AT4G29890

choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005808
Physical Location & Seq
Forward (+)
14608833 .. 14610998
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29890.1

Sequence Viewer

Length: 1269 bp
ATGATGACCACGTTAACGGCGACAGTGCCGGAATTTCTACCCCCGTCGCTAAAATCCACCAGAGGATATTTCAATTCTCATAGTGAATTTGGCGTTTCAATCTCTAAATTTAGTCGGAGAAGATTTCACAATCCGACCAGAGTCTTCGCCGTTTCAGATATCAGTAAGCTCGTGACGGAGTTTGATCCGAAGATTCCATTGGAGAGAGCATCTACTCCGCCGAGCTCGTGGTACACTGATCCTCAATTCTACAGTTTCGAGCTCGATCGTGTCTTCTATGGAGGATGGCAAGCCGTAGGCTACTCTGATCAGATTAAGGAGAGTCGTGACTTTTTCACCGGGAGGCTTGGAGATGTAGATTTTGTTGTGTGTAGAGATGAGAATGGAAAGATACATGCTTTCCATAATGTATGCTCTCACCATGCCTCGATCTTGGCTTCTGGAAATGGGAGAAAGTCTTGTTTTGTTTGCCTTTATCATGGATGGACTTATAGCTTGAGTGGATCGCTTGTCAAAGCTACTAGAATGAGTGGGATACAGAATTTTTCTTTAAGTGAAATGGGACTGAAACCTCTAAGAGTGGCTGTGTGGGGACCTTTTGTGCTTCTCAAGGTGACTGCAGCCACATCGAGGAAGGGAGAAGTTGAGACCGATGAATTAGTGGCATCAGAATGGCTTGGTACTTCCGTGGGGAGGTTGAGCCAAGGTGGAGTTGATTCCCCGCTTAGTTATATATGCAGACGTGAATACACCATTGATTGTAATTGGAAGGTGTTCTGTGATAACTACTTAGATGGTGGCTATCATGTACCTTATGCACATAAAGGTTTGATGTCGGGACTTGATCTCGAAACCTATTCTACAACGATATTCGAAAAGGTTAGTATCCAAGAATGTGGAGGTGGTTCAAAGGTTGGCGAAGACGGTTTTGATAGGCTTGGATCTGAAGCTCTATACGCGTTTGTCTACCCAAATTTCATGATAAATAGGTATGGACCTTGGATGGACACCAATCTAGTGCTACCTTTAGGACCAAGGAAATGTAAAGTTGTCTTCGATTATTTTCTTGATCCTTCTTTAAAGGATGATGAAGCTTTCATCAAGAGAAGTTTGGAAGAAAGCGATAGAGTTCAGATGGAAGATGTTATGTTGTGTGAGAGTGTTCAGAGGGGTCTCGAATCTCAAGCCTATGACAAAGGAAGATATGCTCTTGTGGAGAAACCAATGCATCACTTCCATTGTTTGCTACATCACAATCTCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

422

Amino Acids

47.72

Weight (kDa)

6.4

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rieske PF00355 96 - 182 4.8e-20 Rieske [2Fe-2S] domain
Ring_hydroxyl_A PF00848 247 - 416 1.1e-28 Ring hydroxylating alpha subunit (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 966
AccII CGCG 1 cut(s) 959
AciI CCGC 2 cut(s) 218, 722
AclWI GGATC 5 cut(s) 179, 233, 511, 949, 1064
AcsI RAATTY 5 cut(s) 32, 86, 107, 541, 973
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 3 cut(s) 233, 682, 810
AfiI CCNNNNNNNGG 2 cut(s) 630, 693
AflIII ACRYGT 1 cut(s) 957
AgsI TTSAA 3 cut(s) 73, 99, 909
AjiI CACGTC 1 cut(s) 743
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 7 cut(s) 169, 225, 262, 495, 518, 950, 1094
AluI AGCT 7 cut(s) 169, 225, 262, 495, 518, 950, 1094
Alw21I GWGCWC 2 cut(s) 227, 264
Alw26I GTCTC 2 cut(s) 641, 1178
AlwI GGATC 5 cut(s) 179, 233, 511, 949, 1064
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 5 cut(s) 32, 86, 107, 541, 973
Asp700I GAANNNNTTC 1 cut(s) 773
AspS9I GGNCC 3 cut(s) 593, 995, 1031
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 3 cut(s) 328, 410, 625
AsuII TTCGAA 1 cut(s) 873
AvaII GGWCC 3 cut(s) 593, 995, 1031
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BanII GRGCYC 2 cut(s) 227, 264
BauI CACGAG 2 cut(s) 170, 226
BbsI GAAGAC 4 cut(s) 136, 265, 927, 1045
Bbv12I GWGCWC 2 cut(s) 227, 264
BbvI GCAGC 1 cut(s) 632
BccI CCATC 5 cut(s) 279, 477, 788, 997, 1129
BceAI ACGGC 3 cut(s) 33, 134, 278
BcgI CGANNNNNNTGC 2 cut(s) 609, 643
BciVI GTATCC 2 cut(s) 528, 896
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 641, 1178
BfaI CTAG 2 cut(s) 522, 1016
BfmI CTRYAG 2 cut(s) 250, 618
BfuI GTATCC 2 cut(s) 528, 896
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 3 cut(s) 593, 995, 1031
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 3 cut(s) 593, 995, 1031
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 3 cut(s) 218, 674, 1237
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 4 cut(s) 136, 265, 927, 1045
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 3 cut(s) 517, 593, 1167
BpuMI CCSGG 1 cut(s) 340
BsaI GGTCTC 2 cut(s) 641, 1178
BsaJI CCNNGG 4 cut(s) 687, 703, 998, 1034
Bsc4I CCNNNNNNNGG 2 cut(s) 630, 693
BseDI CCNNGG 4 cut(s) 687, 703, 998, 1034
BseGI GGATG 4 cut(s) 290, 488, 1008, 1090
BseLI CCNNNNNNNGG 2 cut(s) 630, 693
BseXI GCAGC 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 959
Bsh1285I CGRYCG 1 cut(s) 268
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 2 cut(s) 227, 264
BsiSI CCGG 2 cut(s) 29, 339
BslFI GGGAC 3 cut(s) 576, 606, 852
BslI CCNNNNNNNGG 2 cut(s) 630, 693
BsmAI GTCTC 2 cut(s) 641, 1178
BsmFI GGGAC 3 cut(s) 576, 606, 852
Bso31I GGTCTC 2 cut(s) 641, 1178
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 227, 264
Bsp143I GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
BspACI CCGC 2 cut(s) 218, 722
BspFNI CGCG 1 cut(s) 959
BspHI TCATGA 1 cut(s) 978
BspLI GGNNCC 1 cut(s) 594
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 5 cut(s) 179, 233, 511, 949, 1064
BspT104I TTCGAA 1 cut(s) 873
BspTNI GGTCTC 2 cut(s) 641, 1178
BssECI CCNNGG 4 cut(s) 687, 703, 998, 1034
BssMI GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
BssSI CACGAG 2 cut(s) 170, 226
BssT1I CCWWGG 3 cut(s) 703, 998, 1034
Bst2BI CACGAG 2 cut(s) 170, 226
Bst4CI ACNGT 4 cut(s) 25, 254, 926, 1266
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 3 cut(s) 575, 725, 790
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 4 cut(s) 290, 488, 1008, 1090
BstFNI CGCG 1 cut(s) 959
BstKTI GATC 9 cut(s) 187, 241, 268, 310, 432, 506, 847, 944, 1072
BstMAI GTCTC 2 cut(s) 641, 1178
BstMBI GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 1 cut(s) 956
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 338
BstSFI CTRYAG 2 cut(s) 250, 618
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 1 cut(s) 632
BstV2I GAAGAC 4 cut(s) 136, 265, 927, 1045
BstX2I RGATCY 1 cut(s) 941
BstXI CCANNNNNNTGG 1 cut(s) 896
BstYI RGATCY 1 cut(s) 941
BsuI GTATCC 2 cut(s) 528, 896
BtgI CCRYGG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 4 cut(s) 290, 488, 1008, 1090
BtsIMutI CAGTG 2 cut(s) 30, 234
Cac8I GCNNGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 978
Cfr13I GGNCC 3 cut(s) 593, 995, 1031
Csp6I GTAC 3 cut(s) 232, 681, 809
CviAII CATG 5 cut(s) 395, 422, 479, 806, 979
CviQI GTAC 3 cut(s) 232, 681, 809
DdeI CTNAG 3 cut(s) 575, 725, 790
DpnI GATC 9 cut(s) 186, 240, 267, 309, 431, 505, 846, 943, 1071
DpnII GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
DraI TTTAAA 1 cut(s) 1080
EciI GGCGGA 1 cut(s) 207
Ecl136II GAGCTC 2 cut(s) 225, 262
Eco130I CCWWGG 3 cut(s) 703, 998, 1034
Eco24I GRGCYC 2 cut(s) 227, 264
Eco31I GGTCTC 2 cut(s) 641, 1178
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 3 cut(s) 593, 995, 1031
Eco53kI GAGCTC 2 cut(s) 225, 262
Eco57I CTGAAG 1 cut(s) 966
EcoICRI GAGCTC 2 cut(s) 225, 262
EcoO109I RGGNCCY 1 cut(s) 593
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 3 cut(s) 703, 998, 1034
EcoT22I ATGCAT 1 cut(s) 1230
EcoT38I GRGCYC 2 cut(s) 227, 264
ErhI CCWWGG 3 cut(s) 703, 998, 1034
FaeI CATG 5 cut(s) 398, 425, 482, 809, 982
FaqI GGGAC 3 cut(s) 576, 606, 852
FatI CATG 5 cut(s) 394, 421, 478, 805, 978
FauI CCCGC 1 cut(s) 729
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 4 cut(s) 297, 495, 1015, 1097
FriOI GRGCYC 2 cut(s) 227, 264
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 2 cut(s) 522, 1016
GluI GCNGC 1 cut(s) 621
HapII CCGG 2 cut(s) 29, 339
Hin1II CATG 5 cut(s) 398, 425, 482, 809, 982
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 1092
HinfI GANTC 5 cut(s) 141, 193, 322, 716, 1178
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 2 cut(s) 29, 339
HphI GGTGA 3 cut(s) 328, 410, 625
Hpy166II GTNNAC 3 cut(s) 15, 234, 967
Hpy188III TCNNGA 9 cut(s) 172, 326, 441, 837, 848, 979, 1067, 1102, 1175
Hpy8I GTNNAC 3 cut(s) 15, 234, 967
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 628, 763, 1083
HpyCH4III ACNGT 4 cut(s) 25, 254, 926, 1266
HpyCH4IV ACGT 2 cut(s) 11, 742
HpyCH4V TGCA 4 cut(s) 620, 738, 818, 1228
HpyF10VI GCNNNNNNNGC 1 cut(s) 956
HpyF3I CTNAG 3 cut(s) 575, 725, 790
HpySE526I ACGT 2 cut(s) 11, 742
Hsp92II CATG 5 cut(s) 398, 425, 482, 809, 982
Ksp22I TGATCA 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
LpnPI CCDG 5 cut(s) 42, 73, 151, 352, 426
Lsp1109I GCAGC 1 cut(s) 632
LweI GCATC 3 cut(s) 218, 674, 1237
MaeI CTAG 2 cut(s) 522, 1016
MaeII ACGT 2 cut(s) 11, 742
MaeIII GTNAC 3 cut(s) 172, 326, 613
MalI GATC 9 cut(s) 186, 240, 267, 309, 431, 505, 846, 943, 1071
MboI GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
MboII GAAGA 9 cut(s) 132, 136, 202, 265, 932, 1045, 1127, 1151, 1212
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 2 cut(s) 227, 264
MluCI AATT 9 cut(s) 32, 73, 86, 107, 245, 541, 656, 763, 973
MluI ACGCGT 1 cut(s) 957
MlyI GAGTC 2 cut(s) 150, 331
MmeI TCCRAC 2 cut(s) 95, 158
Mph1103I ATGCAT 1 cut(s) 1230
MroXI GAANNNNTTC 1 cut(s) 773
MseI TTAA 4 cut(s) 14, 315, 551, 1079
MslI CAYNNNNRTG 1 cut(s) 670
MspI CCGG 2 cut(s) 29, 339
MspR9I CCNGG 1 cut(s) 340
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 1 cut(s) 956
NciI CCSGG 1 cut(s) 340
NdeII GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
NlaIII CATG 5 cut(s) 398, 425, 482, 809, 982
NlaIV GGNNCC 1 cut(s) 594
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 172, 326, 613
NsiI ATGCAT 1 cut(s) 1230
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 1 cut(s) 873
PagI TCATGA 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 773
PfeI GAWTC 3 cut(s) 193, 716, 1178
PkrI GCNGC 1 cut(s) 622
Ple19I CGATCG 1 cut(s) 268
PleI GAGTC 2 cut(s) 149, 330
PpsI GAGTC 2 cut(s) 149, 330
PpuMI RGGWCCY 1 cut(s) 593
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 227, 264
Psp5II RGGWCCY 1 cut(s) 593
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 3 cut(s) 593, 995, 1031
PspPPI RGGWCCY 1 cut(s) 593
PstI CTGCAG 1 cut(s) 622
PsuI RGATCY 1 cut(s) 941
PvuI CGATCG 1 cut(s) 268
RsaI GTAC 3 cut(s) 233, 682, 810
RsaNI GTAC 3 cut(s) 232, 681, 809
RseI CAYNNNNRTG 1 cut(s) 670
SacI GAGCTC 2 cut(s) 227, 264
SaqAI TTAA 4 cut(s) 14, 315, 551, 1079
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 9 cut(s) 184, 238, 265, 307, 429, 503, 844, 941, 1069
Sau96I GGNCC 3 cut(s) 593, 995, 1031
SchI GAGTC 2 cut(s) 150, 331
ScrFI CCNGG 1 cut(s) 340
SduI GDGCHC 2 cut(s) 227, 264
SfaNI GCATC 3 cut(s) 218, 674, 1237
SfcI CTRYAG 2 cut(s) 250, 618
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 3 cut(s) 593, 995, 1031
SmiMI CAYNNNNRTG 1 cut(s) 670
SmlI CTYRAG 3 cut(s) 496, 608, 1182
SmoI CTYRAG 3 cut(s) 496, 608, 1182
Sse9I AATT 9 cut(s) 32, 73, 86, 107, 245, 541, 656, 763, 973
SsiI CCGC 2 cut(s) 218, 722
SspMI CTAG 2 cut(s) 522, 1016
SstI GAGCTC 2 cut(s) 227, 264
StyD4I CCNGG 1 cut(s) 338
StyI CCWWGG 3 cut(s) 703, 998, 1034
TaaI ACNGT 4 cut(s) 25, 254, 926, 1266
TaiI ACGT 2 cut(s) 14, 745
TaqI TCGA 8 cut(s) 258, 264, 428, 629, 849, 873, 1056, 1176
TaqII GACCGA 1 cut(s) 665
TasI AATT 9 cut(s) 32, 73, 86, 107, 245, 541, 656, 763, 973
TfiI GAWTC 3 cut(s) 193, 716, 1178
Tru1I TTAA 4 cut(s) 14, 315, 551, 1079
Tru9I TTAA 4 cut(s) 14, 315, 551, 1079
TscAI CASTG 2 cut(s) 30, 241
TseFI GTSAC 3 cut(s) 172, 326, 613
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 3 cut(s) 172, 326, 613
TspDTI ATGAA 4 cut(s) 669, 967, 1087, 1104
TspGWI ACGGA 2 cut(s) 191, 676
TspRI CASTG 2 cut(s) 30, 241
VpaK11BI GGWCC 3 cut(s) 593, 995, 1031
XapI RAATTY 5 cut(s) 32, 86, 107, 541, 973
XceI RCATGY 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 773
XspI CTAG 2 cut(s) 522, 1016
Zsp2I ATGCAT 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.