MD11G1224700.v1.1

DDRGK domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008682
Physical Location & Seq
Reverse (-)
32901111 .. 32904879
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1224700.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGAGGAAATGTTAGCAATTTTTCTTTCCATGCTTCTTGTTTTGGGATTAATTCCACTATATCTATGGAAACGCCGCCAAGATTCTCGACCGCCGCATGAACACGAAGAAGAACCTCAGGTTGCTCAGAGGGAGGCGGTAGTGCGTGCGCCTGGCGGTCGCCGGATGCGTAGGAGGCCGGCTTCTGGAGCAAGCACATCATCAGCTGCTGCACCAGCTGTAGAAGAAACCGTTGATGGAAGTGATGAGGAAGATGTTGAGGGCGAGTATGAAGACAAAGCATCCAAGAAGAGGGAAAAGAAGCGTCAAGAGCGGGAAGCACAACGACAAGCTGAACAAGCTGCACGTGAATCAAGGGTAACAAAACAAGACCGCTATGCTGAAATGCGGAGGAGGAAAGATGAGGAGCGTGAGGCACAGGAGCGTCAGCTGGAGGAAGAAGCCAAAGCTCAAAGGGCTAGGGAGGAGGAGGCTGCTGCTTTAGAGTTTGAAAAGTGGAAAGGCGAGTTTTCAATAGACGCTGAAGGTACTACAGAGAATGAAGTGCAGGATGGACGTCAGGATTTGCTGTCTGATTTTGTGGAATACATAAAGAAACATAAATGCATTCCCTTGGAAGATCTTGCTGCTGAATTTAAGTTAAGGACTCAGGAATGCATCAATCGAATTACCAATCTGGAGAGTATGGGGCGACTTTCTGGCGTCATGGACGACAGAGGGAAATACATATACATCTCCCAAGAAGAGATGCAAGCTGTGGCAGACTACATTAAGCGTCAAGGAAGGGTAAGCATTTCGCATCTCGCTAGCAAGTCCAACCAGTTCATAGACTTGGAGCCCAAAGCGCAGTTTGTTGAAGAAACCGGTACTGGGGCAGAGATAACTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDRGK

Protein Analysis

298

Amino Acids

34.14

Weight (kDa)

5.12

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDRGK PF09756 94 - 277 9.3e-53 DDRGK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 559
AccBSI CCGCTC 1 cut(s) 313
AciI CCGC 8 cut(s) 76, 92, 95, 137, 156, 313, 373, 388
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 2 cut(s) 556, 702
AfaI GTAC 2 cut(s) 529, 868
AfiI CCNNNNNNNGG 3 cut(s) 185, 291, 870
AgeI ACCGGT 1 cut(s) 863
AgsI TTSAA 3 cut(s) 491, 513, 857
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 7 cut(s) 206, 218, 332, 341, 430, 449, 755
AluI AGCT 7 cut(s) 206, 218, 332, 341, 430, 449, 755
AlwNI CAGNNNCTG 1 cut(s) 209
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 6 cut(s) 206, 209, 341, 473, 476, 626
ApoI RAATTY 1 cut(s) 632
AseI ATTAAT 1 cut(s) 50
AsiGI ACCGGT 1 cut(s) 863
AspLEI GCGC 2 cut(s) 151, 847
AsuNHI GCTAGC 1 cut(s) 806
AxyI CCTNAGG 1 cut(s) 117
BanII GRGCYC 1 cut(s) 840
BbrPI CACGTG 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 279
BbvI GCAGC 6 cut(s) 193, 196, 328, 460, 463, 613
BccI CCATC 2 cut(s) 230, 545
BciT130I CCWGG 1 cut(s) 153
BfaI CTAG 2 cut(s) 459, 807
BfmI CTRYAG 2 cut(s) 219, 531
BglII AGATCT 1 cut(s) 619
BisI GCNGC 8 cut(s) 76, 95, 207, 210, 342, 474, 477, 627
BlsI GCNGC 8 cut(s) 77, 96, 208, 211, 343, 475, 478, 628
Bme1390I CCNGG 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 837
BmrFI CCNGG 1 cut(s) 153
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 5 cut(s) 156, 290, 666, 738, 808
BmtI GCTAGC 1 cut(s) 810
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 3 cut(s) 207, 452, 698
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 2 cut(s) 556, 702
BsaJI CCNNGG 1 cut(s) 612
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 291, 870
Bse118I RCCGGY 2 cut(s) 178, 863
Bse1I ACTGG 2 cut(s) 820, 874
Bse21I CCTNAGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 3 cut(s) 171, 281, 556
BseLI CCNNNNNNNGG 3 cut(s) 185, 291, 870
BseMII CTCAG 3 cut(s) 131, 140, 662
BseNI ACTGG 2 cut(s) 820, 874
BseRI GAGGAG 4 cut(s) 406, 419, 479, 482
BseXI GCAGC 6 cut(s) 193, 196, 328, 460, 463, 613
BsgI GTGCAG 3 cut(s) 195, 327, 566
Bsh1285I CGRYCG 2 cut(s) 92, 160
BshFI GGCC 1 cut(s) 178
BshTI ACCGGT 1 cut(s) 863
BsiEI CGRYCG 2 cut(s) 92, 160
BsiSI CCGG 3 cut(s) 163, 179, 864
BslI CCNNNNNNNGG 3 cut(s) 185, 291, 870
BsmI GAATGC 2 cut(s) 606, 659
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 1 cut(s) 619
BspACI CCGC 8 cut(s) 76, 92, 95, 137, 156, 313, 373, 388
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 3 cut(s) 130, 139, 661
BspLI GGNNCC 1 cut(s) 837
BspOI GCTAGC 1 cut(s) 810
BsrBI CCGCTC 1 cut(s) 313
BsrFI RCCGGY 2 cut(s) 178, 863
BsrI ACTGG 2 cut(s) 820, 874
BssAI RCCGGY 2 cut(s) 178, 863
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 1 cut(s) 619
BssNI GRCGYC 2 cut(s) 556, 702
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 232, 886
Bst6I CTCTTC 2 cut(s) 284, 738
BstACI GRCGYC 2 cut(s) 556, 702
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 5 cut(s) 147, 180, 193, 753, 808
BstDEI CTNAG 3 cut(s) 117, 126, 648
BstF5I GGATG 3 cut(s) 171, 281, 556
BstHHI GCGC 2 cut(s) 151, 847
BstKTI GATC 1 cut(s) 622
BstMBI GATC 1 cut(s) 619
BstMCI CGRYCG 2 cut(s) 92, 160
BstMWI GCNNNNNNNGC 7 cut(s) 175, 188, 215, 310, 338, 455, 844
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 2 cut(s) 219, 531
BstV1I GCAGC 6 cut(s) 193, 196, 328, 460, 463, 613
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 3 cut(s) 171, 281, 556
Cac8I GCNNGC 5 cut(s) 147, 180, 193, 753, 808
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 2 cut(s) 151, 847
Cfr10I RCCGGY 2 cut(s) 178, 863
CseI GACGC 5 cut(s) 293, 413, 527, 691, 764
Csp6I GTAC 2 cut(s) 528, 867
CspAI ACCGGT 1 cut(s) 863
CviAII CATG 3 cut(s) 31, 98, 706
CviQI GTAC 2 cut(s) 528, 867
DdeI CTNAG 3 cut(s) 117, 126, 648
DpnI GATC 1 cut(s) 621
DpnII GATC 1 cut(s) 619
Eam1104I CTCTTC 2 cut(s) 284, 738
EarI CTCTTC 2 cut(s) 284, 738
Eco130I CCWWGG 1 cut(s) 612
Eco24I GRGCYC 1 cut(s) 840
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 347
Eco81I CCTNAGG 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 612
EcoT22I ATGCAT 2 cut(s) 608, 659
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 3 cut(s) 34, 101, 709
FatI CATG 3 cut(s) 30, 97, 705
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 8 cut(s) 76, 95, 207, 210, 342, 474, 477, 627
FokI GGATG 3 cut(s) 178, 268, 563
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 8 cut(s) 76, 95, 207, 210, 342, 474, 477, 627
FspBI CTAG 2 cut(s) 459, 807
GlaI GCGC 2 cut(s) 150, 846
GluI GCNGC 8 cut(s) 76, 95, 207, 210, 342, 474, 477, 627
GsuI CTGGAG 3 cut(s) 207, 452, 698
HaeIII GGCC 1 cut(s) 178
HapII CCGG 3 cut(s) 163, 179, 864
HgaI GACGC 5 cut(s) 293, 413, 527, 691, 764
HhaI GCGC 2 cut(s) 151, 847
Hin1I GRCGYC 2 cut(s) 556, 702
Hin1II CATG 3 cut(s) 34, 101, 709
Hin6I GCGC 2 cut(s) 149, 845
HinP1I GCGC 2 cut(s) 149, 845
HinfI GANTC 3 cut(s) 83, 350, 646
HpaII CCGG 3 cut(s) 163, 179, 864
Hpy188I TCNGA 2 cut(s) 129, 574
Hpy188III TCNNGA 6 cut(s) 87, 186, 308, 560, 650, 677
HpyAV CCTTC 2 cut(s) 518, 777
HpyCH4III ACNGT 2 cut(s) 232, 886
HpyCH4IV ACGT 2 cut(s) 346, 556
HpyCH4V TGCA 6 cut(s) 212, 344, 547, 606, 657, 751
HpyF10VI GCNNNNNNNGC 7 cut(s) 175, 188, 215, 310, 338, 455, 844
HpyF3I CTNAG 3 cut(s) 117, 126, 648
HpySE526I ACGT 2 cut(s) 346, 556
Hsp92I GRCGYC 2 cut(s) 556, 702
Hsp92II CATG 3 cut(s) 34, 101, 709
HspAI GCGC 2 cut(s) 149, 845
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
Kzo9I GATC 1 cut(s) 619
LmnI GCTCC 4 cut(s) 188, 406, 421, 835
Lsp1109I GCAGC 6 cut(s) 193, 196, 328, 460, 463, 613
LweI GCATC 5 cut(s) 156, 290, 666, 738, 808
MaeI CTAG 2 cut(s) 459, 807
MaeII ACGT 2 cut(s) 346, 556
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 621
MbiI CCGCTC 1 cut(s) 313
MboI GATC 1 cut(s) 619
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 840
MluCI AATT 4 cut(s) 18, 51, 632, 666
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 840
Mph1103I ATGCAT 2 cut(s) 608, 659
MroNI GCCGGC 1 cut(s) 178
MseI TTAA 4 cut(s) 50, 636, 641, 771
MspA1I CMGCKG 3 cut(s) 206, 218, 430
MspI CCGG 3 cut(s) 163, 179, 864
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 2 cut(s) 606, 659
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 7 cut(s) 175, 188, 215, 310, 338, 455, 844
NaeI GCCGGC 1 cut(s) 180
NdeII GATC 1 cut(s) 619
NgoMIV GCCGGC 1 cut(s) 178
NheI GCTAGC 1 cut(s) 806
NlaIII CATG 3 cut(s) 34, 101, 709
NlaIV GGNNCC 1 cut(s) 837
NsiI ATGCAT 2 cut(s) 608, 659
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PctI GAATGC 2 cut(s) 606, 659
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 83, 350
PinAI ACCGGT 1 cut(s) 863
PkrI GCNGC 8 cut(s) 77, 96, 208, 211, 343, 475, 478, 628
PleI GAGTC 1 cut(s) 640
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 1 cut(s) 640
Ppu21I YACGTR 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 151
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 837
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 619
PvuII CAGCTG 3 cut(s) 206, 218, 430
RsaI GTAC 2 cut(s) 529, 868
RsaNI GTAC 2 cut(s) 528, 867
SaqAI TTAA 4 cut(s) 50, 636, 641, 771
SatI GCNGC 8 cut(s) 76, 95, 207, 210, 342, 474, 477, 627
Sau3AI GATC 1 cut(s) 619
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 5 cut(s) 156, 290, 666, 738, 808
SfcI CTRYAG 2 cut(s) 219, 531
Sse9I AATT 4 cut(s) 18, 51, 632, 666
SsiI CCGC 8 cut(s) 76, 92, 95, 137, 156, 313, 373, 388
SspMI CTAG 2 cut(s) 459, 807
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 2 cut(s) 232, 886
TaiI ACGT 2 cut(s) 349, 559
TaqI TCGA 2 cut(s) 88, 664
TasI AATT 4 cut(s) 18, 51, 632, 666
TauI GCSGC 2 cut(s) 78, 97
TfiI GAWTC 2 cut(s) 83, 350
Tru1I TTAA 4 cut(s) 50, 636, 641, 771
Tru9I TTAA 4 cut(s) 50, 636, 641, 771
TseI GCWGC 6 cut(s) 206, 209, 341, 473, 476, 626
TspDTI ATGAA 4 cut(s) 114, 285, 555, 814
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 2 cut(s) 459, 807
ZraI GACGTC 1 cut(s) 557
Zsp2I ATGCAT 2 cut(s) 608, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.