AT4G25860

protein)-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004160
Physical Location & Seq
Forward (+)
13147289 .. 13149606
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25860.1

Sequence Viewer

Length: 1161 bp
ATGGCAGAAGAAGAGAAAATGAGAAGGCAACTTGTGCTGGCCAAACCTTTTTCTCTGGAAGATGAGAAAGATTCTGAGCTCACAGCTTCAAGTGTCATCCGCAAGATCCTCAGTCTCATTAAGACCGTACGTCCAGGATCTGATCTCACCAATTTTCAGCTGCCACCTCAGCTGATCCTACCAAGATCACGACTCCAATGTTATGGGGAGATGATCTACAGCTTCGGCGGTCAGGATCTACTGGGCGAATGCAGCCGCCGTAACATTCCCATCGAACGGCTCAAATCGGTGGTGACGTGGAACATCTCCACACTCCGTCCCGTGGTCGTTGGCATGTCTCCGTACAACCCCATTCTCGGCGAGACTCATCACGTCTCCAACGGCTACATCAACGTCCTCACCGAACAAGTAATGCATCATCCTCCGGTGTCCGCACTTCATGCGACTCACGAACAAGAAAATATCGACGTCACATGGTGTCAATATTTCACTCCCAAATTTCGTGGTGCGTACGTGGATGTTGAGGTAAATGGTAAAAGGATAATGAAGCTTCTGCATCATAAAGAGACTTATGAGATGGACCAACCGAGACTAATTATGAAATTCTTACCGGCGCCGGGAGCTCATTGGGCCGGAAAAGTCAAGATAAAGTGTCCTGAGACTGATCTCGAAGCTGAGTTACATTTGATCTCCGACTCATTCATTGAAAGATTCAGAGGCAACAACAACAGATCAATCAAAGGAAAGATCTTTGAATCTTCCTCTGGTAATCAGCTCTACAACATCTTTGGCCATTGGGACAGAACGGTAATGGCAAAAAATCTGAAGACTGGTGGGCTCGAGGTTATCTACAATGCCAATGAGAATATCACAGGACTCAAACCTCCAACTGTCAAGAATCTGCAGGAGGTAATGGAGAGCGAGTCGACAATAGTGTGGAGTGAAGTAAGTGAAGGAATACTGAAGAAAGATTGGGAAAGAGCAAGAGAAGCTAAGATACTTGTGGAGGAGAAGCAGAGAGAGGCTCTGAAACAGAGAGAGGCTTCTGGTGAGTCATGGGTTCCCAAACATTTCTCAGTAGTCAAAGACGGTAAAGACTGGCACTGCTCACCGCTACAGCCCACGGTTCCTCGGGCTCCACTCGTCATCACAGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

386

Amino Acids

44.07

Weight (kDa)

8.15

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 38 - 367 7.2e-46 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 613
AccI GTMKAC 1 cut(s) 926
AciI CCGC 5 cut(s) 100, 228, 256, 432, 1112
AclWI GGATC 4 cut(s) 100, 145, 169, 243
AcoI YGGCCR 2 cut(s) 39, 790
AcsI RAATTY 2 cut(s) 497, 602
AcuI CTGAAG 2 cut(s) 845, 983
AcyI GRCGYC 2 cut(s) 468, 614
AdeI CACNNNGTG 1 cut(s) 477
AfaI GTAC 3 cut(s) 129, 344, 512
AfiI CCNNNNNNNGG 4 cut(s) 276, 322, 356, 617
AgsI TTSAA 3 cut(s) 90, 707, 755
AhdI GACNNNNNGTC 1 cut(s) 129
AjiI CACGTC 2 cut(s) 297, 373
AjnI CCWGG 1 cut(s) 133
Alw21I GWGCWC 2 cut(s) 81, 625
Alw26I GTCTC 7 cut(s) 119, 342, 356, 379, 560, 583, 653
AlwI GGATC 4 cut(s) 100, 145, 169, 243
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 2 cut(s) 839, 1131
AoxI GGCC 3 cut(s) 39, 630, 790
ApeKI GCWGC 2 cut(s) 160, 252
ApoI RAATTY 2 cut(s) 497, 602
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 2 cut(s) 580, 630
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 5 cut(s) 139, 304, 391, 1061, 1101
AvaI CYCGRG 2 cut(s) 839, 1131
AvaII GGWCC 1 cut(s) 580
BalI TGGCCA 2 cut(s) 41, 792
BanI GGYRCC 1 cut(s) 613
BanII GRGCYC 4 cut(s) 81, 625, 840, 1138
BarI GAAGNNNNNNTAC 4 cut(s) 663, 695, 981, 1013
BbsI GAAGAC 1 cut(s) 833
Bbv12I GWGCWC 2 cut(s) 81, 625
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 147, 264
BccI CCATC 2 cut(s) 278, 571
BceAI ACGGC 3 cut(s) 243, 293, 397
BciT130I CCWGG 1 cut(s) 135
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 7 cut(s) 119, 342, 356, 379, 560, 583, 653
BfmI CTRYAG 3 cut(s) 217, 902, 1115
BfoI RGCGCY 1 cut(s) 617
BglII AGATCT 1 cut(s) 747
BisI GCNGC 3 cut(s) 161, 253, 256
BlsI GCNGC 3 cut(s) 162, 254, 257
Bme1390I CCNGG 2 cut(s) 135, 618
Bme18I GGWCC 1 cut(s) 580
BmeRI GACNNNNNGTC 1 cut(s) 129
BmeT110I CYCGRG 2 cut(s) 839, 1131
BmgBI CACGTC 2 cut(s) 297, 373
BmgT120I GGNCC 2 cut(s) 580, 630
BmiI GGNNCC 4 cut(s) 615, 1062, 1128, 1137
BmrFI CCNGG 2 cut(s) 135, 618
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 424, 565
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 833
BplI GAGNNNNNCTC 2 cut(s) 1009, 1041
Bpu10I CCTNAGC 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 514
BsaHI GRCGYC 2 cut(s) 468, 614
BsaJI CCNNGG 3 cut(s) 321, 1122, 1130
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 908, 938
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 322, 356, 617
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 3 cut(s) 246, 835, 1103
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 3 cut(s) 321, 1122, 1130
BseGI GGATG 3 cut(s) 96, 418, 523
BseLI CCNNNNNNNGG 4 cut(s) 276, 322, 356, 617
BseMII CTCAG 6 cut(s) 66, 124, 182, 648, 666, 1089
BseNI ACTGG 3 cut(s) 246, 835, 1103
BseRI GAGGAG 1 cut(s) 1022
BseXI GCAGC 2 cut(s) 147, 264
BshFI GGCC 3 cut(s) 41, 632, 792
BshNI GGYRCC 1 cut(s) 613
BsiHKAI GWGCWC 2 cut(s) 81, 625
BsiHKCI CYCGRG 2 cut(s) 839, 1131
BsiSI CCGG 4 cut(s) 425, 611, 617, 633
BsiWI CGTACG 2 cut(s) 127, 510
BslFI GGGAC 2 cut(s) 303, 812
BslI CCNNNNNNNGG 4 cut(s) 276, 322, 356, 617
BsmAI GTCTC 7 cut(s) 119, 342, 356, 379, 560, 583, 653
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 2 cut(s) 303, 812
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 3 cut(s) 41, 632, 792
BsoBI CYCGRG 2 cut(s) 839, 1131
Bsp1286I GDGCHC 4 cut(s) 81, 625, 840, 1138
BspACI CCGC 5 cut(s) 100, 228, 256, 432, 1112
BspANI GGCC 3 cut(s) 41, 632, 792
BspCNI CTCAG 6 cut(s) 67, 123, 181, 649, 667, 1088
BspLI GGNNCC 4 cut(s) 615, 1062, 1128, 1137
BspMAI CTGCAG 1 cut(s) 906
BspPI GGATC 4 cut(s) 100, 145, 169, 243
BspT107I GGYRCC 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 3 cut(s) 246, 835, 1103
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 3 cut(s) 321, 1122, 1130
BssNI GRCGYC 2 cut(s) 468, 614
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 5 cut(s) 127, 808, 892, 1091, 1126
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 2 cut(s) 468, 614
BstAPI GCANNNNNTGC 2 cut(s) 34, 440
BstBAI YACGTR 1 cut(s) 514
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 7 cut(s) 75, 110, 168, 657, 675, 993, 1075
BstDSI CCRYGG 2 cut(s) 321, 1122
BstF5I GGATG 3 cut(s) 96, 418, 523
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstMAI GTCTC 7 cut(s) 119, 342, 356, 379, 560, 583, 653
BstMWI GCNNNNNNNGC 7 cut(s) 34, 169, 252, 440, 620, 629, 989
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 337
BstSCI CCNGG 2 cut(s) 133, 616
BstSFI CTRYAG 3 cut(s) 217, 902, 1115
BstV1I GCAGC 2 cut(s) 147, 264
BstV2I GAAGAC 1 cut(s) 833
BstX2I RGATCY 4 cut(s) 105, 137, 235, 747
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 4 cut(s) 105, 137, 235, 747
BsuRI GGCC 3 cut(s) 41, 632, 792
BtgI CCRYGG 2 cut(s) 321, 1122
BtrI CACGTC 2 cut(s) 297, 373
BtsCI GGATG 3 cut(s) 96, 418, 523
BtsI GCAGTG 1 cut(s) 1102
BtsIMutI CAGTG 1 cut(s) 1102
Cac8I GCNNGC 1 cut(s) 39
CaiI CAGNNNCTG 1 cut(s) 140
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 2 cut(s) 580, 630
Csp6I GTAC 3 cut(s) 128, 343, 511
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviAII CATG 4 cut(s) 334, 440, 474, 1056
CviQI GTAC 3 cut(s) 128, 343, 511
DdeI CTNAG 7 cut(s) 75, 110, 168, 657, 675, 993, 1075
DinI GGCGCC 1 cut(s) 615
DraIII CACNNNGTG 1 cut(s) 477
DriI GACNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 39, 790
Eam1104I CTCTTC 1 cut(s) 6
Eam1105I GACNNNNNGTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 6
Ecl136II GAGCTC 2 cut(s) 79, 623
Eco24I GRGCYC 4 cut(s) 81, 625, 840, 1138
Eco47I GGWCC 1 cut(s) 580
Eco53kI GAGCTC 2 cut(s) 79, 623
Eco57I CTGAAG 2 cut(s) 845, 983
Eco88I CYCGRG 2 cut(s) 839, 1131
EcoICRI GAGCTC 2 cut(s) 79, 623
EcoRII CCWGG 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 417
EcoT38I GRGCYC 4 cut(s) 81, 625, 840, 1138
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 4 cut(s) 337, 443, 477, 1059
FaiI YATR 8 cut(s) 204, 335, 441, 475, 561, 573, 599, 1057
FaqI GGGAC 2 cut(s) 303, 812
FatI CATG 4 cut(s) 333, 439, 473, 1055
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 3 cut(s) 161, 253, 256
FokI GGATG 3 cut(s) 83, 405, 530
FriOI GRGCYC 4 cut(s) 81, 625, 840, 1138
Fsp4HI GCNGC 3 cut(s) 161, 253, 256
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 161, 253, 256
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 3 cut(s) 41, 632, 792
HapII CCGG 4 cut(s) 425, 611, 617, 633
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 2 cut(s) 468, 614
Hin1II CATG 4 cut(s) 337, 443, 477, 1059
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 1 cut(s) 548
HpaII CCGG 4 cut(s) 425, 611, 617, 633
HphI GGTGA 5 cut(s) 139, 304, 391, 1061, 1101
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 6 cut(s) 76, 142, 694, 716, 825, 1029
Hpy188III TCNNGA 8 cut(s) 56, 189, 233, 449, 643, 656, 668, 895
Hpy8I GTNNAC 1 cut(s) 927
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 18, 947
HpyCH4III ACNGT 5 cut(s) 127, 808, 892, 1091, 1126
HpyCH4IV ACGT 6 cut(s) 130, 296, 372, 393, 468, 513
HpyCH4V TGCA 4 cut(s) 252, 415, 556, 904
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 169, 252, 440, 620, 629, 989
HpyF3I CTNAG 7 cut(s) 75, 110, 168, 657, 675, 993, 1075
HpySE526I ACGT 6 cut(s) 130, 296, 372, 393, 468, 513
Hsp92I GRCGYC 2 cut(s) 468, 614
Hsp92II CATG 4 cut(s) 337, 443, 477, 1059
HspAI GCGC 1 cut(s) 614
KasI GGCGCC 1 cut(s) 613
LmnI GCTCC 2 cut(s) 620, 1141
Lsp1109I GCAGC 2 cut(s) 147, 264
LweI GCATC 2 cut(s) 424, 565
MaeII ACGT 6 cut(s) 130, 296, 372, 393, 468, 513
MaeIII GTNAC 4 cut(s) 260, 292, 469, 678
MboII GAAGA 6 cut(s) 20, 23, 71, 750, 838, 976
MflI RGATCY 4 cut(s) 105, 137, 235, 747
MhlI GDGCHC 4 cut(s) 81, 625, 840, 1138
MlsI TGGCCA 2 cut(s) 41, 792
MluCI AATT 4 cut(s) 151, 497, 594, 602
MluNI TGGCCA 2 cut(s) 41, 792
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 7 cut(s) 186, 358, 439, 689, 870, 932, 1061
MmeI TCCRAC 3 cut(s) 402, 717, 911
Mox20I TGGCCA 2 cut(s) 41, 792
Mph1103I ATGCAT 1 cut(s) 417
MscI TGGCCA 2 cut(s) 41, 792
MseI TTAA 1 cut(s) 120
Msp20I TGGCCA 2 cut(s) 41, 792
MspA1I CMGCKG 2 cut(s) 160, 172
MspI CCGG 4 cut(s) 425, 611, 617, 633
MspR9I CCNGG 2 cut(s) 135, 618
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 7 cut(s) 34, 169, 252, 440, 620, 629, 989
NarI GGCGCC 1 cut(s) 614
NciI CCSGG 1 cut(s) 618
NlaIII CATG 4 cut(s) 337, 443, 477, 1059
NlaIV GGNNCC 4 cut(s) 615, 1062, 1128, 1137
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 2 cut(s) 292, 469
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 839
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 399
PctI GAATGC 1 cut(s) 254
PfeI GAWTC 4 cut(s) 71, 711, 755, 898
Pfl23II CGTACG 2 cut(s) 127, 510
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 3 cut(s) 162, 254, 257
PleI GAGTC 7 cut(s) 186, 358, 439, 689, 870, 931, 1060
PluTI GGCGCC 1 cut(s) 617
PpsI GAGTC 7 cut(s) 186, 358, 439, 689, 870, 931, 1060
Ppu21I YACGTR 1 cut(s) 514
Psp124BI GAGCTC 2 cut(s) 81, 625
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspLI CGTACG 2 cut(s) 127, 510
PspN4I GGNNCC 4 cut(s) 615, 1062, 1128, 1137
PspPI GGNCC 2 cut(s) 580, 630
PspXI VCTCGAGB 1 cut(s) 839
PstI CTGCAG 1 cut(s) 906
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 4 cut(s) 105, 137, 235, 747
PvuII CAGCTG 2 cut(s) 160, 172
RsaI GTAC 3 cut(s) 129, 344, 512
RsaNI GTAC 3 cut(s) 128, 343, 511
SacI GAGCTC 2 cut(s) 81, 625
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 3 cut(s) 161, 253, 256
Sau96I GGNCC 2 cut(s) 580, 630
SchI GAGTC 7 cut(s) 186, 358, 439, 689, 870, 932, 1061
ScrFI CCNGG 2 cut(s) 135, 618
SduI GDGCHC 4 cut(s) 81, 625, 840, 1138
SfaNI GCATC 2 cut(s) 424, 565
SfcI CTRYAG 3 cut(s) 217, 902, 1115
SfoI GGCGCC 1 cut(s) 615
Sfr274I CTCGAG 1 cut(s) 839
SinI GGWCC 1 cut(s) 580
SlaI CTCGAG 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 4 cut(s) 151, 497, 594, 602
SsiI CCGC 5 cut(s) 100, 228, 256, 432, 1112
SspDI GGCGCC 1 cut(s) 613
SspI AATATT 1 cut(s) 485
SstI GAGCTC 2 cut(s) 81, 625
StyD4I CCNGG 2 cut(s) 133, 616
TaaI ACNGT 5 cut(s) 127, 808, 892, 1091, 1126
TaiI ACGT 6 cut(s) 133, 299, 375, 396, 471, 516
TaqI TCGA 5 cut(s) 273, 465, 669, 840, 926
TasI AATT 4 cut(s) 151, 497, 594, 602
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 4 cut(s) 71, 711, 755, 898
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 1109
TseFI GTSAC 2 cut(s) 292, 469
TseI GCWGC 2 cut(s) 160, 252
Tsp45I GTSAC 2 cut(s) 292, 469
TspDTI ATGAA 4 cut(s) 428, 560, 614, 691
TspGWI ACGGA 2 cut(s) 305, 330
TspRI CASTG 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 2 cut(s) 497, 602
XceI RCATGY 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 926
ZraI GACGTC 1 cut(s) 469
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.