AT4G14420

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009048
Physical Location & Seq
Reverse (-)
8301851 .. 8303996
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14420.1

Sequence Viewer

Length: 477 bp
ATGGAGTTAGCTTCTTTCCTTGGTAGAGCTCTGTTTGTTTCCGTATTTCTTCTCTCCGCATGGCAAGAGTTCAATGATTTTGGTGAAGATGGTGGCCGATCAGCTAAATCTTTGAAACCAAAATTCAATGCCTTTGTAAACCATGTGACAACTCACACTGGCCAGCAATTGCCACCAGTCGATATGAAGATTCTTGTTGCTGCTGCTATAGCCTTGAAGGGTATTGGGGGACTATTGTTTGTCTTTGGCAGCTCATTGGGAGCTTATCTCTTGCTTCTGCATCAAGCCGTTGCCACCCCAATTCTGTATGATTTCTACAACTACGATGTTGACAGAAAGGAATTCGGCCAACTATTTTCAAAATTTACACAGAGCTTGGCTCTTCTTGGAGGACTGCTCTTCTTCATTGGAATGAAAAACTCAAGGAAACACGGTAGGCAACTCAGGAAAAAGGCTCCAAAGGCAAAAGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

158

Amino Acids

17.43

Weight (kDa)

9.94

Isoelectric Point (pI)

28.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 138 8.2e-66 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 3 cut(s) 94, 160, 346
AcsI RAATTY 3 cut(s) 122, 341, 362
AgsI TTSAA 5 cut(s) 73, 115, 127, 217, 360
AluBI AGCT 6 cut(s) 11, 29, 104, 252, 263, 375
AluI AGCT 6 cut(s) 11, 29, 104, 252, 263, 375
Alw21I GWGCWC 1 cut(s) 31
AoxI GGCC 3 cut(s) 94, 160, 346
ApeKI GCWGC 3 cut(s) 200, 203, 249
ApoI RAATTY 3 cut(s) 122, 341, 362
AsuHPI GGTGA 1 cut(s) 95
BalI TGGCCA 1 cut(s) 162
BanII GRGCYC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 187, 190, 261
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 272
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 3 cut(s) 201, 204, 250
BlsI GCNGC 3 cut(s) 202, 205, 251
BmiI GGNNCC 1 cut(s) 456
BmsI GCATC 1 cut(s) 289
BplI GAGNNNNNCTC 6 cut(s) 252, 284, 364, 396, 381, 413
BpuEI CTTGAG 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 163, 176
BseDI CCNNGG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 457
BseNI ACTGG 2 cut(s) 163, 176
BseXI GCAGC 3 cut(s) 187, 190, 261
BshFI GGCC 3 cut(s) 96, 162, 348
BsiHKAI GWGCWC 1 cut(s) 31
BslFI GGGAC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 243
BsnI GGCC 3 cut(s) 96, 162, 348
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 98
BspACI CCGC 1 cut(s) 57
BspANI GGCC 3 cut(s) 96, 162, 348
BspCNI CTCAG 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 456
BspQI GCTCTTC 2 cut(s) 387, 404
BsrI ACTGG 2 cut(s) 163, 176
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 2 cut(s) 387, 404
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 1 cut(s) 443
BstKTI GATC 1 cut(s) 101
BstMBI GATC 1 cut(s) 98
BstMWI GCNNNNNNNGC 2 cut(s) 209, 461
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 187, 190, 261
BsuRI GGCC 3 cut(s) 96, 162, 348
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 164
CviAII CATG 2 cut(s) 60, 143
DdeI CTNAG 1 cut(s) 443
DpnI GATC 1 cut(s) 100
DpnII GATC 1 cut(s) 98
EaeI YGGCCR 3 cut(s) 94, 160, 346
Eam1104I CTCTTC 2 cut(s) 387, 404
EarI CTCTTC 2 cut(s) 387, 404
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 2 cut(s) 63, 146
FaiI YATR 5 cut(s) 61, 144, 185, 209, 309
FaqI GGGAC 1 cut(s) 243
FatI CATG 2 cut(s) 59, 142
Fnu4HI GCNGC 3 cut(s) 201, 204, 250
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 201, 204, 250
GluI GCNGC 3 cut(s) 201, 204, 250
HaeIII GGCC 3 cut(s) 96, 162, 348
Hin1II CATG 2 cut(s) 63, 146
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 1 cut(s) 190
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 139, 331
Hpy188III TCNNGA 1 cut(s) 445
Hpy8I GTNNAC 2 cut(s) 139, 331
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 461
HpyF3I CTNAG 1 cut(s) 443
Hsp92II CATG 2 cut(s) 63, 146
Kzo9I GATC 1 cut(s) 98
LguI GCTCTTC 2 cut(s) 387, 404
LmnI GCTCC 2 cut(s) 260, 460
LpnPI CCDG 4 cut(s) 144, 176, 189, 430
Lsp1109I GCAGC 3 cut(s) 187, 190, 261
LweI GCATC 1 cut(s) 289
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 100
MboI GATC 1 cut(s) 98
MboII GAAGA 6 cut(s) 41, 98, 199, 374, 391, 394
MfeI CAATTG 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 5 cut(s) 122, 167, 300, 341, 362
MluNI TGGCCA 1 cut(s) 162
MnlI CCTC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 162
MscI TGGCCA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 410
Msp20I TGGCCA 1 cut(s) 162
MunI CAATTG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 209, 461
NdeII GATC 1 cut(s) 98
NlaIII CATG 2 cut(s) 63, 146
NlaIV GGNNCC 1 cut(s) 456
NmuCI GTSAC 1 cut(s) 145
PciSI GCTCTTC 2 cut(s) 387, 404
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 3 cut(s) 202, 205, 251
Psp124BI GAGCTC 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 456
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 31
SapI GCTCTTC 2 cut(s) 387, 404
SatI GCNGC 3 cut(s) 201, 204, 250
Sau3AI GATC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 13, 31, 106, 254, 265, 377
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 5 cut(s) 122, 167, 300, 341, 362
SsiI CCGC 1 cut(s) 57
SstI GAGCTC 1 cut(s) 31
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 180
TasI AATT 5 cut(s) 122, 167, 300, 341, 362
TfiI GAWTC 1 cut(s) 190
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 3 cut(s) 200, 203, 249
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 200, 394, 428
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 1 cut(s) 163
XapI RAATTY 3 cut(s) 122, 341, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.