AT4G28640

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009009
Physical Location & Seq
Forward (+)
14142063 .. 14143969
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28640.3

Sequence Viewer

Length: 741 bp
ATGGAAGGCGGTTCCGCTAGTGGATCGGCTTCGGCTTTGTCAAACGATGAAAATCTCGTCGTTTCTTGTGAGGATTCTTCTTCTCCTATAGGGAATGAGCTTGAGCTTGGTCTTACGTTGAGCCTTGGTCGCAAAGGGTATCGAGATTGTAGGGTTTACGCTGATGATTCTTCTTCTTCTTCATCATCTTCTTCTCTGAGCAGAGCTAGCGTAATTGCTGGGATCAAGAGGACAGCTGATTCCATGGCTGCAACTAGTGGGCAAGTTGTGGGATGGCCACCAATAAGGACTTACAGAATGAACAGTATGGTTAACCAAGCTAAGGCTTCAGCCACTGAAGATCCGAACTTGGAGATAAGTCAAGCCGTAAACAAGAATAGAAGTGATTCAACAAAGATGAGAAATTCCATGTTTGTTAAGGTGACTATGGATGGCATTCCTATTGGAAGGAAAATCGATCTGAATGCTCATAAATGCTATGAATCATTGTCAAACACTCTAGAGGAAATGTTTCTGAAACCTAAATTAGGTTCACGCACACTAGAAACCGATGGTCACATGGAAACACCGGTCAAGATACTACCAGATGGGTCTTCCGGATTAGTACTAACGTATGAAGACAAGGAAGGAGATTGGATGCTTGTTGGCGATGTTCCGTGGGGGATGTTTATTGGTTCAGTGAGAAGGCTCCGGATAATGAAAACATCGGAGGCTACTGGTAAAGCTCAGATGATATTATGA

Protein Analysis

246

Amino Acids

26.52

Weight (kDa)

5.82

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 58 - 240 6.9e-59 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 596, 690
AciI CCGC 2 cut(s) 9, 15
AclWI GGATC 3 cut(s) 31, 230, 335
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 2 cut(s) 312, 357
AfaI GTAC 1 cut(s) 606
AfiI CCNNNNNNNGG 2 cut(s) 322, 527
AgeI ACCGGT 1 cut(s) 568
AgsI TTSAA 1 cut(s) 390
AhlI ACTAGT 1 cut(s) 254
AluBI AGCT 6 cut(s) 100, 106, 206, 236, 320, 725
AluI AGCT 6 cut(s) 100, 106, 206, 236, 320, 725
AlwI GGATC 3 cut(s) 31, 230, 335
AlwNI CAGNNNCTG 1 cut(s) 335
Aor13HI TCCGGA 2 cut(s) 596, 690
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 403
AsiGI ACCGGT 1 cut(s) 568
Asp700I GAANNNNTTC 2 cut(s) 385, 510
AsuHPI GGTGA 1 cut(s) 433
AsuNHI GCTAGC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 585, 624
BbvI GCAGC 1 cut(s) 235
BccI CCATC 4 cut(s) 267, 425, 545, 581
BceAI ACGGC 1 cut(s) 350
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 5 cut(s) 18, 207, 255, 500, 542
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 606
BmiI GGNNCC 2 cut(s) 13, 689
BmsI GCATC 1 cut(s) 627
BmtI GCTAGC 1 cut(s) 210
BpiI GAAGAC 2 cut(s) 585, 624
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 124, 243, 656
BsaWI WCCGGW 3 cut(s) 568, 596, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 527
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 1 cut(s) 721
Bse8I GATNNNNATC 1 cut(s) 51
BseAI TCCGGA 2 cut(s) 596, 690
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 124, 243, 656
BseGI GGATG 4 cut(s) 278, 436, 642, 669
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 322, 527
BseMII CTCAG 2 cut(s) 188, 740
BseNI ACTGG 1 cut(s) 721
BseXI GCAGC 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 218
BshFI GGCC 1 cut(s) 277
BshTI ACCGGT 1 cut(s) 568
BshVI ATCGAT 1 cut(s) 456
BsiSI CCGG 3 cut(s) 569, 597, 691
BslI CCNNNNNNNGG 2 cut(s) 322, 527
BsmI GAATGC 2 cut(s) 435, 469
BsnI GGCC 1 cut(s) 277
Bsp13I TCCGGA 2 cut(s) 596, 690
Bsp143I GATC 4 cut(s) 23, 222, 340, 457
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 2 cut(s) 9, 15
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 189, 739
BspDI ATCGAT 1 cut(s) 456
BspEI TCCGGA 2 cut(s) 596, 690
BspLI GGNNCC 2 cut(s) 13, 689
BspOI GCTAGC 1 cut(s) 210
BspPI GGATC 3 cut(s) 31, 230, 335
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 1 cut(s) 721
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 124, 243, 656
BssMI GATC 4 cut(s) 23, 222, 340, 457
BssT1I CCWWGG 2 cut(s) 124, 243
Bst4CI ACNGT 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 3 cut(s) 197, 321, 726
BstDSI CCRYGG 2 cut(s) 243, 656
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 4 cut(s) 278, 436, 642, 669
BstKTI GATC 4 cut(s) 26, 225, 343, 460
BstMBI GATC 4 cut(s) 23, 222, 340, 457
BstMWI GCNNNNNNNGC 2 cut(s) 129, 207
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 2 cut(s) 585, 624
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 456
BsuRI GGCC 1 cut(s) 277
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 2 cut(s) 243, 656
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 4 cut(s) 278, 436, 642, 669
BtsIMutI CAGTG 2 cut(s) 333, 684
Cac8I GCNNGC 1 cut(s) 208
CaiI CAGNNNCTG 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 568
ClaI ATCGAT 1 cut(s) 456
Csp6I GTAC 1 cut(s) 605
CspAI ACCGGT 1 cut(s) 568
CviAII CATG 3 cut(s) 244, 409, 559
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 3 cut(s) 197, 321, 726
DpnI GATC 4 cut(s) 25, 224, 342, 459
DpnII GATC 4 cut(s) 23, 222, 340, 457
EaeI YGGCCR 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 124, 243
Eco57I CTGAAG 2 cut(s) 312, 357
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoT14I CCWWGG 2 cut(s) 124, 243
ErhI CCWWGG 2 cut(s) 124, 243
FaeI CATG 3 cut(s) 247, 412, 562
FatI CATG 3 cut(s) 243, 408, 558
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 4 cut(s) 285, 443, 649, 676
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 5 cut(s) 18, 207, 255, 500, 542
GluI GCNGC 1 cut(s) 249
GsaI CCCAGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 277
HapII CCGG 3 cut(s) 569, 597, 691
Hin1II CATG 3 cut(s) 247, 412, 562
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 5 cut(s) 74, 167, 239, 386, 482
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 3 cut(s) 569, 597, 691
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 4 cut(s) 157, 313, 370, 533
Hpy188I TCNGA 6 cut(s) 198, 345, 462, 516, 709, 729
Hpy188III TCNNGA 6 cut(s) 143, 226, 500, 574, 597, 691
Hpy8I GTNNAC 4 cut(s) 157, 313, 370, 533
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 441, 620, 678
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 116, 611
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 207
HpyF3I CTNAG 3 cut(s) 197, 321, 726
HpySE526I ACGT 2 cut(s) 116, 611
Hsp92II CATG 3 cut(s) 247, 412, 562
Kpn2I TCCGGA 2 cut(s) 596, 690
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 23, 222, 340, 457
LmnI GCTCC 1 cut(s) 693
LpnPI CCDG 6 cut(s) 204, 582, 597, 610, 702, 704
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 1 cut(s) 627
MaeI CTAG 5 cut(s) 18, 207, 255, 500, 542
MaeII ACGT 2 cut(s) 116, 611
MaeIII GTNAC 2 cut(s) 421, 554
MalI GATC 4 cut(s) 25, 224, 342, 459
MboI GATC 4 cut(s) 23, 222, 340, 457
MflI RGATCY 1 cut(s) 340
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 3 cut(s) 213, 403, 524
MluNI TGGCCA 1 cut(s) 277
MnlI CCTC 4 cut(s) 64, 222, 496, 703
Mox20I TGGCCA 1 cut(s) 277
MroI TCCGGA 2 cut(s) 596, 690
MroXI GAANNNNTTC 2 cut(s) 385, 510
MscI TGGCCA 1 cut(s) 277
MseI TTAA 2 cut(s) 312, 417
Msp20I TGGCCA 1 cut(s) 277
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 569, 597, 691
Mva1269I GAATGC 2 cut(s) 435, 469
MwoI GCNNNNNNNGC 2 cut(s) 129, 207
NcoI CCATGG 1 cut(s) 243
NdeII GATC 4 cut(s) 23, 222, 340, 457
NheI GCTAGC 1 cut(s) 206
NlaIII CATG 3 cut(s) 247, 412, 562
NlaIV GGNNCC 2 cut(s) 13, 689
NmuCI GTSAC 2 cut(s) 421, 554
PctI GAATGC 2 cut(s) 435, 469
PdmI GAANNNNTTC 2 cut(s) 385, 510
PfeI GAWTC 5 cut(s) 74, 167, 239, 386, 482
PinAI ACCGGT 1 cut(s) 568
PkrI GCNGC 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 13, 689
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
SaqAI TTAA 2 cut(s) 312, 417
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 4 cut(s) 23, 222, 340, 457
ScaI AGTACT 1 cut(s) 606
SfaNI GCATC 1 cut(s) 627
SfcI CTRYAG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 3 cut(s) 213, 403, 524
SsiI CCGC 2 cut(s) 9, 15
SspMI CTAG 5 cut(s) 18, 207, 255, 500, 542
StyI CCWWGG 2 cut(s) 124, 243
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 2 cut(s) 119, 614
TaqI TCGA 2 cut(s) 142, 456
TasI AATT 3 cut(s) 213, 403, 524
TatI WGTACW 1 cut(s) 604
TfiI GAWTC 5 cut(s) 74, 167, 239, 386, 482
Tru1I TTAA 2 cut(s) 312, 417
Tru9I TTAA 2 cut(s) 312, 417
TscAI CASTG 2 cut(s) 340, 684
TseFI GTSAC 2 cut(s) 421, 554
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 2 cut(s) 421, 554
TspDTI ATGAA 6 cut(s) 63, 171, 314, 495, 630, 713
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 2 cut(s) 340, 684
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 1 cut(s) 403
XbaI TCTAGA 1 cut(s) 499
XmnI GAANNNNTTC 2 cut(s) 385, 510
XspI CTAG 5 cut(s) 18, 207, 255, 500, 542
ZrmI AGTACT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.