FvH4_4g03230

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009048
Physical Location & Seq
Forward (+)
2877438 .. 2879517
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03230.t2

Sequence Viewer

Length: 477 bp
ATGGCCTTCGTTTCCTCCGTCGGGAGACTCCTATTCGCCTCCGTCTTCCTTCTCTCTGCTTGGCAAGAGTTCAATGAGTTTGGTTCGGATGGGGGCCCTGCAGCCAAGTTTCTGAGACCGAAGCTCGATGTTTTCTCTAGCCATCTGTTGACTCACACCGGCATGCATCTGCCCAAGCAACTTGAGATTAAGCATTTAGTGGCGGCAGCGATCGTCTTGAAGGGTGTTGGGGGTCTTCTTTTCATTTTTGGAAGTTCTTTTGGAGCCGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCATTGTTTGGGGCTCTACTCTTTTTCATTAGCATGAAGATATCAATGCCAAAGAAACAGCTCAGGAAAAGAGCTCCCAAAGCGAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

159

Amino Acids

17.6

Weight (kDa)

9.95

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 5 - 124 3.3e-49 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 2 cut(s) 203, 332
AcsI RAATTY 2 cut(s) 344, 376
AfiI CCNNNNNNNGG 2 cut(s) 21, 379
AgsI TTSAA 3 cut(s) 73, 220, 349
AluBI AGCT 3 cut(s) 124, 442, 455
AluI AGCT 3 cut(s) 124, 442, 455
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 19, 109
AoxI GGCC 3 cut(s) 3, 94, 288
ApaI GGGCCC 1 cut(s) 98
ApeKI GCWGC 3 cut(s) 101, 206, 274
ApoI RAATTY 2 cut(s) 344, 376
AspS9I GGNCC 2 cut(s) 94, 95
BaeGI GKGCMC 1 cut(s) 98
BanII GRGCYC 3 cut(s) 98, 397, 457
BbsI GAAGAC 2 cut(s) 37, 227
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 3 cut(s) 113, 218, 261
BccI CCATC 2 cut(s) 83, 150
BceAI ACGGC 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 19, 109
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 4 cut(s) 102, 204, 207, 275
BlsI GCNGC 4 cut(s) 103, 205, 208, 276
BmgT120I GGNCC 2 cut(s) 94, 95
BmiI GGNNCC 3 cut(s) 95, 96, 265
BmsI GCATC 3 cut(s) 175, 292, 319
BpiI GAAGAC 2 cut(s) 37, 227
Bpu10I CCTNAGC 1 cut(s) 443
BpuEI CTTGAG 2 cut(s) 203, 347
BsaI GGTCTC 1 cut(s) 109
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 379
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 291
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 21, 379
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 104, 457
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 3 cut(s) 113, 218, 261
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 5, 96, 290
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 457
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 21, 379
BsmAI GTCTC 2 cut(s) 19, 109
BsnI GGCC 3 cut(s) 5, 96, 290
Bso31I GGTCTC 1 cut(s) 109
Bsp120I GGGCCC 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 98, 397, 457
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 2 cut(s) 203, 332
BspANI GGCC 3 cut(s) 5, 96, 290
BspCNI CTCAG 2 cut(s) 105, 456
BspLI GGNNCC 3 cut(s) 95, 96, 265
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssMI GATC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 2 cut(s) 113, 443
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 1 cut(s) 213
BstMAI GTCTC 2 cut(s) 19, 109
BstMBI GATC 1 cut(s) 210
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 2 cut(s) 280, 461
BstNSI RCATGY 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 98
BstV1I GCAGC 3 cut(s) 113, 218, 261
BstV2I GAAGAC 2 cut(s) 37, 227
BsuRI GGCC 3 cut(s) 5, 96, 290
BtsCI GGATG 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 94, 95
CviAII CATG 3 cut(s) 163, 415, 474
DdeI CTNAG 2 cut(s) 113, 443
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 455
Eco24I GRGCYC 3 cut(s) 98, 397, 457
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 455
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 2 cut(s) 94, 95
EcoRI GAATTC 1 cut(s) 344
EcoRV GATATC 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 168
EcoT38I GRGCYC 3 cut(s) 98, 397, 457
FaeI CATG 3 cut(s) 166, 418, 477
FaiI YATR 6 cut(s) 164, 305, 312, 327, 416, 475
FatI CATG 3 cut(s) 162, 414, 473
Fnu4HI GCNGC 4 cut(s) 102, 204, 207, 275
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 3 cut(s) 98, 397, 457
Fsp4HI GCNGC 4 cut(s) 102, 204, 207, 275
FspBI CTAG 1 cut(s) 138
GluI GCNGC 4 cut(s) 102, 204, 207, 275
HaeIII GGCC 3 cut(s) 5, 96, 290
HapII CCGG 1 cut(s) 159
Hin1II CATG 3 cut(s) 166, 418, 477
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 2 cut(s) 27, 151
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 2 cut(s) 150, 369
Hpy188I TCNGA 2 cut(s) 88, 114
Hpy188III TCNNGA 3 cut(s) 22, 217, 445
Hpy8I GTNNAC 2 cut(s) 150, 369
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 16, 59, 214
HpyCH4V TGCA 4 cut(s) 101, 166, 283, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 461
HpyF3I CTNAG 2 cut(s) 113, 443
Hsp92II CATG 3 cut(s) 166, 418, 477
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 263, 460
LpnPI CCDG 5 cut(s) 111, 172, 272, 386, 430
Lsp1109I GCAGC 3 cut(s) 113, 218, 261
LweI GCATC 3 cut(s) 175, 292, 319
MaeI CTAG 1 cut(s) 138
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 3 cut(s) 37, 227, 430
MhlI GDGCHC 3 cut(s) 98, 397, 457
MluCI AATT 2 cut(s) 344, 376
MlyI GAGTC 2 cut(s) 21, 145
MnlI CCTC 3 cut(s) 25, 49, 371
Mph1103I ATGCAT 1 cut(s) 168
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 161, 413
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 280, 461
NdeII GATC 1 cut(s) 210
NlaIII CATG 3 cut(s) 166, 418, 477
NlaIV GGNNCC 3 cut(s) 95, 96, 265
NsiI ATGCAT 1 cut(s) 168
NspI RCATGY 1 cut(s) 166
PaeI GCATGC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 4 cut(s) 103, 205, 208, 276
Ple19I CGATCG 1 cut(s) 213
PleI GAGTC 2 cut(s) 21, 145
PpsI GAGTC 2 cut(s) 21, 145
Psp124BI GAGCTC 1 cut(s) 457
PspN4I GGNNCC 3 cut(s) 95, 96, 265
PspOMI GGGCCC 1 cut(s) 94
PspPI GGNCC 2 cut(s) 94, 95
PstI CTGCAG 1 cut(s) 103
PvuI CGATCG 1 cut(s) 213
RseI CAYNNNNRTG 2 cut(s) 161, 413
SacI GAGCTC 1 cut(s) 457
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 102, 204, 207, 275
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 2 cut(s) 94, 95
SchI GAGTC 2 cut(s) 21, 145
SduI GDGCHC 3 cut(s) 98, 397, 457
SetI ASST 3 cut(s) 126, 444, 457
SfaNI GCATC 3 cut(s) 175, 292, 319
SfcI CTRYAG 1 cut(s) 99
SmiMI CAYNNNNRTG 2 cut(s) 161, 413
SmlI CTYRAG 2 cut(s) 182, 362
SmoI CTYRAG 2 cut(s) 182, 362
SphI GCATGC 1 cut(s) 166
Sse9I AATT 2 cut(s) 344, 376
SsiI CCGC 2 cut(s) 203, 332
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 457
TaqI TCGA 1 cut(s) 126
TaqII GACCGA 1 cut(s) 133
TasI AATT 2 cut(s) 344, 376
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 3 cut(s) 101, 206, 274
TspDTI ATGAA 3 cut(s) 232, 397, 431
TspGWI ACGGA 2 cut(s) 7, 31
Van91I CCANNNNNTGG 1 cut(s) 379
XapI RAATTY 2 cut(s) 344, 376
XceI RCATGY 1 cut(s) 166
XspI CTAG 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.