Rh1CG434400

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006800
Physical Location & Seq
Reverse (-)
68979053 .. 68980016
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG434400.1

Sequence Viewer

Length: 534 bp
ATGCACAAATATAATCTGGAGTTTGTTAACTTGGCCGGAGTGAGTATTAGTTCTCTGCTGGTACTAGTTGGATTAATCATGGCTGATATGCAAATCGTTCTGGCTTTAAAAACTAGTGTGGAAGCGCAGTATGTGGAAATGATGGTTCCTCTTTATTCTCCTGGGTGCGAGAAGAAAGTCAAGAAGGCCCTATCCCACCTCAAAGGAATATACTCGGTGAACGTAGACTGCAGCCAACAAAAGGTGACGGTTTGGGGAATCTGCAACAAATACGATGTGCTAGCCACAATAAGGACCAAGAGAAAACACGCTTGCTTTTGGAATCCTGAAGACAACGATATTGAAGAGGACGGAGAGGAAGAAGCAGAGGTACCACCGACCGTGACGATGTCGGAGTCAGACGACTCAACACCACCGATTGCAAAAAGAAAGTACTTATTAAGACAGCCTTCTTTAAAGGCTCTCACCAGGGTTCGCTCTTTGAGCTTGAGAGCATGGAAGAAGGTCTTCACCAGGTCCCATTCATTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

177

Amino Acids

20.01

Weight (kDa)

8.83

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 50 - 86 8.5e-08 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 225
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 3 cut(s) 63, 372, 434
AfiI CCNNNNNNNGG 2 cut(s) 241, 291
AgsI TTSAA 1 cut(s) 344
AhlI ACTAGT 2 cut(s) 64, 113
AjnI CCWGG 3 cut(s) 160, 467, 512
AluBI AGCT 1 cut(s) 486
AluI AGCT 1 cut(s) 486
AoxI GGCC 2 cut(s) 33, 186
ApeKI GCWGC 1 cut(s) 231
AseI ATTAAT 1 cut(s) 74
Asp700I GAANNNNTTC 1 cut(s) 506
Asp718I GGTACC 1 cut(s) 370
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 187, 294, 516
AsuHPI GGTGA 4 cut(s) 229, 256, 457, 502
AsuNHI GCTAGC 1 cut(s) 280
AvaII GGWCC 2 cut(s) 294, 516
BanI GGYRCC 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 354, 386
BbsI GAAGAC 2 cut(s) 336, 499
BbvI GCAGC 1 cut(s) 243
BccI CCATC 1 cut(s) 136
BciT130I CCWGG 3 cut(s) 162, 469, 514
BcuI ACTAGT 2 cut(s) 64, 113
BfaI CTAG 3 cut(s) 65, 114, 281
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 3 cut(s) 162, 469, 514
Bme18I GGWCC 2 cut(s) 294, 516
BmgT120I GGNCC 3 cut(s) 187, 294, 516
BmiI GGNNCC 3 cut(s) 147, 372, 518
BmrFI CCNGG 3 cut(s) 162, 469, 514
BmtI GCTAGC 1 cut(s) 284
BpiI GAAGAC 2 cut(s) 336, 499
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 161, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 291
BseBI CCWGG 3 cut(s) 162, 469, 514
BseDI CCNNGG 2 cut(s) 161, 468
BseLI CCNNNNNNNGG 2 cut(s) 241, 291
BseXI GCAGC 1 cut(s) 243
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 2 cut(s) 35, 188
BshNI GGYRCC 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 381
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 2 cut(s) 241, 291
BsmFI GGGAC 1 cut(s) 502
BsnI GGCC 2 cut(s) 35, 188
BspANI GGCC 2 cut(s) 35, 188
BspHI TCATGA 1 cut(s) 530
BspLI GGNNCC 3 cut(s) 147, 372, 518
BspMAI CTGCAG 1 cut(s) 233
BspOI GCTAGC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 161, 468
Bst2UI CCWGG 3 cut(s) 162, 469, 514
Bst4CI ACNGT 2 cut(s) 250, 382
Bst6I CTCTTC 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 282, 313
BstHHI GCGC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 381
BstMWI GCNNNNNNNGC 1 cut(s) 483
BstNI CCWGG 3 cut(s) 162, 469, 514
BstSCI CCNGG 3 cut(s) 160, 467, 512
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 2 cut(s) 336, 499
BsuRI GGCC 2 cut(s) 35, 188
Cac8I GCNNGC 2 cut(s) 282, 313
CciI TCATGA 1 cut(s) 530
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 187, 294, 516
CsiI ACCWGGT 1 cut(s) 512
Csp6I GTAC 3 cut(s) 62, 371, 433
CviAII CATG 3 cut(s) 79, 495, 531
CviJI RGCY 9 cut(s) 35, 83, 104, 188, 234, 284, 448, 461, 486
CviKI_1 RGCY 9 cut(s) 35, 83, 104, 188, 234, 284, 448, 461, 486
CviQI GTAC 3 cut(s) 62, 371, 433
DraI TTTAAA 2 cut(s) 108, 456
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco47I GGWCC 2 cut(s) 294, 516
Eco57I CTGAAG 1 cut(s) 348
EcoO109I RGGNCCY 2 cut(s) 187, 516
EcoRII CCWGG 3 cut(s) 160, 467, 512
FaeI CATG 3 cut(s) 82, 498, 534
FaiI YATR 7 cut(s) 12, 80, 89, 132, 211, 496, 532
FalI AAGNNNNNCTT 4 cut(s) 433, 465, 491, 523
FaqI GGGAC 1 cut(s) 502
FatI CATG 3 cut(s) 78, 494, 530
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 3 cut(s) 65, 114, 281
GlaI GCGC 1 cut(s) 126
GluI GCNGC 1 cut(s) 232
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 35, 188
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 127
Hin1II CATG 3 cut(s) 82, 498, 534
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 4 cut(s) 258, 322, 395, 404
HpaI GTTAAC 1 cut(s) 28
HpaII CCGG 1 cut(s) 36
HphI GGTGA 4 cut(s) 229, 256, 457, 502
Hpy166II GTNNAC 3 cut(s) 28, 220, 226
Hpy188I TCNGA 2 cut(s) 394, 400
Hpy188III TCNNGA 4 cut(s) 17, 181, 326, 531
Hpy8I GTNNAC 3 cut(s) 28, 220, 226
HpyAV CCTTC 3 cut(s) 178, 459, 496
HpyCH4III ACNGT 2 cut(s) 250, 382
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 4, 91, 231, 264, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 483
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 3 cut(s) 82, 498, 534
HspAI GCGC 1 cut(s) 125
KpnI GGTACC 1 cut(s) 374
KspAI GTTAAC 1 cut(s) 28
Lsp1109I GCAGC 1 cut(s) 243
MabI ACCWGGT 1 cut(s) 512
MaeI CTAG 3 cut(s) 65, 114, 281
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 244, 382
MboII GAAGA 6 cut(s) 184, 341, 356, 371, 499, 511
MlyI GAGTC 2 cut(s) 398, 404
MmeI TCCRAC 2 cut(s) 49, 372
MnlI CCTC 5 cut(s) 159, 209, 340, 349, 361
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 5 cut(s) 27, 74, 107, 440, 455
MslI CAYNNNNRTG 1 cut(s) 529
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 162, 469, 514
MvaI CCWGG 3 cut(s) 162, 469, 514
MwoI GCNNNNNNNGC 1 cut(s) 483
NheI GCTAGC 1 cut(s) 280
NlaIII CATG 3 cut(s) 82, 498, 534
NlaIV GGNNCC 3 cut(s) 147, 372, 518
NmuCI GTSAC 2 cut(s) 244, 382
PagI TCATGA 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 258, 322
PflFI GACNNNGTC 1 cut(s) 388
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 2 cut(s) 398, 403
PpsI GAGTC 2 cut(s) 398, 403
PpuMI RGGWCCY 1 cut(s) 516
PshBI ATTAAT 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 516
Psp6I CCWGG 3 cut(s) 160, 467, 512
PspGI CCWGG 3 cut(s) 160, 467, 512
PspN4I GGNNCC 3 cut(s) 147, 372, 518
PspPI GGNCC 3 cut(s) 187, 294, 516
PspPPI RGGWCCY 1 cut(s) 516
PstI CTGCAG 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 3 cut(s) 63, 372, 434
RsaNI GTAC 3 cut(s) 62, 371, 433
RseI CAYNNNNRTG 1 cut(s) 529
SaqAI TTAA 5 cut(s) 27, 74, 107, 440, 455
SatI GCNGC 1 cut(s) 232
Sau96I GGNCC 3 cut(s) 187, 294, 516
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 398, 404
ScrFI CCNGG 3 cut(s) 162, 469, 514
SetI ASST 7 cut(s) 201, 225, 246, 372, 488, 507, 518
SexAI ACCWGGT 1 cut(s) 512
SfcI CTRYAG 1 cut(s) 229
SinI GGWCC 2 cut(s) 294, 516
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
SpeI ACTAGT 2 cut(s) 64, 113
SspMI CTAG 3 cut(s) 65, 114, 281
StyD4I CCNGG 3 cut(s) 160, 467, 512
TaaI ACNGT 2 cut(s) 250, 382
TaiI ACGT 1 cut(s) 225
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 2 cut(s) 258, 322
Tru1I TTAA 5 cut(s) 27, 74, 107, 440, 455
Tru9I TTAA 5 cut(s) 27, 74, 107, 440, 455
TseFI GTSAC 2 cut(s) 244, 382
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 2 cut(s) 244, 382
TspDTI ATGAA 1 cut(s) 513
TspGWI ACGGA 1 cut(s) 366
Tth111I GACNNNGTC 1 cut(s) 388
VpaK11BI GGWCC 2 cut(s) 294, 516
VspI ATTAAT 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 3 cut(s) 65, 114, 281
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.