FvH4_4g07630

60S acidic ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008579
Physical Location & Seq
Reverse (-)
7042572 .. 7043394
823 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07630.t1

Sequence Viewer

Length: 363 bp
ATGGGAGTTTTCACCTTCGTCGTCAAGAACTCAGGAGGAGAATGGAAAGCGAAGCAGCACGAGGGCGAACTCGAGGCCTGTGCTCCTTCCTCCTACGAGCTCCAGAGGAAGCTCGTCCAGGCCGCTCTCTCTTCTGACAGCTCCGGCGGCGTTCAGAGCTCATACTCTCCCGTCACTCCTTCCTCTGCTGTCTTCCAGGTGATCATTGGTGGTGGTGGCGGTGGTGGTGCCTTCATTGGTGGAGGTGCTGCCACAGCTCCAGCAGGTGGTGCAGCAGCTACTGCAGAGGCTCCCCCAGCTGAGGAGAAGAAGGAAGAGAAGGAGGAGAGTGATGATGACATGGGATTCTCTCTCTTTGATTAA

Protein Analysis

121

Amino Acids

12.05

Weight (kDa)

4.44

Isoelectric Point (pI)

65.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 71 - 119 1.8e-11 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 266
AccBSI CCGCTC 1 cut(s) 125
AciI CCGC 3 cut(s) 123, 147, 219
AfiI CCNNNNNNNGG 2 cut(s) 266, 301
AjnI CCWGG 2 cut(s) 117, 195
AluBI AGCT 7 cut(s) 100, 112, 141, 159, 257, 278, 299
AluI AGCT 7 cut(s) 100, 112, 141, 159, 257, 278, 299
Alw21I GWGCWC 3 cut(s) 85, 102, 161
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 2 cut(s) 75, 120
ApeKI GCWGC 4 cut(s) 55, 248, 272, 275
AsuHPI GGTGA 2 cut(s) 4, 211
AvaI CYCGRG 1 cut(s) 71
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 2 cut(s) 102, 161
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 3 cut(s) 85, 102, 161
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 4 cut(s) 67, 235, 284, 287
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 2 cut(s) 119, 197
BclI TGATCA 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 282
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 6 cut(s) 56, 123, 148, 249, 273, 276
BlsI GCNGC 6 cut(s) 57, 124, 149, 250, 274, 277
Bme1390I CCNGG 2 cut(s) 119, 197
BmeT110I CYCGRG 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 229, 291
BmrFI CCNGG 2 cut(s) 119, 197
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 2 cut(s) 86, 243
Bpu10I CCTNAGC 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 301
BseBI CCWGG 2 cut(s) 119, 197
BseLI CCNNNNNNNGG 2 cut(s) 266, 301
BseMII CTCAG 2 cut(s) 45, 291
BseRI GAGGAG 3 cut(s) 51, 317, 338
BseXI GCAGC 4 cut(s) 67, 235, 284, 287
BseYI CCCAGC 1 cut(s) 295
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 2 cut(s) 77, 122
BshNI GGYRCC 1 cut(s) 227
BsiHKAI GWGCWC 3 cut(s) 85, 102, 161
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 266, 301
BsnI GGCC 2 cut(s) 77, 122
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 85, 102, 161
Bsp143I GATC 1 cut(s) 201
BspACI CCGC 3 cut(s) 123, 147, 219
BspANI GGCC 2 cut(s) 77, 122
BspCNI CTCAG 2 cut(s) 44, 292
BspLI GGNNCC 2 cut(s) 229, 291
BspMAI CTGCAG 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 125
BssMI GATC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 119, 197
Bst6I CTCTTC 2 cut(s) 136, 309
BstAPI GCANNNNNTGC 2 cut(s) 269, 281
BstDEI CTNAG 2 cut(s) 31, 300
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 6 cut(s) 147, 156, 254, 269, 281, 296
BstNI CCWGG 2 cut(s) 119, 197
BstSCI CCNGG 2 cut(s) 117, 195
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 4 cut(s) 67, 235, 284, 287
BstV2I GAAGAC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 77, 122
BveI ACCTGC 1 cut(s) 254
CaiI CAGNNNCTG 1 cut(s) 281
CviAII CATG 1 cut(s) 340
DdeI CTNAG 2 cut(s) 31, 300
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
Eam1104I CTCTTC 2 cut(s) 136, 309
EarI CTCTTC 2 cut(s) 136, 309
Ecl136II GAGCTC 2 cut(s) 100, 159
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 2 cut(s) 102, 161
Eco53kI GAGCTC 2 cut(s) 100, 159
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 2 cut(s) 100, 159
EcoRII CCWGG 2 cut(s) 117, 195
EcoT38I GRGCYC 2 cut(s) 102, 161
FaeI CATG 1 cut(s) 343
FaiI YATR 2 cut(s) 163, 341
FatI CATG 1 cut(s) 339
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 6 cut(s) 56, 123, 148, 249, 273, 276
FriOI GRGCYC 2 cut(s) 102, 161
Fsp4HI GCNGC 6 cut(s) 56, 123, 148, 249, 273, 276
GluI GCNGC 6 cut(s) 56, 123, 148, 249, 273, 276
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 86, 243
HaeIII GGCC 2 cut(s) 77, 122
HapII CCGG 1 cut(s) 144
Hin1II CATG 1 cut(s) 343
HinfI GANTC 1 cut(s) 345
HpaII CCGG 1 cut(s) 144
HphI GGTGA 2 cut(s) 4, 211
Hpy188I TCNGA 2 cut(s) 136, 156
Hpy188III TCNNGA 3 cut(s) 25, 33, 103
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 6 cut(s) 25, 96, 189, 241, 304, 313
HpyCH4V TGCA 2 cut(s) 272, 284
HpyF10VI GCNNNNNNNGC 6 cut(s) 147, 156, 254, 269, 281, 296
HpyF3I CTNAG 2 cut(s) 31, 300
Hsp92II CATG 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 5 cut(s) 88, 105, 146, 262, 295
Lsp1109I GCAGC 4 cut(s) 67, 235, 284, 287
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 203
MbiI CCGCTC 1 cut(s) 125
MboI GATC 1 cut(s) 201
MboII GAAGA 4 cut(s) 123, 184, 319, 326
MhlI GDGCHC 3 cut(s) 85, 102, 161
MseI TTAA 1 cut(s) 361
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 119, 197
MvaI CCWGG 2 cut(s) 119, 197
MwoI GCNNNNNNNGC 6 cut(s) 147, 156, 254, 269, 281, 296
NdeII GATC 1 cut(s) 201
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 229, 291
NmuCI GTSAC 1 cut(s) 172
PaeR7I CTCGAG 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 254
PceI AGGCCT 1 cut(s) 77
PfeI GAWTC 1 cut(s) 345
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 6 cut(s) 57, 124, 149, 250, 274, 277
Psp124BI GAGCTC 2 cut(s) 102, 161
Psp6I CCWGG 2 cut(s) 117, 195
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 2 cut(s) 117, 195
PspN4I GGNNCC 2 cut(s) 229, 291
PspXI VCTCGAGB 1 cut(s) 71
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 299
SacI GAGCTC 2 cut(s) 102, 161
SaqAI TTAA 1 cut(s) 361
SatI GCNGC 6 cut(s) 56, 123, 148, 249, 273, 276
Sau3AI GATC 1 cut(s) 201
ScrFI CCNGG 2 cut(s) 119, 197
SduI GDGCHC 3 cut(s) 85, 102, 161
SfcI CTRYAG 1 cut(s) 282
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 3 cut(s) 123, 147, 219
SstI GAGCTC 2 cut(s) 102, 161
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 117, 195
TaqI TCGA 1 cut(s) 72
TauI GCSGC 2 cut(s) 125, 150
TfiI GAWTC 1 cut(s) 345
Tru1I TTAA 1 cut(s) 361
Tru9I TTAA 1 cut(s) 361
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 4 cut(s) 55, 248, 272, 275
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.