Rroxscaffold_7G00182150

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009009
Physical Location & Seq
Forward (+)
21169493 .. 21172381
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182150.1

Sequence Viewer

Length: 963 bp
ATGCAGGGTTTTTCTGGAGGTTTAGGAGCTGGGTCTGTGACTAACATGTCGACCGTGTCCAAAGATGAGAACTTTGCTATGTCTTCTGAGGATTCTTGTAGCCCAGATGAGTCTGAGCTCGAGCTCTGTCTTGGGCTGAGTCTCGGCGGTGGCGGGAAGGCGGCCCAGCAAGGCCCAGTTGGGAATTATGCTAGGATTCTCACGGCCAAGGATTTCCGTTATGTGGGTTCTAACTCTAAGTCGTCGGCGGCGGCGGCTGCTTCTTCGTCGTCGTCGTCGTCTTCTTCGTTGAGCAGTGCTAATGTTACGGCTGGGACTAAGAGGAGTGCTGATTCTGTGGCGGCGGCTAATGGTGCCAACAGTCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAATTCCTTGGTTCATCAAGCAAAATCTTCAGCCACTGAAGTATTCAACTCGGAGAAAGGTGAATGCAAGATTATTTCGGGGAAGGCTAACATTGGTGGTCAAGAAAACAATGCAAATGCGAAGGAGAGAGGACGCCTCACTAGTTCCCTGTTTGTGAAGGTGAATATGGATGGAAGTCCTATCGGAAGGAAGGTTAACCTAACTGCGCATAGCAGCTATGAAGCCCTAGCACAAACGTTGGAGGATATGTTCCAGGGACCCTCTGCAAATGCGAAATTGATACGTTCTGGTGGTCAGGAGCATGATATGATGGTGGGAGAAACTACACCCTCAAAATTGCTAGATGGATCATTTGATTTTGCACTCACTTATGAAGACAAAGATGGTGACTGGATGCTTGTTGGGGATGTTCCTTGGGGGATGTTTCTTTGTTCTGTGAAGAGGCTAAGAATCATGAGGACATCTGAGGCTAATGGACTTGGTATCTCCAAGGCTGCAGGAAAGGAATGCAAGGCAGAGATGCGCGAGCTGATACAAAACCATTTGTCAGAACTTCAGTAA

Protein Analysis

320

Amino Acids

33.58

Weight (kDa)

6.15

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 39 - 295 3.1e-63 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
Acc16I TGCGCA 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 927
AciI CCGC 8 cut(s) 147, 153, 161, 248, 251, 254, 341, 344
AclI AACGTT 1 cut(s) 638
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 3 cut(s) 414, 459, 941
AcyI GRCGYC 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 1 cut(s) 448
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 1 cut(s) 654
AloI GAACNNNNNNTCC 2 cut(s) 635, 667
AluBI AGCT 6 cut(s) 29, 118, 124, 392, 618, 931
AluI AGCT 6 cut(s) 29, 118, 124, 392, 618, 931
Alw21I GWGCWC 2 cut(s) 120, 126
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 757
AlwNI CAGNNNCTG 1 cut(s) 437
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 4 cut(s) 162, 172, 204, 377
ApeKI GCWGC 3 cut(s) 257, 615, 896
ApoI RAATTY 1 cut(s) 403
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 2 cut(s) 610, 927
AspS9I GGNCC 3 cut(s) 163, 173, 659
AsuHPI GGTGA 3 cut(s) 473, 574, 800
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 659
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 2 cut(s) 120, 126
BbsI GAAGAC 3 cut(s) 75, 273, 783
Bbv12I GWGCWC 2 cut(s) 120, 126
BbvI GCAGC 3 cut(s) 244, 627, 883
BccI CCATC 5 cut(s) 369, 566, 706, 740, 779
BceAI ACGGC 2 cut(s) 219, 324
BciT130I CCWGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 146
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 4 cut(s) 192, 543, 629, 743
BfmI CTRYAG 1 cut(s) 897
BisI GCNGC 9 cut(s) 162, 249, 252, 255, 258, 342, 345, 616, 897
BlsI GCNGC 9 cut(s) 163, 250, 253, 256, 259, 343, 346, 617, 898
Bme1390I CCNGG 1 cut(s) 656
Bme18I GGWCC 1 cut(s) 659
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 163, 173, 659
BmiI GGNNCC 3 cut(s) 355, 660, 661
BmrFI CCNGG 1 cut(s) 656
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 2 cut(s) 786, 912
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 75, 273, 783
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 535
BsaJI CCNNGG 5 cut(s) 207, 408, 655, 815, 891
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 176, 797
BseBI CCWGG 1 cut(s) 656
BseDI CCNNGG 5 cut(s) 207, 408, 655, 815, 891
BseGI GGATG 5 cut(s) 380, 577, 801, 814, 828
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 4 cut(s) 78, 105, 128, 858
BseNI ACTGG 2 cut(s) 176, 797
BseRI GAGGAG 1 cut(s) 337
BseXI GCAGC 3 cut(s) 244, 627, 883
BseYI CCCAGC 3 cut(s) 29, 165, 311
Bsh1236I CGCG 1 cut(s) 927
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 164, 174, 206, 379
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 2 cut(s) 120, 126
BsiHKCI CYCGRG 1 cut(s) 119
BslFI GGGAC 2 cut(s) 328, 672
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 328, 672
BsmI GAATGC 2 cut(s) 470, 914
BsnI GGCC 4 cut(s) 164, 174, 206, 379
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 120, 126
Bsp143I GATC 1 cut(s) 749
BspACI CCGC 8 cut(s) 147, 153, 161, 248, 251, 254, 341, 344
BspANI GGCC 4 cut(s) 164, 174, 206, 379
BspCNI CTCAG 4 cut(s) 79, 106, 129, 859
BspFNI CGCG 1 cut(s) 927
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 355, 660, 661
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 353
BsrI ACTGG 2 cut(s) 176, 797
BssECI CCNNGG 5 cut(s) 207, 408, 655, 815, 891
BssMI GATC 1 cut(s) 749
BssNI GRCGYC 1 cut(s) 535
BssT1I CCWWGG 4 cut(s) 207, 408, 815, 891
Bst2UI CCWGG 1 cut(s) 656
Bst4CI ACNGT 2 cut(s) 55, 362
Bst6I CTCTTC 1 cut(s) 836
BstACI GRCGYC 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 929
BstDEI CTNAG 7 cut(s) 87, 114, 137, 237, 318, 848, 867
BstF5I GGATG 5 cut(s) 380, 577, 801, 814, 828
BstFNI CGCG 1 cut(s) 927
BstHHI GCGC 2 cut(s) 610, 927
BstKTI GATC 1 cut(s) 752
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 1 cut(s) 749
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 254, 257, 353
BstNI CCWGG 1 cut(s) 656
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 654
BstSFI CTRYAG 1 cut(s) 897
BstUI CGCG 1 cut(s) 927
BstV1I GCAGC 3 cut(s) 244, 627, 883
BstV2I GAAGAC 3 cut(s) 75, 273, 783
BsuRI GGCC 4 cut(s) 164, 174, 206, 379
BtsCI GGATG 5 cut(s) 380, 577, 801, 814, 828
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 301, 435
Cac8I GCNNGC 1 cut(s) 929
CaiI CAGNNNCTG 1 cut(s) 437
CciI TCATGA 1 cut(s) 855
CfoI GCGC 2 cut(s) 610, 927
Cfr13I GGNCC 3 cut(s) 163, 173, 659
CseI GACGC 1 cut(s) 543
CviAII CATG 3 cut(s) 46, 704, 856
DdeI CTNAG 7 cut(s) 87, 114, 137, 237, 318, 848, 867
DpnI GATC 1 cut(s) 751
DpnII GATC 1 cut(s) 749
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 836
EarI CTCTTC 1 cut(s) 836
Ecl136II GAGCTC 2 cut(s) 118, 124
Eco130I CCWWGG 4 cut(s) 207, 408, 815, 891
Eco24I GRGCYC 2 cut(s) 120, 126
Eco47I GGWCC 1 cut(s) 659
Eco53kI GAGCTC 2 cut(s) 118, 124
Eco57I CTGAAG 3 cut(s) 414, 459, 941
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 2 cut(s) 118, 124
EcoO109I RGGNCCY 1 cut(s) 659
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 654
EcoT14I CCWWGG 4 cut(s) 207, 408, 815, 891
EcoT38I GRGCYC 2 cut(s) 120, 126
ErhI CCWWGG 4 cut(s) 207, 408, 815, 891
FaeI CATG 3 cut(s) 49, 707, 859
FaqI GGGAC 2 cut(s) 328, 672
FatI CATG 3 cut(s) 45, 703, 855
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 9 cut(s) 162, 249, 252, 255, 258, 342, 345, 616, 897
FokI GGATG 5 cut(s) 387, 584, 808, 821, 835
FriOI GRGCYC 2 cut(s) 120, 126
Fsp4HI GCNGC 9 cut(s) 162, 249, 252, 255, 258, 342, 345, 616, 897
FspBI CTAG 4 cut(s) 192, 543, 629, 743
FspI TGCGCA 1 cut(s) 609
GlaI GCGC 2 cut(s) 609, 926
GluI GCNGC 9 cut(s) 162, 249, 252, 255, 258, 342, 345, 616, 897
GsaI CCCAGC 3 cut(s) 33, 169, 315
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 4 cut(s) 164, 174, 206, 379
HgaI GACGC 1 cut(s) 543
HhaI GCGC 2 cut(s) 610, 927
Hin1I GRCGYC 1 cut(s) 535
Hin1II CATG 3 cut(s) 49, 707, 859
Hin6I GCGC 2 cut(s) 608, 925
HinP1I GCGC 2 cut(s) 608, 925
HincII GTYRAC 2 cut(s) 51, 598
HindII GTYRAC 2 cut(s) 51, 598
HinfI GANTC 6 cut(s) 92, 110, 139, 196, 332, 852
HpaI GTTAAC 1 cut(s) 598
HphI GGTGA 3 cut(s) 473, 574, 800
Hpy166II GTNNAC 2 cut(s) 51, 598
Hpy188I TCNGA 7 cut(s) 88, 115, 389, 454, 587, 868, 952
Hpy188III TCNNGA 4 cut(s) 15, 503, 698, 856
Hpy8I GTNNAC 2 cut(s) 51, 598
Hpy99I CGWCG 5 cut(s) 247, 271, 274, 277, 280
HpyAV CCTTC 6 cut(s) 151, 478, 517, 553, 582, 586
HpyCH4III ACNGT 2 cut(s) 55, 362
HpyCH4IV ACGT 2 cut(s) 638, 685
HpyCH4V TGCA 7 cut(s) 4, 468, 515, 668, 764, 899, 912
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 257, 353
HpyF3I CTNAG 7 cut(s) 87, 114, 137, 237, 318, 848, 867
HpySE526I ACGT 2 cut(s) 638, 685
Hsp92I GRCGYC 1 cut(s) 535
Hsp92II CATG 3 cut(s) 49, 707, 859
HspAI GCGC 2 cut(s) 608, 925
KflI GGGWCCC 1 cut(s) 659
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 1 cut(s) 749
LmnI GCTCC 2 cut(s) 26, 700
Lsp1109I GCAGC 3 cut(s) 244, 627, 883
LweI GCATC 2 cut(s) 786, 912
MaeI CTAG 4 cut(s) 192, 543, 629, 743
MaeII ACGT 2 cut(s) 638, 685
MaeIII GTNAC 3 cut(s) 37, 304, 788
MalI GATC 1 cut(s) 751
MboI GATC 1 cut(s) 749
MboII GAAGA 7 cut(s) 75, 255, 273, 276, 420, 788, 853
MhlI GDGCHC 2 cut(s) 120, 126
MluCI AATT 4 cut(s) 184, 403, 677, 737
MlyI GAGTC 2 cut(s) 119, 148
MmeI TCCRAC 1 cut(s) 621
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 1 cut(s) 597
MspR9I CCNGG 1 cut(s) 656
Mva1269I GAATGC 2 cut(s) 470, 914
MvaI CCWGG 1 cut(s) 656
MvnI CGCG 1 cut(s) 927
MwoI GCNNNNNNNGC 3 cut(s) 254, 257, 353
NdeII GATC 1 cut(s) 749
NlaIII CATG 3 cut(s) 49, 707, 859
NlaIV GGNNCC 3 cut(s) 355, 660, 661
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 37, 788
NsbI TGCGCA 1 cut(s) 609
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 1 cut(s) 855
PciI ACATGT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 3 cut(s) 272, 275, 284
PctI GAATGC 2 cut(s) 470, 914
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 4 cut(s) 92, 196, 332, 852
PflFI GACNNNGTC 1 cut(s) 55
PkrI GCNGC 9 cut(s) 163, 250, 253, 256, 259, 343, 346, 617, 898
PleI GAGTC 2 cut(s) 118, 147
PpsI GAGTC 2 cut(s) 118, 147
PpuMI RGGWCCY 1 cut(s) 659
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 2 cut(s) 120, 126
Psp1406I AACGTT 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 654
PspFI CCCAGC 3 cut(s) 29, 165, 311
PspGI CCWGG 1 cut(s) 654
PspN4I GGNNCC 3 cut(s) 355, 660, 661
PspPI GGNCC 3 cut(s) 163, 173, 659
PspPPI RGGWCCY 1 cut(s) 659
PspXI VCTCGAGB 1 cut(s) 119
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 55
SacI GAGCTC 2 cut(s) 120, 126
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 9 cut(s) 162, 249, 252, 255, 258, 342, 345, 616, 897
Sau3AI GATC 1 cut(s) 749
Sau96I GGNCC 3 cut(s) 163, 173, 659
SchI GAGTC 2 cut(s) 119, 148
ScrFI CCNGG 1 cut(s) 656
SduI GDGCHC 2 cut(s) 120, 126
SfaNI GCATC 2 cut(s) 786, 912
SfcI CTRYAG 1 cut(s) 897
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 659
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 4 cut(s) 184, 403, 677, 737
SsiI CCGC 8 cut(s) 147, 153, 161, 248, 251, 254, 341, 344
SspMI CTAG 4 cut(s) 192, 543, 629, 743
SstI GAGCTC 2 cut(s) 120, 126
StyD4I CCNGG 1 cut(s) 654
StyI CCWWGG 4 cut(s) 207, 408, 815, 891
TaaI ACNGT 2 cut(s) 55, 362
TaiI ACGT 2 cut(s) 641, 688
TaqI TCGA 2 cut(s) 50, 120
TasI AATT 4 cut(s) 184, 403, 677, 737
TauI GCSGC 6 cut(s) 164, 251, 254, 257, 344, 347
TfiI GAWTC 4 cut(s) 92, 196, 332, 852
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 2 cut(s) 301, 442
TseFI GTSAC 2 cut(s) 37, 788
TseI GCWGC 3 cut(s) 257, 615, 896
Tsp45I GTSAC 2 cut(s) 37, 788
TspDTI ATGAA 4 cut(s) 404, 416, 636, 789
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 301, 442
Tth111I GACNNNGTC 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 1 cut(s) 403
XceI RCATGY 1 cut(s) 49
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 403
XspI CTAG 4 cut(s) 192, 543, 629, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.