MD13G1210800.v1.1

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009048
Physical Location & Seq
Forward (+)
19613717 .. 19616229
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1210800.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCTTTCGTTTCGTTCGTTGGACGCCTTCTTTTCGCCTCTGTCTTCTTCCTCTCTGCTTGGCATGAGTTCAATGAATTTGGCGTGGACGGGGGACCCGCAGCAGAATATCTAAGACCAAAATTCGACGTTTTCTCGAATCATCTGTCAACTCACACTGGTGTGCAAGTGCCCAAACAAGTTGAAATCAAACATTTAGTGGCGGCCATGGTGGCATTGAAGGGCATTGGGGGTCTTCTCTTCATCTTTGGTTCTTCTATTGGAGCCTATTTGCTGCTTCTGCATCAGGCCATTGCTACTCCTATCTTGTACGATTTCTACAACTATGATCCCGAGAAGACAGAATTCAATCAACTCTTTTTTCAGTTTACTCAGAACTTGGCATTCTTCGGGGCCCTGCTTTTTTTCGTAGGCATGAAGACATCGATACCAAAGAGACAACAACTCAGGAAAAGAGTTCCAAAACCAAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

160

Amino Acids

17.96

Weight (kDa)

9.56

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 7e-64 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 99, 203
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 3 cut(s) 77, 122, 344
AcyI GRCGYC 1 cut(s) 25
AfaI GTAC 1 cut(s) 311
AgsI TTSAA 4 cut(s) 73, 185, 220, 349
AleI CACNNNNGTG 2 cut(s) 159, 161
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 3 cut(s) 204, 288, 393
ApaI GGGCCC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 101, 274
ApoI RAATTY 3 cut(s) 77, 122, 344
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspS9I GGNCC 3 cut(s) 95, 393, 394
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 2 cut(s) 174, 397
BanII GRGCYC 1 cut(s) 397
BbsI GAAGAC 4 cut(s) 37, 227, 344, 425
BbvI GCAGC 2 cut(s) 113, 261
BcoDI GTCTC 1 cut(s) 430
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 3 cut(s) 102, 204, 275
BlsI GCNGC 3 cut(s) 103, 205, 276
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 3 cut(s) 95, 393, 394
BmiI GGNNCC 5 cut(s) 96, 97, 265, 394, 395
BmsI GCATC 1 cut(s) 292
BpiI GAAGAC 4 cut(s) 37, 227, 344, 425
Bsa29I ATCGAT 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 386, 460
BseNI ACTGG 1 cut(s) 163
BseSI GKGCMC 2 cut(s) 174, 397
BseXI GCAGC 2 cut(s) 113, 261
BshFI GGCC 3 cut(s) 206, 290, 395
BshVI ATCGAT 1 cut(s) 425
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 3 cut(s) 206, 290, 395
BsoBI CYCGRG 1 cut(s) 332
Bsp120I GGGCCC 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 174, 397
Bsp143I GATC 1 cut(s) 328
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 2 cut(s) 99, 203
BspANI GGCC 3 cut(s) 206, 290, 395
BspCNI CTCAG 2 cut(s) 385, 459
BspDI ATCGAT 1 cut(s) 425
BspLI GGNNCC 5 cut(s) 96, 97, 265, 394, 395
BspPI GGATC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 1 cut(s) 328
BssNI GRCGYC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 25
BstDEI CTNAG 3 cut(s) 113, 372, 446
BstDSI CCRYGG 1 cut(s) 207
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 2 cut(s) 212, 280
BstSLI GKGCMC 2 cut(s) 174, 397
BstV1I GCAGC 2 cut(s) 113, 261
BstV2I GAAGAC 4 cut(s) 37, 227, 344, 425
Bsu15I ATCGAT 1 cut(s) 425
BsuRI GGCC 3 cut(s) 206, 290, 395
BsuTUI ATCGAT 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 95, 393, 394
ClaI ATCGAT 1 cut(s) 425
CseI GACGC 1 cut(s) 33
Csp6I GTAC 1 cut(s) 310
CviAII CATG 4 cut(s) 65, 208, 415, 477
CviJI RGCY 5 cut(s) 5, 206, 266, 290, 395
CviKI_1 RGCY 5 cut(s) 5, 206, 266, 290, 395
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 3 cut(s) 113, 372, 446
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 95
Eco88I CYCGRG 1 cut(s) 332
EcoO109I RGGNCCY 3 cut(s) 95, 393, 394
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 4 cut(s) 68, 211, 418, 480
FaiI YATR 5 cut(s) 66, 209, 327, 416, 478
FaqI GGGAC 1 cut(s) 108
FatI CATG 4 cut(s) 64, 207, 414, 476
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 102, 204, 275
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 3 cut(s) 102, 204, 275
GluI GCNGC 3 cut(s) 102, 204, 275
HaeIII GGCC 3 cut(s) 206, 290, 395
HgaI GACGC 1 cut(s) 33
Hin1I GRCGYC 1 cut(s) 25
Hin1II CATG 4 cut(s) 68, 211, 418, 480
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 1 cut(s) 139
Hpy166II GTNNAC 3 cut(s) 88, 150, 369
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 3 cut(s) 136, 332, 448
Hpy8I GTNNAC 3 cut(s) 88, 150, 369
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 38, 214
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 166, 283
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 280
HpyF3I CTNAG 3 cut(s) 113, 372, 446
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 25
Hsp92II CATG 4 cut(s) 68, 211, 418, 480
KflI GGGWCCC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 4 cut(s) 144, 272, 410, 433
Lsp1109I GCAGC 2 cut(s) 113, 261
LweI GCATC 1 cut(s) 292
MaeII ACGT 1 cut(s) 129
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 8 cut(s) 37, 40, 227, 232, 246, 349, 379, 430
MhlI GDGCHC 2 cut(s) 174, 397
MluCI AATT 3 cut(s) 77, 122, 344
MnlI CCTC 2 cut(s) 49, 62
MslI CAYNNNNRTG 2 cut(s) 159, 161
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 212, 280
NcoI CCATGG 1 cut(s) 207
NdeII GATC 1 cut(s) 328
NlaIII CATG 4 cut(s) 68, 211, 418, 480
NlaIV GGNNCC 5 cut(s) 96, 97, 265, 394, 395
OliI CACNNNNGTG 2 cut(s) 159, 161
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 3 cut(s) 103, 205, 276
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 5 cut(s) 96, 97, 265, 394, 395
PspOMI GGGCCC 1 cut(s) 393
PspPI GGNCC 3 cut(s) 95, 393, 394
PspPPI RGGWCCY 1 cut(s) 95
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 2 cut(s) 159, 161
SatI GCNGC 3 cut(s) 102, 204, 275
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 3 cut(s) 95, 393, 394
SduI GDGCHC 2 cut(s) 174, 397
SetI ASST 1 cut(s) 132
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 1 cut(s) 95
SmiMI CAYNNNNRTG 2 cut(s) 159, 161
Sse9I AATT 3 cut(s) 77, 122, 344
SsiI CCGC 2 cut(s) 99, 203
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 126, 137, 425
TasI AATT 3 cut(s) 77, 122, 344
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 139
TscAI CASTG 1 cut(s) 163
TseI GCWGC 2 cut(s) 101, 274
TspDTI ATGAA 3 cut(s) 90, 232, 431
TspRI CASTG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 77, 122, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.