MD01G1229600.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006800
Physical Location & Seq
Reverse (-)
31888774 .. 31889871
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1229600.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCCGATATGCAAATTGTTCCTGCCTGCAAAAACGTAGAGGCGCAGTATGTGGAGATGATGGTTCCTCTCTATTCTCATGGATGTGAGAAGAAAATAAAGAAGACCCTCTCCCATCTCAAAGGGATATACTCAGTGAAGGTGAATTATAACGAACAAAAGGTGACGGTATGGGGAATATGCAACAAATACGATGTGCTTGCAACTGTAAGGAGCAAGAGAAAACACGCATCTTTTTGGAACCCTGAAGACAACATTGCCTTAGAAGATCAATCACCAGAACCACCGGCGGTGACAACACCACCACCTTCCTCTCCTCCTTCTCCTATTCATAAGACTTCAAGTGGTAAATACACAAAGCCTTCTTTGGCTCTGATTAGGGTTCGATCTTTGAGCTGGAAAGCATGGACTCCATGGAAGAAAGTCTTCAATAGGTCCTATTCATTACCCTCAAGGCTGAAGCTCAACGTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

9.78

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 59 1.3e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AciI CCGC 1 cut(s) 290
AclI AACGTT 1 cut(s) 468
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 267
AgsI TTSAA 2 cut(s) 342, 430
AluBI AGCT 2 cut(s) 396, 463
AluI AGCT 2 cut(s) 396, 463
AoxI GGCC 1 cut(s) 3
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 4 cut(s) 154, 175, 267, 304
AvaII GGWCC 1 cut(s) 435
BbsI GAAGAC 3 cut(s) 110, 255, 418
BccI CCATC 2 cut(s) 55, 123
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 66, 242
BmsI GCATC 1 cut(s) 239
BpiI GAAGAC 3 cut(s) 110, 255, 418
BpuEI CTTGAG 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 413
Bse118I RCCGGY 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 306
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 287
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 268, 386
Bsp19I CCATGG 1 cut(s) 413
BspACI CCGC 1 cut(s) 290
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 66, 242
BsrDI GCAATG 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 2 cut(s) 268, 386
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 2 cut(s) 169, 208
BstC8I GCNNGC 2 cut(s) 28, 201
BstDEI CTNAG 2 cut(s) 133, 262
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 2 cut(s) 271, 389
BstMBI GATC 2 cut(s) 268, 386
BstV2I GAAGAC 3 cut(s) 110, 255, 418
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 89
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 28, 201
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 435
CviAII CATG 3 cut(s) 80, 405, 414
CviJI RGCY 6 cut(s) 5, 361, 371, 396, 457, 463
CviKI_1 RGCY 6 cut(s) 5, 361, 371, 396, 457, 463
DdeI CTNAG 2 cut(s) 133, 262
DpnI GATC 2 cut(s) 270, 388
DpnII GATC 2 cut(s) 268, 386
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 3 cut(s) 83, 408, 417
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 3 cut(s) 79, 404, 413
FokI GGATG 1 cut(s) 96
GlaI GCGC 1 cut(s) 45
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 287
HhaI GCGC 1 cut(s) 46
Hin1II CATG 3 cut(s) 83, 408, 417
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 1 cut(s) 409
HpaII CCGG 1 cut(s) 287
HphI GGTGA 4 cut(s) 154, 175, 267, 304
Hpy188I TCNGA 1 cut(s) 375
HpyAV CCTTC 4 cut(s) 133, 318, 330, 372
HpyCH4III ACNGT 2 cut(s) 169, 208
HpyCH4IV ACGT 2 cut(s) 36, 468
HpyCH4V TGCA 4 cut(s) 13, 30, 183, 203
HpyF3I CTNAG 2 cut(s) 133, 262
HpySE526I ACGT 2 cut(s) 36, 468
Hsp92II CATG 3 cut(s) 83, 408, 417
HspAI GCGC 1 cut(s) 44
Kzo9I GATC 2 cut(s) 268, 386
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 6 cut(s) 36, 40, 258, 291, 300, 382
LweI GCATC 1 cut(s) 239
MaeII ACGT 2 cut(s) 36, 468
MaeIII GTNAC 2 cut(s) 163, 292
MalI GATC 2 cut(s) 270, 388
MboI GATC 2 cut(s) 268, 386
MboII GAAGA 6 cut(s) 103, 115, 260, 278, 418, 430
MluCI AATT 3 cut(s) 15, 145, 472
MlyI GAGTC 1 cut(s) 403
MnlI CCTC 6 cut(s) 34, 78, 119, 322, 327, 460
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 84
MspI CCGG 1 cut(s) 287
NcoI CCATGG 1 cut(s) 413
NdeII GATC 2 cut(s) 268, 386
NlaIII CATG 3 cut(s) 83, 408, 417
NlaIV GGNNCC 2 cut(s) 66, 242
NmuCI GTSAC 2 cut(s) 163, 292
PdmI GAANNNNTTC 1 cut(s) 425
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 435
PsiI TTATAA 1 cut(s) 150
Psp1406I AACGTT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 66, 242
PspPI GGNCC 1 cut(s) 435
PspPPI RGGWCCY 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 1 cut(s) 471
Sau3AI GATC 2 cut(s) 268, 386
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 1 cut(s) 403
SetI ASST 8 cut(s) 39, 144, 165, 310, 398, 437, 465, 471
SfaNI GCATC 1 cut(s) 239
SgrAI CRCCGGYG 1 cut(s) 286
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 3 cut(s) 15, 145, 472
SsiI CCGC 1 cut(s) 290
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 2 cut(s) 169, 208
TaiI ACGT 2 cut(s) 39, 471
TaqI TCGA 1 cut(s) 385
TasI AATT 3 cut(s) 15, 145, 472
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 2 cut(s) 163, 292
Tsp45I GTSAC 2 cut(s) 163, 292
TspDTI ATGAA 2 cut(s) 320, 432
TspRI CASTG 1 cut(s) 141
VpaK11BI GGWCC 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.