AT4G27590

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006800
Physical Location & Seq
Forward (+)
13771089 .. 13772594
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27590.1

Sequence Viewer

Length: 471 bp
ATGGGTGATCTTCAAATCGTGCCGGTGTACAACAAGGTGGAAGCACAGTACGTAGAAATGATGGTGCCTTTGTATTCTTACGGTTGCGAGAAGAAAGTCAAGAGGGCTCTTTCTCATCTCAAAGGAATATATTCAGTGAAAGTAGACTACTACAATCAGAAGGTGACGGTGTGGGGGATTTGTAACAAACTCGATGTGTTAGCGATGGTGAAAAAGAAGCGAAAAGAAGCTCGGTTTTGGAATGTAGAGGAAGAGGAGAATAATAATCCAGAGAGCGTTGATGATTGCATTGTCGTAAAAGAAGACACTATTATTAAAACTTCATTTGATACCGATAAATCATCGGCGTTTTATACGTATTCTACAACGCCTCCGAGATTCAGTATAAGACCTCCTATGTCACTTATTAGAACCTCTTCATTCACATGGAAAGCCGTGAAGAAGGTCTTCACTAGATCCCTTTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

156

Amino Acids

18.13

Weight (kDa)

9.25

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 24 - 59 3.7e-08 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 1 cut(s) 144
AclWI GGATC 1 cut(s) 450
AfaI GTAC 2 cut(s) 29, 50
AgsI TTSAA 1 cut(s) 14
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
AlwI GGATC 1 cut(s) 450
Asp700I GAANNNNTTC 3 cut(s) 130, 415, 446
AsuHPI GGTGA 3 cut(s) 17, 175, 220
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 2 cut(s) 309, 439
BccI CCATC 2 cut(s) 55, 199
BceAI ACGGC 1 cut(s) 419
BfaI CTAG 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 66
BpiI GAAGAC 2 cut(s) 309, 439
BsaAI YACGTR 2 cut(s) 52, 357
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bse118I RCCGGY 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 269
BshNI GGYRCC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 7, 455
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 27
BssAI RCCGGY 1 cut(s) 22
BssMI GATC 2 cut(s) 7, 455
Bst4CI ACNGT 3 cut(s) 48, 83, 169
Bst6I CTCTTC 2 cut(s) 246, 421
BstAUI TGTACA 1 cut(s) 27
BstBAI YACGTR 2 cut(s) 52, 357
BstKTI GATC 2 cut(s) 10, 458
BstMBI GATC 2 cut(s) 7, 455
BstSNI TACGTA 2 cut(s) 52, 357
BstV2I GAAGAC 2 cut(s) 309, 439
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BtgZI GCGATG 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 22
Csp6I GTAC 2 cut(s) 28, 49
CviAII CATG 1 cut(s) 426
CviJI RGCY 3 cut(s) 107, 230, 434
CviKI_1 RGCY 3 cut(s) 107, 230, 434
CviQI GTAC 2 cut(s) 28, 49
DpnI GATC 2 cut(s) 9, 457
DpnII GATC 2 cut(s) 7, 455
Eam1104I CTCTTC 2 cut(s) 246, 421
EarI CTCTTC 2 cut(s) 246, 421
Eco105I TACGTA 2 cut(s) 52, 357
Eco24I GRGCYC 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 109
FaeI CATG 1 cut(s) 429
FaiI YATR 5 cut(s) 130, 354, 386, 398, 427
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 1 cut(s) 425
FblI GTMKAC 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 109
FspBI CTAG 1 cut(s) 453
HapII CCGG 1 cut(s) 23
Hin1II CATG 1 cut(s) 429
HinfI GANTC 1 cut(s) 378
HpaII CCGG 1 cut(s) 23
HphI GGTGA 3 cut(s) 17, 175, 220
Hpy166II GTNNAC 2 cut(s) 28, 145
Hpy188I TCNGA 2 cut(s) 159, 375
Hpy188III TCNNGA 2 cut(s) 100, 269
Hpy8I GTNNAC 2 cut(s) 28, 145
HpyAV CCTTC 2 cut(s) 154, 436
HpyCH4III ACNGT 3 cut(s) 48, 83, 169
HpyCH4IV ACGT 2 cut(s) 51, 356
HpyCH4V TGCA 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 51, 356
Hsp92II CATG 1 cut(s) 429
Kzo9I GATC 2 cut(s) 7, 455
LpnPI CCDG 2 cut(s) 36, 282
MaeI CTAG 1 cut(s) 453
MaeII ACGT 2 cut(s) 51, 356
MaeIII GTNAC 3 cut(s) 163, 182, 399
MalI GATC 2 cut(s) 9, 457
MboI GATC 2 cut(s) 7, 455
MboII GAAGA 6 cut(s) 103, 263, 314, 408, 439, 451
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 1 cut(s) 109
MnlI CCTC 6 cut(s) 96, 241, 247, 381, 402, 424
MroXI GAANNNNTTC 3 cut(s) 130, 415, 446
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 424
MspI CCGG 1 cut(s) 23
NdeII GATC 2 cut(s) 7, 455
NlaIII CATG 1 cut(s) 429
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 163, 399
PdmI GAANNNNTTC 3 cut(s) 130, 415, 446
PfeI GAWTC 1 cut(s) 378
Ppu21I YACGTR 2 cut(s) 52, 357
PspN4I GGNNCC 1 cut(s) 66
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 2 cut(s) 29, 50
RsaNI GTAC 2 cut(s) 28, 49
RseI CAYNNNNRTG 1 cut(s) 424
SaqAI TTAA 1 cut(s) 315
Sau3AI GATC 2 cut(s) 7, 455
SduI GDGCHC 1 cut(s) 109
SetI ASST 8 cut(s) 39, 54, 165, 232, 359, 394, 416, 447
SmiMI CAYNNNNRTG 1 cut(s) 424
SnaBI TACGTA 2 cut(s) 52, 357
SspMI CTAG 1 cut(s) 453
TaaI ACNGT 3 cut(s) 48, 83, 169
TaiI ACGT 2 cut(s) 54, 359
TaqI TCGA 1 cut(s) 192
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 2 cut(s) 163, 399
Tsp45I GTSAC 2 cut(s) 163, 399
TspDTI ATGAA 2 cut(s) 312, 408
TspRI CASTG 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 3 cut(s) 130, 415, 446
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.