Rh4CG041900

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009048
Physical Location & Seq
Forward (+)
8526275 .. 8527829
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG041900.1

Sequence Viewer

Length: 474 bp
ATGGCCTTCGTTTCCTCCGTTGGGAGACTCCTTTTCGCCTCCGTCTTCCTTCTCTCCGCTTGGCAAGAGTTCAATGAATTTGGTTCGGATGGGGGAGCTGCAGCTAAGTATTTGAGACCGAAGCTCGATGTTTTCTCGAGCCATCTGTTAGCTCATACCGGTGTGCAAGTGCCCAAGCAAGTTGAGATTAAGCATTTAGTGGCGGCGGCGATTGTCTTGAAGGGTGTCGGGGGTGTTCTTTTCATCTTTGGAAGTACTCTTGGAGCTGTTCTGCTGCTTCTGCATCAGGCCATTGCAACTCCTATATTGTATGACTTCTACAACTATGATGCGGAGAAAAAAGAATTCAATCAACTTTTCCTCAAGTTTACCCAGAATTTGGCATTGTTTGGGGCTCTGCTTTTTTTCATAAGCATGAAGATATCAATGCCAAAGAGACTCAGGAAAAGAACTCCCAAAGCTAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

157

Amino Acids

17.38

Weight (kDa)

9.94

Isoelectric Point (pI)

26.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 1.4e-59 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 4 cut(s) 57, 203, 206, 332
AcsI RAATTY 3 cut(s) 77, 344, 376
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 2 cut(s) 21, 379
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 220, 349
AluBI AGCT 6 cut(s) 98, 104, 124, 152, 266, 461
AluI AGCT 6 cut(s) 98, 104, 124, 152, 266, 461
Alw26I GTCTC 3 cut(s) 19, 109, 430
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 288
ApeKI GCWGC 3 cut(s) 98, 101, 274
ApoI RAATTY 3 cut(s) 77, 344, 376
AsiGI ACCGGT 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 37
BbvI GCAGC 3 cut(s) 85, 113, 261
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 3 cut(s) 19, 109, 430
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 5 cut(s) 99, 102, 204, 207, 275
BlsI GCNGC 5 cut(s) 100, 103, 205, 208, 276
BmcAI AGTACT 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 136
BmsI GCATC 2 cut(s) 292, 319
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 379
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 291
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 21, 379
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 454
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 3 cut(s) 85, 113, 261
BshFI GGCC 2 cut(s) 5, 290
BshTI ACCGGT 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 21, 379
BsmAI GTCTC 3 cut(s) 19, 109, 430
BsnI GGCC 2 cut(s) 5, 290
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 174, 397
BspACI CCGC 4 cut(s) 57, 203, 206, 332
BspANI GGCC 2 cut(s) 5, 290
BspCNI CTCAG 1 cut(s) 453
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 105, 440
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 3 cut(s) 19, 109, 430
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 85, 113, 261
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 5, 290
BtsCI GGATG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 255
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 415, 471
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 105, 440
Eco24I GRGCYC 1 cut(s) 397
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 344
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 397
FaeI CATG 2 cut(s) 418, 474
FaiI YATR 7 cut(s) 156, 305, 312, 327, 410, 416, 472
FatI CATG 2 cut(s) 414, 470
Fnu4HI GCNGC 5 cut(s) 99, 102, 204, 207, 275
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 5 cut(s) 99, 102, 204, 207, 275
GluI GCNGC 5 cut(s) 99, 102, 204, 207, 275
HaeIII GGCC 2 cut(s) 5, 290
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 418, 474
HinfI GANTC 2 cut(s) 27, 438
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 136, 217, 442
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 16, 59, 214
HpyCH4V TGCA 4 cut(s) 101, 166, 283, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 2 cut(s) 105, 440
Hsp92II CATG 2 cut(s) 418, 474
LmnI GCTCC 2 cut(s) 95, 263
LpnPI CCDG 4 cut(s) 172, 272, 386, 427
Lsp1109I GCAGC 3 cut(s) 85, 113, 261
LweI GCATC 2 cut(s) 292, 319
MboII GAAGA 2 cut(s) 37, 430
MhlI GDGCHC 2 cut(s) 174, 397
MluCI AATT 3 cut(s) 77, 344, 376
MlyI GAGTC 2 cut(s) 21, 432
MnlI CCTC 3 cut(s) 25, 49, 371
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 159, 413
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 280
NlaIII CATG 2 cut(s) 418, 474
PaeR7I CTCGAG 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 5 cut(s) 100, 103, 205, 208, 276
PleI GAGTC 2 cut(s) 21, 432
PpsI GAGTC 2 cut(s) 21, 432
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 2 cut(s) 159, 413
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 5 cut(s) 99, 102, 204, 207, 275
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 2 cut(s) 21, 432
SduI GDGCHC 2 cut(s) 174, 397
SetI ASST 6 cut(s) 100, 106, 126, 154, 268, 463
SfaNI GCATC 2 cut(s) 292, 319
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 413
SmlI CTYRAG 2 cut(s) 136, 362
SmoI CTYRAG 2 cut(s) 136, 362
Sse9I AATT 3 cut(s) 77, 344, 376
SsiI CCGC 4 cut(s) 57, 203, 206, 332
TaqI TCGA 2 cut(s) 126, 137
TaqII GACCGA 1 cut(s) 133
TasI AATT 3 cut(s) 77, 344, 376
TatI WGTACW 1 cut(s) 254
TauI GCSGC 2 cut(s) 206, 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 3 cut(s) 98, 101, 274
TspDTI ATGAA 4 cut(s) 90, 232, 397, 431
TspGWI ACGGA 2 cut(s) 7, 31
Van91I CCANNNNNTGG 1 cut(s) 379
XapI RAATTY 3 cut(s) 77, 344, 376
XhoI CTCGAG 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.