pycom05g17340

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009009
Physical Location & Seq
Reverse (-)
20516596 .. 20518691
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17340.1

Sequence Viewer

Length: 897 bp
ATGGAGAGTGGAGGTTCTGCAAGTGGGTCGTTGACTAAGTCAACGCTGTCCAGGGAGGAGAATTTTGCCATGTCTTCTGAGGACTCTTCTACCCCTGAGGAGTCTGGGCTCGAGCTCTGTCTTGGGCTGAGCCTTGGTGGTGGTGGTGGTGGTGGTAAGGGTCAGCAAGGGCAAAGGGGTCAAGTTGCTAGGATCTTGACTGCTAAGGATTTTCCTTCTGTGGGTTTTTCTTCTTCGTCGGCTTCCAAGTCGTCGTCTTCTACTTCTTCGTTGAGCAGTGCTAATGTTGCGGCTGGAACCAAGAGAAGTGCTGATTCTGTGGCTGCTGCTAATGGTGGCAGTCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAACAGTTTGGTTCACCAAGCGAAATCTTCATCTGCCGAAGGATCTAACTCGGTAAACGAGAGAAGCGAGTGCAAGACTGGTGTGGGGAAGGTTAACAATGGAGGTCACAAGACCAATGGAAATGCTAAGGAGATAGGGCAGCAGAGGGGTTCCCTGTTTGTGAAGGTGAATATGGACGGGATTCCTATTGGGCGAAAGGTTAATCTAAATGCACATAGTTCGTATGAAGCCCTAGCACAAAAATTGGAGGATATGTTCCATGGGCCCTCGACACATGGTTCAGGTGGTCAGGAGATGGAAGGAGAAACTAGACCCTCAAAGTTGCTGGATCGATCGTTTGAGTTTGCGCTCACTTATGAAGATAAAGATGGAGACTGGATGCTTGTGGGAGACGTTCCTTGGGAGATGTTTCTTGGTACCGTGAAGAGGTTAAGGATTATGAGGACATCTGAGGATAACGGACTCGCTCCGCTGTTACGGGAAAAGAATGTGAGGCAAAGATGTAAGCCAATCTAG

Protein Analysis

299

Amino Acids

31.39

Weight (kDa)

8.7

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 38 - 284 1.9e-61 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 797
AciI CCGC 2 cut(s) 290, 851
AclWI GGATC 3 cut(s) 200, 430, 717
AcsI RAATTY 1 cut(s) 61
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 2 cut(s) 221, 807
AjnI CCWGG 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 620, 652
AluBI AGCT 2 cut(s) 115, 371
AluI AGCT 2 cut(s) 115, 371
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 747, 765
AlwI GGATC 3 cut(s) 200, 430, 717
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 356, 644
ApaI GGGCCC 1 cut(s) 648
ApeKI GCWGC 3 cut(s) 323, 326, 520
ApoI RAATTY 1 cut(s) 61
Asp718I GGTACC 1 cut(s) 797
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 2 cut(s) 644, 645
AsuHPI GGTGA 2 cut(s) 386, 559
AvaI CYCGRG 1 cut(s) 110
AxyI CCTNAGG 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 648
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 3 cut(s) 111, 117, 648
BbsI GAAGAC 2 cut(s) 66, 249
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 310, 313, 532
BccI CCATC 3 cut(s) 348, 670, 743
BcgI CGANNNNNNTGC 4 cut(s) 9, 43, 582, 616
BciT130I CCWGG 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 747, 765
BfaI CTAG 4 cut(s) 189, 614, 690, 895
BisI GCNGC 4 cut(s) 291, 324, 327, 521
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 4 cut(s) 292, 325, 328, 522
Bme1390I CCNGG 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 644, 645
BmiI GGNNCC 4 cut(s) 298, 532, 646, 799
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 750
BpiI GAAGAC 2 cut(s) 66, 249
Bpu10I CCTNAGC 2 cut(s) 204, 507
Bpu1102I GCTNAGC 1 cut(s) 128
Bsa29I ATCGAT 1 cut(s) 712
BsaJI CCNNGG 4 cut(s) 51, 133, 640, 779
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 807
Bse1I ACTGG 2 cut(s) 463, 761
Bse21I CCTNAGG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 712
BseDI CCNNGG 4 cut(s) 51, 133, 640, 779
BseGI GGATG 2 cut(s) 359, 765
BseLI CCNNNNNNNGG 2 cut(s) 221, 807
BseMII CTCAG 4 cut(s) 69, 87, 119, 822
BseNI ACTGG 2 cut(s) 463, 761
BseRI GAGGAG 2 cut(s) 71, 113
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 3 cut(s) 310, 313, 532
Bsh1285I CGRYCG 1 cut(s) 716
BshFI GGCC 2 cut(s) 358, 646
BshNI GGYRCC 1 cut(s) 797
BshVI ATCGAT 1 cut(s) 712
BsiEI CGRYCG 1 cut(s) 716
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 221, 807
BsmAI GTCTC 2 cut(s) 747, 765
BsmBI CGTCTC 1 cut(s) 765
BsnI GGCC 2 cut(s) 358, 646
BsoBI CYCGRG 1 cut(s) 110
Bsp120I GGGCCC 1 cut(s) 644
Bsp1286I GDGCHC 3 cut(s) 111, 117, 648
Bsp143I GATC 4 cut(s) 192, 422, 709, 713
Bsp1720I GCTNAGC 1 cut(s) 128
Bsp19I CCATGG 1 cut(s) 640
BspACI CCGC 2 cut(s) 290, 851
BspANI GGCC 2 cut(s) 358, 646
BspCNI CTCAG 4 cut(s) 70, 88, 120, 823
BspDI ATCGAT 1 cut(s) 712
BspLI GGNNCC 4 cut(s) 298, 532, 646, 799
BspPI GGATC 3 cut(s) 200, 430, 717
BspT107I GGYRCC 1 cut(s) 797
BsrI ACTGG 2 cut(s) 463, 761
BssECI CCNNGG 4 cut(s) 51, 133, 640, 779
BssMI GATC 4 cut(s) 192, 422, 709, 713
BssT1I CCWWGG 3 cut(s) 133, 640, 779
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 2 cut(s) 386, 802
Bst6I CTCTTC 2 cut(s) 91, 800
BstDEI CTNAG 7 cut(s) 36, 78, 96, 128, 204, 507, 831
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 2 cut(s) 359, 765
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 4 cut(s) 195, 425, 712, 716
BstMAI GTCTC 2 cut(s) 747, 765
BstMBI GATC 4 cut(s) 192, 422, 709, 713
BstMCI CGRYCG 1 cut(s) 716
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstSLI GKGCMC 1 cut(s) 648
BstV1I GCAGC 3 cut(s) 310, 313, 532
BstV2I GAAGAC 2 cut(s) 66, 249
BstX2I RGATCY 2 cut(s) 192, 422
BstYI RGATCY 2 cut(s) 192, 422
Bsu15I ATCGAT 1 cut(s) 712
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 358, 646
BsuTUI ATCGAT 1 cut(s) 712
BtgI CCRYGG 1 cut(s) 640
BtsCI GGATG 2 cut(s) 359, 765
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 2 cut(s) 644, 645
ClaI ATCGAT 1 cut(s) 712
Csp6I GTAC 1 cut(s) 798
CviAII CATG 3 cut(s) 70, 641, 656
CviQI GTAC 1 cut(s) 798
DdeI CTNAG 7 cut(s) 36, 78, 96, 128, 204, 507, 831
DpnI GATC 4 cut(s) 194, 424, 711, 715
DpnII GATC 4 cut(s) 192, 422, 709, 713
Eam1104I CTCTTC 2 cut(s) 91, 800
EarI CTCTTC 2 cut(s) 91, 800
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 133, 640, 779
Eco24I GRGCYC 3 cut(s) 111, 117, 648
Eco53kI GAGCTC 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 3 cut(s) 133, 640, 779
EcoT38I GRGCYC 3 cut(s) 111, 117, 648
ErhI CCWWGG 3 cut(s) 133, 640, 779
Esp3I CGTCTC 1 cut(s) 765
FaeI CATG 3 cut(s) 73, 644, 659
FatI CATG 3 cut(s) 69, 640, 655
Fnu4HI GCNGC 4 cut(s) 291, 324, 327, 521
FokI GGATG 2 cut(s) 366, 772
FriOI GRGCYC 3 cut(s) 111, 117, 648
Fsp4HI GCNGC 4 cut(s) 291, 324, 327, 521
FspBI CTAG 4 cut(s) 189, 614, 690, 895
GlaI GCGC 1 cut(s) 729
GluI GCNGC 4 cut(s) 291, 324, 327, 521
HaeIII GGCC 2 cut(s) 358, 646
HhaI GCGC 1 cut(s) 730
Hin1II CATG 3 cut(s) 73, 644, 659
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 3 cut(s) 33, 42, 475
HindII GTYRAC 3 cut(s) 33, 42, 475
HinfI GANTC 5 cut(s) 83, 101, 314, 562, 843
HpaI GTTAAC 1 cut(s) 475
HphI GGTGA 2 cut(s) 386, 559
Hpy166II GTNNAC 5 cut(s) 33, 42, 394, 436, 475
Hpy188I TCNGA 3 cut(s) 79, 368, 832
Hpy188III TCNNGA 2 cut(s) 196, 671
Hpy8I GTNNAC 5 cut(s) 33, 42, 394, 436, 475
Hpy99I CGWCG 2 cut(s) 241, 256
HpyAV CCTTC 5 cut(s) 225, 413, 463, 538, 674
HpyCH4III ACNGT 2 cut(s) 386, 802
HpyCH4IV ACGT 1 cut(s) 774
HpyCH4V TGCA 3 cut(s) 20, 453, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 7 cut(s) 36, 78, 96, 128, 204, 507, 831
HpySE526I ACGT 1 cut(s) 774
Hsp92II CATG 3 cut(s) 73, 644, 659
HspAI GCGC 1 cut(s) 728
KpnI GGTACC 1 cut(s) 801
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 4 cut(s) 192, 422, 709, 713
LmnI GCTCC 1 cut(s) 853
Lsp1109I GCAGC 3 cut(s) 310, 313, 532
LweI GCATC 1 cut(s) 750
MaeI CTAG 4 cut(s) 189, 614, 690, 895
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 2 cut(s) 485, 855
MalI GATC 4 cut(s) 194, 424, 711, 715
MboI GATC 4 cut(s) 192, 422, 709, 713
MboII GAAGA 9 cut(s) 66, 78, 222, 225, 249, 258, 399, 752, 817
MflI RGATCY 2 cut(s) 192, 422
MhlI GDGCHC 3 cut(s) 111, 117, 648
MluCI AATT 2 cut(s) 61, 623
MlyI GAGTC 3 cut(s) 77, 110, 837
MseI TTAA 3 cut(s) 474, 582, 812
MspA1I CMGCKG 1 cut(s) 853
MspR9I CCNGG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 287
NcoI CCATGG 1 cut(s) 640
NdeII GATC 4 cut(s) 192, 422, 709, 713
NlaIII CATG 3 cut(s) 73, 644, 659
NlaIV GGNNCC 4 cut(s) 298, 532, 646, 799
NmuCI GTSAC 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PfeI GAWTC 2 cut(s) 314, 562
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 4 cut(s) 292, 325, 328, 522
Ple19I CGATCG 1 cut(s) 716
PleI GAGTC 3 cut(s) 77, 109, 837
PpsI GAGTC 3 cut(s) 77, 109, 837
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 4 cut(s) 298, 532, 646, 799
PspOMI GGGCCC 1 cut(s) 644
PspPI GGNCC 2 cut(s) 644, 645
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 2 cut(s) 192, 422
PsyI GACNNNGTC 1 cut(s) 37
PvuI CGATCG 1 cut(s) 716
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 474, 582, 812
SatI GCNGC 4 cut(s) 291, 324, 327, 521
Sau3AI GATC 4 cut(s) 192, 422, 709, 713
Sau96I GGNCC 2 cut(s) 644, 645
SchI GAGTC 3 cut(s) 77, 110, 837
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 3 cut(s) 111, 117, 648
SfaNI GCATC 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 2 cut(s) 61, 623
SsiI CCGC 2 cut(s) 290, 851
SspMI CTAG 4 cut(s) 189, 614, 690, 895
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 3 cut(s) 133, 640, 779
TaaI ACNGT 2 cut(s) 386, 802
TaiI ACGT 1 cut(s) 777
TaqI TCGA 3 cut(s) 111, 650, 712
TasI AATT 2 cut(s) 61, 623
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 2 cut(s) 314, 562
Tru1I TTAA 3 cut(s) 474, 582, 812
Tru9I TTAA 3 cut(s) 474, 582, 812
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 3 cut(s) 323, 326, 520
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 395, 399, 621, 753
TspGWI ACGGA 1 cut(s) 855
TspRI CASTG 1 cut(s) 283
Tth111I GACNNNGTC 1 cut(s) 37
XapI RAATTY 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 4 cut(s) 189, 614, 690, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.