Rh4AG039200

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009048
Physical Location & Seq
Forward (+)
8235853 .. 8237439
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG039200.1

Sequence Viewer

Length: 474 bp
ATGGCCTTCGTTTCCTCCGCTGGGAGGCTCCTTTTCGCCTCCGTCTTCCTTCTCTCCGCTTGGCAAGAGTTCAATGAATTTGGTTCGGATGGGGGAGCTGCAGCTAAGTATTTGAGACCAAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCGAAGCAAGTTGAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTCGGGGGTGTTCTTTTCATCTTTGGAAGTACTCTCGGAGCTGTTCTACTGCTTCTGCATCAGGCCATGGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCATTGTTTGGGGCTCTGCTCTTTTTCATAAGCATGAAGATATCAATGCCAAAGAGACTCAGGAAAAGAGCTCCCAAAGCTAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

157

Amino Acids

17.34

Weight (kDa)

9.94

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 4e-59 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 5 cut(s) 18, 57, 203, 206, 332
AcsI RAATTY 3 cut(s) 77, 344, 376
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 3 cut(s) 21, 24, 379
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 220, 349
AluBI AGCT 7 cut(s) 98, 104, 124, 152, 266, 452, 461
AluI AGCT 7 cut(s) 98, 104, 124, 152, 266, 452, 461
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 2 cut(s) 109, 430
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 288
ApeKI GCWGC 2 cut(s) 98, 101
ApoI RAATTY 3 cut(s) 77, 344, 376
AsiGI ACCGGT 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 136
BanII GRGCYC 2 cut(s) 397, 454
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 85, 113
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 2 cut(s) 109, 430
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 4 cut(s) 99, 102, 204, 207
BlsI GCNGC 4 cut(s) 100, 103, 205, 208
BmcAI AGTACT 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 2 cut(s) 292, 319
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 24, 379
Bse118I RCCGGY 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 3 cut(s) 21, 24, 379
BseMII CTCAG 1 cut(s) 454
BseXI GCAGC 2 cut(s) 85, 113
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 2 cut(s) 5, 290
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 21, 24, 379
BsmAI GTCTC 2 cut(s) 109, 430
BsnI GGCC 2 cut(s) 5, 290
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 397, 454
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 5 cut(s) 18, 57, 203, 206, 332
BspANI GGCC 2 cut(s) 5, 290
BspCNI CTCAG 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 29
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 105, 440
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 2 cut(s) 109, 430
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 85, 113
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 5, 290
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 255
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 3 cut(s) 292, 415, 471
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 105, 440
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 2 cut(s) 397, 454
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 344
EcoRV GATATC 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 397, 454
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 3 cut(s) 295, 418, 474
FaiI YATR 8 cut(s) 156, 293, 305, 312, 327, 410, 416, 472
FatI CATG 3 cut(s) 291, 414, 470
Fnu4HI GCNGC 4 cut(s) 99, 102, 204, 207
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 2 cut(s) 397, 454
Fsp4HI GCNGC 4 cut(s) 99, 102, 204, 207
GluI GCNGC 4 cut(s) 99, 102, 204, 207
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 5, 290
HapII CCGG 1 cut(s) 159
Hin1II CATG 3 cut(s) 295, 418, 474
HinfI GANTC 1 cut(s) 438
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 2 cut(s) 88, 263
Hpy188III TCNNGA 3 cut(s) 136, 217, 442
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 16, 59, 214
HpyCH4V TGCA 3 cut(s) 101, 166, 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 2 cut(s) 105, 440
Hsp92II CATG 3 cut(s) 295, 418, 474
LmnI GCTCC 4 cut(s) 33, 95, 263, 457
LpnPI CCDG 5 cut(s) 6, 172, 272, 386, 427
Lsp1109I GCAGC 2 cut(s) 85, 113
LweI GCATC 2 cut(s) 292, 319
MboII GAAGA 2 cut(s) 37, 430
MhlI GDGCHC 2 cut(s) 397, 454
MluCI AATT 3 cut(s) 77, 344, 376
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 4 cut(s) 18, 25, 49, 371
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 159, 413
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 458
NcoI CCATGG 1 cut(s) 291
NlaIII CATG 3 cut(s) 295, 418, 474
NlaIV GGNNCC 1 cut(s) 29
PaeR7I CTCGAG 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 4 cut(s) 100, 103, 205, 208
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 29
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 2 cut(s) 159, 413
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 99, 102, 204, 207
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 1 cut(s) 432
SduI GDGCHC 2 cut(s) 397, 454
SetI ASST 7 cut(s) 100, 106, 126, 154, 268, 454, 463
SfaNI GCATC 2 cut(s) 292, 319
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 413
SmlI CTYRAG 2 cut(s) 136, 362
SmoI CTYRAG 2 cut(s) 136, 362
Sse9I AATT 3 cut(s) 77, 344, 376
SsiI CCGC 5 cut(s) 18, 57, 203, 206, 332
SstI GAGCTC 1 cut(s) 454
StyI CCWWGG 1 cut(s) 291
TaqI TCGA 2 cut(s) 126, 137
TasI AATT 3 cut(s) 77, 344, 376
TatI WGTACW 1 cut(s) 254
TauI GCSGC 2 cut(s) 206, 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 2 cut(s) 98, 101
TspDTI ATGAA 4 cut(s) 90, 232, 397, 431
TspGWI ACGGA 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 379
XapI RAATTY 3 cut(s) 77, 344, 376
XhoI CTCGAG 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.