MD03G1210000.v1.1

DDRGK domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008682
Physical Location & Seq
Reverse (-)
28919233 .. 28922324
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1210000.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGAGGAAATGTTGGCAATTTTTCTTTCTATGCTTCTTGTTTTGGGATTAATTCCACTATTTCTATGGAAACGCCGTCAAGATTCTCGATCTTCGCATGAACACGAAGAAGAACCTCAGGTTGCTCAGAGGGAGGCGGTGGTGCGTGCGCCTGGCGGTCGCTGGATCCGTAGGAGGCCGGCTTCTGGAGCAAGCACATCAGCTGCTGCTGCACCAGGTGTAGAAGAATCCGTTGAGGGAAGTGATGGGGAAGATGTTGAGGGCGAGTATGATGCTAAAGCATCCAAGAAGAGGGAAAAGAAGCGCCAAGAGCGGGAAGCACAACGACAAGCTGAACAAGCTGCACGTGAATCAAGGGTAACAAAACAAGACCGCTATGCTGAAATGCGGAGGAGGAAAGATGAGGAGCGTGAAGCACAGGAGCGTCAGCTGGAGGAAGAAGCCAAAGCTCAAAAGGCTAGGGAGGAGGAAGCTGCTGCTTTAGAGTTTGAGAAGTGGAAAGGCGAGTTTTCAATAGATGCTGAAGGTATGACCGAGAATGAAGTGCAGGATGGATGTCAGGATTTGCTTTCTGATTTTGTGGAATACATAAAGAAACATAAATGCATTCCTCTGGAAGATCTTGCTGCTGAATCTAAGTTAAGGACTCAGGAATGCATCTATCGAATTACCAATCTCGAGAGTATGGGGCGACTTTCTGGCGTCATGGATGACAGAGGGAAATACATATACATCTCCCAAGAGGAGATGCAAGCTGTAGCAGACTACATTAAGCGTCAGGGAAGGGTAAGCATTTCGCATCTTGCTAGCAAGTCCAACCAGTTCATAGACTTGGAGCCAAAAGCGCAGTTCGTTGAAGAAATCAGCGGCGGGACGGAGATAACTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDRGK

Protein Analysis

298

Amino Acids

33.92

Weight (kDa)

5.11

Isoelectric Point (pI)

61.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDRGK PF09756 93 - 277 2.8e-50 DDRGK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 313
AciI CCGC 7 cut(s) 137, 156, 313, 373, 388, 867, 870
AclWI GGATC 2 cut(s) 160, 173
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 702
AdeI CACNNNGTG 1 cut(s) 218
AfiI CCNNNNNNNGG 3 cut(s) 185, 291, 313
AgsI TTSAA 2 cut(s) 513, 857
AjnI CCWGG 2 cut(s) 151, 214
AluBI AGCT 7 cut(s) 203, 332, 341, 430, 449, 473, 755
AluI AGCT 7 cut(s) 203, 332, 341, 430, 449, 473, 755
AlwI GGATC 2 cut(s) 160, 173
AlwNI CAGNNNCTG 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 677
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 7 cut(s) 203, 206, 209, 341, 473, 476, 626
AseI ATTAAT 1 cut(s) 50
AspLEI GCGC 3 cut(s) 151, 306, 847
AsuNHI GCTAGC 1 cut(s) 806
AvaI CYCGRG 1 cut(s) 677
AxyI CCTNAGG 1 cut(s) 117
BamHI GGATCC 1 cut(s) 165
BbrPI CACGTG 1 cut(s) 347
BbvI GCAGC 7 cut(s) 190, 193, 196, 328, 460, 463, 613
BccI CCATC 2 cut(s) 239, 545
BceAI ACGGC 1 cut(s) 60
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 2 cut(s) 153, 216
BfaI CTAG 2 cut(s) 459, 807
BfmI CTRYAG 1 cut(s) 756
BfoI RGCGCY 1 cut(s) 307
BglII AGATCT 1 cut(s) 619
BisI GCNGC 8 cut(s) 204, 207, 210, 342, 474, 477, 627, 868
BlsI GCNGC 8 cut(s) 205, 208, 211, 343, 475, 478, 628, 869
Bme1390I CCNGG 2 cut(s) 153, 216
BmeT110I CYCGRG 1 cut(s) 677
BmiI GGNNCC 2 cut(s) 167, 837
BmrFI CCNGG 2 cut(s) 153, 216
BmsI GCATC 6 cut(s) 262, 290, 508, 666, 738, 808
BmtI GCTAGC 1 cut(s) 810
BpmI CTGGAG 2 cut(s) 207, 452
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 702
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 291, 313
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 820
Bse21I CCTNAGG 1 cut(s) 117
BseBI CCWGG 2 cut(s) 153, 216
BseGI GGATG 4 cut(s) 281, 556, 560, 715
BseLI CCNNNNNNNGG 3 cut(s) 185, 291, 313
BseMII CTCAG 3 cut(s) 131, 140, 662
BseNI ACTGG 1 cut(s) 820
BseRI GAGGAG 4 cut(s) 406, 419, 479, 758
BseXI GCAGC 7 cut(s) 190, 193, 196, 328, 460, 463, 613
BsgI GTGCAG 3 cut(s) 195, 327, 566
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 3 cut(s) 185, 291, 313
BsmFI GGGAC 1 cut(s) 886
BsmI GAATGC 2 cut(s) 606, 659
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 677
Bsp143I GATC 3 cut(s) 89, 165, 619
BspACI CCGC 7 cut(s) 137, 156, 313, 373, 388, 867, 870
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 3 cut(s) 130, 139, 661
BspLI GGNNCC 2 cut(s) 167, 837
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 160, 173
BsrBI CCGCTC 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 1 cut(s) 820
BssAI RCCGGY 1 cut(s) 178
BssMI GATC 3 cut(s) 89, 165, 619
BssNI GRCGYC 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 153, 216
Bst4CI ACNGT 1 cut(s) 886
Bst6I CTCTTC 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 702
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 5 cut(s) 147, 180, 193, 753, 808
BstDEI CTNAG 4 cut(s) 117, 126, 636, 648
BstF5I GGATG 4 cut(s) 281, 556, 560, 715
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 3 cut(s) 151, 306, 847
BstKTI GATC 3 cut(s) 92, 168, 622
BstMBI GATC 3 cut(s) 89, 165, 619
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 6 cut(s) 188, 209, 310, 338, 455, 844
BstNI CCWGG 2 cut(s) 153, 216
BstSCI CCNGG 2 cut(s) 151, 214
BstSFI CTRYAG 1 cut(s) 756
BstV1I GCAGC 7 cut(s) 190, 193, 196, 328, 460, 463, 613
BstX2I RGATCY 2 cut(s) 165, 619
BstYI RGATCY 2 cut(s) 165, 619
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 4 cut(s) 281, 556, 560, 715
Cac8I GCNNGC 5 cut(s) 147, 180, 193, 753, 808
CaiI CAGNNNCTG 1 cut(s) 206
CfoI GCGC 3 cut(s) 151, 306, 847
Cfr10I RCCGGY 1 cut(s) 178
CseI GACGC 3 cut(s) 413, 691, 764
CsiI ACCWGGT 1 cut(s) 214
CviAII CATG 2 cut(s) 98, 706
DdeI CTNAG 4 cut(s) 117, 126, 636, 648
DpnI GATC 3 cut(s) 91, 167, 621
DpnII GATC 3 cut(s) 89, 165, 619
DraIII CACNNNGTG 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 347
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 677
EcoRII CCWGG 2 cut(s) 151, 214
EcoT22I ATGCAT 2 cut(s) 608, 659
FaeI CATG 2 cut(s) 101, 709
FaqI GGGAC 1 cut(s) 886
FatI CATG 2 cut(s) 97, 705
FauI CCCGC 2 cut(s) 306, 863
Fnu4HI GCNGC 8 cut(s) 204, 207, 210, 342, 474, 477, 627, 868
FokI GGATG 4 cut(s) 268, 563, 567, 722
Fsp4HI GCNGC 8 cut(s) 204, 207, 210, 342, 474, 477, 627, 868
FspBI CTAG 2 cut(s) 459, 807
GlaI GCGC 3 cut(s) 150, 305, 846
GluI GCNGC 8 cut(s) 204, 207, 210, 342, 474, 477, 627, 868
GsuI CTGGAG 2 cut(s) 207, 452
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 179
HgaI GACGC 3 cut(s) 413, 691, 764
HhaI GCGC 3 cut(s) 151, 306, 847
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 2 cut(s) 101, 709
Hin6I GCGC 3 cut(s) 149, 304, 845
HinP1I GCGC 3 cut(s) 149, 304, 845
HinfI GANTC 5 cut(s) 83, 227, 350, 632, 646
HpaII CCGG 1 cut(s) 179
Hpy188I TCNGA 2 cut(s) 129, 574
Hpy188III TCNNGA 8 cut(s) 80, 87, 186, 560, 614, 650, 677, 679
HpyAV CCTTC 2 cut(s) 518, 777
HpyCH4III ACNGT 1 cut(s) 886
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 6 cut(s) 212, 344, 547, 606, 657, 751
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 209, 310, 338, 455, 844
HpyF3I CTNAG 4 cut(s) 117, 126, 636, 648
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 2 cut(s) 101, 709
HspAI GCGC 3 cut(s) 149, 304, 845
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
Kzo9I GATC 3 cut(s) 89, 165, 619
LmnI GCTCC 4 cut(s) 188, 406, 421, 835
Lsp1109I GCAGC 7 cut(s) 190, 193, 196, 328, 460, 463, 613
LweI GCATC 6 cut(s) 262, 290, 508, 666, 738, 808
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 2 cut(s) 459, 807
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 358
MalI GATC 3 cut(s) 91, 167, 621
MbiI CCGCTC 1 cut(s) 313
MboI GATC 3 cut(s) 89, 165, 619
MboII GAAGA 9 cut(s) 84, 119, 122, 236, 263, 301, 449, 629, 869
MflI RGATCY 2 cut(s) 165, 619
MluCI AATT 3 cut(s) 18, 51, 666
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 840
Mph1103I ATGCAT 2 cut(s) 608, 659
MroNI GCCGGC 1 cut(s) 178
MseI TTAA 3 cut(s) 50, 641, 771
MspA1I CMGCKG 3 cut(s) 203, 430, 867
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 153, 216
Mva1269I GAATGC 2 cut(s) 606, 659
MvaI CCWGG 2 cut(s) 153, 216
MwoI GCNNNNNNNGC 6 cut(s) 188, 209, 310, 338, 455, 844
NaeI GCCGGC 1 cut(s) 180
NdeII GATC 3 cut(s) 89, 165, 619
NgoMIV GCCGGC 1 cut(s) 178
NheI GCTAGC 1 cut(s) 806
NlaIII CATG 2 cut(s) 101, 709
NlaIV GGNNCC 2 cut(s) 167, 837
NsiI ATGCAT 2 cut(s) 608, 659
PaeR7I CTCGAG 1 cut(s) 677
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PctI GAATGC 2 cut(s) 606, 659
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 4 cut(s) 83, 227, 350, 632
PkrI GCNGC 8 cut(s) 205, 208, 211, 343, 475, 478, 628, 869
PleI GAGTC 1 cut(s) 640
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 1 cut(s) 640
Ppu21I YACGTR 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 151, 214
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 2 cut(s) 151, 214
PspN4I GGNNCC 2 cut(s) 167, 837
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 2 cut(s) 165, 619
PvuII CAGCTG 2 cut(s) 203, 430
SaqAI TTAA 3 cut(s) 50, 641, 771
SatI GCNGC 8 cut(s) 204, 207, 210, 342, 474, 477, 627, 868
Sau3AI GATC 3 cut(s) 89, 165, 619
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 2 cut(s) 153, 216
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 6 cut(s) 262, 290, 508, 666, 738, 808
SfcI CTRYAG 1 cut(s) 756
Sfr274I CTCGAG 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 3 cut(s) 18, 51, 666
SsiI CCGC 7 cut(s) 137, 156, 313, 373, 388, 867, 870
SspMI CTAG 2 cut(s) 459, 807
StyD4I CCNGG 2 cut(s) 151, 214
TaaI ACNGT 1 cut(s) 886
TaiI ACGT 1 cut(s) 349
TaqI TCGA 3 cut(s) 88, 664, 678
TaqII GACCGA 1 cut(s) 548
TasI AATT 3 cut(s) 18, 51, 666
TauI GCSGC 1 cut(s) 870
TfiI GAWTC 4 cut(s) 83, 227, 350, 632
Tru1I TTAA 3 cut(s) 50, 641, 771
Tru9I TTAA 3 cut(s) 50, 641, 771
TseI GCWGC 7 cut(s) 203, 206, 209, 341, 473, 476, 626
TspDTI ATGAA 3 cut(s) 114, 555, 814
TspGWI ACGGA 3 cut(s) 158, 220, 890
VspI ATTAAT 1 cut(s) 50
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XhoI CTCGAG 1 cut(s) 677
XspI CTAG 2 cut(s) 459, 807
Zsp2I ATGCAT 2 cut(s) 608, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.