Rorug04G0019100

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004160
Physical Location & Seq
Forward (+)
2828663 .. 2832018
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0019100.1

Sequence Viewer

Length: 2937 bp
ATGGGGCAAAGCGATTCAAAGCAAAATGTGGCTTCTGCGATTAATGCAGAAGCCAGTTCTTCATCCTCTTCCCTCCCACGTTCTTCTACTGATCACCAAAGTCATTACACATACGAGGTCTTCCTGAGTTTTCGAGGCGAGGATACGCGCTTTAGTTTTACAGATCATTTGTATACTGCTCTGTATCGGGGGGGAATTGAGACCTTCAGAGATGATGGGCTCAGAAGAGGAGAAGAAATATCATCGGCTCTTCTCAAAGCAATTGAGGAATCGAGAGTTTCAATCATCGTCTTCTCTCAAAACTATGCTGCCTCAAGGTGGTGCTTAGATGAACTGGTCAAGATACTTGACTGCAGAAAATCCAAAGGGCAGGAGGTTAGAGCGGTGTTCTACAAGGTGGATCCGTCAGATGTACGACACCAAAGAGGTGCATTCGGTGACGCATTTGCTACACTTGATCAATGCAAATACAAGGATAGCATGGACAAATGGAAGGCAGCTCTCAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCCAGTACGAGTCTAAATTTATCAATGACATTGTTGGGGAGTTGTCTGCCCGAGTGGTAAACCCTTCATGTGAGTTGCAAGTAGCTGCACATCCTATTGGAATAGAGTCTTGTAGACAAGATGTCAACAAACTTTTACACACCAGGGAAAATATTGTTCGCATGGTAGGTATATGGGGGCCTGGTGGAATAGGAAAGACCACAATTGCAAAAGATGTGTTTAATTCTATTCGCCAAAACTTCGAAGGTAGTTGTTTCTTGGCAGATGTTAGATCTAATTCAAATGGTCTAGCCCAACTACAAGAGACACTTTTGTCTAATATTTTAAACTCAACTTTGAAGGTGAGCAGTGTTGATGAAGGAGTGAGTTTAATAAAGACAAGGATGCGACATAAAAAAATTCTCTTAATCCTTGATGACGTGTCTCATTCCAGGCAATTACAGAACTTAGTTCCATCCCCTGATTGTTTTGGGCTGGGCAGCCGAATTCTCATAACAACAAGAGATAAACGTTTGCTAATTGCTCATCAAGTCGATGAAGTATACGAGGTCAAGATGTTAGATTATGATCAAGCTTTGGAGTTGTTTAGCTTAAATGCTTTCAAAAGAAATGGACCTCCAAGGGATTATCTGGAACTTGCACGACGTGCTATACGTTATGCCCAGGGCCTTCCATTAGCTTTGATTGTTTTAGGGTCTCATCTATTTTGTAGAAGCAGAGAGGAGTGGGAAGCTACATTAGGTAGTTGTAAAGAAGATCCCCACAGAGAGATAAGAGACGTGCTCAAAATAAGTTATGATGCTCTGGGAGTAGATTTAAAAGGATATTTTCTTGATATCGCTTGTTTCTTTAAGGGTAAGCCTGTAGACAATGTGAAACCAATACTAGAAGCTTGCTACGACCTCAAATCAGTGAATGGTATTGCACAACTCCAAGAAAAGGCCTTAATAAGAATTGATAGATTGCACTGGAGAAGTGGAATAGACAAAGGTTGTATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTATATCAAGAGTCACCTGGTGAGCCCGGGGAACGCAGCAGAGTGTGGAGCAAAGAAGATGTCGACCACATTCTAACAAATAATACAGGCACAGATAAAATTATAGGCATCCAGTTCCGGGAACATTCTAAGATATCTTTGAATGCTAAAAGCTTCTCAGGGATGAAGAATCTTAGATATATTTCTTTGAGCAGAATGATGAAAAATGAATGCTTTTCTGGAGACATTGACTATCTCTCCAACCAGTTGAGGTGGTTTGATTGGTCTGAATGTCCGTTGCAATCTTTTCCATCTGATTTTCATGCAAACAAACTTGTAAAGCTCAATATTCCTTGTAGCTGCAGAATCACACGACTATGGGAGGGACGTAAGAATTTTTCAAGGCTGACATGTATGAATTTAAGCGGTTGTGATTCCCTAACAAAACTCCCAGACTTTAGTGGAATCCCCAACTTGAAAGAGTTGAATCTATATGAATGTCGCAATTTAGTTGCGCTTCCTGATTCCGTTGGATTCCTTCGTAACCTTGTCATCTTGAATGTTTCCTGCTGCTCTAACCTTATTATGTTTCCAAGAAAAATCAACTGGAAATATGTAGAAACTATTTCTATCCGCTCTCGCAAGCTTGAGGAATTTTCAGAAGTTGGGAAAGAGATGGGTTCCTTGAGAATTTTGGATCTATCAGGCACTTACATTAAAGAATTGGATAAGTCAATTGGAAATCTCATTTGGCTCGAAGAGTTGAGGCTGCTGGATTGTCATAATCTTACAACTCTGCCGCATAGCATTTATGAGTTGCGCAATCTACAAGTTCTTGATGCGAATAATTGCTCAAAACTTGCTGCATTTCCAGAAATTCCAATAAAGATGGATTTCTTGAGACAACTTTATTTAGAAGGCAGTGACATCAGAGAATTGGATGAGTCAATTGGAAAGCTCATTGGGCTTGAAGGGTTGTTCCTCAGTGATTGCAAAAATCTTACAACTCTACCGTGCAGCATTTATGGATTGCAAAATTTAAAGAGGTTTGAGCTTACTAACTGCTCGAAACTTGTCAGATTTCCAACAAATGTCGATGGTTGCTCACTATCACTTCCCAAGCTATATGCGTTCAGTATCGGAGGATGCAGTTTATCAGATTGTGATTTCCTCATGACTCTTGATTGCTGGGAAATATTAGAGTACCTTGATCTGTCAGAAAACAATTTTGTTAGTGTTCCTGCGTGCATCACCAAATTTGTCAACTTGCGGGCGCTTTTCTTGGAGGGTTGCAAGAGACTCCGAGAAATTCCAGAGCTTCCACCACTTTTCCTAGACTTAAGTGGTTGTGAATCACTGGAGAGATTCTCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

978

Amino Acids

110.94

Weight (kDa)

6.37

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 38 - 199 4.1e-52 TIR domain
TIR_2 PF13676 40 - 135 9.1e-13 TIR domain
NB-ARC PF00931 224 - 384 6.7e-23 NB-ARC domain
WHD_ROQ1 PF23282 455 - 532 4.3e-10 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 746 - 807 2.6e-08 Leucine-rich repeat region
LRR_13 PF23286 794 - 976 1.4e-24 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 856
Acc16I TGCGCA 1 cut(s) 2381
AccBSI CCGCTC 2 cut(s) 383, 2196
AccI GTMKAC 5 cut(s) 173, 658, 1086, 1410, 1644
AccII CGCG 1 cut(s) 148
AciI CCGC 5 cut(s) 383, 1986, 2194, 2360, 2830
AclI AACGTT 1 cut(s) 1054
AclWI GGATC 4 cut(s) 395, 408, 1295, 2265
AcoI YGGCCR 1 cut(s) 544
AcuI CTGAAG 1 cut(s) 190
AdeI CACNNNGTG 2 cut(s) 1190, 1601
AfaI GTAC 3 cut(s) 414, 551, 2765
AfiI CCNNNNNNNGG 3 cut(s) 318, 1483, 1699
AflII CTTAAG 1 cut(s) 2899
AflIII ACRYGT 2 cut(s) 963, 1970
AhdI GACNNNNNGTC 1 cut(s) 665
AjiI CACGTC 3 cut(s) 964, 1190, 1324
AjnI CCWGG 5 cut(s) 686, 724, 974, 1206, 1597
AjuI GAANNNNNNNTTGG 2 cut(s) 2293, 2325
Alw21I GWGCWC 1 cut(s) 1329
Alw26I GTCTC 8 cut(s) 194, 842, 972, 1245, 1314, 1797, 2455, 2851
AlwI GGATC 4 cut(s) 395, 408, 1295, 2265
Ama87I CYCGRG 2 cut(s) 594, 1607
AoxI GGCC 5 cut(s) 530, 544, 722, 1210, 1485
ArsI GACNNNNNNTTYG 2 cut(s) 1626, 1658
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 4 cut(s) 150, 2077, 2382, 2836
AspS9I GGNCC 3 cut(s) 722, 1157, 1210
AsuC2I CCSGG 3 cut(s) 1608, 1609, 1700
AsuHPI GGTGA 6 cut(s) 86, 449, 898, 1587, 1613, 2803
AsuII TTCGAA 1 cut(s) 786
AvaI CYCGRG 2 cut(s) 594, 1607
AvaII GGWCC 1 cut(s) 1157
BalI TGGCCA 1 cut(s) 546
BamHI GGATCC 1 cut(s) 400
BanII GRGCYC 2 cut(s) 222, 1608
BbsI GAAGAC 2 cut(s) 112, 283
Bbv12I GWGCWC 1 cut(s) 1329
BccI CCATC 8 cut(s) 209, 522, 536, 1006, 1879, 2230, 2443, 2651
BciT130I CCWGG 5 cut(s) 688, 726, 976, 1208, 1599
BciVI GTATCC 1 cut(s) 136
BclI TGATCA 3 cut(s) 91, 457, 1111
BcnI CCSGG 3 cut(s) 1608, 1609, 1700
BcoDI GTCTC 8 cut(s) 194, 842, 972, 1245, 1314, 1797, 2455, 2851
BfaI CTAG 3 cut(s) 833, 1430, 2894
BfmI CTRYAG 3 cut(s) 352, 1407, 1921
BfoI RGCGCY 1 cut(s) 2837
BfrI CTTAAG 1 cut(s) 2899
BfuI GTATCC 1 cut(s) 136
BglII AGATCT 1 cut(s) 815
Bme1390I CCNGG 8 cut(s) 688, 726, 976, 1208, 1599, 1608, 1609, 1700
Bme18I GGWCC 1 cut(s) 1157
BmeRI GACNNNNNGTC 1 cut(s) 665
BmeT110I CYCGRG 2 cut(s) 594, 1607
BmgBI CACGTC 3 cut(s) 964, 1190, 1324
BmgT120I GGNCC 3 cut(s) 722, 1157, 1210
BmiI GGNNCC 3 cut(s) 402, 723, 2242
BmrFI CCNGG 8 cut(s) 688, 726, 976, 1208, 1599, 1608, 1609, 1700
BmsI GCATC 7 cut(s) 918, 1333, 1536, 1698, 2389, 2696, 2817
BpiI GAAGAC 2 cut(s) 112, 283
BplI GAGNNNNNCTC 3 cut(s) 1311, 1343, 2912
BpmI CTGGAG 2 cut(s) 1534, 1821
Bpu14I TTCGAA 1 cut(s) 786
BpuEI CTTGAG 5 cut(s) 298, 488, 2228, 2266, 2479
BpuMI CCSGG 3 cut(s) 1608, 1609, 1700
BsaBI GATNNNNATC 1 cut(s) 1110
BsaI GGTCTC 2 cut(s) 194, 1245
BsaJI CCNNGG 6 cut(s) 687, 1163, 1206, 1207, 1607, 1608
BsaXI ACNNNNNCTCC 2 cut(s) 1802, 1832
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 1483, 1699
Bse1I ACTGG 8 cut(s) 54, 339, 547, 1517, 1693, 1825, 2171, 2922
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 5 cut(s) 688, 726, 976, 1208, 1599
BseDI CCNNGG 6 cut(s) 687, 1163, 1206, 1207, 1607, 1608
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 3 cut(s) 318, 1483, 1699
BseMII CTCAG 4 cut(s) 116, 235, 1752, 2557
BseNI ACTGG 8 cut(s) 54, 339, 547, 1517, 1693, 1825, 2171, 2922
BseRI GAGGAG 2 cut(s) 243, 1280
BseYI CCCAGC 2 cut(s) 1018, 2748
BsgI GTGCAG 2 cut(s) 615, 2596
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 5 cut(s) 532, 546, 724, 1212, 1487
BsiHKAI GWGCWC 1 cut(s) 1329
BsiHKCI CYCGRG 2 cut(s) 594, 1607
BsiSI CCGG 2 cut(s) 1608, 1699
BslFI GGGAC 1 cut(s) 1959
BslI CCNNNNNNNGG 3 cut(s) 318, 1483, 1699
BsmAI GTCTC 8 cut(s) 194, 842, 972, 1245, 1314, 1797, 2455, 2851
BsmBI CGTCTC 1 cut(s) 1314
BsmFI GGGAC 1 cut(s) 1959
BsmI GAATGC 3 cut(s) 431, 1729, 1796
BsnI GGCC 5 cut(s) 532, 546, 724, 1212, 1487
Bso31I GGTCTC 2 cut(s) 194, 1245
BsoBI CYCGRG 2 cut(s) 594, 1607
Bsp119I TTCGAA 1 cut(s) 786
Bsp1286I GDGCHC 3 cut(s) 222, 1329, 1608
Bsp143I GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
BspACI CCGC 5 cut(s) 383, 1986, 2194, 2360, 2830
BspANI GGCC 5 cut(s) 532, 546, 724, 1212, 1487
BspCNI CTCAG 4 cut(s) 117, 234, 1751, 2556
BspFNI CGCG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 2733
BspLI GGNNCC 3 cut(s) 402, 723, 2242
BspMAI CTGCAG 2 cut(s) 356, 1925
BspPI GGATC 4 cut(s) 395, 408, 1295, 2265
BspQI GCTCTTC 1 cut(s) 255
BspT104I TTCGAA 1 cut(s) 786
BspTI CTTAAG 1 cut(s) 2899
BspTNI GGTCTC 2 cut(s) 194, 1245
BsrBI CCGCTC 2 cut(s) 383, 2196
BsrI ACTGG 8 cut(s) 54, 339, 547, 1517, 1693, 1825, 2171, 2922
BssECI CCNNGG 6 cut(s) 687, 1163, 1206, 1207, 1607, 1608
BssMI GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
BssNAI GTATAC 2 cut(s) 174, 1087
BssT1I CCWWGG 1 cut(s) 1163
Bst1107I GTATAC 2 cut(s) 174, 1087
Bst2UI CCWGG 5 cut(s) 688, 726, 976, 1208, 1599
Bst4CI ACNGT 1 cut(s) 2574
Bst6I CTCTTC 4 cut(s) 73, 220, 255, 2313
BstAFI CTTAAG 1 cut(s) 2899
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 786
BstC8I GCNNGC 4 cut(s) 1438, 2204, 2806, 2832
BstDEI CTNAG 8 cut(s) 125, 221, 325, 991, 1710, 1738, 1754, 2543
BstFNI CGCG 1 cut(s) 148
BstH2I RGCGCY 1 cut(s) 2837
BstHHI GCGC 4 cut(s) 150, 2077, 2382, 2836
BstKTI GATC 9 cut(s) 94, 166, 403, 460, 818, 1114, 1303, 2260, 2773
BstMAI GTCTC 8 cut(s) 194, 842, 972, 1245, 1314, 1797, 2455, 2851
BstMBI GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
BstMWI GCNNNNNNNGC 3 cut(s) 44, 1190, 2524
BstNI CCWGG 5 cut(s) 688, 726, 976, 1208, 1599
BstNSI RCATGY 1 cut(s) 1974
BstSCI CCNGG 8 cut(s) 686, 724, 974, 1206, 1597, 1606, 1607, 1698
BstSFI CTRYAG 3 cut(s) 352, 1407, 1921
BstUI CGCG 1 cut(s) 148
BstV2I GAAGAC 2 cut(s) 112, 283
BstX2I RGATCY 4 cut(s) 400, 815, 1300, 2257
BstYI RGATCY 4 cut(s) 400, 815, 1300, 2257
BstZ17I GTATAC 2 cut(s) 174, 1087
BsuI GTATCC 1 cut(s) 136
BsuRI GGCC 5 cut(s) 532, 546, 724, 1212, 1487
BtrI CACGTC 3 cut(s) 964, 1190, 1324
BtsI GCAGTG 2 cut(s) 898, 2488
BtsIMutI CAGTG 6 cut(s) 898, 1461, 1510, 2488, 2551, 2915
Cac8I GCNNGC 4 cut(s) 1438, 2204, 2806, 2832
CciI TCATGA 1 cut(s) 2733
CfoI GCGC 4 cut(s) 150, 2077, 2382, 2836
Cfr13I GGNCC 3 cut(s) 722, 1157, 1210
Cfr9I CCCGGG 1 cut(s) 1607
CseI GACGC 1 cut(s) 449
CsiI ACCWGGT 1 cut(s) 1597
Csp6I GTAC 3 cut(s) 413, 550, 2764
CviAII CATG 7 cut(s) 481, 612, 706, 1550, 1883, 1971, 2734
CviQI GTAC 3 cut(s) 413, 550, 2764
DdeI CTNAG 8 cut(s) 125, 221, 325, 991, 1710, 1738, 1754, 2543
DpnI GATC 9 cut(s) 93, 165, 402, 459, 817, 1113, 1302, 2259, 2772
DpnII GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
DraI TTTAAA 3 cut(s) 870, 1362, 2601
DraIII CACNNNGTG 2 cut(s) 1190, 1601
DrdI GACNNNNNNGTC 1 cut(s) 856
DriI GACNNNNNGTC 1 cut(s) 665
DseDI GACNNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 4 cut(s) 73, 220, 255, 2313
Eam1105I GACNNNNNGTC 1 cut(s) 665
EarI CTCTTC 4 cut(s) 73, 220, 255, 2313
Eco130I CCWWGG 1 cut(s) 1163
Eco147I AGGCCT 1 cut(s) 1487
Eco24I GRGCYC 2 cut(s) 222, 1608
Eco31I GGTCTC 2 cut(s) 194, 1245
Eco32I GATATC 2 cut(s) 1381, 1716
Eco47I GGWCC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 190
Eco88I CYCGRG 2 cut(s) 594, 1607
EcoO109I RGGNCCY 2 cut(s) 722, 1210
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 5 cut(s) 686, 724, 974, 1206, 1597
EcoRV GATATC 2 cut(s) 1381, 1716
EcoT14I CCWWGG 1 cut(s) 1163
EcoT22I ATGCAT 1 cut(s) 1551
EcoT38I GRGCYC 2 cut(s) 222, 1608
ErhI CCWWGG 1 cut(s) 1163
Esp3I CGTCTC 1 cut(s) 1314
FaeI CATG 7 cut(s) 484, 615, 709, 1553, 1886, 1974, 2737
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 1 cut(s) 1959
FatI CATG 7 cut(s) 480, 611, 705, 1549, 1882, 1970, 2733
FauI CCCGC 1 cut(s) 2823
FbaI TGATCA 3 cut(s) 91, 457, 1111
FblI GTMKAC 5 cut(s) 173, 658, 1086, 1410, 1644
FriOI GRGCYC 2 cut(s) 222, 1608
FspBI CTAG 3 cut(s) 833, 1430, 2894
FspI TGCGCA 1 cut(s) 2381
GlaI GCGC 4 cut(s) 149, 2076, 2381, 2835
GsaI CCCAGC 2 cut(s) 1022, 2752
GsuI CTGGAG 2 cut(s) 1534, 1821
HaeII RGCGCY 1 cut(s) 2837
HaeIII GGCC 5 cut(s) 532, 546, 724, 1212, 1487
HapII CCGG 2 cut(s) 1608, 1699
HgaI GACGC 1 cut(s) 449
HhaI GCGC 4 cut(s) 150, 2077, 2382, 2836
Hin1II CATG 7 cut(s) 484, 615, 709, 1553, 1886, 1974, 2737
Hin6I GCGC 4 cut(s) 148, 2075, 2380, 2834
HinP1I GCGC 4 cut(s) 148, 2075, 2380, 2834
HincII GTYRAC 3 cut(s) 670, 1645, 2824
HindII GTYRAC 3 cut(s) 670, 1645, 2824
HindIII AAGCTT 4 cut(s) 1116, 1434, 1732, 2204
HpaII CCGG 2 cut(s) 1608, 1699
HphI GGTGA 6 cut(s) 86, 449, 898, 1587, 1613, 2803
Hpy166II GTNNAC 8 cut(s) 174, 604, 659, 670, 1087, 1411, 1645, 2824
Hpy8I GTNNAC 8 cut(s) 174, 604, 659, 670, 1087, 1411, 1645, 2824
Hpy99I CGWCG 1 cut(s) 1191
HpyCH4III ACNGT 1 cut(s) 2574
HpyCH4IV ACGT 7 cut(s) 79, 963, 1054, 1189, 1198, 1323, 1948
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 1190, 2524
HpyF3I CTNAG 8 cut(s) 125, 221, 325, 991, 1710, 1738, 1754, 2543
HpySE526I ACGT 7 cut(s) 79, 963, 1054, 1189, 1198, 1323, 1948
Hsp92II CATG 7 cut(s) 484, 615, 709, 1553, 1886, 1974, 2737
HspAI GCGC 4 cut(s) 148, 2075, 2380, 2834
Ksp22I TGATCA 3 cut(s) 91, 457, 1111
Kzo9I GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 1629
LweI GCATC 7 cut(s) 918, 1333, 1536, 1698, 2389, 2696, 2817
MabI ACCWGGT 1 cut(s) 1597
MaeI CTAG 3 cut(s) 833, 1430, 2894
MaeII ACGT 7 cut(s) 79, 963, 1054, 1189, 1198, 1323, 1948
MaeIII GTNAC 4 cut(s) 437, 1593, 2102, 2483
MalI GATC 9 cut(s) 93, 165, 402, 459, 817, 1113, 1302, 2259, 2772
MbiI CCGCTC 2 cut(s) 383, 2196
MboI GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
MfeI CAATTG 4 cut(s) 261, 747, 2295, 2508
MflI RGATCY 4 cut(s) 400, 815, 1300, 2257
MhlI GDGCHC 3 cut(s) 222, 1329, 1608
MlsI TGGCCA 1 cut(s) 546
MluNI TGGCCA 1 cut(s) 546
MlyI GAGTC 6 cut(s) 563, 659, 1601, 2513, 2731, 2853
MmeI TCCRAC 3 cut(s) 1845, 2071, 2669
Mox20I TGGCCA 1 cut(s) 546
Mph1103I ATGCAT 1 cut(s) 1551
MscI TGGCCA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 1936
Msp20I TGGCCA 1 cut(s) 546
MspCI CTTAAG 1 cut(s) 2899
MspI CCGG 2 cut(s) 1608, 1699
MspR9I CCNGG 8 cut(s) 688, 726, 976, 1208, 1599, 1608, 1609, 1700
MunI CAATTG 4 cut(s) 261, 747, 2295, 2508
Mva1269I GAATGC 3 cut(s) 431, 1729, 1796
MvaI CCWGG 5 cut(s) 688, 726, 976, 1208, 1599
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 3 cut(s) 44, 1190, 2524
NciI CCSGG 3 cut(s) 1608, 1609, 1700
NdeII GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
NlaIII CATG 7 cut(s) 484, 615, 709, 1553, 1886, 1974, 2737
NlaIV GGNNCC 3 cut(s) 402, 723, 2242
NmuCI GTSAC 3 cut(s) 437, 1593, 2483
NsbI TGCGCA 1 cut(s) 2381
NsiI ATGCAT 1 cut(s) 1551
NspI RCATGY 1 cut(s) 1974
NspV TTCGAA 1 cut(s) 786
PagI TCATGA 1 cut(s) 2733
PasI CCCWGGG 1 cut(s) 1207
PceI AGGCCT 1 cut(s) 1487
PciI ACATGT 1 cut(s) 1970
PciSI GCTCTTC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 1195
PctI GAATGC 3 cut(s) 431, 1729, 1796
PfoI TCCNGGA 1 cut(s) 1698
PleI GAGTC 6 cut(s) 562, 658, 1600, 2512, 2731, 2853
PpsI GAGTC 6 cut(s) 562, 658, 1600, 2512, 2731, 2853
PscI ACATGT 1 cut(s) 1970
PshBI ATTAAT 1 cut(s) 42
Psp1406I AACGTT 1 cut(s) 1054
Psp6I CCWGG 5 cut(s) 686, 724, 974, 1206, 1597
PspFI CCCAGC 2 cut(s) 1018, 2748
PspGI CCWGG 5 cut(s) 686, 724, 974, 1206, 1597
PspN4I GGNNCC 3 cut(s) 402, 723, 2242
PspPI GGNCC 3 cut(s) 722, 1157, 1210
PstI CTGCAG 2 cut(s) 356, 1925
PsuI RGATCY 4 cut(s) 400, 815, 1300, 2257
RsaI GTAC 3 cut(s) 414, 551, 2765
RsaNI GTAC 3 cut(s) 413, 550, 2764
RseI CAYNNNNRTG 1 cut(s) 1936
SalI GTCGAC 1 cut(s) 1643
SapI GCTCTTC 1 cut(s) 255
Sau3AI GATC 9 cut(s) 91, 163, 400, 457, 815, 1111, 1300, 2257, 2770
Sau96I GGNCC 3 cut(s) 722, 1157, 1210
SchI GAGTC 6 cut(s) 563, 659, 1601, 2513, 2731, 2853
ScrFI CCNGG 8 cut(s) 688, 726, 976, 1208, 1599, 1608, 1609, 1700
SduI GDGCHC 3 cut(s) 222, 1329, 1608
SexAI ACCWGGT 1 cut(s) 1597
SfaNI GCATC 7 cut(s) 918, 1333, 1536, 1698, 2389, 2696, 2817
SfcI CTRYAG 3 cut(s) 352, 1407, 1921
SfuI TTCGAA 1 cut(s) 786
SinI GGWCC 1 cut(s) 1157
SmaI CCCGGG 1 cut(s) 1609
SmiMI CAYNNNNRTG 1 cut(s) 1936
SmlI CTYRAG 6 cut(s) 313, 503, 2207, 2245, 2458, 2899
SmoI CTYRAG 6 cut(s) 313, 503, 2207, 2245, 2458, 2899
SseBI AGGCCT 1 cut(s) 1487
SsiI CCGC 5 cut(s) 383, 1986, 2194, 2360, 2830
SspI AATATT 4 cut(s) 697, 865, 1909, 2757
SspMI CTAG 3 cut(s) 833, 1430, 2894
StuI AGGCCT 1 cut(s) 1487
StyD4I CCNGG 8 cut(s) 686, 724, 974, 1206, 1597, 1606, 1607, 1698
StyI CCWWGG 1 cut(s) 1163
TaaI ACNGT 1 cut(s) 2574
TaiI ACGT 7 cut(s) 82, 966, 1057, 1192, 1201, 1326, 1951
TaqI TCGA 9 cut(s) 133, 272, 786, 1077, 1644, 2316, 2627, 2655, 2930
TauI GCSGC 1 cut(s) 2362
TscAI CASTG 6 cut(s) 898, 1461, 1517, 2488, 2551, 2922
TseFI GTSAC 3 cut(s) 437, 1593, 2483
Tsp45I GTSAC 3 cut(s) 437, 1593, 2483
TspGWI ACGGA 3 cut(s) 393, 1845, 2077
TspMI CCCGGG 1 cut(s) 1607
TspRI CASTG 6 cut(s) 898, 1461, 1517, 2488, 2551, 2922
Vha464I CTTAAG 1 cut(s) 2899
VpaK11BI GGWCC 1 cut(s) 1157
VspI ATTAAT 1 cut(s) 42
XceI RCATGY 1 cut(s) 1974
XmaI CCCGGG 1 cut(s) 1607
XmiI GTMKAC 5 cut(s) 173, 658, 1086, 1410, 1644
XspI CTAG 3 cut(s) 833, 1430, 2894
Zsp2I ATGCAT 1 cut(s) 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.