AT4G27120

DDRGK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008682
Physical Location & Seq
Reverse (-)
13601948 .. 13604494
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27120.2

Sequence Viewer

Length: 897 bp
ATGGAGGAGATATTTGCTCTGATAGTCTCAATGATTCTCATAGTTGCGGTGATTCCTTTGTTCTTTTGGAAACGTCGGAGAGATGCTAGATCACGGGAGGAAGTTGCTGAACCACCACAGGTTCAACCTCGTGAAAACGTGGCACGTGCTGGTGGTGGACGGAGGATGCGTAGGAGACCTGCAGCTTCTGGTGCTAGCTCCTCTACATCCAATGTTCAAGAAAACGGCAGTGGGAGTGAAGATGAAGATGAAGATGAAGCTGGTGGAACTCAAGCCAGAGCATCAAAGAAGAAAGAGAAGAAGCGACAAGAGCGGGAAGCACAAAGACAAGCTGAGGAAGCTACACGCGAGTCAAGGAATACAAAACAAGATTGGTATGCAGAAATGCGGAGGAAGAAGGATGAAGAACGCGAGGCAGAAGAGCTGAAGTTGGAAGAAGAAGAAAAAGCGCGACAAGCCAAAGAAGAAGAGGCAGCTGCGTTAGAGTTTGACAAATGGAAAGGGGAATTTTCAGTTGATGCTGAAGGTACTACAGAGGAAGTGCAAGGTGGGAATCAAGATTTGCTTTCAGAATTTGTTGAATACATAAAGAAACAGAAATGTGTTCCACTTGAAGATCTCGCTGCAGAATTTCATCTACGTACTCAGGAATGCATCAACAGGATCGCCTCTTTGGAGAGCATTGGGCGGCTCTCGGGGGTCATGGATGATAGAGGGAAATACATTTACATATCAATGGAAGAGATGAATGCGGTTGCAGATTACATCAAACGTCAAGGAAGAGTTAGTATCTCACATCTAGCAAGCAAGTCAAACCAGTTCATCGACTTGGAGCCCAAAGTACAGCATCAGCTGACTGAAGAAATTAGCGGCATGGAAGAGATCTCAGTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDRGK

Protein Analysis

298

Amino Acids

34.02

Weight (kDa)

5.08

Isoelectric Point (pI)

62.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDRGK PF09756 94 - 276 6.2e-53 DDRGK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 3 cut(s) 348, 411, 451
AciI CCGC 6 cut(s) 47, 313, 388, 688, 752, 870
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 3 cut(s) 506, 572, 629
AcuI CTGAAG 3 cut(s) 446, 543, 879
AcvI CACGTG 1 cut(s) 146
AfaI GTAC 3 cut(s) 529, 643, 843
AgsI TTSAA 4 cut(s) 125, 218, 581, 614
AluBI AGCT 8 cut(s) 185, 198, 260, 332, 341, 424, 476, 853
AluI AGCT 8 cut(s) 185, 198, 260, 332, 341, 424, 476, 853
Alw26I GTCTC 2 cut(s) 31, 169
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 694
ApeKI GCWGC 4 cut(s) 182, 473, 476, 623
ApoI RAATTY 3 cut(s) 506, 572, 629
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 61
AsuNHI GCTAGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 694
BanII GRGCYC 1 cut(s) 837
BauI CACGAG 1 cut(s) 129
BbrPI CACGTG 1 cut(s) 146
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 4 cut(s) 194, 463, 485, 610
BceAI ACGGC 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 31, 169
BfaI CTAG 3 cut(s) 87, 195, 800
BfmI CTRYAG 3 cut(s) 180, 531, 624
BfuAI ACCTGC 1 cut(s) 187
BglII AGATCT 2 cut(s) 616, 882
BisI GCNGC 6 cut(s) 183, 474, 477, 624, 689, 871
BlsI GCNGC 6 cut(s) 184, 475, 478, 625, 690, 872
BmeT110I CYCGRG 1 cut(s) 694
BmiI GGNNCC 1 cut(s) 834
BmsI GCATC 6 cut(s) 73, 156, 290, 508, 663, 856
BmtI GCTAGC 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 255
BsaAI YACGTR 2 cut(s) 146, 641
BsaI GGTCTC 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bse1I ACTGG 1 cut(s) 817
BseGI GGATG 4 cut(s) 171, 206, 406, 712
BseMII CTCAG 2 cut(s) 324, 659
BseNI ACTGG 1 cut(s) 817
BseRI GAGGAG 2 cut(s) 20, 190
BseXI GCAGC 4 cut(s) 194, 463, 485, 610
Bsh1236I CGCG 3 cut(s) 348, 411, 451
BsiHKCI CYCGRG 1 cut(s) 694
BsmAI GTCTC 2 cut(s) 31, 169
BsmI GAATGC 2 cut(s) 656, 754
Bso31I GGTCTC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 694
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 4 cut(s) 89, 616, 663, 882
BspACI CCGC 6 cut(s) 47, 313, 388, 688, 752, 870
BspCNI CTCAG 2 cut(s) 325, 658
BspFNI CGCG 3 cut(s) 348, 411, 451
BspLI GGNNCC 1 cut(s) 834
BspMAI CTGCAG 2 cut(s) 184, 628
BspMI ACCTGC 1 cut(s) 187
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 671
BspQI GCTCTTC 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 313
BsrI ACTGG 1 cut(s) 817
BssMI GATC 4 cut(s) 89, 616, 663, 882
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
Bst6I CTCTTC 5 cut(s) 414, 462, 735, 775, 873
BstBAI YACGTR 2 cut(s) 146, 641
BstC8I GCNNGC 2 cut(s) 196, 805
BstDEI CTNAG 4 cut(s) 333, 645, 886, 894
BstF5I GGATG 4 cut(s) 171, 206, 406, 712
BstFNI CGCG 3 cut(s) 348, 411, 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 4 cut(s) 92, 619, 666, 885
BstMAI GTCTC 2 cut(s) 31, 169
BstMBI GATC 4 cut(s) 89, 616, 663, 882
BstMWI GCNNNNNNNGC 4 cut(s) 191, 310, 338, 455
BstSFI CTRYAG 3 cut(s) 180, 531, 624
BstSNI TACGTA 1 cut(s) 641
BstUI CGCG 3 cut(s) 348, 411, 451
BstV1I GCAGC 4 cut(s) 194, 463, 485, 610
BstX2I RGATCY 2 cut(s) 616, 882
BstYI RGATCY 2 cut(s) 616, 882
BtsCI GGATG 4 cut(s) 171, 206, 406, 712
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 196, 805
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 1 cut(s) 451
Csp6I GTAC 3 cut(s) 528, 642, 842
CviAII CATG 2 cut(s) 703, 874
CviQI GTAC 3 cut(s) 528, 642, 842
DdeI CTNAG 4 cut(s) 333, 645, 886, 894
DpnI GATC 4 cut(s) 91, 618, 665, 884
DpnII GATC 4 cut(s) 89, 616, 663, 882
Eam1104I CTCTTC 5 cut(s) 414, 462, 735, 775, 873
EarI CTCTTC 5 cut(s) 414, 462, 735, 775, 873
Eco105I TACGTA 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 837
Eco31I GGTCTC 1 cut(s) 169
Eco57I CTGAAG 3 cut(s) 446, 543, 879
Eco72I CACGTG 1 cut(s) 146
Eco88I CYCGRG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 656
EcoT38I GRGCYC 1 cut(s) 837
FaeI CATG 2 cut(s) 706, 877
FaiI YATR 6 cut(s) 41, 378, 587, 704, 731, 875
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FatI CATG 2 cut(s) 702, 873
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 6 cut(s) 183, 474, 477, 624, 689, 871
FokI GGATG 4 cut(s) 178, 193, 413, 719
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 6 cut(s) 183, 474, 477, 624, 689, 871
FspBI CTAG 3 cut(s) 87, 195, 800
GlaI GCGC 1 cut(s) 450
GluI GCNGC 6 cut(s) 183, 474, 477, 624, 689, 871
HhaI GCGC 1 cut(s) 451
Hin1II CATG 2 cut(s) 706, 877
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HinfI GANTC 4 cut(s) 34, 52, 350, 553
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 21, 78, 571
Hpy188III TCNNGA 4 cut(s) 131, 218, 557, 647
Hpy8I GTNNAC 1 cut(s) 158
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 2 cut(s) 391, 518
HpyCH4IV ACGT 5 cut(s) 73, 138, 145, 640, 772
HpyCH4V TGCA 6 cut(s) 182, 380, 544, 626, 654, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 310, 338, 455
HpyF3I CTNAG 4 cut(s) 333, 645, 886, 894
HpySE526I ACGT 5 cut(s) 73, 138, 145, 640, 772
Hsp92II CATG 2 cut(s) 706, 877
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 4 cut(s) 89, 616, 663, 882
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 203, 832
LpnPI CCDG 9 cut(s) 104, 135, 174, 192, 246, 289, 632, 646, 830
Lsp1109I GCAGC 4 cut(s) 194, 463, 485, 610
LweI GCATC 6 cut(s) 73, 156, 290, 508, 663, 856
MaeI CTAG 3 cut(s) 87, 195, 800
MaeII ACGT 5 cut(s) 73, 138, 145, 640, 772
MalI GATC 4 cut(s) 91, 618, 665, 884
MbiI CCGCTC 1 cut(s) 313
MboI GATC 4 cut(s) 89, 616, 663, 882
MflI RGATCY 2 cut(s) 616, 882
MhlI GDGCHC 1 cut(s) 837
MluCI AATT 4 cut(s) 506, 572, 629, 864
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 56, 411
Mph1103I ATGCAT 1 cut(s) 656
MslI CAYNNNNRTG 1 cut(s) 734
MspA1I CMGCKG 2 cut(s) 476, 853
Mva1269I GAATGC 2 cut(s) 656, 754
MvnI CGCG 3 cut(s) 348, 411, 451
MwoI GCNNNNNNNGC 4 cut(s) 191, 310, 338, 455
NdeII GATC 4 cut(s) 89, 616, 663, 882
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 2 cut(s) 706, 877
NlaIV GGNNCC 1 cut(s) 834
NsiI ATGCAT 1 cut(s) 656
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PctI GAATGC 2 cut(s) 656, 754
PfeI GAWTC 3 cut(s) 34, 52, 553
PkrI GCNGC 6 cut(s) 184, 475, 478, 625, 690, 872
PleI GAGTC 1 cut(s) 358
PmaCI CACGTG 1 cut(s) 146
PmlI CACGTG 1 cut(s) 146
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 2 cut(s) 146, 641
PspCI CACGTG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 834
PstI CTGCAG 2 cut(s) 184, 628
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 616, 882
PvuII CAGCTG 2 cut(s) 476, 853
RsaI GTAC 3 cut(s) 529, 643, 843
RsaNI GTAC 3 cut(s) 528, 642, 842
RseI CAYNNNNRTG 1 cut(s) 734
SapI GCTCTTC 1 cut(s) 414
SatI GCNGC 6 cut(s) 183, 474, 477, 624, 689, 871
Sau3AI GATC 4 cut(s) 89, 616, 663, 882
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 1 cut(s) 837
SfaNI GCATC 6 cut(s) 73, 156, 290, 508, 663, 856
SfcI CTRYAG 3 cut(s) 180, 531, 624
SmiMI CAYNNNNRTG 1 cut(s) 734
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
SnaBI TACGTA 1 cut(s) 641
Sse9I AATT 4 cut(s) 506, 572, 629, 864
SsiI CCGC 6 cut(s) 47, 313, 388, 688, 752, 870
SspMI CTAG 3 cut(s) 87, 195, 800
TaiI ACGT 5 cut(s) 76, 141, 148, 643, 775
TaqI TCGA 1 cut(s) 825
TasI AATT 4 cut(s) 506, 572, 629, 864
TatI WGTACW 1 cut(s) 841
TauI GCSGC 2 cut(s) 691, 873
TfiI GAWTC 3 cut(s) 34, 52, 553
TscAI CASTG 1 cut(s) 235
TseI GCWGC 4 cut(s) 182, 473, 476, 623
TspDTI ATGAA 7 cut(s) 258, 264, 270, 417, 623, 761, 811
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 235
XapI RAATTY 3 cut(s) 506, 572, 629
XspI CTAG 3 cut(s) 87, 195, 800
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.