AT4G25890

60S acidic ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008579
Physical Location & Seq
Reverse (-)
13159381 .. 13160565
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25890.1

Sequence Viewer

Length: 360 bp
ATGGGAGTATTCACATTCGTATGCAAGAATGGAGGCGGCGCGTGGAGCGCGAAGCAACACGAAGGAGAACTTGAATCTTCAGCTTCTTCTACCTACGAGCTCCAGCGTAAGCTCGTTCAGGTATCTCTCTCCGCTGATTCCTCCGGTGGCGTTCAGTCTTCGTTTTCTCTTGTCTCCCCTACCTCCGCCGTATTCCAGGTAATTGTTGGTGGTGGTGGTGGTGGAGGATTCTCTGCTGGAGGAGCTGCCTCAAGTGGAGGTGGTGCGGGTGAAGCTGCAGCTGCCCCTAAGGAAGATGAGAAGAAGAAAGAAGAATCAGAAGAGGAAGAAGGAGACTTCGGATTCGATCTCTTTGGTTAA

Protein Analysis

119

Amino Acids

11.85

Weight (kDa)

4.42

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 69 - 118 1.7e-09 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 50
AciI CCGC 4 cut(s) 36, 132, 186, 266
AcuI CTGAAG 1 cut(s) 63
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 6 cut(s) 83, 100, 112, 245, 275, 281
AluI AGCT 6 cut(s) 83, 100, 112, 245, 275, 281
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 178, 327
ApeKI GCWGC 4 cut(s) 245, 275, 278, 281
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 2 cut(s) 41, 50
AsuHPI GGTGA 1 cut(s) 281
AxyI CCTNAGG 1 cut(s) 288
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 232, 262, 268, 290
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 178, 327
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 5 cut(s) 37, 246, 276, 279, 282
BlsI GCNGC 5 cut(s) 38, 247, 277, 280, 283
Bme1390I CCNGG 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 86, 258
BpuEI CTTGAG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 143
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 4 cut(s) 232, 262, 268, 290
Bsh1236I CGCG 2 cut(s) 41, 50
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 144
BsmAI GTCTC 2 cut(s) 178, 327
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 1 cut(s) 346
BspACI CCGC 4 cut(s) 36, 132, 186, 266
BspFNI CGCG 2 cut(s) 41, 50
BspMAI CTGCAG 1 cut(s) 280
BssMI GATC 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 288
BstFNI CGCG 2 cut(s) 41, 50
BstHHI GCGC 2 cut(s) 41, 50
BstKTI GATC 1 cut(s) 349
BstMAI GTCTC 2 cut(s) 178, 327
BstMBI GATC 1 cut(s) 346
BstMWI GCNNNNNNNGC 5 cut(s) 45, 47, 242, 272, 281
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 2 cut(s) 41, 50
BstV1I GCAGC 4 cut(s) 232, 262, 268, 290
BstV2I GAAGAC 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 288
CfoI GCGC 2 cut(s) 41, 50
CviJI RGCY 6 cut(s) 83, 100, 112, 245, 275, 281
CviKI_1 RGCY 6 cut(s) 83, 100, 112, 245, 275, 281
DdeI CTNAG 1 cut(s) 288
DpnI GATC 1 cut(s) 348
DpnII GATC 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
EciI GGCGGA 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 63
Eco81I CCTNAGG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 102
FaiI YATR 1 cut(s) 22
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FauI CCCGC 1 cut(s) 259
Fnu4HI GCNGC 5 cut(s) 37, 246, 276, 279, 282
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 5 cut(s) 37, 246, 276, 279, 282
GlaI GCGC 2 cut(s) 40, 49
GluI GCNGC 5 cut(s) 37, 246, 276, 279, 282
GsuI CTGGAG 2 cut(s) 86, 258
HapII CCGG 1 cut(s) 144
HhaI GCGC 2 cut(s) 41, 50
Hin6I GCGC 2 cut(s) 39, 48
HinP1I GCGC 2 cut(s) 39, 48
HinfI GANTC 5 cut(s) 74, 137, 228, 314, 342
HpaII CCGG 1 cut(s) 144
HphI GGTGA 1 cut(s) 281
Hpy188I TCNGA 2 cut(s) 319, 341
HpyAV CCTTC 2 cut(s) 56, 323
HpyCH4V TGCA 2 cut(s) 24, 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 47, 242, 272, 281
HpyF3I CTNAG 1 cut(s) 288
HspAI GCGC 2 cut(s) 39, 48
Kzo9I GATC 1 cut(s) 346
LmnI GCTCC 3 cut(s) 45, 105, 242
LpnPI CCDG 6 cut(s) 104, 116, 157, 182, 209, 222
Lsp1109I GCAGC 4 cut(s) 232, 262, 268, 290
MalI GATC 1 cut(s) 348
MboI GATC 1 cut(s) 346
MboII GAAGA 9 cut(s) 69, 78, 150, 305, 313, 316, 323, 332, 338
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 1 cut(s) 201
MnlI CCTC 8 cut(s) 26, 151, 193, 218, 233, 251, 259, 316
MseI TTAA 1 cut(s) 358
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 2 cut(s) 134, 281
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 2 cut(s) 41, 50
MwoI GCNNNNNNNGC 5 cut(s) 45, 47, 242, 272, 281
NdeII GATC 1 cut(s) 346
PfeI GAWTC 5 cut(s) 74, 137, 228, 314, 342
PkrI GCNGC 5 cut(s) 38, 247, 277, 280, 283
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PstI CTGCAG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 19
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 5 cut(s) 37, 246, 276, 279, 282
Sau3AI GATC 1 cut(s) 346
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 1 cut(s) 102
SfcI CTRYAG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 1 cut(s) 201
SsiI CCGC 4 cut(s) 36, 132, 186, 266
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 195
TaqI TCGA 1 cut(s) 345
TasI AATT 1 cut(s) 201
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 5 cut(s) 74, 137, 228, 314, 342
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TseI GCWGC 4 cut(s) 245, 275, 278, 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.