MD16G1249900.v1.1

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004160
Physical Location & Seq
Forward (+)
27991534 .. 28002127
10594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1249900.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGTGAAGGAGGAAGAGAATACCGGAGTTATTCTTTTGACAAAGCCGTTGTCACTGGAAGGGGATTCCGATGTTGATTACAGAGCTCCTAATCTACTTCAACGCGTCGGAAGCCTGTTTAAAAACGTACGGCCTGGATCTGATCTCACAAGCTTCCAGCTGTCACCGCTGTTTAACATACCAAAGTCACACCTCCAATGTTTCGGTGAATCAGTGTACTGCGTAGGTAATGATTTGTTAGGAAGGTGCAACAAAGCAGAAACCCCTCATGACAGGTTCACAGCTGTTGTATCGTGGAGCATTTCTACTTTGCGTCCAGTGATCTTCGGAATTGCTCCGTACAACCCCATTCTTGGAGAAACCCATCATGTTACAAGAGGGAATCTCAATGTTCTACTTGAGCAGGTTTCACATCATCCACCAGTTACCGCTCTTCATGCAACTGATGAAAAAGAAAACCTTGAAATGATATGGTGTCAACATCCTACTCCCAAATTCTATGGTACTTCAGTGGAAACTGAGGTGCGTGGAAAACGGCAGTTGAAGCTCCTGAATCACGGAGAAACTTATGAAATGAACTCGCCGAAACTCTTAATCAAGTTCCTCCCATTGCCTTGGGTTGATTGGGTTGGTGAAAATAGAATCCGGTGTCATGAGACGGGCCTTGAAGCTGAGTTATATTATGGAAGTAATTCATGGATTAGGGGAAATCCTAGAACAATTAAGGGAAAGATCTTTGACTCAACAACATATGAACTTCTTTATGAGATACATGGTCGGTGGGATAGAACTGTCAAAATGAAGGACGTTAGTAGCGGGAAAGAAACCGTTATATACAATGCAAATGAAGTCATTTCAGAACTGAAAACTCCGATTGTTACGGATTTGAAGGGGGTGTGGCCAAGTGAATCAGCTGCGGTCTGGGGACTGCTGAGCCAAGCAATTATTGGGAAGGACTGGGGCAAAGCAAGAGAAGCGAAGAAAGCAGTGGAAGAAAAACACAGGGAGCTCCTGCGAGAGAGAGAATCGAGAGGTGAAACTTGGATTCCCAAACATTTTACAGTCACTCATAGCAAAGAAGGTTGTGGGTGGGACTGCTCACCCATCCAGCAGCTGGTCCCGCTGGCTCCTATTTCTGTACCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

383

Amino Acids

43.22

Weight (kDa)

6.71

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 40 - 280 1.3e-45 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 1114
AccBSI CCGCTC 1 cut(s) 431
AccII CGCG 1 cut(s) 105
AciI CCGC 5 cut(s) 167, 429, 816, 917, 1121
AclWI GGATC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 899
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 5 cut(s) 129, 218, 341, 505, 1140
AfiI CCNNNNNNNGG 2 cut(s) 353, 1114
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 5 cut(s) 101, 464, 544, 668, 889
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 9 cut(s) 86, 153, 160, 284, 547, 671, 914, 1009, 1114
AluI AGCT 9 cut(s) 86, 153, 160, 284, 547, 671, 914, 1009, 1114
Alw21I GWGCWC 2 cut(s) 88, 1011
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 145
AlwNI CAGNNNCTG 1 cut(s) 1114
AoxI GGCC 3 cut(s) 131, 661, 899
ApeKI GCWGC 2 cut(s) 914, 1111
ApoI RAATTY 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
Asp700I GAANNNNTTC 1 cut(s) 691
AspS9I GGNCC 2 cut(s) 661, 1117
AsuHPI GGTGA 6 cut(s) 16, 156, 218, 644, 1046, 1092
AvaII GGWCC 1 cut(s) 1117
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BalI TGGCCA 1 cut(s) 901
BanII GRGCYC 2 cut(s) 88, 1011
Bbv12I GWGCWC 2 cut(s) 88, 1011
BbvI GCAGC 2 cut(s) 901, 1123
BccI CCATC 2 cut(s) 372, 1112
BceAI ACGGC 3 cut(s) 31, 146, 551
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 394
BglII AGATCT 1 cut(s) 732
BisI GCNGC 2 cut(s) 915, 1112
BlpI GCTNAGC 1 cut(s) 932
BlsI GCNGC 2 cut(s) 916, 1113
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 1117
BmgT120I GGNCC 2 cut(s) 661, 1117
BmiI GGNNCC 2 cut(s) 1119, 1128
BmrFI CCNGG 1 cut(s) 135
BmrI ACTGGG 1 cut(s) 967
BmuI ACTGGG 1 cut(s) 967
BplI GAGNNNNNCTC 2 cut(s) 369, 401
Bpu1102I GCTNAGC 1 cut(s) 932
BpuEI CTTGAG 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 2 cut(s) 23, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1114
Bse1I ACTGG 4 cut(s) 60, 317, 422, 962
Bse3DI GCAATG 1 cut(s) 608
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 3 cut(s) 415, 481, 1104
BseLI CCNNNNNNNGG 2 cut(s) 353, 1114
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 3 cut(s) 510, 663, 923
BseNI ACTGG 4 cut(s) 60, 317, 422, 962
BseXI GCAGC 2 cut(s) 901, 1123
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 133, 663, 901
BsiHKAI GWGCWC 2 cut(s) 88, 1011
BsiSI CCGG 2 cut(s) 24, 646
BsiWI CGTACG 1 cut(s) 127
BslFI GGGAC 3 cut(s) 939, 1103, 1106
BslI CCNNNNNNNGG 2 cut(s) 353, 1114
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 3 cut(s) 939, 1103, 1106
BsnI GGCC 3 cut(s) 133, 663, 901
Bsp1286I GDGCHC 2 cut(s) 88, 1011
Bsp143I GATC 4 cut(s) 137, 142, 321, 732
Bsp1720I GCTNAGC 1 cut(s) 932
BspACI CCGC 5 cut(s) 167, 429, 816, 917, 1121
BspANI GGCC 3 cut(s) 133, 663, 901
BspCNI CTCAG 3 cut(s) 511, 664, 924
BspFNI CGCG 1 cut(s) 105
BspHI TCATGA 2 cut(s) 268, 652
BspLI GGNNCC 2 cut(s) 1119, 1128
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 1 cut(s) 145
BspQI GCTCTTC 1 cut(s) 438
BsrBI CCGCTC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 4 cut(s) 60, 317, 422, 962
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 4 cut(s) 137, 142, 321, 732
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 3 cut(s) 793, 829, 1063
Bst6I CTCTTC 2 cut(s) 9, 438
BstC8I GCNNGC 1 cut(s) 1125
BstDEI CTNAG 3 cut(s) 519, 672, 932
BstF5I GGATG 3 cut(s) 415, 481, 1104
BstFNI CGCG 1 cut(s) 105
BstKTI GATC 4 cut(s) 140, 145, 324, 735
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 4 cut(s) 137, 142, 321, 732
BstMWI GCNNNNNNNGC 7 cut(s) 111, 166, 437, 544, 974, 983, 1120
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 901, 1123
BstX2I RGATCY 2 cut(s) 137, 732
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 2 cut(s) 137, 732
BsuRI GGCC 3 cut(s) 133, 663, 901
BtsCI GGATG 3 cut(s) 415, 481, 1104
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 5 cut(s) 53, 219, 324, 516, 993
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 1125
CaiI CAGNNNCTG 1 cut(s) 1114
CciI TCATGA 2 cut(s) 268, 652
Cfr13I GGNCC 2 cut(s) 661, 1117
CseI GACGC 2 cut(s) 94, 302
Csp6I GTAC 5 cut(s) 128, 217, 340, 504, 1139
CviAII CATG 6 cut(s) 269, 368, 437, 653, 696, 773
CviQI GTAC 5 cut(s) 128, 217, 340, 504, 1139
DdeI CTNAG 3 cut(s) 519, 672, 932
DpnI GATC 4 cut(s) 139, 144, 323, 734
DpnII GATC 4 cut(s) 137, 142, 321, 732
DraI TTTAAA 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 899
Eam1104I CTCTTC 2 cut(s) 9, 438
EarI CTCTTC 2 cut(s) 9, 438
Ecl136II GAGCTC 2 cut(s) 86, 1009
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 2 cut(s) 88, 1011
Eco47I GGWCC 1 cut(s) 1117
Eco53kI GAGCTC 2 cut(s) 86, 1009
Eco57I CTGAAG 1 cut(s) 492
EcoICRI GAGCTC 2 cut(s) 86, 1009
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 614
EcoT38I GRGCYC 2 cut(s) 88, 1011
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 6 cut(s) 272, 371, 440, 656, 699, 776
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 3 cut(s) 939, 1103, 1106
FatI CATG 6 cut(s) 268, 367, 436, 652, 695, 772
FauI CCCGC 2 cut(s) 809, 1128
FauNDI CATATG 1 cut(s) 751
Fnu4HI GCNGC 2 cut(s) 915, 1112
FokI GGATG 3 cut(s) 402, 468, 1091
FriOI GRGCYC 2 cut(s) 88, 1011
Fsp4HI GCNGC 2 cut(s) 915, 1112
FspBI CTAG 1 cut(s) 714
GluI GCNGC 2 cut(s) 915, 1112
HaeIII GGCC 3 cut(s) 133, 663, 901
HapII CCGG 2 cut(s) 24, 646
HgaI GACGC 2 cut(s) 94, 302
Hin1II CATG 6 cut(s) 272, 371, 440, 656, 699, 776
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 9 cut(s) 65, 209, 382, 553, 642, 740, 908, 1025, 1045
HpaII CCGG 2 cut(s) 24, 646
HphI GGTGA 6 cut(s) 16, 156, 218, 644, 1046, 1092
Hpy166II GTNNAC 3 cut(s) 217, 279, 479
Hpy188I TCNGA 6 cut(s) 70, 110, 142, 329, 859, 873
Hpy188III TCNNGA 4 cut(s) 269, 550, 653, 1029
Hpy8I GTNNAC 3 cut(s) 217, 279, 479
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 6 cut(s) 53, 237, 796, 883, 946, 1073
HpyCH4III ACNGT 3 cut(s) 793, 829, 1063
HpyCH4IV ACGT 2 cut(s) 126, 807
HpyCH4V TGCA 3 cut(s) 249, 440, 842
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 166, 437, 544, 974, 983, 1120
HpyF3I CTNAG 3 cut(s) 519, 672, 932
HpySE526I ACGT 2 cut(s) 126, 807
Hsp92II CATG 6 cut(s) 272, 371, 440, 656, 699, 776
Kzo9I GATC 4 cut(s) 137, 142, 321, 732
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 7 cut(s) 91, 297, 340, 552, 1006, 1014, 1132
Lsp1109I GCAGC 2 cut(s) 901, 1123
MaeI CTAG 1 cut(s) 714
MaeII ACGT 2 cut(s) 126, 807
MaeIII GTNAC 7 cut(s) 51, 162, 186, 370, 424, 877, 1063
MalI GATC 4 cut(s) 139, 144, 323, 734
MbiI CCGCTC 1 cut(s) 431
MboI GATC 4 cut(s) 137, 142, 321, 732
MboII GAAGA 5 cut(s) 26, 316, 425, 991, 1004
MflI RGATCY 2 cut(s) 137, 732
MhlI GDGCHC 2 cut(s) 88, 1011
MlsI TGGCCA 1 cut(s) 901
MluCI AATT 5 cut(s) 330, 494, 691, 720, 942
MluI ACGCGT 1 cut(s) 103
MluNI TGGCCA 1 cut(s) 901
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 7 cut(s) 4, 203, 276, 371, 514, 614, 1025
Mox20I TGGCCA 1 cut(s) 901
MroXI GAANNNNTTC 1 cut(s) 691
MscI TGGCCA 1 cut(s) 901
MseI TTAA 4 cut(s) 120, 174, 593, 723
Msp20I TGGCCA 1 cut(s) 901
MspA1I CMGCKG 6 cut(s) 160, 169, 284, 914, 1114, 1123
MspI CCGG 2 cut(s) 24, 646
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 7 cut(s) 111, 166, 437, 544, 974, 983, 1120
NdeI CATATG 1 cut(s) 751
NdeII GATC 4 cut(s) 137, 142, 321, 732
NlaIII CATG 6 cut(s) 272, 371, 440, 656, 699, 776
NlaIV GGNNCC 2 cut(s) 1119, 1128
NmuCI GTSAC 4 cut(s) 51, 162, 186, 1063
PagI TCATGA 2 cut(s) 268, 652
PciSI GCTCTTC 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 8 cut(s) 65, 209, 382, 553, 642, 908, 1025, 1045
Pfl23II CGTACG 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 1114
PkrI GCNGC 2 cut(s) 916, 1113
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
Psp124BI GAGCTC 2 cut(s) 88, 1011
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspLI CGTACG 1 cut(s) 127
PspN4I GGNNCC 2 cut(s) 1119, 1128
PspPI GGNCC 2 cut(s) 661, 1117
PstNI CAGNNNCTG 1 cut(s) 1114
PsuI RGATCY 2 cut(s) 137, 732
PvuII CAGCTG 4 cut(s) 160, 284, 914, 1114
RsaI GTAC 5 cut(s) 129, 218, 341, 505, 1140
RsaNI GTAC 5 cut(s) 128, 217, 340, 504, 1139
SacI GAGCTC 2 cut(s) 88, 1011
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 4 cut(s) 120, 174, 593, 723
SatI GCNGC 2 cut(s) 915, 1112
Sau3AI GATC 4 cut(s) 137, 142, 321, 732
Sau96I GGNCC 2 cut(s) 661, 1117
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 2 cut(s) 88, 1011
SinI GGWCC 1 cut(s) 1117
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 5 cut(s) 330, 494, 691, 720, 942
SsiI CCGC 5 cut(s) 167, 429, 816, 917, 1121
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 2 cut(s) 88, 1011
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 3 cut(s) 793, 829, 1063
TaiI ACGT 2 cut(s) 129, 810
TaqI TCGA 1 cut(s) 1028
TasI AATT 5 cut(s) 330, 494, 691, 720, 942
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 8 cut(s) 65, 209, 382, 553, 642, 908, 1025, 1045
Tru1I TTAA 4 cut(s) 120, 174, 593, 723
Tru9I TTAA 4 cut(s) 120, 174, 593, 723
TscAI CASTG 5 cut(s) 60, 219, 324, 516, 993
TseFI GTSAC 4 cut(s) 51, 162, 186, 1063
TseI GCWGC 2 cut(s) 914, 1111
Tsp45I GTSAC 4 cut(s) 51, 162, 186, 1063
TspDTI ATGAA 8 cut(s) 425, 462, 585, 590, 684, 768, 815, 861
TspGWI ACGGA 3 cut(s) 327, 573, 896
TspRI CASTG 5 cut(s) 60, 219, 324, 516, 993
Van91I CCANNNNNTGG 1 cut(s) 1114
VpaK11BI GGWCC 1 cut(s) 1117
XapI RAATTY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 944, 1111
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.