AT4G25850

protein)-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004160
Physical Location & Seq
Forward (+)
13142164 .. 13146966
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25850.3

Sequence Viewer

Length: 1152 bp
ATGGCAGAAGAAGAAATGAGAAAGCACCTTGTGCTGGCCAAACCTTTTTCGTTGGAAGATGAAAAAGATTCTGAACACACTGCTTCGAATGTCATCCGCAAGATCCTCAGCCTCTTTAAGACCGTACGCCCAGGATCTGATCTCACCAATTTTCAGCTGCCACCTCAGCTGAACCTACCAAGATCACAACTCCAATGTTACGGCGAGATGGTCTACAGCTTCGTCGGTCAGGATCTGCTTGGGGAATGCAGCCGCCGTGATCTTCCCATTGAACGGCTCAAATCAGTGGTGACGTGGAACATCTCCACACTCCGTCCGGTGGTCTTTGGCATGTCTCCGTACAACTCCGTTCTCGGCGAGACTCACCACGTATCGAACGGTCACATCAACGTCATCGCCGAACAAGTAGTGCATCATCCTCCGGTTTCCGCTCTTCATGCGACTCACGAACAAGAAAATATCGACGTGACATGGTGTCAATATTTCACTCCTAAATTTCGTGGTACTCACGTGGACGTTGAGGTAAAGGGTAAAAGGGTAATGAAGCTTCTGAATCATATAGAGACTTATGAGATGGACCAACCGAGACTAATAATGAGATTCTTACCGGCGCCGGGAGCTTACTGGGCCGGAAAAGTGAAAATAAAGTGTCCGGAGACTGATCTCGAAGCTGAGTTACATTTGATCTCCGATTCATTCATTGAAAGATTCAGAGGCAACAACAACAGATCAATAAAAGGAAAGATCTTTGAATCTTCCTCTGGTAATCAGCTCTACAACATTTTTGGCCATTGGGACAGAACGGTAATGGCGAAAAATATAAAGACTGGTGAGCTCGAGGTCATTTACAATGCCAAGGAGAATATCACAGGACTCAAACCTCCAACAGTCAAGAACCTGGAGGAGGTAACGGAGAGCGAGTCGACAATGGTGTGGAGTGAAGTAAGTGAAGGAATACTGAAGAAAGATTGGGAAAGAGCAAGAGAAGCTAAGATAGTTGTGGAGGAGAAGCAGAGAGCGTCTTTAAAACAGAGAGAGGCTTCTGGTGAGTCATGGGTTCCCAAACATTTCTTAGTGGTCAAAGACGGTAAAGACTGGGATTGTTCACCGCTACAGCCTACGGTTCCTCCGGCGCCACTCGTCATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

383

Amino Acids

43.67

Weight (kDa)

6.55

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 38 - 266 6.5e-38 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 610, 1132
AccBSI CCGCTC 1 cut(s) 431
AccI GTMKAC 2 cut(s) 213, 923
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 4 cut(s) 97, 253, 429, 1109
AclWI GGATC 3 cut(s) 97, 142, 240
AcoI YGGCCR 2 cut(s) 36, 787
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 980
AcvI CACGTG 1 cut(s) 511
AcyI GRCGYC 2 cut(s) 611, 1133
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 3 cut(s) 126, 341, 505
AfiI CCNNNNNNNGG 5 cut(s) 34, 273, 319, 614, 904
AgsI TTSAA 3 cut(s) 272, 704, 752
AhdI GACNNNNNGTC 1 cut(s) 474
AjiI CACGTC 2 cut(s) 294, 466
AjnI CCWGG 2 cut(s) 130, 897
AluBI AGCT 9 cut(s) 157, 169, 219, 547, 620, 671, 772, 835, 989
AluI AGCT 9 cut(s) 157, 169, 219, 547, 620, 671, 772, 835, 989
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 5 cut(s) 339, 353, 557, 580, 650
AlwI GGATC 3 cut(s) 97, 142, 240
AlwNI CAGNNNCTG 2 cut(s) 137, 235
Ama87I CYCGRG 1 cut(s) 836
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 3 cut(s) 36, 627, 787
ApeKI GCWGC 2 cut(s) 157, 249
ApoI RAATTY 1 cut(s) 494
AspLEI GCGC 2 cut(s) 613, 1135
AspS9I GGNCC 2 cut(s) 577, 627
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 6 cut(s) 136, 301, 356, 842, 1058, 1098
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 836
AvaII GGWCC 1 cut(s) 577
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BalI TGGCCA 2 cut(s) 38, 789
BanI GGYRCC 2 cut(s) 610, 1132
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 4 cut(s) 531, 563, 660, 692
BbrPI CACGTG 1 cut(s) 511
Bbv12I GWGCWC 1 cut(s) 837
BbvCI CCTCAGC 2 cut(s) 107, 165
BbvI GCAGC 2 cut(s) 144, 261
BccI CCATC 2 cut(s) 202, 568
BceAI ACGGC 3 cut(s) 217, 240, 290
BciT130I CCWGG 2 cut(s) 132, 899
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 5 cut(s) 339, 353, 557, 580, 650
BfmI CTRYAG 2 cut(s) 214, 1112
BfoI RGCGCY 2 cut(s) 614, 1136
BglII AGATCT 1 cut(s) 744
BisI GCNGC 3 cut(s) 158, 250, 253
BlsI GCNGC 3 cut(s) 159, 251, 254
Bme1390I CCNGG 3 cut(s) 132, 615, 899
Bme18I GGWCC 1 cut(s) 577
BmeRI GACNNNNNGTC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 836
BmgBI CACGTC 2 cut(s) 294, 466
BmgT120I GGNCC 2 cut(s) 577, 627
BmiI GGNNCC 4 cut(s) 612, 1059, 1125, 1134
BmrFI CCNGG 3 cut(s) 132, 615, 899
BmrI ACTGGG 2 cut(s) 634, 1105
BmsI GCATC 1 cut(s) 421
BmuI ACTGGG 2 cut(s) 634, 1105
BpmI CTGGAG 1 cut(s) 920
Bpu10I CCTNAGC 2 cut(s) 107, 165
Bpu14I TTCGAA 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 615
BsaAI YACGTR 2 cut(s) 370, 511
BsaHI GRCGYC 2 cut(s) 611, 1133
BsaJI CCNNGG 2 cut(s) 130, 855
BsaWI WCCGGW 3 cut(s) 316, 421, 652
BsaXI ACNNNNNCTCC 4 cut(s) 905, 935, 1111, 1141
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 273, 319, 614, 904
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 3 cut(s) 629, 832, 1100
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 2 cut(s) 132, 899
BseDI CCNNGG 2 cut(s) 130, 855
BseGI GGATG 2 cut(s) 93, 415
BseLI CCNNNNNNNGG 5 cut(s) 34, 273, 319, 614, 904
BseMII CTCAG 3 cut(s) 121, 179, 663
BseNI ACTGG 3 cut(s) 629, 832, 1100
BseRI GAGGAG 2 cut(s) 917, 1019
BseXI GCAGC 2 cut(s) 144, 261
BshFI GGCC 3 cut(s) 38, 629, 789
BshNI GGYRCC 2 cut(s) 610, 1132
BsiHKAI GWGCWC 1 cut(s) 837
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 7 cut(s) 317, 422, 608, 614, 630, 653, 1130
BsiWI CGTACG 1 cut(s) 124
BslFI GGGAC 1 cut(s) 809
BslI CCNNNNNNNGG 5 cut(s) 34, 273, 319, 614, 904
BsmAI GTCTC 5 cut(s) 339, 353, 557, 580, 650
BsmFI GGGAC 1 cut(s) 809
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 3 cut(s) 38, 629, 789
BsoBI CYCGRG 1 cut(s) 836
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 837
Bsp13I TCCGGA 1 cut(s) 652
BspACI CCGC 4 cut(s) 97, 253, 429, 1109
BspANI GGCC 3 cut(s) 38, 629, 789
BspCNI CTCAG 3 cut(s) 120, 178, 664
BspEI TCCGGA 1 cut(s) 652
BspLI GGNNCC 4 cut(s) 612, 1059, 1125, 1134
BspPI GGATC 3 cut(s) 97, 142, 240
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 1 cut(s) 86
BspT107I GGYRCC 2 cut(s) 610, 1132
BsrBI CCGCTC 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 3 cut(s) 629, 832, 1100
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 130, 855
BssNI GRCGYC 2 cut(s) 611, 1133
BssT1I CCWWGG 1 cut(s) 855
Bst2UI CCWGG 2 cut(s) 132, 899
Bst4CI ACNGT 6 cut(s) 124, 380, 805, 889, 1088, 1123
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 611, 1133
BstAPI GCANNNNNTGC 1 cut(s) 31
BstBAI YACGTR 2 cut(s) 370, 511
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 5 cut(s) 107, 165, 672, 990, 1072
BstENI CCTNNNNNAGG 1 cut(s) 902
BstF5I GGATG 2 cut(s) 93, 415
BstH2I RGCGCY 2 cut(s) 614, 1136
BstHHI GCGC 2 cut(s) 613, 1135
BstMAI GTCTC 5 cut(s) 339, 353, 557, 580, 650
BstMWI GCNNNNNNNGC 6 cut(s) 31, 166, 437, 617, 626, 986
BstNI CCWGG 2 cut(s) 132, 899
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 130, 613, 897
BstSFI CTRYAG 2 cut(s) 214, 1112
BstV1I GCAGC 2 cut(s) 144, 261
BstX2I RGATCY 4 cut(s) 102, 134, 232, 744
BstYI RGATCY 4 cut(s) 102, 134, 232, 744
BsuRI GGCC 3 cut(s) 38, 629, 789
BtgZI GCGATG 1 cut(s) 379
BtrI CACGTC 2 cut(s) 294, 466
BtsCI GGATG 2 cut(s) 93, 415
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 78, 291
Cac8I GCNNGC 1 cut(s) 36
CaiI CAGNNNCTG 2 cut(s) 137, 235
CfoI GCGC 2 cut(s) 613, 1135
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 2 cut(s) 577, 627
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 3 cut(s) 125, 340, 504
CviAII CATG 4 cut(s) 331, 437, 471, 1053
CviQI GTAC 3 cut(s) 125, 340, 504
DdeI CTNAG 5 cut(s) 107, 165, 672, 990, 1072
DinI GGCGCC 2 cut(s) 612, 1134
DraI TTTAAA 1 cut(s) 1026
DraIII CACNNNGTG 1 cut(s) 31
DriI GACNNNNNGTC 1 cut(s) 474
EaeI YGGCCR 2 cut(s) 36, 787
Eam1104I CTCTTC 1 cut(s) 438
Eam1105I GACNNNNNGTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 855
Eco24I GRGCYC 1 cut(s) 837
Eco47I GGWCC 1 cut(s) 577
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 980
Eco72I CACGTG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 902
EcoRII CCWGG 2 cut(s) 130, 897
EcoT14I CCWWGG 1 cut(s) 855
EcoT38I GRGCYC 1 cut(s) 837
EgeI GGCGCC 2 cut(s) 612, 1134
EheI GGCGCC 2 cut(s) 612, 1134
ErhI CCWWGG 1 cut(s) 855
FaeI CATG 4 cut(s) 334, 440, 474, 1056
FaiI YATR 9 cut(s) 332, 438, 472, 558, 560, 570, 821, 1054, 1150
FaqI GGGAC 1 cut(s) 809
FatI CATG 4 cut(s) 330, 436, 470, 1052
FblI GTMKAC 2 cut(s) 213, 923
Fnu4HI GCNGC 3 cut(s) 158, 250, 253
FokI GGATG 2 cut(s) 80, 402
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 3 cut(s) 158, 250, 253
GlaI GCGC 2 cut(s) 612, 1134
GluI GCNGC 3 cut(s) 158, 250, 253
GsuI CTGGAG 1 cut(s) 920
HaeII RGCGCY 2 cut(s) 614, 1136
HaeIII GGCC 3 cut(s) 38, 629, 789
HapII CCGG 7 cut(s) 317, 422, 608, 614, 630, 653, 1130
HgaI GACGC 1 cut(s) 1008
HhaI GCGC 2 cut(s) 613, 1135
Hin1I GRCGYC 2 cut(s) 611, 1133
Hin1II CATG 4 cut(s) 334, 440, 474, 1056
Hin6I GCGC 2 cut(s) 611, 1133
HinP1I GCGC 2 cut(s) 611, 1133
HincII GTYRAC 1 cut(s) 924
HindII GTYRAC 1 cut(s) 924
HindIII AAGCTT 1 cut(s) 545
HpaII CCGG 7 cut(s) 317, 422, 608, 614, 630, 653, 1130
HphI GGTGA 6 cut(s) 136, 301, 356, 842, 1058, 1098
Hpy166II GTNNAC 4 cut(s) 214, 514, 924, 1106
Hpy188I TCNGA 5 cut(s) 73, 139, 552, 691, 713
Hpy188III TCNNGA 5 cut(s) 230, 446, 653, 665, 892
Hpy8I GTNNAC 4 cut(s) 214, 514, 924, 1106
Hpy99I CGWCG 2 cut(s) 227, 467
HpyAV CCTTC 1 cut(s) 944
HpyCH4III ACNGT 6 cut(s) 124, 380, 805, 889, 1088, 1123
HpyCH4IV ACGT 6 cut(s) 293, 369, 390, 465, 510, 516
HpyCH4V TGCA 2 cut(s) 249, 412
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 166, 437, 617, 626, 986
HpyF3I CTNAG 5 cut(s) 107, 165, 672, 990, 1072
HpySE526I ACGT 6 cut(s) 293, 369, 390, 465, 510, 516
Hsp92I GRCGYC 2 cut(s) 611, 1133
Hsp92II CATG 4 cut(s) 334, 440, 474, 1056
HspAI GCGC 2 cut(s) 611, 1133
KasI GGCGCC 2 cut(s) 610, 1132
Kpn2I TCCGGA 1 cut(s) 652
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 2 cut(s) 144, 261
LweI GCATC 1 cut(s) 421
MaeII ACGT 6 cut(s) 293, 369, 390, 465, 510, 516
MaeIII GTNAC 6 cut(s) 197, 289, 380, 466, 675, 907
MbiI CCGCTC 1 cut(s) 431
MboII GAAGA 7 cut(s) 20, 23, 68, 254, 425, 747, 973
MflI RGATCY 4 cut(s) 102, 134, 232, 744
MhlI GDGCHC 1 cut(s) 837
MlsI TGGCCA 2 cut(s) 38, 789
MluCI AATT 2 cut(s) 148, 494
MluNI TGGCCA 2 cut(s) 38, 789
Mly113I GGCGCC 2 cut(s) 611, 1133
MlyI GAGTC 5 cut(s) 355, 436, 867, 929, 1058
MmeI TCCRAC 2 cut(s) 33, 908
Mox20I TGGCCA 2 cut(s) 38, 789
MroI TCCGGA 1 cut(s) 652
MscI TGGCCA 2 cut(s) 38, 789
MseI TTAA 2 cut(s) 117, 1025
Msp20I TGGCCA 2 cut(s) 38, 789
MspA1I CMGCKG 2 cut(s) 157, 169
MspI CCGG 7 cut(s) 317, 422, 608, 614, 630, 653, 1130
MspR9I CCNGG 3 cut(s) 132, 615, 899
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 2 cut(s) 132, 899
MwoI GCNNNNNNNGC 6 cut(s) 31, 166, 437, 617, 626, 986
NarI GGCGCC 2 cut(s) 611, 1133
NciI CCSGG 1 cut(s) 615
NlaIII CATG 4 cut(s) 334, 440, 474, 1056
NlaIV GGNNCC 4 cut(s) 612, 1059, 1125, 1134
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 3 cut(s) 289, 380, 466
NspI RCATGY 1 cut(s) 334
NspV TTCGAA 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 2 cut(s) 396, 809
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 6 cut(s) 68, 553, 600, 692, 708, 752
Pfl23II CGTACG 1 cut(s) 124
PkrI GCNGC 3 cut(s) 159, 251, 254
PleI GAGTC 5 cut(s) 355, 436, 867, 928, 1057
PluTI GGCGCC 2 cut(s) 614, 1136
PmaCI CACGTG 1 cut(s) 511
PmlI CACGTG 1 cut(s) 511
PpsI GAGTC 5 cut(s) 355, 436, 867, 928, 1057
Ppu21I YACGTR 2 cut(s) 370, 511
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 2 cut(s) 130, 897
PspCI CACGTG 1 cut(s) 511
PspGI CCWGG 2 cut(s) 130, 897
PspLI CGTACG 1 cut(s) 124
PspN4I GGNNCC 4 cut(s) 612, 1059, 1125, 1134
PspPI GGNCC 2 cut(s) 577, 627
PspXI VCTCGAGB 1 cut(s) 836
PstNI CAGNNNCTG 2 cut(s) 137, 235
PsuI RGATCY 4 cut(s) 102, 134, 232, 744
PvuII CAGCTG 2 cut(s) 157, 169
RsaI GTAC 3 cut(s) 126, 341, 505
RsaNI GTAC 3 cut(s) 125, 340, 504
SacI GAGCTC 1 cut(s) 837
SalI GTCGAC 1 cut(s) 922
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 2 cut(s) 117, 1025
SatI GCNGC 3 cut(s) 158, 250, 253
Sau96I GGNCC 2 cut(s) 577, 627
SchI GAGTC 5 cut(s) 355, 436, 867, 929, 1058
ScrFI CCNGG 3 cut(s) 132, 615, 899
SduI GDGCHC 1 cut(s) 837
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 2 cut(s) 214, 1112
SfoI GGCGCC 2 cut(s) 612, 1134
Sfr274I CTCGAG 1 cut(s) 836
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 1 cut(s) 577
SlaI CTCGAG 1 cut(s) 836
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 2 cut(s) 148, 494
SsiI CCGC 4 cut(s) 97, 253, 429, 1109
SspDI GGCGCC 2 cut(s) 610, 1132
SspI AATATT 1 cut(s) 482
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 3 cut(s) 130, 613, 897
StyI CCWWGG 1 cut(s) 855
TaaI ACNGT 6 cut(s) 124, 380, 805, 889, 1088, 1123
TaiI ACGT 6 cut(s) 296, 372, 393, 468, 513, 519
TaqI TCGA 6 cut(s) 86, 374, 462, 666, 837, 923
TaqII GACCGA 1 cut(s) 215
TasI AATT 2 cut(s) 148, 494
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 6 cut(s) 68, 553, 600, 692, 708, 752
Tru1I TTAA 2 cut(s) 117, 1025
Tru9I TTAA 2 cut(s) 117, 1025
TscAI CASTG 2 cut(s) 85, 291
TseFI GTSAC 3 cut(s) 289, 380, 466
TseI GCWGC 2 cut(s) 157, 249
Tsp45I GTSAC 3 cut(s) 289, 380, 466
TspDTI ATGAA 5 cut(s) 75, 425, 557, 684, 688
TspGWI ACGGA 4 cut(s) 302, 327, 337, 926
TspRI CASTG 2 cut(s) 85, 291
VpaK11BI GGWCC 1 cut(s) 577
XagI CCTNNNNNAGG 1 cut(s) 902
XapI RAATTY 1 cut(s) 494
XceI RCATGY 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 836
XmiI GTMKAC 2 cut(s) 213, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.