RchiOBHm_Chr4g0391911

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009048
Physical Location & Seq
Forward (+)
7312610 .. 7315849
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36469

Sequence Viewer

Length: 474 bp
ATGGCCTTCGTTTCTTCCGCTGGGAGACTCCTTTTCGCCTCCGTCTTCCTTCTCTCCGCTTGGCAAGAGTTCAATGAATTTGGTTCGGATGGGGGAGCTGCAGCTAAGTATTTGAGACCAAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCCAAGCAAGTTGAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTCGGGGGTGTTCTTTTCATCTTTGGAAGTACTCTTGGAGCTGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCCTTGTTTGGGGCTCTGCTCTTTTTCATAAGCATGAAGATATCAATGCCAAAGAGACTCAGGAAAAGAGCTCCCAAAGCTAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

157

Amino Acids

17.32

Weight (kDa)

9.94

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 2.3e-59 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 5 cut(s) 18, 57, 203, 206, 332
AcsI RAATTY 3 cut(s) 77, 344, 376
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 2 cut(s) 379, 390
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 220, 349
AluBI AGCT 7 cut(s) 98, 104, 124, 152, 266, 452, 461
AluI AGCT 7 cut(s) 98, 104, 124, 152, 266, 452, 461
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 3 cut(s) 19, 109, 430
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 3 cut(s) 3, 288, 381
ApeKI GCWGC 3 cut(s) 98, 101, 274
ApoI RAATTY 3 cut(s) 77, 344, 376
AsiGI ACCGGT 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 397, 454
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 3 cut(s) 85, 113, 261
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 3 cut(s) 19, 109, 430
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 5 cut(s) 99, 102, 204, 207, 275
BlsI GCNGC 5 cut(s) 100, 103, 205, 208, 276
BmcAI AGTACT 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 136
BmsI GCATC 2 cut(s) 292, 319
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 390
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 291
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 379, 390
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 454
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 3 cut(s) 85, 113, 261
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 3 cut(s) 5, 290, 383
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 379, 390
BsmAI GTCTC 3 cut(s) 19, 109, 430
BsnI GGCC 3 cut(s) 5, 290, 383
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 174, 397, 454
BspACI CCGC 5 cut(s) 18, 57, 203, 206, 332
BspANI GGCC 3 cut(s) 5, 290, 383
BspCNI CTCAG 1 cut(s) 453
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 105, 440
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 3 cut(s) 19, 109, 430
BstMWI GCNNNNNNNGC 2 cut(s) 280, 458
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 85, 113, 261
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 3 cut(s) 5, 290, 383
BtsCI GGATG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 255
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 415, 471
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 105, 440
Ecl136II GAGCTC 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 397, 454
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 344
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 2 cut(s) 397, 454
FaeI CATG 2 cut(s) 418, 474
FaiI YATR 7 cut(s) 156, 305, 312, 327, 410, 416, 472
FatI CATG 2 cut(s) 414, 470
Fnu4HI GCNGC 5 cut(s) 99, 102, 204, 207, 275
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 2 cut(s) 397, 454
Fsp4HI GCNGC 5 cut(s) 99, 102, 204, 207, 275
GluI GCNGC 5 cut(s) 99, 102, 204, 207, 275
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 3 cut(s) 5, 290, 383
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 418, 474
HinfI GANTC 2 cut(s) 27, 438
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 136, 217, 442
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 16, 59, 214
HpyCH4V TGCA 4 cut(s) 101, 166, 283, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 458
HpyF3I CTNAG 2 cut(s) 105, 440
Hsp92II CATG 2 cut(s) 418, 474
LmnI GCTCC 3 cut(s) 95, 263, 457
LpnPI CCDG 5 cut(s) 6, 172, 272, 386, 427
Lsp1109I GCAGC 3 cut(s) 85, 113, 261
LweI GCATC 2 cut(s) 292, 319
MboII GAAGA 3 cut(s) 6, 37, 430
MhlI GDGCHC 3 cut(s) 174, 397, 454
MluCI AATT 3 cut(s) 77, 344, 376
MlyI GAGTC 2 cut(s) 21, 432
MnlI CCTC 2 cut(s) 49, 371
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 159, 413
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 280, 458
NlaIII CATG 2 cut(s) 418, 474
PaeR7I CTCGAG 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 5 cut(s) 100, 103, 205, 208, 276
PleI GAGTC 2 cut(s) 21, 432
PpsI GAGTC 2 cut(s) 21, 432
Psp124BI GAGCTC 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 20
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 2 cut(s) 159, 413
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 5 cut(s) 99, 102, 204, 207, 275
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 2 cut(s) 21, 432
SduI GDGCHC 3 cut(s) 174, 397, 454
SetI ASST 7 cut(s) 100, 106, 126, 154, 268, 454, 463
SfaNI GCATC 2 cut(s) 292, 319
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 413
SmlI CTYRAG 2 cut(s) 136, 362
SmoI CTYRAG 2 cut(s) 136, 362
Sse9I AATT 3 cut(s) 77, 344, 376
SsiI CCGC 5 cut(s) 18, 57, 203, 206, 332
SstI GAGCTC 1 cut(s) 454
TaqI TCGA 2 cut(s) 126, 137
TasI AATT 3 cut(s) 77, 344, 376
TatI WGTACW 1 cut(s) 254
TauI GCSGC 2 cut(s) 206, 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 3 cut(s) 98, 101, 274
TspDTI ATGAA 4 cut(s) 90, 232, 397, 431
TspGWI ACGGA 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 379
XapI RAATTY 3 cut(s) 77, 344, 376
XhoI CTCGAG 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.