708, 833, 1191
BspANI GGCC 2 cut(s) 221, 376
BspCNI CTCAG 5 cut(s) 40, 568, 637, 907, 961
BspFNI CGCG 1 cut(s) 710
BspHI TCATGA 2 cut(s) 81, 193
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 1168
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 1 cut(s) 561
BspT104I TTCGAA 1 cut(s) 234
BsrI ACTGG 5 cut(s) 105, 284, 559, 1015, 1465
BssMI GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
BssT1I CCWWGG 5 cut(s) 159, 772, 963, 1255, 1365
Bst2UI CCWGG 5 cut(s) 533, 627, 1041, 1074, 1133
Bst4CI ACNGT 2 cut(s) 738, 1540
Bst6I CTCTTC 3 cut(s) 171, 514, 1408
BstBI TTCGAA 1 cut(s) 234
BstC8I GCNNGC 4 cut(s) 39, 150, 269, 639
BstDEI CTNAG 7 cut(s) 27, 576, 645, 719, 915, 948, 996
BstDSI CCRYGG 1 cut(s) 159
BstENI CCTNNNNNAGG 1 cut(s) 215
BstF5I GGATG 3 cut(s) 148, 820, 1251
BstFNI CGCG 1 cut(s) 710
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 2 cut(s) 573, 1031
BstKTI GATC 8 cut(s) 78, 193, 556, 612, 734, 754, 1062, 1484
BstMAI GTCTC 2 cut(s) 282, 886
BstMBI GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
BstMWI GCNNNNNNNGC 6 cut(s) 570, 647, 650, 656, 662, 665
BstNI CCWGG 5 cut(s) 533, 627, 1041, 1074, 1133
BstNSI RCATGY 2 cut(s) 41, 1279
BstSCI CCNGG 5 cut(s) 531, 625, 1039, 1072, 1131
BstSFI CTRYAG 2 cut(s) 199, 1164
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 1 cut(s) 710
Bsu36I CCTNAGG 1 cut(s) 576
BsuI GTATCC 2 cut(s) 332, 580
BsuRI GGCC 2 cut(s) 221, 376
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 1023
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 3 cut(s) 148, 820, 1251
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 5 cut(s) 566, 734, 915, 982, 1031
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 4 cut(s) 39, 150, 269, 639
CaiI CAGNNNCTG 3 cut(s) 34, 644, 914
CciI TCATGA 2 cut(s) 81, 193
CfoI GCGC 2 cut(s) 573, 1031
Cfr13I GGNCC 1 cut(s) 1448
CseI GACGC 1 cut(s) 753
Csp6I GTAC 4 cut(s) 98, 421, 1000, 1055
CviQI GTAC 4 cut(s) 98, 421, 1000, 1055
DdeI CTNAG 7 cut(s) 27, 576, 645, 719, 915, 948, 996
DpnI GATC 8 cut(s) 77, 192, 555, 611, 733, 753, 1061, 1483
DpnII GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
DraI TTTAAA 1 cut(s) 685
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 3 cut(s) 171, 514, 1408
EarI CTCTTC 3 cut(s) 171, 514, 1408
Ecl136II GAGCTC 1 cut(s) 947
Eco130I CCWWGG 5 cut(s) 159, 772, 963, 1255, 1365
Eco24I GRGCYC 1 cut(s) 949
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 1448
Eco53kI GAGCTC 1 cut(s) 947
Eco57I CTGAAG 2 cut(s) 495, 958
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 2 cut(s) 247, 883
EcoICRI GAGCTC 1 cut(s) 947
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 968
EcoRII CCWGG 5 cut(s) 531, 625, 1039, 1072, 1131
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 5 cut(s) 159, 772, 963, 1255, 1365
EcoT22I ATGCAT 1 cut(s) 1286
EcoT38I GRGCYC 1 cut(s) 949
ErhI CCWWGG 5 cut(s) 159, 772, 963, 1255, 1365
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FbaI TGATCA 1 cut(s) 75
FokI GGATG 3 cut(s) 155, 827, 1238
FriOI GRGCYC 1 cut(s) 949
FspAI RTGCGCAY 1 cut(s) 1030
FspBI CTAG 3 cut(s) 155, 959, 1256
FspI TGCGCA 1 cut(s) 1030
GlaI GCGC 2 cut(s) 572, 1030
GsaI CCCAGC 2 cut(s) 568, 1364
GsuI CTGGAG 2 cut(s) 301, 354
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 2 cut(s) 221, 376
HgaI GACGC 1 cut(s) 753
HhaI GCGC 2 cut(s) 573, 1031
Hin6I GCGC 2 cut(s) 571, 1029
HinP1I GCGC 2 cut(s) 571, 1029
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 5 cut(s) 23, 290, 695, 901, 1182
HpaI GTTAAC 1 cut(s) 136
HphI GGTGA 2 cut(s) 365, 548
Hpy166II GTNNAC 5 cut(s) 136, 277, 1079, 1246, 1459
Hpy188I TCNGA 8 cut(s) 304, 634, 916, 951, 1064, 1223, 1486, 1501
Hpy188III TCNNGA 7 cut(s) 82, 194, 249, 729, 812, 883, 1451
Hpy8I GTNNAC 5 cut(s) 136, 277, 1079, 1246, 1459
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 230, 1353
HpyCH4III ACNGT 2 cut(s) 738, 1540
HpyCH4IV ACGT 3 cut(s) 90, 360, 1353
HpyF10VI GCNNNNNNNGC 6 cut(s) 570, 647, 650, 656, 662, 665
HpyF3I CTNAG 7 cut(s) 27, 576, 645, 719, 915, 948, 996
HpySE526I ACGT 3 cut(s) 90, 360, 1353
HspAI GCGC 2 cut(s) 571, 1029
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
LmnI GCTCC 5 cut(s) 568, 635, 720, 800, 944
LweI GCATC 5 cut(s) 196, 337, 532, 1026, 1465
MaeI CTAG 3 cut(s) 155, 959, 1256
MaeII ACGT 3 cut(s) 90, 360, 1353
MaeIII GTNAC 5 cut(s) 57, 353, 503, 1117, 1385
MalI GATC 8 cut(s) 77, 192, 555, 611, 733, 753, 1061, 1483
MboI GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
MboII GAAGA 7 cut(s) 188, 223, 429, 501, 964, 1077, 1395
MfeI CAATTG 1 cut(s) 1316
MhlI GDGCHC 2 cut(s) 485, 949
MlsI TGGCCA 1 cut(s) 376
MluNI TGGCCA 1 cut(s) 376
MlyI GAGTC 3 cut(s) 284, 910, 1191
MmeI TCCRAC 3 cut(s) 723, 922, 1423
Mox20I TGGCCA 1 cut(s) 376
Mph1103I ATGCAT 1 cut(s) 1286
MscI TGGCCA 1 cut(s) 376
MseI TTAA 2 cut(s) 135, 684
MslI CAYNNNNRTG 1 cut(s) 1431
Msp20I TGGCCA 1 cut(s) 376
MspA1I CMGCKG 2 cut(s) 644, 668
MspR9I CCNGG 5 cut(s) 533, 627, 1041, 1074, 1133
MunI CAATTG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1525
MvaI CCWGG 5 cut(s) 533, 627, 1041, 1074, 1133
MvnI CGCG 1 cut(s) 710
MwoI GCNNNNNNNGC 6 cut(s) 570, 647, 650, 656, 662, 665
NcoI CCATGG 1 cut(s) 159
NdeII GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
NlaIV GGNNCC 1 cut(s) 631
NmuCI GTSAC 2 cut(s) 353, 503
NsbI TGCGCA 1 cut(s) 1030
NsiI ATGCAT 1 cut(s) 1286
NspI RCATGY 2 cut(s) 41, 1279
NspV TTCGAA 1 cut(s) 234
PaeI GCATGC 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 883
PagI TCATGA 2 cut(s) 81, 193
PaqCI CACCTGC 1 cut(s) 341
PciI ACATGT 1 cut(s) 1275
PctI GAATGC 1 cut(s) 1525
PfeI GAWTC 2 cut(s) 23, 695
PflFI GACNNNGTC 1 cut(s) 359
PleI GAGTC 3 cut(s) 284, 909, 1190
PpsI GAGTC 3 cut(s) 284, 909, 1190
PscI ACATGT 1 cut(s) 1275
Psp124BI GAGCTC 1 cut(s) 949
Psp6I CCWGG 5 cut(s) 531, 625, 1039, 1072, 1131
PspFI CCCAGC 2 cut(s) 564, 1360
PspGI CCWGG 5 cut(s) 531, 625, 1039, 1072, 1131
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 1448
PstI CTGCAG 1 cut(s) 1168
PstNI CAGNNNCTG 3 cut(s) 34, 644, 914
PsyI GACNNNGTC 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 4 cut(s) 99, 422, 1001, 1056
RsaNI GTAC 4 cut(s) 98, 421, 1000, 1055
RseI CAYNNNNRTG 1 cut(s) 1431
SacI GAGCTC 1 cut(s) 949
SaqAI TTAA 2 cut(s) 135, 684
Sau3AI GATC 8 cut(s) 75, 190, 553, 609, 731, 751, 1059, 1481
Sau96I GGNCC 1 cut(s) 1448
ScaI AGTACT 1 cut(s) 99
SchI GAGTC 3 cut(s) 284, 910, 1191
ScrFI CCNGG 5 cut(s) 533, 627, 1041, 1074, 1133
SduI GDGCHC 2 cut(s) 485, 949
SfaNI GCATC 5 cut(s) 196, 337, 532, 1026, 1465
SfcI CTRYAG 2 cut(s) 199, 1164
Sfr274I CTCGAG 1 cut(s) 883
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 1448
SlaI CTCGAG 1 cut(s) 883
SmiMI CAYNNNNRTG 1 cut(s) 1431
SmlI CTYRAG 1 cut(s) 883
SmoI CTYRAG 1 cut(s) 883
SphI GCATGC 1 cut(s) 41
SsiI CCGC 5 cut(s) 551, 666, 708, 833, 1191
SspMI CTAG 3 cut(s) 155, 959, 1256
SstI GAGCTC 1 cut(s) 949
StyD4I CCNGG 5 cut(s) 531, 625, 1039, 1072, 1131
StyI CCWWGG 5 cut(s) 159, 772, 963, 1255, 1365
TaaI ACNGT 2 cut(s) 738, 1540
TaiI ACGT 3 cut(s) 93, 363, 1356
TaqI TCGA 2 cut(s) 234, 884
TaqII GACCGA 1 cut(s) 1186
TatI WGTACW 2 cut(s) 97, 999
TauI GCSGC 3 cut(s) 668, 836, 1194
TfiI GAWTC 2 cut(s) 23, 695
Tru1I TTAA 2 cut(s) 135, 684
Tru9I TTAA 2 cut(s) 135, 684
TscAI CASTG 5 cut(s) 566, 741, 915, 982, 1038
TseFI GTSAC 2 cut(s) 353, 503
Tsp45I GTSAC 2 cut(s) 353, 503
TspDTI ATGAA 5 cut(s) 17, 98, 1132, 1323, 1532
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 5 cut(s) 566, 741, 915, 982, 1038
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 1448
XagI CCTNNNNNAGG 1 cut(s) 215
XapI RAATTY 5 cut(s) 14, 64, 325, 968, 988
XceI RCATGY 2 cut(s) 41, 1279
XhoI CTCGAG 1 cut(s) 883
XmaJI CCTAGG 1 cut(s) 1255
XspI CTAG 3 cut(s) 155, 959, 1256
ZrmI AGTACT 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.