MD10G1176400.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009009
Physical Location & Seq
Reverse (-)
26870201 .. 26873035
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1176400.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGAGAGTGGAGGTTCTGCAAGTGGGTCGTTGACTAAGTCAACGCTGTCCAGGGAGGAGAATTTTGCCATGTCTTCTGAGGACTCTTCTACCCCTGAGGAGTCTGGCCTCGAGCTCTGTCTTGGGCTGAGCCTTGGTGGTGGTGGAGGGAAGGATCAGCAGGGGCAAAGGGGTCACTTTGCGAGGATCTTGACTGCTAAGGATTTTCCTTCTGTGGGGTTTTCTTCTTCGTCGGCTTCCGAGTCGTCGTCTTCTACTTCTTCGTTGAGCAGCGGTAATGTTGCTGCTGGAACCAAGAGAAGTGCTGATTCTGTGGCTGCTGCTAATGGTGCCAGTCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAATAGTTTGGTTCTCCAAGCGAAATCTTCATCCACTGAAGGACTTAACTCAGTAAATGAGAAAAGTGAATACAAGACCGGTGCAGAGAAGGTTAACAATGGAGGTCACAAGTCCAATGGAAATGCTAAGGAGATAGGGCAGCAGAGGGGTTCACTGTTTGTGAAGGTGAATATGGACGGGATTCCTATTGGGCGAAAGGTTAATCTAAGTGCGCATAGTTCCTATGAAGCGCTAGCACAAAAATTGGAGGATATGTTTGGGCCCTCGACACATGGTTCAGGTGGTCAGGAGATGGAAGGAGCAACAAGACCCTCAAAGTTGCTGGATGGATCATTTGAGTTTGCGCTCACTTATGAAGATAAAGATGGAGACTGGATGCTTGTGGGAGATGTTCCTTGGGAGATGTTTCTTGGTACTGTCAAGAGGTTAAGGATTATGAGGACATCTGAGGCTAACGGACTTGCTCCCCTGTTACAGGAAAAGAATGTGAGGCAAAGATGTAAGCCAACCTAG

Protein Analysis

296

Amino Acids

31.09

Weight (kDa)

6.47

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 38 - 281 4.9e-61 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 329
AciI CCGC 1 cut(s) 273
AclWI GGATC 3 cut(s) 162, 194, 712
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 1 cut(s) 790
AfeI AGCGCT 1 cut(s) 606
AfiI CCNNNNNNNGG 2 cut(s) 215, 850
AgeI ACCGGT 1 cut(s) 452
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 2 cut(s) 115, 365
AluI AGCT 2 cut(s) 115, 365
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 738
AlwI GGATC 3 cut(s) 162, 194, 712
Ama87I CYCGRG 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 606
AoxI GGCC 3 cut(s) 106, 350, 635
ApaI GGGCCC 1 cut(s) 639
ApeKI GCWGC 5 cut(s) 270, 284, 317, 320, 514
ApoI RAATTY 1 cut(s) 61
AsiGI ACCGGT 1 cut(s) 452
AspLEI GCGC 3 cut(s) 589, 607, 721
AspS9I GGNCC 2 cut(s) 635, 636
AsuHPI GGTGA 1 cut(s) 553
AsuNHI GCTAGC 1 cut(s) 607
AvaI CYCGRG 1 cut(s) 110
AxyI CCTNAGG 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 639
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 2 cut(s) 117, 639
BbsI GAAGAC 2 cut(s) 66, 243
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 5 cut(s) 271, 282, 304, 307, 526
BccI CCATC 4 cut(s) 342, 661, 695, 734
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BciT130I CCWGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 738
BfaI CTAG 2 cut(s) 608, 886
BfoI RGCGCY 1 cut(s) 608
BisI GCNGC 5 cut(s) 271, 285, 318, 321, 515
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 5 cut(s) 272, 286, 319, 322, 516
Bme1390I CCNGG 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 635, 636
BmiI GGNNCC 3 cut(s) 292, 331, 637
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 741
BmtI GCTAGC 1 cut(s) 611
BpiI GAAGAC 2 cut(s) 66, 243
Bpu10I CCTNAGC 2 cut(s) 198, 501
Bpu1102I GCTNAGC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 51, 133, 770
BsaWI WCCGGW 1 cut(s) 452
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 850
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 2 cut(s) 333, 752
Bse21I CCTNAGG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 3 cut(s) 51, 133, 770
BseGI GGATG 4 cut(s) 353, 404, 706, 756
BseLI CCNNNNNNNGG 2 cut(s) 215, 850
BseMII CTCAG 5 cut(s) 69, 87, 119, 438, 813
BseNI ACTGG 2 cut(s) 333, 752
BseRI GAGGAG 2 cut(s) 71, 113
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 5 cut(s) 271, 282, 304, 307, 526
BsgI GTGCAG 1 cut(s) 477
BshFI GGCC 3 cut(s) 108, 352, 637
BshNI GGYRCC 1 cut(s) 329
BshTI ACCGGT 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 453
BslI CCNNNNNNNGG 2 cut(s) 215, 850
BsmAI GTCTC 1 cut(s) 738
BsnI GGCC 3 cut(s) 108, 352, 637
BsoBI CYCGRG 1 cut(s) 110
Bsp120I GGGCCC 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 117, 639
Bsp143I GATC 3 cut(s) 154, 186, 704
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 1 cut(s) 273
BspANI GGCC 3 cut(s) 108, 352, 637
BspCNI CTCAG 5 cut(s) 70, 88, 120, 437, 814
BspLI GGNNCC 3 cut(s) 292, 331, 637
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 162, 194, 712
BspT107I GGYRCC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 2 cut(s) 333, 752
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 3 cut(s) 51, 133, 770
BssMI GATC 3 cut(s) 154, 186, 704
BssT1I CCWWGG 2 cut(s) 133, 770
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 2 cut(s) 531, 793
Bst6I CTCTTC 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 9 cut(s) 36, 78, 96, 128, 198, 424, 501, 581, 822
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 4 cut(s) 353, 404, 706, 756
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 3 cut(s) 589, 607, 721
BstKTI GATC 3 cut(s) 157, 189, 707
BstMAI GTCTC 1 cut(s) 738
BstMBI GATC 3 cut(s) 154, 186, 704
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstSLI GKGCMC 1 cut(s) 639
BstV1I GCAGC 5 cut(s) 271, 282, 304, 307, 526
BstV2I GAAGAC 2 cut(s) 66, 243
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 3 cut(s) 108, 352, 637
BtsCI GGATG 4 cut(s) 353, 404, 706, 756
BtsIMutI CAGTG 2 cut(s) 408, 527
Cac8I GCNNGC 1 cut(s) 609
CfoI GCGC 3 cut(s) 589, 607, 721
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 635, 636
Csp6I GTAC 1 cut(s) 789
CspAI ACCGGT 1 cut(s) 452
CviAII CATG 2 cut(s) 70, 647
CviQI GTAC 1 cut(s) 789
DdeI CTNAG 9 cut(s) 36, 78, 96, 128, 198, 424, 501, 581, 822
DpnI GATC 3 cut(s) 156, 188, 706
DpnII GATC 3 cut(s) 154, 186, 704
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 133, 770
Eco24I GRGCYC 2 cut(s) 117, 639
Eco47III AGCGCT 1 cut(s) 606
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoO109I RGGNCCY 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 2 cut(s) 133, 770
EcoT38I GRGCYC 2 cut(s) 117, 639
ErhI CCWWGG 2 cut(s) 133, 770
FaeI CATG 2 cut(s) 73, 650
FaiI YATR 9 cut(s) 71, 369, 548, 591, 600, 629, 648, 729, 812
FatI CATG 2 cut(s) 69, 646
Fnu4HI GCNGC 5 cut(s) 271, 285, 318, 321, 515
FokI GGATG 4 cut(s) 360, 391, 713, 763
FriOI GRGCYC 2 cut(s) 117, 639
Fsp4HI GCNGC 5 cut(s) 271, 285, 318, 321, 515
FspAI RTGCGCAY 1 cut(s) 588
FspBI CTAG 2 cut(s) 608, 886
FspI TGCGCA 1 cut(s) 588
GlaI GCGC 3 cut(s) 588, 606, 720
GluI GCNGC 5 cut(s) 271, 285, 318, 321, 515
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 3 cut(s) 108, 352, 637
HapII CCGG 1 cut(s) 453
HhaI GCGC 3 cut(s) 589, 607, 721
Hin1II CATG 2 cut(s) 73, 650
Hin6I GCGC 3 cut(s) 587, 605, 719
HinP1I GCGC 3 cut(s) 587, 605, 719
HincII GTYRAC 3 cut(s) 33, 42, 469
HindII GTYRAC 3 cut(s) 33, 42, 469
HinfI GANTC 5 cut(s) 83, 101, 242, 308, 556
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 1 cut(s) 453
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 4 cut(s) 33, 42, 469, 527
Hpy188I TCNGA 4 cut(s) 79, 241, 362, 823
Hpy188III TCNNGA 3 cut(s) 190, 662, 796
Hpy8I GTNNAC 4 cut(s) 33, 42, 469, 527
Hpy99I CGWCG 2 cut(s) 235, 250
HpyAV CCTTC 6 cut(s) 145, 219, 407, 457, 532, 665
HpyCH4III ACNGT 2 cut(s) 531, 793
HpyCH4V TGCA 2 cut(s) 20, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 9 cut(s) 36, 78, 96, 128, 198, 424, 501, 581, 822
Hsp92II CATG 2 cut(s) 73, 650
HspAI GCGC 3 cut(s) 587, 605, 719
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 3 cut(s) 154, 186, 704
LmnI GCTCC 2 cut(s) 674, 844
Lsp1109I GCAGC 5 cut(s) 271, 282, 304, 307, 526
LweI GCATC 1 cut(s) 741
MaeI CTAG 2 cut(s) 608, 886
MaeIII GTNAC 3 cut(s) 173, 479, 846
MalI GATC 3 cut(s) 156, 188, 706
MboI GATC 3 cut(s) 154, 186, 704
MboII GAAGA 8 cut(s) 66, 78, 216, 219, 243, 252, 393, 743
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 2 cut(s) 117, 639
MluCI AATT 2 cut(s) 61, 617
MlyI GAGTC 3 cut(s) 77, 110, 251
MseI TTAA 4 cut(s) 420, 468, 576, 803
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeII GATC 3 cut(s) 154, 186, 704
NheI GCTAGC 1 cut(s) 607
NlaIII CATG 2 cut(s) 73, 650
NlaIV GGNNCC 3 cut(s) 292, 331, 637
NmuCI GTSAC 2 cut(s) 173, 479
NsbI TGCGCA 1 cut(s) 588
PaeR7I CTCGAG 1 cut(s) 110
PfeI GAWTC 2 cut(s) 308, 556
PflFI GACNNNGTC 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 5 cut(s) 272, 286, 319, 322, 516
PleI GAGTC 3 cut(s) 77, 109, 250
PpsI GAGTC 3 cut(s) 77, 109, 250
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 3 cut(s) 292, 331, 637
PspOMI GGGCCC 1 cut(s) 635
PspPI GGNCC 2 cut(s) 635, 636
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 1 cut(s) 186
PsyI GACNNNGTC 1 cut(s) 37
RsaI GTAC 1 cut(s) 790
RsaNI GTAC 1 cut(s) 789
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 4 cut(s) 420, 468, 576, 803
SatI GCNGC 5 cut(s) 271, 285, 318, 321, 515
Sau3AI GATC 3 cut(s) 154, 186, 704
Sau96I GGNCC 2 cut(s) 635, 636
SchI GAGTC 3 cut(s) 77, 110, 251
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 117, 639
SfaNI GCATC 1 cut(s) 741
Sfr274I CTCGAG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 2 cut(s) 61, 617
SsiI CCGC 1 cut(s) 273
SspMI CTAG 2 cut(s) 608, 886
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 2 cut(s) 133, 770
TaaI ACNGT 2 cut(s) 531, 793
TaqI TCGA 2 cut(s) 111, 641
TasI AATT 2 cut(s) 61, 617
TfiI GAWTC 2 cut(s) 308, 556
Tru1I TTAA 4 cut(s) 420, 468, 576, 803
Tru9I TTAA 4 cut(s) 420, 468, 576, 803
TscAI CASTG 2 cut(s) 415, 534
TseFI GTSAC 2 cut(s) 173, 479
TseI GCWGC 5 cut(s) 270, 284, 317, 320, 514
Tsp45I GTSAC 2 cut(s) 173, 479
TspDTI ATGAA 4 cut(s) 389, 393, 615, 744
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 2 cut(s) 415, 534
Tth111I GACNNNGTC 1 cut(s) 37
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 2 cut(s) 608, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.