Rh4BG033900

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009048
Physical Location & Seq
Forward (+)
6156960 .. 6160140
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG033900.1

Sequence Viewer

Length: 459 bp
ATGCTTAACCGTTATGATGAGATAGTTTTCACAAGTGTTGCTATATATAGGTTCAATGAATTTGGTTCGGATGGGGGAGCTGCAGCTAAGTATTTGAGACCAAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCCAAGCAAGTTGAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTCGGGGGTGTTCTTTTCATCTTTGGAAGTACTCTTGGAGCTGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGGTTAGGAATCAGGATTACTTGCTGCCTCATAATTTTGTTTACGCATTAGATCTATATCTTTGTCTCTATTTCACATTTTCCCCTTATAAAATGAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

152

Amino Acids

17.34

Weight (kDa)

8.59

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 9 - 119 4.9e-40 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
AciI CCGC 3 cut(s) 185, 188, 314
AcsI RAATTY 2 cut(s) 59, 326
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 3 cut(s) 55, 202, 331
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 5 cut(s) 80, 86, 106, 134, 248
AluI AGCT 5 cut(s) 80, 86, 106, 134, 248
Alw26I GTCTC 2 cut(s) 91, 425
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 80, 83, 256, 379
ApoI RAATTY 2 cut(s) 59, 326
AsiGI ACCGGT 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 156
BbvI GCAGC 4 cut(s) 67, 95, 243, 366
BccI CCATC 2 cut(s) 65, 132
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 91, 425
BfmI CTRYAG 1 cut(s) 81
BglII AGATCT 1 cut(s) 406
BisI GCNGC 6 cut(s) 81, 84, 186, 189, 257, 380
BlsI GCNGC 6 cut(s) 82, 85, 187, 190, 258, 381
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 2 cut(s) 274, 301
BpuEI CTTGAG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 354
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse118I RCCGGY 1 cut(s) 140
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 76
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 1 cut(s) 273
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 4 cut(s) 67, 95, 243, 366
BshFI GGCC 1 cut(s) 272
BshTI ACCGGT 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 2 cut(s) 91, 425
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 91
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 3 cut(s) 185, 188, 314
BspANI GGCC 1 cut(s) 272
BspMAI CTGCAG 1 cut(s) 85
BspTNI GGTCTC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 76
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 2 cut(s) 91, 425
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 67, 95, 243, 366
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 1 cut(s) 272
BtsCI GGATG 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 140
Csp6I GTAC 1 cut(s) 237
CspAI ACCGGT 1 cut(s) 140
CviJI RGCY 7 cut(s) 80, 86, 106, 123, 134, 248, 272
CviKI_1 RGCY 7 cut(s) 80, 86, 106, 123, 134, 248, 272
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 87
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 354
Fnu4HI GCNGC 6 cut(s) 81, 84, 186, 189, 257, 380
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 6 cut(s) 81, 84, 186, 189, 257, 380
GluI GCNGC 6 cut(s) 81, 84, 186, 189, 257, 380
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 141
HinfI GANTC 1 cut(s) 364
HpaII CCGG 1 cut(s) 141
Hpy166II GTNNAC 2 cut(s) 351, 397
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 118, 199, 368
Hpy8I GTNNAC 2 cut(s) 351, 397
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 4 cut(s) 83, 148, 265, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 1 cut(s) 87
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 77, 245
LpnPI CCDG 5 cut(s) 154, 254, 341, 353, 368
Lsp1109I GCAGC 4 cut(s) 67, 95, 243, 366
LweI GCATC 2 cut(s) 274, 301
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 59, 326, 388
MnlI CCTC 2 cut(s) 353, 393
MseI TTAA 2 cut(s) 6, 171
MslI CAYNNNNRTG 1 cut(s) 141
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 1 cut(s) 262
NdeII GATC 1 cut(s) 406
PaeR7I CTCGAG 1 cut(s) 118
PfeI GAWTC 1 cut(s) 364
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 6 cut(s) 82, 85, 187, 190, 258, 381
PsiI TTATAA 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 2 cut(s) 6, 171
SatI GCNGC 6 cut(s) 81, 84, 186, 189, 257, 380
Sau3AI GATC 1 cut(s) 406
ScaI AGTACT 1 cut(s) 238
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 1 cut(s) 156
SetI ASST 7 cut(s) 53, 82, 88, 108, 136, 250, 360
SfaNI GCATC 2 cut(s) 274, 301
SfcI CTRYAG 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 118
SlaI CTCGAG 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 118, 344
SmoI CTYRAG 2 cut(s) 118, 344
Sse9I AATT 3 cut(s) 59, 326, 388
SsiI CCGC 3 cut(s) 185, 188, 314
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 108, 119
TasI AATT 3 cut(s) 59, 326, 388
TatI WGTACW 1 cut(s) 236
TauI GCSGC 2 cut(s) 188, 191
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 6, 171
Tru9I TTAA 2 cut(s) 6, 171
TseI GCWGC 4 cut(s) 80, 83, 256, 379
TspDTI ATGAA 2 cut(s) 72, 214
XapI RAATTY 2 cut(s) 59, 326
XhoI CTCGAG 1 cut(s) 118
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.