pycom07g27430

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006800
Physical Location & Seq
Reverse (-)
27177171 .. 27178169
999 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27430.1

Sequence Viewer

Length: 507 bp
ATGGCCGATATGCAAATCGTTCCCGCCTGCAAAAACGTAGAGGCGCAGTATGTGGAGATGATGGTTCCTCTCTATTCTCATGGATGTGAGAAGAAAATAGAGAAGACCCTCTCCCATCTCAAAGGGATATACTCAGTGAAGGTGGATTATAACGAACAAAAGGTGACGGTATGGGGAATATGCAACAAATATGATGTGCTTGCAACAGTAAGGAGCAAGAGAAAACACTCATGTTTTTGGAACTCTGAAGACAACATTGCCTTAGAAGATCAATCAGCAGAACCACCGTCAGTGACAACACCACCACCTTGCTCTCCCCCTCCTCCCATTACTACTCTTTCAAGTTTTAAATACACAAAGCCTTCTTTGGCTCTGATTAAGGTTCGATCTTTGAGTTGGAAAGCATGGACTCCATGGAAAAAAGTCTTCAATAGGTCCTATTCATTACCCTCAAGGCTGAAGCTCAACGTGAATGGATTATTTGTAACGATCGATAAGAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

169

Amino Acids

19.14

Weight (kDa)

9.42

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 59 1.1e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AciI CCGC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 267, 479
AgsI TTSAA 2 cut(s) 342, 430
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
AoxI GGCC 1 cut(s) 3
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 435
BbsI GAAGAC 3 cut(s) 110, 255, 418
BccI CCATC 2 cut(s) 55, 123
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 66
BpiI GAAGAC 3 cut(s) 110, 255, 418
BpuEI CTTGAG 1 cut(s) 436
Bsa29I ATCGAT 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 492
BsiEI CGRYCG 1 cut(s) 492
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 268, 386, 489
Bsp19I CCATGG 1 cut(s) 413
BspACI CCGC 1 cut(s) 24
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 146
BspDI ATCGAT 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 3 cut(s) 268, 386, 489
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 3 cut(s) 169, 208, 288
BstC8I GCNNGC 2 cut(s) 28, 201
BstDEI CTNAG 2 cut(s) 133, 262
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 3 cut(s) 271, 389, 492
BstMBI GATC 3 cut(s) 268, 386, 489
BstMCI CGRYCG 1 cut(s) 492
BstV2I GAAGAC 3 cut(s) 110, 255, 418
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 89
BtsIMutI CAGTG 2 cut(s) 141, 297
Cac8I GCNNGC 2 cut(s) 28, 201
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 435
ClaI ATCGAT 1 cut(s) 492
CviAII CATG 4 cut(s) 80, 231, 405, 414
CviJI RGCY 5 cut(s) 5, 361, 371, 457, 463
CviKI_1 RGCY 5 cut(s) 5, 361, 371, 457, 463
DdeI CTNAG 2 cut(s) 133, 262
DpnI GATC 3 cut(s) 270, 388, 491
DpnII GATC 3 cut(s) 268, 386, 489
DraI TTTAAA 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 435
Eco57I CTGAAG 2 cut(s) 267, 479
EcoO109I RGGNCCY 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 4 cut(s) 83, 234, 408, 417
FatI CATG 4 cut(s) 79, 230, 404, 413
FauI CCCGC 1 cut(s) 31
FokI GGATG 1 cut(s) 96
GlaI GCGC 1 cut(s) 45
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 46
Hin1II CATG 4 cut(s) 83, 234, 408, 417
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 1 cut(s) 409
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 247, 375
HpyAV CCTTC 2 cut(s) 133, 372
HpyCH4III ACNGT 3 cut(s) 169, 208, 288
HpyCH4IV ACGT 2 cut(s) 36, 468
HpyCH4V TGCA 4 cut(s) 13, 30, 183, 203
HpyF3I CTNAG 2 cut(s) 133, 262
HpySE526I ACGT 2 cut(s) 36, 468
Hsp92II CATG 4 cut(s) 83, 234, 408, 417
HspAI GCGC 1 cut(s) 44
Kzo9I GATC 3 cut(s) 268, 386, 489
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 1 cut(s) 40
MaeII ACGT 2 cut(s) 36, 468
MaeIII GTNAC 3 cut(s) 163, 292, 484
MalI GATC 3 cut(s) 270, 388, 491
MboI GATC 3 cut(s) 268, 386, 489
MboII GAAGA 5 cut(s) 103, 115, 260, 278, 418
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 6 cut(s) 34, 78, 119, 330, 333, 460
MseI TTAA 2 cut(s) 348, 378
MslI CAYNNNNRTG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 413
NdeII GATC 3 cut(s) 268, 386, 489
NlaIII CATG 4 cut(s) 83, 234, 408, 417
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 163, 292
Ple19I CGATCG 1 cut(s) 492
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 435
PsiI TTATAA 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 435
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 435
PspPPI RGGWCCY 1 cut(s) 435
PvuI CGATCG 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 2 cut(s) 348, 378
Sau3AI GATC 3 cut(s) 268, 386, 489
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 1 cut(s) 403
SetI ASST 8 cut(s) 39, 144, 165, 310, 384, 437, 465, 471
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
SsiI CCGC 1 cut(s) 24
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 3 cut(s) 169, 208, 288
TaiI ACGT 2 cut(s) 39, 471
TaqI TCGA 2 cut(s) 385, 492
Tru1I TTAA 2 cut(s) 348, 378
Tru9I TTAA 2 cut(s) 348, 378
TscAI CASTG 2 cut(s) 141, 297
TseFI GTSAC 2 cut(s) 163, 292
Tsp45I GTSAC 2 cut(s) 163, 292
TspDTI ATGAA 1 cut(s) 432
TspRI CASTG 2 cut(s) 141, 297
VpaK11BI GGWCC 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.