Rroxscaffold_4G00277650

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006800
Physical Location & Seq
Forward (+)
986367 .. 987767
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277650.1

Sequence Viewer

Length: 453 bp
ATGGCTGATATGCAAATCGTTCTGGCTTTAAAAACTAGTGTGGAAGCGCAGTATGTGGAAATGATGGTTCCTCTTTATTCTCCTGGGTGCGAGAAGAAAGTCAAGAAGGCCCTATCCCACCTCAAAGGAATATACTCGGTGAACGTAGACTGCAGCCAACAAAAGGTGACGGTTTGGGGAATATGCAACAAATACGATGTGCTAGCCACAATAAGGACCAAGAGAAAACACGCTTGCTTTTGGAATCCTGAAGACAACGATATTGAAGAGGACGGAAAGGAAGCAGAGCTACCACCGACGGTGACGATGTCGGAGTCAGACGACTCAACACCACCGATTGCAAAAAGAAAGTACTTATTAAGACAGCCTTCTTTAAAGGCTCTCACCAGGGTTCGCTCTTTGAGCTTGAGAGCATGGAAGAAGGTCTTCACCAGGTCCCATTCATTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

150

Amino Acids

17.08

Weight (kDa)

9.28

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 24 - 60 6.2e-08 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 147
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 2 cut(s) 163, 213
AgsI TTSAA 1 cut(s) 266
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 3 cut(s) 82, 386, 431
AluBI AGCT 2 cut(s) 289, 405
AluI AGCT 2 cut(s) 289, 405
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 109, 216, 435
AsuHPI GGTGA 5 cut(s) 151, 178, 313, 376, 421
AsuNHI GCTAGC 1 cut(s) 202
AvaII GGWCC 2 cut(s) 216, 435
BarI GAAGNNNNNNTAC 2 cut(s) 273, 305
BbsI GAAGAC 2 cut(s) 258, 418
BbvI GCAGC 1 cut(s) 165
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 3 cut(s) 84, 388, 433
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 2 cut(s) 36, 203
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 1 cut(s) 154
BlsI GCNGC 1 cut(s) 155
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 3 cut(s) 84, 388, 433
Bme18I GGWCC 2 cut(s) 216, 435
BmgT120I GGNCC 3 cut(s) 109, 216, 435
BmiI GGNNCC 2 cut(s) 69, 437
BmrFI CCNGG 3 cut(s) 84, 388, 433
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 258, 418
BpuEI CTTGAG 1 cut(s) 427
BsaJI CCNNGG 2 cut(s) 83, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 213
BseBI CCWGG 3 cut(s) 84, 388, 433
BseDI CCNNGG 2 cut(s) 83, 387
BseLI CCNNNNNNNGG 2 cut(s) 163, 213
BseXI GCAGC 1 cut(s) 165
BshFI GGCC 1 cut(s) 110
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 2 cut(s) 163, 213
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 1 cut(s) 110
BspANI GGCC 1 cut(s) 110
BspHI TCATGA 1 cut(s) 449
BspLI GGNNCC 2 cut(s) 69, 437
BspMAI CTGCAG 1 cut(s) 155
BspOI GCTAGC 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 83, 387
Bst2UI CCWGG 3 cut(s) 84, 388, 433
Bst4CI ACNGT 2 cut(s) 172, 301
Bst6I CTCTTC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 204, 235
BstHHI GCGC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 3 cut(s) 84, 388, 433
BstSCI CCNGG 3 cut(s) 82, 386, 431
BstSFI CTRYAG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 2 cut(s) 258, 418
BsuRI GGCC 1 cut(s) 110
Cac8I GCNNGC 2 cut(s) 204, 235
CciI TCATGA 1 cut(s) 449
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 3 cut(s) 109, 216, 435
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 1 cut(s) 352
CviAII CATG 2 cut(s) 414, 450
CviJI RGCY 9 cut(s) 5, 26, 110, 156, 206, 289, 367, 380, 405
CviKI_1 RGCY 9 cut(s) 5, 26, 110, 156, 206, 289, 367, 380, 405
CviQI GTAC 1 cut(s) 352
DraI TTTAAA 2 cut(s) 30, 375
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 216, 435
Eco57I CTGAAG 1 cut(s) 270
EcoO109I RGGNCCY 2 cut(s) 109, 435
EcoRII CCWGG 3 cut(s) 82, 386, 431
FaeI CATG 2 cut(s) 417, 453
FaiI YATR 6 cut(s) 11, 54, 133, 184, 415, 451
FalI AAGNNNNNCTT 4 cut(s) 352, 384, 410, 442
FaqI GGGAC 1 cut(s) 421
FatI CATG 2 cut(s) 413, 449
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 154
Fsp4HI GCNGC 1 cut(s) 154
FspBI CTAG 2 cut(s) 36, 203
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 154
HaeIII GGCC 1 cut(s) 110
HhaI GCGC 1 cut(s) 49
Hin1II CATG 2 cut(s) 417, 453
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 3 cut(s) 244, 314, 323
HphI GGTGA 5 cut(s) 151, 178, 313, 376, 421
Hpy166II GTNNAC 2 cut(s) 142, 148
Hpy188I TCNGA 2 cut(s) 313, 319
Hpy188III TCNNGA 3 cut(s) 103, 248, 450
Hpy8I GTNNAC 2 cut(s) 142, 148
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 3 cut(s) 100, 378, 415
HpyCH4III ACNGT 2 cut(s) 172, 301
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 4 cut(s) 13, 153, 186, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 2 cut(s) 417, 453
HspAI GCGC 1 cut(s) 47
LpnPI CCDG 8 cut(s) 8, 69, 96, 261, 373, 400, 418, 445
Lsp1109I GCAGC 1 cut(s) 165
MabI ACCWGGT 1 cut(s) 431
MaeI CTAG 2 cut(s) 36, 203
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 166, 301
MboII GAAGA 5 cut(s) 106, 263, 278, 418, 430
MlyI GAGTC 2 cut(s) 317, 323
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 3 cut(s) 81, 131, 262
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 3 cut(s) 29, 359, 374
MslI CAYNNNNRTG 1 cut(s) 448
MspR9I CCNGG 3 cut(s) 84, 388, 433
MvaI CCWGG 3 cut(s) 84, 388, 433
MwoI GCNNNNNNNGC 1 cut(s) 402
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 2 cut(s) 417, 453
NlaIV GGNNCC 2 cut(s) 69, 437
NmuCI GTSAC 2 cut(s) 166, 301
PagI TCATGA 1 cut(s) 449
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 244
PflFI GACNNNGTC 1 cut(s) 307
PkrI GCNGC 1 cut(s) 155
PleI GAGTC 2 cut(s) 317, 322
PpsI GAGTC 2 cut(s) 317, 322
PpuMI RGGWCCY 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 82, 386, 431
PspGI CCWGG 3 cut(s) 82, 386, 431
PspN4I GGNNCC 2 cut(s) 69, 437
PspPI GGNCC 3 cut(s) 109, 216, 435
PspPPI RGGWCCY 1 cut(s) 435
PstI CTGCAG 1 cut(s) 155
PsyI GACNNNGTC 1 cut(s) 307
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 448
SaqAI TTAA 3 cut(s) 29, 359, 374
SatI GCNGC 1 cut(s) 154
Sau96I GGNCC 3 cut(s) 109, 216, 435
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 317, 323
ScrFI CCNGG 3 cut(s) 84, 388, 433
SetI ASST 7 cut(s) 123, 147, 168, 291, 407, 426, 437
SexAI ACCWGGT 1 cut(s) 431
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 2 cut(s) 216, 435
SmiMI CAYNNNNRTG 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
SpeI ACTAGT 1 cut(s) 35
SspMI CTAG 2 cut(s) 36, 203
StyD4I CCNGG 3 cut(s) 82, 386, 431
TaaI ACNGT 2 cut(s) 172, 301
TaiI ACGT 1 cut(s) 147
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 3 cut(s) 29, 359, 374
Tru9I TTAA 3 cut(s) 29, 359, 374
TseFI GTSAC 2 cut(s) 166, 301
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 2 cut(s) 166, 301
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 1 cut(s) 288
Tth111I GACNNNGTC 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 216, 435
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 36, 203
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.