MD05G1188800.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009009
Physical Location & Seq
Reverse (-)
31653742 .. 31656640
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1188800.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGAGAGTGGAGGTTCTGCAAGTGGGCCGTCGACTAAGTCAACGCTGTCCAGGGAGGAGAATTTTGCCATGTCTTCTGAGGACTCTTCTACCCCTGAGGAGTCTGGGCTCGAGCTCTGTCTTGGGCTGAGCCTTGGTGGTGGTGGTGGTGGTGGTGGTAAGGGTCAGCAAGGGCAAAGGGGTCAAGTTGCTAGGATCTTGACTGCTAAGGATTTTCCTTCTGCGGGGTTTTCTTCTTCGTCGGCTTCCAAGTCGTCGTCTTCTACTTCTTCTTCGTTGAGCAGTGCTAATGTTGCGGCTGGAACCAAGAGAAGTGCTGATTCTGTGGCTGCTGCTAATGGTGGCAGTCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAACAGTTTGGTTCACCAAGCGAAATCTTCATCTGCCGAAGGATCTAACTCAGTAAATGAGAGAAGCGAGTGCAAGACTGGTGCGGGGAAGGTTAACAATGGAGGTCACAAGACCAACGGAAATGCTAAGGAGATAGGGCAGCAGAGTGGTTCCCTGTTTGTGAAGGTGAATATGGACGGGATTCCTATTGGGCGAAAGGTTAATTTAAGTGCACATAGTTCGTATGAAGCCCTAGCACAAAAATTGGAGGATATGTTCCATGGGCCCTCGACACATGGTGGTCCGGAGATGGAAGGAGCAACTAGACCCTCAAAATTGCTGGATGGATCATTTGAGTTTGCGCTCACTTACGAAGATAAAGATGGAGACTGGATGCTTGTGGGAGATGTTCCTTGGGAGATGTTTCTTGGTACTGTGAAGAGGTTGAGGATTATGAAGACATCTGAGGATAACGGACTTGCTCCGCTGTTACGGGAAAAGAATGTGAGGCAAAGATGTAAGCCAATCTAG

Protein Analysis

299

Amino Acids

31.01

Weight (kDa)

8.36

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 38 - 284 2.1e-60 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 670
AciI CCGC 4 cut(s) 224, 296, 470, 851
AclWI GGATC 3 cut(s) 203, 436, 721
AcsI RAATTY 1 cut(s) 61
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 1 cut(s) 224
AjnI CCWGG 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 626, 658
AluBI AGCT 2 cut(s) 115, 377
AluI AGCT 2 cut(s) 115, 377
Alw21I GWGCWC 2 cut(s) 117, 601
Alw26I GTCTC 1 cut(s) 747
Alw44I GTGCAC 1 cut(s) 597
AlwI GGATC 3 cut(s) 203, 436, 721
Ama87I CYCGRG 1 cut(s) 110
Aor13HI TCCGGA 1 cut(s) 670
AoxI GGCC 3 cut(s) 26, 362, 650
ApaI GGGCCC 1 cut(s) 654
ApaLI GTGCAC 1 cut(s) 597
ApeKI GCWGC 3 cut(s) 329, 332, 526
ApoI RAATTY 1 cut(s) 61
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 4 cut(s) 26, 650, 651, 668
AsuHPI GGTGA 2 cut(s) 392, 565
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 668
AxyI CCTNAGG 1 cut(s) 96
BaeGI GKGCMC 2 cut(s) 601, 654
BanII GRGCYC 3 cut(s) 111, 117, 654
BbsI GAAGAC 3 cut(s) 66, 252, 830
Bbv12I GWGCWC 2 cut(s) 117, 601
BbvI GCAGC 3 cut(s) 316, 319, 538
BccI CCATC 4 cut(s) 354, 670, 704, 743
BceAI ACGGC 1 cut(s) 13
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 747
BfaI CTAG 4 cut(s) 192, 620, 690, 895
BisI GCNGC 4 cut(s) 297, 330, 333, 527
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 4 cut(s) 298, 331, 334, 528
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 1 cut(s) 668
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 4 cut(s) 26, 650, 651, 668
BmiI GGNNCC 3 cut(s) 304, 538, 652
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 750
BpiI GAAGAC 3 cut(s) 66, 252, 830
Bpu10I CCTNAGC 2 cut(s) 207, 513
Bpu1102I GCTNAGC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 51, 133, 646, 779
BsaWI WCCGGW 1 cut(s) 670
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 469, 761
Bse21I CCTNAGG 1 cut(s) 96
BseAI TCCGGA 1 cut(s) 670
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 4 cut(s) 51, 133, 646, 779
BseGI GGATG 3 cut(s) 365, 715, 765
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMII CTCAG 5 cut(s) 69, 87, 119, 450, 822
BseNI ACTGG 2 cut(s) 469, 761
BseRI GAGGAG 2 cut(s) 71, 113
BseSI GKGCMC 2 cut(s) 601, 654
BseXI GCAGC 3 cut(s) 316, 319, 538
BshFI GGCC 3 cut(s) 28, 364, 652
BsiHKAI GWGCWC 2 cut(s) 117, 601
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 747
BsnI GGCC 3 cut(s) 28, 364, 652
BsoBI CYCGRG 1 cut(s) 110
Bsp120I GGGCCC 1 cut(s) 650
Bsp1286I GDGCHC 4 cut(s) 111, 117, 601, 654
Bsp13I TCCGGA 1 cut(s) 670
Bsp143I GATC 3 cut(s) 195, 428, 713
Bsp1720I GCTNAGC 1 cut(s) 128
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 4 cut(s) 224, 296, 470, 851
BspANI GGCC 3 cut(s) 28, 364, 652
BspCNI CTCAG 5 cut(s) 70, 88, 120, 449, 823
BspEI TCCGGA 1 cut(s) 670
BspLI GGNNCC 3 cut(s) 304, 538, 652
BspPI GGATC 3 cut(s) 203, 436, 721
BsrI ACTGG 2 cut(s) 469, 761
BssECI CCNNGG 4 cut(s) 51, 133, 646, 779
BssMI GATC 3 cut(s) 195, 428, 713
BssT1I CCWWGG 3 cut(s) 133, 646, 779
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 2 cut(s) 392, 802
Bst6I CTCTTC 2 cut(s) 91, 800
BstDEI CTNAG 8 cut(s) 36, 78, 96, 128, 207, 436, 513, 831
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 3 cut(s) 365, 715, 765
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 3 cut(s) 198, 431, 716
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 3 cut(s) 195, 428, 713
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstSLI GKGCMC 2 cut(s) 601, 654
BstV1I GCAGC 3 cut(s) 316, 319, 538
BstV2I GAAGAC 3 cut(s) 66, 252, 830
BstX2I RGATCY 2 cut(s) 195, 428
BstYI RGATCY 2 cut(s) 195, 428
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 3 cut(s) 28, 364, 652
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 3 cut(s) 365, 715, 765
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 4 cut(s) 26, 650, 651, 668
Csp6I GTAC 1 cut(s) 798
CviAII CATG 3 cut(s) 70, 647, 662
CviQI GTAC 1 cut(s) 798
DdeI CTNAG 8 cut(s) 36, 78, 96, 128, 207, 436, 513, 831
DpnI GATC 3 cut(s) 197, 430, 715
DpnII GATC 3 cut(s) 195, 428, 713
DraIII CACNNNGTG 1 cut(s) 665
Eam1104I CTCTTC 2 cut(s) 91, 800
EarI CTCTTC 2 cut(s) 91, 800
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 133, 646, 779
Eco24I GRGCYC 3 cut(s) 111, 117, 654
Eco47I GGWCC 1 cut(s) 668
Eco53kI GAGCTC 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 3 cut(s) 133, 646, 779
EcoT38I GRGCYC 3 cut(s) 111, 117, 654
ErhI CCWWGG 3 cut(s) 133, 646, 779
FaeI CATG 3 cut(s) 73, 650, 665
FaiI YATR 9 cut(s) 71, 381, 560, 603, 612, 641, 648, 663, 821
FatI CATG 3 cut(s) 69, 646, 661
FauI CCCGC 2 cut(s) 217, 463
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 297, 330, 333, 527
FokI GGATG 3 cut(s) 372, 722, 772
FriOI GRGCYC 3 cut(s) 111, 117, 654
Fsp4HI GCNGC 4 cut(s) 297, 330, 333, 527
FspBI CTAG 4 cut(s) 192, 620, 690, 895
GlaI GCGC 1 cut(s) 729
GluI GCNGC 4 cut(s) 297, 330, 333, 527
HaeIII GGCC 3 cut(s) 28, 364, 652
HapII CCGG 1 cut(s) 671
HhaI GCGC 1 cut(s) 730
Hin1II CATG 3 cut(s) 73, 650, 665
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 3 cut(s) 33, 42, 481
HindII GTYRAC 3 cut(s) 33, 42, 481
HinfI GANTC 4 cut(s) 83, 101, 320, 568
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 1 cut(s) 671
HphI GGTGA 2 cut(s) 392, 565
Hpy166II GTNNAC 5 cut(s) 33, 42, 400, 481, 599
Hpy188I TCNGA 3 cut(s) 79, 374, 832
Hpy188III TCNNGA 2 cut(s) 199, 671
Hpy8I GTNNAC 5 cut(s) 33, 42, 400, 481, 599
Hpy99I CGWCG 3 cut(s) 34, 244, 259
HpyAV CCTTC 5 cut(s) 228, 419, 469, 544, 674
HpyCH4III ACNGT 2 cut(s) 392, 802
HpyCH4V TGCA 3 cut(s) 20, 459, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 8 cut(s) 36, 78, 96, 128, 207, 436, 513, 831
Hsp92II CATG 3 cut(s) 73, 650, 665
HspAI GCGC 1 cut(s) 728
Kpn2I TCCGGA 1 cut(s) 670
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 3 cut(s) 195, 428, 713
LmnI GCTCC 2 cut(s) 683, 853
Lsp1109I GCAGC 3 cut(s) 316, 319, 538
LweI GCATC 1 cut(s) 750
MaeI CTAG 4 cut(s) 192, 620, 690, 895
MaeIII GTNAC 2 cut(s) 491, 855
MalI GATC 3 cut(s) 197, 430, 715
MboI GATC 3 cut(s) 195, 428, 713
MflI RGATCY 2 cut(s) 195, 428
MhlI GDGCHC 4 cut(s) 111, 117, 601, 654
MluCI AATT 4 cut(s) 61, 589, 629, 701
MlyI GAGTC 2 cut(s) 77, 110
MroI TCCGGA 1 cut(s) 670
MseI TTAA 3 cut(s) 480, 588, 593
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 1 cut(s) 671
MspR9I CCNGG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 293
NcoI CCATGG 1 cut(s) 646
NdeII GATC 3 cut(s) 195, 428, 713
NlaIII CATG 3 cut(s) 73, 650, 665
NlaIV GGNNCC 3 cut(s) 304, 538, 652
NmuCI GTSAC 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 110
PfeI GAWTC 2 cut(s) 320, 568
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 4 cut(s) 298, 331, 334, 528
PleI GAGTC 2 cut(s) 77, 109
PpsI GAGTC 2 cut(s) 77, 109
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 3 cut(s) 304, 538, 652
PspOMI GGGCCC 1 cut(s) 650
PspPI GGNCC 4 cut(s) 26, 650, 651, 668
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 2 cut(s) 195, 428
PsyI GACNNNGTC 1 cut(s) 37
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 31
SaqAI TTAA 3 cut(s) 480, 588, 593
SatI GCNGC 4 cut(s) 297, 330, 333, 527
Sau3AI GATC 3 cut(s) 195, 428, 713
Sau96I GGNCC 4 cut(s) 26, 650, 651, 668
SchI GAGTC 2 cut(s) 77, 110
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 4 cut(s) 111, 117, 601, 654
SetI ASST 9 cut(s) 16, 117, 354, 379, 480, 493, 555, 588, 812
SfaNI GCATC 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 110
SinI GGWCC 1 cut(s) 668
SlaI CTCGAG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 61, 589, 629, 701
SsiI CCGC 4 cut(s) 224, 296, 470, 851
SspMI CTAG 4 cut(s) 192, 620, 690, 895
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 3 cut(s) 133, 646, 779
TaaI ACNGT 2 cut(s) 392, 802
TaqI TCGA 3 cut(s) 32, 111, 656
TasI AATT 4 cut(s) 61, 589, 629, 701
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 2 cut(s) 320, 568
Tru1I TTAA 3 cut(s) 480, 588, 593
Tru9I TTAA 3 cut(s) 480, 588, 593
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 3 cut(s) 329, 332, 526
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 4 cut(s) 401, 405, 627, 836
TspGWI ACGGA 2 cut(s) 519, 855
TspRI CASTG 1 cut(s) 289
Tth111I GACNNNGTC 1 cut(s) 37
VneI GTGCAC 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 4 cut(s) 192, 620, 690, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.