Rw6G025320

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009009
Physical Location & Seq
Reverse (-)
48643647 .. 48646543
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G025320.1

Sequence Viewer

Length: 924 bp
ATGCAGGGTTTTTCCGGAGGTTTAGGAACTGGGTCTGTGACTAACATGTCGACCGTCTCCAAAGATGAGAACTTTGCTATGTCTTCTGAGGATTCTTGTAGCCCAGATGAGTCTGACCTCGAGCTCTGTCTTGGGCTGAGTCTCGGCGGTGGTGGGAAGGCTGCCCAGCAAGGCCCAGTTGGGAATTATGCTAGGATTCTGACGGCCAAGGATTTCCGTTATGTGGGTTCTAACTCTAAGTCGTCGGCGGCGGCTGCTTCTTCATCGTCGTCGTCGTCTTCTTCGTTGAGCAGTGCTAATGTTACGGCTGGGACTAAGAGGAGTGCTGATTCTGTGGCGGCGGCTAATGCTGCCAATCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAATTCCTTGGTTCATCAAGCAAAATCTTCAGCCACTGAAGTATTTAACTCGGAGAAAGGTGAATGCCAGATTACTTCAGGGAAGGGTAACATTGGTGGTCAAGAAAACAATGCAAATGCTAAGGAGGGAGGACGCCTCACTAGTTCCCTGTTTGTGAAGGTGAATATGGATGGAAGTCCAATCGGAAGGAAGGTTAACCTAACTGCGCATAGCAGCTATGAAGCCCTAGCACAAACGTTGGAGGATATGTTCCAGGGACCCTCTGCAAATGCGAAATTGATACGTTCTGGTGGTCAGGAGCATGATATAATGGTGGGAGAAACTACACCCTCAAAGTTGCTAGATGGATCATTTGATTTTGCACTCACTTATGAAGACAAAGAGGGCGACTGGATGCTTGTTGGGGATGTTCCTTGGGGGATGTTTCTTTGTTCTGTGAAGAGGCTAAGAATCATGAGGACATCTGAGGCTAATGGACTTGCTCCGAGGCTGCAGGAAAGGAATGCAAGGCAGAGATGCGCGAGCTGA

Protein Analysis

307

Amino Acids

32.19

Weight (kDa)

6.34

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 40 - 295 2.1e-64 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
Acc16I TGCGCA 1 cut(s) 603
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 917
AccIII TCCGGA 1 cut(s) 14
AciI CCGC 5 cut(s) 147, 248, 251, 338, 341
AclI AACGTT 1 cut(s) 632
AclWI GGATC 1 cut(s) 751
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 3 cut(s) 408, 453, 456
AcyI GRCGYC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 45
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 648
AloI GAACNNNNNNTCC 2 cut(s) 629, 661
AluBI AGCT 4 cut(s) 124, 386, 612, 921
AluI AGCT 4 cut(s) 124, 386, 612, 921
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 61, 146
AlwI GGATC 1 cut(s) 751
AlwNI CAGNNNCTG 1 cut(s) 431
Ama87I CYCGRG 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 14
AoxI GGCC 3 cut(s) 172, 204, 371
ApeKI GCWGC 5 cut(s) 161, 254, 350, 609, 886
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 397
AspLEI GCGC 2 cut(s) 604, 917
AspS9I GGNCC 2 cut(s) 173, 653
AsuHPI GGTGA 2 cut(s) 467, 568
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 3 cut(s) 75, 270, 777
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 5 cut(s) 148, 241, 337, 621, 873
BccI CCATC 3 cut(s) 363, 560, 734
BceAI ACGGC 2 cut(s) 219, 321
BciT130I CCWGG 1 cut(s) 650
BcoDI GTCTC 2 cut(s) 61, 146
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 4 cut(s) 192, 537, 623, 737
BfmI CTRYAG 1 cut(s) 887
BisI GCNGC 9 cut(s) 162, 249, 252, 255, 339, 342, 351, 610, 887
BlsI GCNGC 9 cut(s) 163, 250, 253, 256, 340, 343, 352, 611, 888
Bme1390I CCNGG 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 653
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 173, 653
BmiI GGNNCC 2 cut(s) 654, 655
BmrFI CCNGG 1 cut(s) 650
BmrI ACTGGG 2 cut(s) 39, 170
BmsI GCATC 2 cut(s) 780, 902
BmuI ACTGGG 2 cut(s) 39, 170
BpiI GAAGAC 3 cut(s) 75, 270, 777
BplI GAGNNNNNCTC 2 cut(s) 516, 548
Bpu10I CCTNAGC 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 529
BsaJI CCNNGG 5 cut(s) 207, 402, 649, 809, 881
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 34, 176, 791
BseAI TCCGGA 1 cut(s) 14
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 5 cut(s) 207, 402, 649, 809, 881
BseGI GGATG 5 cut(s) 374, 571, 795, 808, 822
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 3 cut(s) 78, 128, 852
BseNI ACTGG 3 cut(s) 34, 176, 791
BseRI GAGGAG 1 cut(s) 334
BseXI GCAGC 5 cut(s) 148, 241, 337, 621, 873
BseYI CCCAGC 2 cut(s) 165, 308
Bsh1236I CGCG 1 cut(s) 917
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 174, 206, 373
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 15
BslFI GGGAC 2 cut(s) 325, 666
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 2 cut(s) 61, 146
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 325, 666
BsmI GAATGC 2 cut(s) 464, 904
BsnI GGCC 3 cut(s) 174, 206, 373
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 126
Bsp13I TCCGGA 1 cut(s) 14
Bsp143I GATC 1 cut(s) 743
BspACI CCGC 5 cut(s) 147, 248, 251, 338, 341
BspANI GGCC 3 cut(s) 174, 206, 373
BspCNI CTCAG 3 cut(s) 79, 129, 853
BspEI TCCGGA 1 cut(s) 14
BspFNI CGCG 1 cut(s) 917
BspHI TCATGA 1 cut(s) 849
BspLI GGNNCC 2 cut(s) 654, 655
BspMAI CTGCAG 1 cut(s) 891
BspPI GGATC 1 cut(s) 751
BsrI ACTGG 3 cut(s) 34, 176, 791
BssECI CCNNGG 5 cut(s) 207, 402, 649, 809, 881
BssMI GATC 1 cut(s) 743
BssNI GRCGYC 1 cut(s) 529
BssT1I CCWWGG 3 cut(s) 207, 402, 809
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 830
BstACI GRCGYC 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 919
BstDEI CTNAG 7 cut(s) 87, 137, 237, 315, 516, 842, 861
BstF5I GGATG 5 cut(s) 374, 571, 795, 808, 822
BstFNI CGCG 1 cut(s) 917
BstHHI GCGC 2 cut(s) 604, 917
BstKTI GATC 1 cut(s) 746
BstMAI GTCTC 2 cut(s) 61, 146
BstMBI GATC 1 cut(s) 743
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 254, 347, 350
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 648
BstSFI CTRYAG 1 cut(s) 887
BstUI CGCG 1 cut(s) 917
BstV1I GCAGC 5 cut(s) 148, 241, 337, 621, 873
BstV2I GAAGAC 3 cut(s) 75, 270, 777
BsuRI GGCC 3 cut(s) 174, 206, 373
BtsCI GGATG 5 cut(s) 374, 571, 795, 808, 822
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 298, 429
Cac8I GCNNGC 1 cut(s) 919
CaiI CAGNNNCTG 1 cut(s) 431
CciI TCATGA 1 cut(s) 849
CfoI GCGC 2 cut(s) 604, 917
Cfr13I GGNCC 2 cut(s) 173, 653
CseI GACGC 1 cut(s) 537
CviAII CATG 3 cut(s) 46, 698, 850
DdeI CTNAG 7 cut(s) 87, 137, 237, 315, 516, 842, 861
DpnI GATC 1 cut(s) 745
DpnII GATC 1 cut(s) 743
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 830
EarI CTCTTC 1 cut(s) 830
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 207, 402, 809
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 653
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 3 cut(s) 408, 453, 456
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 3 cut(s) 207, 402, 809
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 207, 402, 809
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 3 cut(s) 49, 701, 853
FaqI GGGAC 2 cut(s) 325, 666
FatI CATG 3 cut(s) 45, 697, 849
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 9 cut(s) 162, 249, 252, 255, 339, 342, 351, 610, 887
FokI GGATG 5 cut(s) 381, 578, 802, 815, 829
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 9 cut(s) 162, 249, 252, 255, 339, 342, 351, 610, 887
FspBI CTAG 4 cut(s) 192, 537, 623, 737
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 2 cut(s) 603, 916
GluI GCNGC 9 cut(s) 162, 249, 252, 255, 339, 342, 351, 610, 887
GsaI CCCAGC 2 cut(s) 169, 312
HaeIII GGCC 3 cut(s) 174, 206, 373
HapII CCGG 1 cut(s) 15
HgaI GACGC 1 cut(s) 537
HhaI GCGC 2 cut(s) 604, 917
Hin1I GRCGYC 1 cut(s) 529
Hin1II CATG 3 cut(s) 49, 701, 853
Hin6I GCGC 2 cut(s) 602, 915
HinP1I GCGC 2 cut(s) 602, 915
HincII GTYRAC 2 cut(s) 51, 592
HindII GTYRAC 2 cut(s) 51, 592
HinfI GANTC 6 cut(s) 92, 110, 139, 196, 329, 846
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 1 cut(s) 15
HphI GGTGA 2 cut(s) 467, 568
Hpy166II GTNNAC 2 cut(s) 51, 592
Hpy188I TCNGA 8 cut(s) 88, 115, 201, 383, 448, 581, 862, 882
Hpy188III TCNNGA 4 cut(s) 15, 497, 692, 850
Hpy8I GTNNAC 2 cut(s) 51, 592
Hpy99I CGWCG 4 cut(s) 247, 271, 274, 277
HpyAV CCTTC 5 cut(s) 151, 472, 547, 576, 580
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 2 cut(s) 632, 679
HpyCH4V TGCA 6 cut(s) 4, 509, 662, 758, 889, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 347, 350
HpyF3I CTNAG 7 cut(s) 87, 137, 237, 315, 516, 842, 861
HpySE526I ACGT 2 cut(s) 632, 679
Hsp92I GRCGYC 1 cut(s) 529
Hsp92II CATG 3 cut(s) 49, 701, 853
HspAI GCGC 2 cut(s) 602, 915
KflI GGGWCCC 1 cut(s) 653
Kpn2I TCCGGA 1 cut(s) 14
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 1 cut(s) 743
LmnI GCTCC 2 cut(s) 694, 883
Lsp1109I GCAGC 5 cut(s) 148, 241, 337, 621, 873
LweI GCATC 2 cut(s) 780, 902
MaeI CTAG 4 cut(s) 192, 537, 623, 737
MaeII ACGT 2 cut(s) 632, 679
MaeIII GTNAC 3 cut(s) 37, 301, 482
MalI GATC 1 cut(s) 745
MboI GATC 1 cut(s) 743
MboII GAAGA 7 cut(s) 75, 252, 270, 273, 414, 782, 847
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 3 cut(s) 184, 397, 671
MlyI GAGTC 2 cut(s) 119, 148
MmeI TCCRAC 1 cut(s) 615
MroI TCCGGA 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 397
MseI TTAA 2 cut(s) 441, 591
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 1 cut(s) 650
Mva1269I GAATGC 2 cut(s) 464, 904
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 1 cut(s) 917
MwoI GCNNNNNNNGC 3 cut(s) 254, 347, 350
NdeII GATC 1 cut(s) 743
NlaIII CATG 3 cut(s) 49, 701, 853
NlaIV GGNNCC 2 cut(s) 654, 655
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 37
NsbI TGCGCA 1 cut(s) 603
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 1 cut(s) 849
PciI ACATGT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 2 cut(s) 272, 281
PctI GAATGC 2 cut(s) 464, 904
PdmI GAANNNNTTC 1 cut(s) 397
PfeI GAWTC 4 cut(s) 92, 196, 329, 846
PkrI GCNGC 9 cut(s) 163, 250, 253, 256, 340, 343, 352, 611, 888
PleI GAGTC 2 cut(s) 118, 147
PpsI GAGTC 2 cut(s) 118, 147
PpuMI RGGWCCY 1 cut(s) 653
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 126
Psp1406I AACGTT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 648
PspFI CCCAGC 2 cut(s) 165, 308
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 2 cut(s) 654, 655
PspPI GGNCC 2 cut(s) 173, 653
PspPPI RGGWCCY 1 cut(s) 653
PspXI VCTCGAGB 1 cut(s) 119
PstI CTGCAG 1 cut(s) 891
PstNI CAGNNNCTG 1 cut(s) 431
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 441, 591
SatI GCNGC 9 cut(s) 162, 249, 252, 255, 339, 342, 351, 610, 887
Sau3AI GATC 1 cut(s) 743
Sau96I GGNCC 2 cut(s) 173, 653
SchI GAGTC 2 cut(s) 119, 148
ScrFI CCNGG 1 cut(s) 650
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 2 cut(s) 780, 902
SfcI CTRYAG 1 cut(s) 887
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 536
Sse9I AATT 3 cut(s) 184, 397, 671
SsiI CCGC 5 cut(s) 147, 248, 251, 338, 341
SspMI CTAG 4 cut(s) 192, 537, 623, 737
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 648
StyI CCWWGG 3 cut(s) 207, 402, 809
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 2 cut(s) 635, 682
TaqI TCGA 2 cut(s) 50, 120
TasI AATT 3 cut(s) 184, 397, 671
TauI GCSGC 4 cut(s) 251, 254, 341, 344
TfiI GAWTC 4 cut(s) 92, 196, 329, 846
Tru1I TTAA 2 cut(s) 441, 591
Tru9I TTAA 2 cut(s) 441, 591
TscAI CASTG 2 cut(s) 298, 436
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 5 cut(s) 161, 254, 350, 609, 886
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 5 cut(s) 252, 398, 410, 630, 783
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 298, 436
VpaK11BI GGWCC 1 cut(s) 653
XapI RAATTY 1 cut(s) 397
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 397
XspI CTAG 4 cut(s) 192, 537, 623, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.