Rh6CG297800

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009009
Physical Location & Seq
Reverse (-)
47882089 .. 47885376
3288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG297800.1

Sequence Viewer

Length: 882 bp
ATGCAGGGTTTTTCTGGAGGTTTAGGAACTGGGTCTGTGACTAACATGTCGACCGTGTCCAAAGATGAGAACTTTGCTATGTCTTCTGAGGATTCTTGTAGCCCAGATGAGTCTGACCTCGAGCTCTGTCTTGGGCTGAGTCTCGGCGGTGGCGGGAAGGCCGCCCAGCAAGGCCCAGTTGGGAATTATGCTAGGATTCTGACGGCCAAGGATTTCCGTTATGTGGGTTCTAACTCTAAGTCGTCGGCGGCCGCTGCTTCTTCTTCGTCGTCGTCTTCTTCGTTGAGCAGTGCTAATGTTACGGCTGGGACTAAGAGGAGTGCTGATTCTGTGGCGGCGGCTAATGGTGCCAATCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAATTCCTTGGTTCATCAAGCAAAATCTTCAGCCACTGAAGTATTTAACTCGGAGAAAGGTGAATGCCAGATTACTTCAGGGAAGGGTAACATTGGTGGTCAAGAAAACAATGCAAATGCTAAGGAGGGAGGACGCCTCACTAGCTCCCTGTTTGTGAAGGTGAATATGGATGGAAGTGCAATCGGAAGGAAGGTTAACCTAACTGCGCATAGCAGCTATGAAGCCCTAGCACAAACTTTGGAGGATATGTTCCAGGGACCCTCTGCAAATGCGAAATTGATACGTTCTGGTGGTCAGGAGCATGATATAATGGTGGGAGAAACTACACCCTCAAAATTGCTAGATGGATCATTTGATTTTGCACTCACTTATGAAGACAAAGAAGGCGACTGGATGCTTGTTGGGGATGTTCCTTGGGGGATGTTTCTTTGTTCTGTGAAGAGGCTAAGAATCATGAGGACATCTGAGGCTAATGGACTTGGTATGTAG

Protein Analysis

293

Amino Acids

30.51

Weight (kDa)

5.5

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 40 - 292 5.7e-61 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
Acc16I TGCGCA 1 cut(s) 600
AccB1I GGYRCC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 50
AciI CCGC 7 cut(s) 147, 153, 162, 248, 252, 335, 338
AclWI GGATC 1 cut(s) 748
AcoI YGGCCR 2 cut(s) 204, 249
AcsI RAATTY 1 cut(s) 394
AcuI CTGAAG 3 cut(s) 405, 450, 453
AcyI GRCGYC 1 cut(s) 526
AfiI CCNNNNNNNGG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 45
AjnI CCWGG 1 cut(s) 645
AloI GAACNNNNNNTCC 2 cut(s) 626, 658
AluBI AGCT 4 cut(s) 124, 383, 537, 609
AluI AGCT 4 cut(s) 124, 383, 537, 609
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 748
AlwNI CAGNNNCTG 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 5 cut(s) 159, 172, 204, 249, 368
ApeKI GCWGC 2 cut(s) 254, 606
ApoI RAATTY 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 394
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 2 cut(s) 173, 650
AsuHPI GGTGA 2 cut(s) 464, 565
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 3 cut(s) 75, 267, 774
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 241, 618
BccI CCATC 3 cut(s) 360, 557, 731
BceAI ACGGC 2 cut(s) 219, 318
BciT130I CCWGG 1 cut(s) 647
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 4 cut(s) 192, 534, 620, 734
BisI GCNGC 7 cut(s) 162, 249, 252, 255, 336, 339, 607
BlsI GCNGC 7 cut(s) 163, 250, 253, 256, 337, 340, 608
Bme1390I CCNGG 1 cut(s) 647
Bme18I GGWCC 1 cut(s) 650
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 173, 650
BmiI GGNNCC 3 cut(s) 349, 651, 652
BmrFI CCNGG 1 cut(s) 647
BmrI ACTGGG 2 cut(s) 39, 170
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 2 cut(s) 39, 170
BpiI GAAGAC 3 cut(s) 75, 267, 774
BplI GAGNNNNNCTC 2 cut(s) 513, 545
BpmI CTGGAG 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 526
BsaJI CCNNGG 4 cut(s) 207, 399, 646, 806
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 34, 176, 788
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 207, 399, 646, 806
BseGI GGATG 5 cut(s) 371, 568, 792, 805, 819
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 3 cut(s) 78, 128, 849
BseNI ACTGG 3 cut(s) 34, 176, 788
BseRI GAGGAG 1 cut(s) 331
BseX3I CGGCCG 1 cut(s) 249
BseXI GCAGC 2 cut(s) 241, 618
BseYI CCCAGC 2 cut(s) 165, 305
Bsh1285I CGRYCG 2 cut(s) 54, 252
BshFI GGCC 5 cut(s) 161, 174, 206, 251, 370
BshNI GGYRCC 1 cut(s) 347
BsiEI CGRYCG 2 cut(s) 54, 252
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 119
BslFI GGGAC 2 cut(s) 322, 663
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 322, 663
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 5 cut(s) 161, 174, 206, 251, 370
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 1 cut(s) 740
BspACI CCGC 7 cut(s) 147, 153, 162, 248, 252, 335, 338
BspANI GGCC 5 cut(s) 161, 174, 206, 251, 370
BspCNI CTCAG 3 cut(s) 79, 129, 850
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 349, 651, 652
BspPI GGATC 1 cut(s) 748
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 3 cut(s) 34, 176, 788
BssECI CCNNGG 4 cut(s) 207, 399, 646, 806
BssMI GATC 1 cut(s) 740
BssNI GRCGYC 1 cut(s) 526
BssT1I CCWWGG 3 cut(s) 207, 399, 806
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 1 cut(s) 526
BstDEI CTNAG 7 cut(s) 87, 137, 237, 312, 513, 839, 858
BstF5I GGATG 5 cut(s) 371, 568, 792, 805, 819
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 1 cut(s) 743
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 1 cut(s) 740
BstMCI CGRYCG 2 cut(s) 54, 252
BstMWI GCNNNNNNNGC 3 cut(s) 254, 347, 534
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 645
BstV1I GCAGC 2 cut(s) 241, 618
BstV2I GAAGAC 3 cut(s) 75, 267, 774
BstZI CGGCCG 1 cut(s) 249
BsuRI GGCC 5 cut(s) 161, 174, 206, 251, 370
BtsCI GGATG 5 cut(s) 371, 568, 792, 805, 819
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 295, 426
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 1 cut(s) 846
CciNI GCGGCCGC 1 cut(s) 249
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 2 cut(s) 173, 650
CseI GACGC 1 cut(s) 534
CviAII CATG 3 cut(s) 46, 695, 847
DdeI CTNAG 7 cut(s) 87, 137, 237, 312, 513, 839, 858
DpnI GATC 1 cut(s) 742
DpnII GATC 1 cut(s) 740
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 204, 249
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
Ecl136II GAGCTC 1 cut(s) 124
EclXI CGGCCG 1 cut(s) 249
Eco130I CCWWGG 3 cut(s) 207, 399, 806
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 650
Eco52I CGGCCG 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 3 cut(s) 405, 450, 453
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 645
EcoT14I CCWWGG 3 cut(s) 207, 399, 806
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 207, 399, 806
FaeI CATG 3 cut(s) 49, 698, 850
FaqI GGGAC 2 cut(s) 322, 663
FatI CATG 3 cut(s) 45, 694, 846
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 7 cut(s) 162, 249, 252, 255, 336, 339, 607
FokI GGATG 5 cut(s) 378, 575, 799, 812, 826
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 7 cut(s) 162, 249, 252, 255, 336, 339, 607
FspBI CTAG 4 cut(s) 192, 534, 620, 734
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 1 cut(s) 600
GluI GCNGC 7 cut(s) 162, 249, 252, 255, 336, 339, 607
GsaI CCCAGC 2 cut(s) 169, 309
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 5 cut(s) 161, 174, 206, 251, 370
HgaI GACGC 1 cut(s) 534
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 526
Hin1II CATG 3 cut(s) 49, 698, 850
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HincII GTYRAC 2 cut(s) 51, 589
HindII GTYRAC 2 cut(s) 51, 589
HinfI GANTC 6 cut(s) 92, 110, 139, 196, 326, 843
HpaI GTTAAC 1 cut(s) 589
HphI GGTGA 2 cut(s) 464, 565
Hpy166II GTNNAC 2 cut(s) 51, 589
Hpy188I TCNGA 7 cut(s) 88, 115, 201, 380, 445, 578, 859
Hpy188III TCNNGA 4 cut(s) 15, 494, 689, 847
Hpy8I GTNNAC 2 cut(s) 51, 589
Hpy99I CGWCG 3 cut(s) 247, 271, 274
HpyAV CCTTC 6 cut(s) 151, 469, 544, 573, 577, 770
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 676
HpyCH4V TGCA 5 cut(s) 4, 506, 572, 659, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 347, 534
HpyF3I CTNAG 7 cut(s) 87, 137, 237, 312, 513, 839, 858
HpySE526I ACGT 1 cut(s) 676
Hsp92I GRCGYC 1 cut(s) 526
Hsp92II CATG 3 cut(s) 49, 698, 850
HspAI GCGC 1 cut(s) 599
KflI GGGWCCC 1 cut(s) 650
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 1 cut(s) 740
LmnI GCTCC 2 cut(s) 542, 691
Lsp1109I GCAGC 2 cut(s) 241, 618
LweI GCATC 1 cut(s) 777
MaeI CTAG 4 cut(s) 192, 534, 620, 734
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 3 cut(s) 37, 298, 479
MalI GATC 1 cut(s) 742
MboI GATC 1 cut(s) 740
MboII GAAGA 8 cut(s) 75, 252, 255, 267, 270, 411, 779, 844
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 4 cut(s) 184, 394, 668, 728
MlyI GAGTC 2 cut(s) 119, 148
MroXI GAANNNNTTC 1 cut(s) 394
MseI TTAA 2 cut(s) 438, 588
MspA1I CMGCKG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 647
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 3 cut(s) 254, 347, 534
NdeII GATC 1 cut(s) 740
NlaIII CATG 3 cut(s) 49, 698, 850
NlaIV GGNNCC 3 cut(s) 349, 651, 652
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 37
NotI GCGGCCGC 1 cut(s) 249
NsbI TGCGCA 1 cut(s) 600
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 1 cut(s) 846
PciI ACATGT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 394
PfeI GAWTC 4 cut(s) 92, 196, 326, 843
PflFI GACNNNGTC 1 cut(s) 55
PkrI GCNGC 7 cut(s) 163, 250, 253, 256, 337, 340, 608
PleI GAGTC 2 cut(s) 118, 147
PpsI GAGTC 2 cut(s) 118, 147
PpuMI RGGWCCY 1 cut(s) 650
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 2 cut(s) 165, 305
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 3 cut(s) 349, 651, 652
PspPI GGNCC 2 cut(s) 173, 650
PspPPI RGGWCCY 1 cut(s) 650
PspXI VCTCGAGB 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 428
PsyI GACNNNGTC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 438, 588
SatI GCNGC 7 cut(s) 162, 249, 252, 255, 336, 339, 607
Sau3AI GATC 1 cut(s) 740
Sau96I GGNCC 2 cut(s) 173, 650
SchI GAGTC 2 cut(s) 119, 148
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 777
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 650
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 4 cut(s) 184, 394, 668, 728
SsiI CCGC 7 cut(s) 147, 153, 162, 248, 252, 335, 338
SspMI CTAG 4 cut(s) 192, 534, 620, 734
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 645
StyI CCWWGG 3 cut(s) 207, 399, 806
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 679
TaqI TCGA 2 cut(s) 50, 120
TasI AATT 4 cut(s) 184, 394, 668, 728
TauI GCSGC 5 cut(s) 164, 251, 254, 338, 341
TfiI GAWTC 4 cut(s) 92, 196, 326, 843
Tru1I TTAA 2 cut(s) 438, 588
Tru9I TTAA 2 cut(s) 438, 588
TscAI CASTG 2 cut(s) 295, 433
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 2 cut(s) 254, 606
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 395, 407, 627, 780
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 295, 433
Tth111I GACNNNGTC 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 394
XspI CTAG 4 cut(s) 192, 534, 620, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.