RLG00000009850

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009048
Physical Location & Seq
Reverse (-)
58891756 .. 58894011
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009850

Sequence Viewer

Length: 474 bp
ATGGCCTTCGTTTCCTCCGTTGGGAGACTCCTTTTCGCCTCCGTCTTCCTTCTCTCCGCTTGGCAAGAGTTCAATGAATTTGGTTCGGATGGGGGACCTGCAGCTAAGTATTTGAGACCAAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCCAAGCAAGTTGAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTTGGGGGTGTTCTTTTCATCTTTGGAAGTTCTCTTGGAGCTGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTACGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCATTGTTTGGGGCTCTGCTCTTTTTCATAAGCATGAAGATATCAATGCCAAAGAGACTCAGGAAAAGAGCTCCCAAAGCTAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

158

Amino Acids

17.36

Weight (kDa)

9.94

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 7.2e-61 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 4 cut(s) 57, 203, 206, 332
AcsI RAATTY 3 cut(s) 77, 344, 376
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 2 cut(s) 21, 379
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 220, 349
AluBI AGCT 6 cut(s) 104, 124, 152, 266, 452, 461
AluI AGCT 6 cut(s) 104, 124, 152, 266, 452, 461
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 3 cut(s) 19, 109, 430
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 288
ApeKI GCWGC 2 cut(s) 101, 274
ApoI RAATTY 3 cut(s) 77, 344, 376
AsiGI ACCGGT 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 397, 454
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 113, 261
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 3 cut(s) 19, 109, 430
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 102, 204, 207, 275
BlsI GCNGC 4 cut(s) 103, 205, 208, 276
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 96
BmsI GCATC 2 cut(s) 292, 319
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 379
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 291
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 21, 379
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 454
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 2 cut(s) 113, 261
BshFI GGCC 2 cut(s) 5, 290
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 2 cut(s) 21, 379
BsmAI GTCTC 3 cut(s) 19, 109, 430
BsmFI GGGAC 1 cut(s) 108
BsnI GGCC 2 cut(s) 5, 290
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 174, 397, 454
BspACI CCGC 4 cut(s) 57, 203, 206, 332
BspANI GGCC 2 cut(s) 5, 290
BspCNI CTCAG 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 103
BspMI ACCTGC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 105, 440
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 3 cut(s) 19, 109, 430
BstMWI GCNNNNNNNGC 2 cut(s) 280, 458
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 113, 261
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 5, 290
BtsCI GGATG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 310
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 415, 471
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 2 cut(s) 105, 440
Ecl136II GAGCTC 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 397, 454
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 344
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 2 cut(s) 397, 454
FaeI CATG 2 cut(s) 418, 474
FaiI YATR 6 cut(s) 156, 305, 327, 410, 416, 472
FaqI GGGAC 1 cut(s) 108
FatI CATG 2 cut(s) 414, 470
Fnu4HI GCNGC 4 cut(s) 102, 204, 207, 275
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 2 cut(s) 397, 454
Fsp4HI GCNGC 4 cut(s) 102, 204, 207, 275
GluI GCNGC 4 cut(s) 102, 204, 207, 275
HaeIII GGCC 2 cut(s) 5, 290
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 418, 474
HinfI GANTC 2 cut(s) 27, 438
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 136, 217, 442
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 16, 59, 214
HpyCH4V TGCA 4 cut(s) 101, 166, 283, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 458
HpyF3I CTNAG 2 cut(s) 105, 440
Hsp92II CATG 2 cut(s) 418, 474
LmnI GCTCC 2 cut(s) 263, 457
LpnPI CCDG 5 cut(s) 111, 172, 272, 386, 427
Lsp1109I GCAGC 2 cut(s) 113, 261
LweI GCATC 2 cut(s) 292, 319
MboII GAAGA 2 cut(s) 37, 430
MhlI GDGCHC 3 cut(s) 174, 397, 454
MluCI AATT 3 cut(s) 77, 344, 376
MlyI GAGTC 2 cut(s) 21, 432
MnlI CCTC 3 cut(s) 25, 49, 371
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 159, 413
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 280, 458
NlaIII CATG 2 cut(s) 418, 474
NlaIV GGNNCC 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 4 cut(s) 103, 205, 208, 276
PleI GAGTC 2 cut(s) 21, 432
PpsI GAGTC 2 cut(s) 21, 432
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 95
PspPPI RGGWCCY 1 cut(s) 95
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 2 cut(s) 159, 413
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 102, 204, 207, 275
Sau96I GGNCC 1 cut(s) 95
SchI GAGTC 2 cut(s) 21, 432
SduI GDGCHC 3 cut(s) 174, 397, 454
SetI ASST 7 cut(s) 100, 106, 126, 154, 268, 454, 463
SfaNI GCATC 2 cut(s) 292, 319
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 413
SmlI CTYRAG 2 cut(s) 136, 362
SmoI CTYRAG 2 cut(s) 136, 362
Sse9I AATT 3 cut(s) 77, 344, 376
SsiI CCGC 4 cut(s) 57, 203, 206, 332
SstI GAGCTC 1 cut(s) 454
TaqI TCGA 2 cut(s) 126, 137
TasI AATT 3 cut(s) 77, 344, 376
TauI GCSGC 2 cut(s) 206, 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 2 cut(s) 101, 274
TspDTI ATGAA 4 cut(s) 90, 232, 397, 431
TspGWI ACGGA 2 cut(s) 7, 31
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 77, 344, 376
XhoI CTCGAG 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.