FvH4_2g20500

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009009
Physical Location & Seq
Reverse (-)
17197666 .. 17200698
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20500.t2

Sequence Viewer

Length: 897 bp
ATGCCTTCAGCAACTGGGTCTGTCACTGACATGTCGATTGTGTCTAAAGATGACAACTTGTCTTCCGAGGATTCTTGTAACCCAGATGAGTCTGAGCTCGAGCTCAATCTTGGCCTCAGCCTCGGCGGCGGTGCTGCCAAGGCCCACCAAGGCCCAATTGGGAGCTATGCGAGGATTTTGACGGCTAAGGATTTCCGGTATGTGGGTTTGAACAAGTCGGATAAGTCGTCGGCGGAGGTTGGTTCTTCGTCGTCGTCGTCTTCTTCTTCGTTGAGCAGTGTTAATGTGGCGGCTGGGACTAAGCGGAGTGCTGATTCTGTTGCCAACAGTCAGCCTGTGGGATGGCCTCCTATCAGAACTTATAGAATGAATTCTTTGGTTCATCAAGCAAAATCTTCAGCCACTGAAGTAGTTAACTCAGAGAAAGGTGAATGCAAGATTGTTTCAGGGAAGGCTAAAATCGGTGGTCAAGAAAACAATGGAAATGCTAAGGAGAGACAGCGCCTCACAAGTTCCCTGTTTGTAAAGGTGAATATGGATGGAAGTCCAATTGGAAGGAAAGTCAACCTAACTGCGCACAGTAGCTATGAAGCCCTAGCACAAACGCTGGAGGATATGTTCCAGGGACCGTCTTCAAATGCGAAATTGATACGTGCTGGTGGTCAGGAGCATGATATGATGGTGGGAGAAACTACACCGTCGAAGTTGCTTGATGGATCATTTGATTTTGCACTCACTTATGAAGACAAAGAAGGTGACTGGATGCTTGTTGGGGATGTTCCTTGGGGGATGTTTCTTAGTTCCGTGAAGAGGCTAAGAATCATGAGGACTTCTGAAGCTAATGGACTTGCTCCAAGGCTACAGGAAAGGAATGCAAGGCAGAGATGCACGAGCTGA

Protein Analysis

299

Amino Acids

31.79

Weight (kDa)

6.92

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 33 - 285 5.3e-62 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 576
AciI CCGC 5 cut(s) 126, 129, 233, 290, 304
AclWI GGATC 1 cut(s) 724
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 3 cut(s) 381, 426, 855
AfiI CCNNNNNNNGG 2 cut(s) 202, 810
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 211, 636
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 621
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 6 cut(s) 97, 103, 165, 585, 839, 894
AluI AGCT 6 cut(s) 97, 103, 165, 585, 839, 894
Alw21I GWGCWC 2 cut(s) 99, 105
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 1 cut(s) 724
AlwNI CAGNNNCTG 2 cut(s) 14, 404
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 4 cut(s) 112, 141, 151, 344
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 370
AspLEI GCGC 2 cut(s) 504, 577
AspS9I GGNCC 3 cut(s) 142, 152, 626
AsuHPI GGTGA 3 cut(s) 440, 541, 767
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 99, 105
BauI CACGAG 1 cut(s) 889
BbsI GAAGAC 4 cut(s) 54, 252, 624, 750
Bbv12I GWGCWC 2 cut(s) 99, 105
BbvCI CCTCAGC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 121
BccI CCATC 4 cut(s) 336, 533, 673, 707
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 623
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 1 cut(s) 596
BfmI CTRYAG 1 cut(s) 860
BfoI RGCGCY 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 126
BisI GCNGC 3 cut(s) 127, 135, 291
BlsI GCNGC 3 cut(s) 128, 136, 292
Bme1390I CCNGG 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 626
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 142, 152, 626
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 623
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 753, 875
BmuI ACTGGG 1 cut(s) 24
BpiI GAAGAC 4 cut(s) 54, 252, 624, 750
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 3 cut(s) 116, 186, 489
BsaAI YACGTR 1 cut(s) 653
BsaJI CCNNGG 7 cut(s) 66, 121, 138, 148, 622, 782, 854
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 810
Bse1I ACTGG 2 cut(s) 19, 764
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 7 cut(s) 66, 121, 138, 148, 622, 782, 854
BseGI GGATG 5 cut(s) 347, 544, 768, 781, 795
BseLI CCNNNNNNNGG 2 cut(s) 202, 810
BseMII CTCAG 3 cut(s) 84, 130, 432
BseNI ACTGG 2 cut(s) 19, 764
BseXI GCAGC 1 cut(s) 121
BseYI CCCAGC 1 cut(s) 293
BshFI GGCC 4 cut(s) 114, 143, 153, 346
BsiHKAI GWGCWC 2 cut(s) 99, 105
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 196
BslFI GGGAC 2 cut(s) 310, 639
BslI CCNNNNNNNGG 2 cut(s) 202, 810
BsmAI GTCTC 1 cut(s) 490
BsmFI GGGAC 2 cut(s) 310, 639
BsmI GAATGC 2 cut(s) 437, 877
BsnI GGCC 4 cut(s) 114, 143, 153, 346
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 99, 105
Bsp143I GATC 1 cut(s) 716
BspACI CCGC 5 cut(s) 126, 129, 233, 290, 304
BspANI GGCC 4 cut(s) 114, 143, 153, 346
BspCNI CTCAG 3 cut(s) 85, 129, 431
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 1 cut(s) 627
BspPI GGATC 1 cut(s) 724
BsrI ACTGG 2 cut(s) 19, 764
BssECI CCNNGG 7 cut(s) 66, 121, 138, 148, 622, 782, 854
BssMI GATC 1 cut(s) 716
BssSI CACGAG 1 cut(s) 889
BssT1I CCWWGG 4 cut(s) 138, 148, 782, 854
Bst2BI CACGAG 1 cut(s) 889
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 4 cut(s) 329, 581, 630, 699
Bst6I CTCTTC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 653
BstDEI CTNAG 8 cut(s) 93, 116, 186, 300, 418, 489, 797, 815
BstF5I GGATG 5 cut(s) 347, 544, 768, 781, 795
BstH2I RGCGCY 1 cut(s) 505
BstHHI GCGC 2 cut(s) 504, 577
BstKTI GATC 1 cut(s) 719
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 1 cut(s) 716
BstMWI GCNNNNNNNGC 2 cut(s) 126, 140
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 621
BstSFI CTRYAG 1 cut(s) 860
BstV1I GCAGC 1 cut(s) 121
BstV2I GAAGAC 4 cut(s) 54, 252, 624, 750
BsuRI GGCC 4 cut(s) 114, 143, 153, 346
BtsCI GGATG 5 cut(s) 347, 544, 768, 781, 795
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 3 cut(s) 24, 283, 402
CaiI CAGNNNCTG 2 cut(s) 14, 404
CciI TCATGA 1 cut(s) 822
CfoI GCGC 2 cut(s) 504, 577
Cfr13I GGNCC 3 cut(s) 142, 152, 626
CviAII CATG 3 cut(s) 31, 671, 823
DdeI CTNAG 8 cut(s) 93, 116, 186, 300, 418, 489, 797, 815
DpnI GATC 1 cut(s) 718
DpnII GATC 1 cut(s) 716
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 803
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 803
EciI GGCGGA 1 cut(s) 248
Ecl136II GAGCTC 2 cut(s) 97, 103
Eco130I CCWWGG 4 cut(s) 138, 148, 782, 854
Eco24I GRGCYC 2 cut(s) 99, 105
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 2 cut(s) 97, 103
Eco57I CTGAAG 3 cut(s) 381, 426, 855
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 2 cut(s) 97, 103
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 4 cut(s) 138, 148, 782, 854
EcoT38I GRGCYC 2 cut(s) 99, 105
ErhI CCWWGG 4 cut(s) 138, 148, 782, 854
FaeI CATG 3 cut(s) 34, 674, 826
FaqI GGGAC 2 cut(s) 310, 639
FatI CATG 3 cut(s) 30, 670, 822
Fnu4HI GCNGC 3 cut(s) 127, 135, 291
FokI GGATG 5 cut(s) 354, 551, 775, 788, 802
FriOI GRGCYC 2 cut(s) 99, 105
Fsp4HI GCNGC 3 cut(s) 127, 135, 291
FspBI CTAG 1 cut(s) 596
FspI TGCGCA 1 cut(s) 576
GlaI GCGC 2 cut(s) 503, 576
GluI GCNGC 3 cut(s) 127, 135, 291
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 1 cut(s) 629
HaeII RGCGCY 1 cut(s) 505
HaeIII GGCC 4 cut(s) 114, 143, 153, 346
HapII CCGG 1 cut(s) 196
HhaI GCGC 2 cut(s) 504, 577
Hin1II CATG 3 cut(s) 34, 674, 826
Hin6I GCGC 2 cut(s) 502, 575
HinP1I GCGC 2 cut(s) 502, 575
HincII GTYRAC 2 cut(s) 415, 565
HindII GTYRAC 2 cut(s) 415, 565
HinfI GANTC 4 cut(s) 71, 89, 314, 819
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 1 cut(s) 196
HphI GGTGA 3 cut(s) 440, 541, 767
Hpy166II GTNNAC 2 cut(s) 415, 565
Hpy188I TCNGA 6 cut(s) 67, 94, 220, 356, 421, 835
Hpy188III TCNNGA 3 cut(s) 470, 665, 823
Hpy8I GTNNAC 2 cut(s) 415, 565
Hpy99I CGWCG 5 cut(s) 232, 253, 256, 259, 703
HpyAV CCTTC 4 cut(s) 15, 445, 549, 746
HpyCH4III ACNGT 4 cut(s) 329, 581, 630, 699
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 4 cut(s) 435, 731, 875, 888
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 140
HpyF3I CTNAG 8 cut(s) 93, 116, 186, 300, 418, 489, 797, 815
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 3 cut(s) 34, 674, 826
HspAI GCGC 2 cut(s) 502, 575
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 1 cut(s) 716
LmnI GCTCC 3 cut(s) 162, 667, 856
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 2 cut(s) 753, 875
MaeI CTAG 1 cut(s) 596
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 3 cut(s) 22, 77, 755
MalI GATC 1 cut(s) 718
MboI GATC 1 cut(s) 716
MboII GAAGA 9 cut(s) 54, 237, 252, 255, 258, 387, 624, 755, 820
MfeI CAATTG 2 cut(s) 156, 549
MhlI GDGCHC 2 cut(s) 99, 105
MluCI AATT 4 cut(s) 156, 370, 549, 644
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 370
MseI TTAA 2 cut(s) 282, 414
MslI CAYNNNNRTG 1 cut(s) 29
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 1 cut(s) 623
MunI CAATTG 2 cut(s) 156, 549
Mva1269I GAATGC 2 cut(s) 437, 877
MvaI CCWGG 1 cut(s) 623
MwoI GCNNNNNNNGC 2 cut(s) 126, 140
NdeII GATC 1 cut(s) 716
NlaIII CATG 3 cut(s) 34, 674, 826
NlaIV GGNNCC 1 cut(s) 627
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 2 cut(s) 22, 755
NsbI TGCGCA 1 cut(s) 576
NspI RCATGY 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 1 cut(s) 822
PciI ACATGT 1 cut(s) 30
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 254
PctI GAATGC 2 cut(s) 437, 877
PdmI GAANNNNTTC 1 cut(s) 370
PfeI GAWTC 3 cut(s) 71, 314, 819
PkrI GCNGC 3 cut(s) 128, 136, 292
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Ppu21I YACGTR 1 cut(s) 653
PscI ACATGT 1 cut(s) 30
Psp124BI GAGCTC 2 cut(s) 99, 105
Psp6I CCWGG 1 cut(s) 621
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 3 cut(s) 142, 152, 626
PspXI VCTCGAGB 1 cut(s) 98
PstNI CAGNNNCTG 2 cut(s) 14, 404
RseI CAYNNNNRTG 1 cut(s) 29
SacI GAGCTC 2 cut(s) 99, 105
SaqAI TTAA 2 cut(s) 282, 414
SatI GCNGC 3 cut(s) 127, 135, 291
Sau3AI GATC 1 cut(s) 716
Sau96I GGNCC 3 cut(s) 142, 152, 626
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 623
SduI GDGCHC 2 cut(s) 99, 105
SfaNI GCATC 2 cut(s) 753, 875
SfcI CTRYAG 1 cut(s) 860
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 156, 370, 549, 644
SsiI CCGC 5 cut(s) 126, 129, 233, 290, 304
SspMI CTAG 1 cut(s) 596
SstI GAGCTC 2 cut(s) 99, 105
StyD4I CCNGG 1 cut(s) 621
StyI CCWWGG 4 cut(s) 138, 148, 782, 854
TaaI ACNGT 4 cut(s) 329, 581, 630, 699
TaiI ACGT 1 cut(s) 655
TaqI TCGA 3 cut(s) 35, 99, 701
TasI AATT 4 cut(s) 156, 370, 549, 644
TauI GCSGC 2 cut(s) 129, 293
TfiI GAWTC 3 cut(s) 71, 314, 819
Tru1I TTAA 2 cut(s) 282, 414
Tru9I TTAA 2 cut(s) 282, 414
TscAI CASTG 3 cut(s) 31, 283, 409
TseFI GTSAC 2 cut(s) 22, 755
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 2 cut(s) 22, 755
TspDTI ATGAA 4 cut(s) 371, 383, 603, 756
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 3 cut(s) 31, 283, 409
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 370
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.