Rw4G003060

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009048
Physical Location & Seq
Forward (+)
6139739 .. 6141932
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003060.1

Sequence Viewer

Length: 477 bp
ATGGCCTTCGTTTCCTCCGTTGGGAGACTCCTTTTCGCCTCCGTCTTCCTTCTCTCCGCTTGGCAAGAGTTCAATGAATTTGGTTCGGATGGGGGAGCTGCAGCTAAGTATTTGAGACCGAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCCAAGCAAGTTGAGCAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTCGGGGGTGTTCTTTTCATCTTTGGAAGTACTCTTGGAGCTGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCATTGTTTGGGGCTCTGCTTTTTTTCATAAGCATGAAGATATCAATGCCAAAGAGACTCAGGAAAAGAACTCCCAAAGCTAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

158

Amino Acids

17.51

Weight (kDa)

9.94

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 140 2.1e-58 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 382
AciI CCGC 4 cut(s) 57, 206, 209, 335
AcsI RAATTY 3 cut(s) 77, 347, 379
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 2 cut(s) 21, 382
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 223, 352
AluBI AGCT 6 cut(s) 98, 104, 124, 152, 269, 464
AluI AGCT 6 cut(s) 98, 104, 124, 152, 269, 464
Alw26I GTCTC 3 cut(s) 19, 109, 433
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 291
ApeKI GCWGC 3 cut(s) 98, 101, 277
ApoI RAATTY 3 cut(s) 77, 347, 379
AsiGI ACCGGT 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 37
BbvI GCAGC 3 cut(s) 85, 113, 264
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 3 cut(s) 19, 109, 433
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 5 cut(s) 99, 102, 207, 210, 278
BlsI GCNGC 5 cut(s) 100, 103, 208, 211, 279
BmcAI AGTACT 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 136
BmsI GCATC 2 cut(s) 295, 322
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 382
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 294
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 21, 382
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 3 cut(s) 85, 113, 264
BshFI GGCC 2 cut(s) 5, 293
BshTI ACCGGT 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 21, 382
BsmAI GTCTC 3 cut(s) 19, 109, 433
BsnI GGCC 2 cut(s) 5, 293
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 174, 400
BspACI CCGC 4 cut(s) 57, 206, 209, 335
BspANI GGCC 2 cut(s) 5, 293
BspCNI CTCAG 1 cut(s) 456
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 105, 443
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 3 cut(s) 19, 109, 433
BstMWI GCNNNNNNNGC 3 cut(s) 184, 193, 283
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 85, 113, 264
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 5, 293
BtsCI GGATG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 258
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 418, 474
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 2 cut(s) 105, 443
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 347
EcoRV GATATC 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 400
FaeI CATG 2 cut(s) 421, 477
FaiI YATR 7 cut(s) 156, 308, 315, 330, 413, 419, 475
FatI CATG 2 cut(s) 417, 473
Fnu4HI GCNGC 5 cut(s) 99, 102, 207, 210, 278
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 5 cut(s) 99, 102, 207, 210, 278
GluI GCNGC 5 cut(s) 99, 102, 207, 210, 278
HaeIII GGCC 2 cut(s) 5, 293
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 421, 477
HinfI GANTC 2 cut(s) 27, 441
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 136, 220, 445
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 16, 59, 217
HpyCH4V TGCA 4 cut(s) 101, 166, 286, 299
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 193, 283
HpyF3I CTNAG 2 cut(s) 105, 443
Hsp92II CATG 2 cut(s) 421, 477
LmnI GCTCC 2 cut(s) 95, 266
LpnPI CCDG 4 cut(s) 172, 275, 389, 430
Lsp1109I GCAGC 3 cut(s) 85, 113, 264
LweI GCATC 2 cut(s) 295, 322
MboII GAAGA 2 cut(s) 37, 433
MhlI GDGCHC 2 cut(s) 174, 400
MluCI AATT 3 cut(s) 77, 347, 379
MlyI GAGTC 2 cut(s) 21, 435
MnlI CCTC 3 cut(s) 25, 49, 374
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 159, 416
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 184, 193, 283
NlaIII CATG 2 cut(s) 421, 477
PaeR7I CTCGAG 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 382
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 5 cut(s) 100, 103, 208, 211, 279
PleI GAGTC 2 cut(s) 21, 435
PpsI GAGTC 2 cut(s) 21, 435
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
RseI CAYNNNNRTG 2 cut(s) 159, 416
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 5 cut(s) 99, 102, 207, 210, 278
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 2 cut(s) 21, 435
SduI GDGCHC 2 cut(s) 174, 400
SetI ASST 6 cut(s) 100, 106, 126, 154, 271, 466
SfaNI GCATC 2 cut(s) 295, 322
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 416
SmlI CTYRAG 2 cut(s) 136, 365
SmoI CTYRAG 2 cut(s) 136, 365
Sse9I AATT 3 cut(s) 77, 347, 379
SsiI CCGC 4 cut(s) 57, 206, 209, 335
TaqI TCGA 2 cut(s) 126, 137
TaqII GACCGA 1 cut(s) 133
TasI AATT 3 cut(s) 77, 347, 379
TatI WGTACW 1 cut(s) 257
TauI GCSGC 2 cut(s) 209, 212
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 3 cut(s) 98, 101, 277
TspDTI ATGAA 4 cut(s) 90, 235, 400, 434
TspGWI ACGGA 2 cut(s) 7, 31
Van91I CCANNNNNTGG 1 cut(s) 382
XapI RAATTY 3 cut(s) 77, 347, 379
XhoI CTCGAG 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.