MD07G1301600.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006800
Physical Location & Seq
Reverse (-)
36024780 .. 36025986
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1301600.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCCGATATGCAAATCGTTCCCGCCTGCAAAAACGTAGAGGCGCAGTATGTGGAGATGATGGTTCCTCTCTATTCTCATGGATGTGAGAAGAAAATAGAGAAGACCCTCTCCCATCTCAAAGGGATATACTCAGTGAAGGTGGATTATAACGAACAAAAGGTGACGGTATGGGGAATATGCAACAAATACGATGTGCTTGCAACTGTAAGGAGCAAGAGAAAACATGCATGTTTTTGGAACTCTGAAGACAACATTGCCTTAGAAGATCAATCACCAGAACCACTGTCAGTGACAACACCACCACCTTCCTCTCCTCCTCCTCCCATTACTACTCTTTCAAGTTTTAAATACACAAAGCCTTCTTTGGCTCTGATTAGGGTTCGATCTTTGAGTTGGAAAGCATGGACTCCATGGAAAAAAGTCTTTAATTGGTCCTATTCATTACCCTCAAGGCTGAAGCTCAACGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

159

Amino Acids

18.07

Weight (kDa)

9.35

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 59 9.9e-10 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AciI CCGC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 267
AgsI TTSAA 1 cut(s) 342
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
AoxI GGCC 1 cut(s) 3
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 2 cut(s) 175, 267
AvaII GGWCC 1 cut(s) 435
BbsI GAAGAC 2 cut(s) 110, 255
BccI CCATC 2 cut(s) 55, 123
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 66
BpiI GAAGAC 2 cut(s) 110, 255
BpuEI CTTGAG 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 3 cut(s) 306, 309, 312
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 268, 386
Bsp19I CCATGG 1 cut(s) 413
BspACI CCGC 1 cut(s) 24
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 2 cut(s) 268, 386
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 3 cut(s) 169, 208, 288
BstC8I GCNNGC 2 cut(s) 28, 201
BstDEI CTNAG 2 cut(s) 133, 262
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 2 cut(s) 271, 389
BstMBI GATC 2 cut(s) 268, 386
BstNSI RCATGY 2 cut(s) 230, 234
BstV2I GAAGAC 2 cut(s) 110, 255
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 89
BtsIMutI CAGTG 3 cut(s) 141, 284, 297
Cac8I GCNNGC 2 cut(s) 28, 201
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 435
CviAII CATG 5 cut(s) 80, 227, 231, 405, 414
CviJI RGCY 5 cut(s) 5, 361, 371, 457, 463
CviKI_1 RGCY 5 cut(s) 5, 361, 371, 457, 463
DdeI CTNAG 2 cut(s) 133, 262
DpnI GATC 2 cut(s) 270, 388
DpnII GATC 2 cut(s) 268, 386
DraI TTTAAA 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 413
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 5 cut(s) 83, 230, 234, 408, 417
FatI CATG 5 cut(s) 79, 226, 230, 404, 413
FauI CCCGC 1 cut(s) 31
FokI GGATG 1 cut(s) 96
GlaI GCGC 1 cut(s) 45
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 46
Hin1II CATG 5 cut(s) 83, 230, 234, 408, 417
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 1 cut(s) 409
HphI GGTGA 2 cut(s) 175, 267
Hpy188I TCNGA 2 cut(s) 247, 375
HpyAV CCTTC 3 cut(s) 133, 318, 372
HpyCH4III ACNGT 3 cut(s) 169, 208, 288
HpyCH4IV ACGT 2 cut(s) 36, 468
HpyCH4V TGCA 5 cut(s) 13, 30, 183, 203, 230
HpyF3I CTNAG 2 cut(s) 133, 262
HpySE526I ACGT 2 cut(s) 36, 468
Hsp92II CATG 5 cut(s) 83, 230, 234, 408, 417
HspAI GCGC 1 cut(s) 44
Kzo9I GATC 2 cut(s) 268, 386
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 2 cut(s) 40, 291
MaeII ACGT 2 cut(s) 36, 468
MaeIII GTNAC 2 cut(s) 163, 292
MalI GATC 2 cut(s) 270, 388
MboI GATC 2 cut(s) 268, 386
MboII GAAGA 4 cut(s) 103, 115, 260, 278
MluCI AATT 2 cut(s) 430, 472
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 8 cut(s) 34, 78, 119, 322, 327, 330, 333, 460
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 2 cut(s) 348, 429
MslI CAYNNNNRTG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 413
NdeII GATC 2 cut(s) 268, 386
NlaIII CATG 5 cut(s) 83, 230, 234, 408, 417
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 163, 292
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 2 cut(s) 230, 234
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PsiI TTATAA 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 2 cut(s) 348, 429
Sau3AI GATC 2 cut(s) 268, 386
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 1 cut(s) 403
SetI ASST 6 cut(s) 39, 144, 165, 310, 465, 471
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 2 cut(s) 430, 472
SsiI CCGC 1 cut(s) 24
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 3 cut(s) 169, 208, 288
TaiI ACGT 2 cut(s) 39, 471
TaqI TCGA 1 cut(s) 385
TasI AATT 2 cut(s) 430, 472
Tru1I TTAA 2 cut(s) 348, 429
Tru9I TTAA 2 cut(s) 348, 429
TscAI CASTG 3 cut(s) 141, 291, 297
TseFI GTSAC 2 cut(s) 163, 292
Tsp45I GTSAC 2 cut(s) 163, 292
TspDTI ATGAA 1 cut(s) 432
TspRI CASTG 3 cut(s) 141, 291, 297
VpaK11BI GGWCC 1 cut(s) 435
XceI RCATGY 2 cut(s) 230, 234
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.