Prupe.1G072500_v2.0.a1

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005808
Physical Location & Seq
Forward (+)
5167649 .. 5170950
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G072500.3

Sequence Viewer

Length: 1245 bp
ATGGAGATGGCGATGACTGTGATGAATGCGGCAACTCTCCAACCCAAACCTCTTCGACCCAAAGGCCCTTACCTTCTTCCAAACCCAAGAAGAGTCATCAACTTCAAGGCCTCTTCTCTCCACAACCCTCAAGTCTCATCAATCGGAGCTCCAACTCTGGTGGGTCAATTCGACCCAACAATCCCCATAGAGCGAGCCGTAACTCCACCCAGCTCATGGTACACCGATCCTTCCTTTTACTCTCTCGAACTCGACACCCTCTTCTACCGTGGATGGCAGGCCGCAGGATACACTGAACAGATAAAAAATCCAGGGGAGTTTTTCACTTGCAGATTGGGAAACGCAGAGTTTGTTGTGTGCCGAGATGGTGATGGCAAGCTTCAAGCTTTCCACAATGTATGTCGACATCATGCCTCACTTCTTGCATATGGAAGCGGCCGCAAGTCTTGCTTTGTGTGCCCTTATCATGGATGGACTTATGGGTTAGACGGCTCACTTCTCAAGGCAACTAGAATAACTGGAATACAAAATTTCAATGAACATGAGTTTGGGCTTATATCGGTGAAAGCAGCTACTTGGGGCCCGTTTGTTCTTCTCAATATGGAGCAGGATGTTGATAGTGATACAGACATAGTGGAAAAGGAATGGCTTGGCAGCTCTTCAGAACTACTGAGTAACAACGGGGTCGATTCCTCATTAAGTTTTGTTTGTAGACGTGATTATATGATTGAATGTAACTGGAAGGTCTTTTGTGACAACTACTTAGATGGCGGATATCATGTTCCTTATGCGCATAAAGGCCTTGCATCTGGTCTTAATCTTGATCATTACTCTACCACAATATATGAAAAGGTCAGCATTCAAAAGTGTGAAGGTGGCTCGACAGAAAGAACAAATGATTATGATCGGCTTGGATCAAAAGCTCTATATGCTTTTGTTTATCCTAATTTCATGATTAATAGGTATGGACCATGGATGGACACCAATCTAGTACTGCCACTTGGACCAAGGAAATGTCAAGTGATATTTGATTACTTCATTGAACCTTCTCTTATGGATAACAAAGATTTTATAGAGAGAAGTTTGAAAGACAGTGAAAGAGTACAGGTGGAAGATGTCGTGCTATGTGAAGGTGTCCAAAGAGGTCTTGAATCACCTGCATATGATATCGGCAGATACGCTCCGACTGTCGAGAATGCCATGCACCATTTCCATTGTCTGCTTCACAAAAGCCTGAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

415

Amino Acids

46.77

Weight (kDa)

6.01

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1165
Acc16I TGCGCA 1 cut(s) 792
Acc36I ACCTGC 1 cut(s) 1165
AccB7I CCANNNNNTGG 1 cut(s) 216
AccI GTMKAC 2 cut(s) 403, 712
AciI CCGC 5 cut(s) 29, 282, 435, 439, 771
AclWI GGATC 2 cut(s) 221, 922
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 3 cut(s) 221, 993, 1104
AfiI CCNNNNNNNGG 2 cut(s) 216, 467
AgsI TTSAA 8 cut(s) 106, 383, 535, 731, 863, 1043, 1087, 1151
AjiI CACGTC 1 cut(s) 716
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AloI GAACNNNNNNTCC 2 cut(s) 765, 797
AluBI AGCT 7 cut(s) 149, 213, 379, 386, 572, 657, 923
AluI AGCT 7 cut(s) 149, 213, 379, 386, 572, 657, 923
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 2 cut(s) 221, 922
AoxI GGCC 6 cut(s) 64, 108, 279, 436, 580, 799
ApaI GGGCCC 1 cut(s) 584
ApeKI GCWGC 2 cut(s) 569, 654
ApoI RAATTY 1 cut(s) 529
AseI ATTAAT 1 cut(s) 957
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 5 cut(s) 65, 580, 581, 968, 1004
AsuHPI GGTGA 3 cut(s) 380, 574, 1146
AvaII GGWCC 2 cut(s) 968, 1004
BaeGI GKGCMC 2 cut(s) 461, 584
BanII GRGCYC 2 cut(s) 151, 584
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 581, 666
BccI CCATC 6 cut(s) 267, 359, 365, 465, 761, 970
BceAI ACGGC 2 cut(s) 182, 505
BciT130I CCWGG 1 cut(s) 312
BciVI GTATCC 1 cut(s) 281
BclI TGATCA 1 cut(s) 823
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 2 cut(s) 510, 989
BfuAI ACCTGC 1 cut(s) 1165
BfuI GTATCC 1 cut(s) 281
BisI GCNGC 6 cut(s) 30, 282, 436, 439, 570, 655
BlsI GCNGC 6 cut(s) 31, 283, 437, 440, 571, 656
BmcAI AGTACT 1 cut(s) 993
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 2 cut(s) 968, 1004
BmgBI CACGTC 1 cut(s) 716
BmgT120I GGNCC 5 cut(s) 65, 580, 581, 968, 1004
BmiI GGNNCC 2 cut(s) 581, 582
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 1 cut(s) 815
BpuEI CTTGAG 2 cut(s) 114, 485
BsaBI GATNNNNATC 1 cut(s) 903
BsaJI CCNNGG 4 cut(s) 268, 311, 971, 1007
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 467
Bse1I ACTGG 2 cut(s) 523, 743
Bse8I GATNNNNATC 1 cut(s) 903
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 4 cut(s) 268, 311, 971, 1007
BseGI GGATG 4 cut(s) 278, 476, 616, 981
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 2 cut(s) 216, 467
BseMII CTCAG 1 cut(s) 662
BseNI ACTGG 2 cut(s) 523, 743
BseSI GKGCMC 2 cut(s) 461, 584
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 2 cut(s) 581, 666
BseYI CCCAGC 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 6 cut(s) 66, 110, 281, 438, 582, 801
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 216, 467
BsmAI GTCTC 1 cut(s) 139
BsmI GAATGC 3 cut(s) 31, 858, 1201
BsnI GGCC 6 cut(s) 66, 110, 281, 438, 582, 801
Bsp120I GGGCCC 1 cut(s) 580
Bsp1286I GDGCHC 3 cut(s) 151, 461, 584
Bsp143I GATC 4 cut(s) 226, 823, 904, 914
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 5 cut(s) 29, 282, 435, 439, 771
BspANI GGCC 6 cut(s) 66, 110, 281, 438, 582, 801
BspCNI CTCAG 1 cut(s) 663
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 2 cut(s) 581, 582
BspMI ACCTGC 1 cut(s) 1165
BspPI GGATC 2 cut(s) 221, 922
BspQI GCTCTTC 1 cut(s) 664
BsrI ACTGG 2 cut(s) 523, 743
BssECI CCNNGG 4 cut(s) 268, 311, 971, 1007
BssMI GATC 4 cut(s) 226, 823, 904, 914
BssT1I CCWWGG 2 cut(s) 971, 1007
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 4 cut(s) 19, 269, 1094, 1189
Bst6I CTCTTC 5 cut(s) 57, 85, 118, 266, 664
BstAPI GCANNNNNTGC 1 cut(s) 447
BstC8I GCNNGC 3 cut(s) 195, 279, 377
BstDEI CTNAG 2 cut(s) 671, 763
BstDSI CCRYGG 2 cut(s) 268, 971
BstF5I GGATG 4 cut(s) 278, 476, 616, 981
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 4 cut(s) 229, 826, 907, 917
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 4 cut(s) 226, 823, 904, 914
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 447, 456, 929
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 310
BstSLI GKGCMC 2 cut(s) 461, 584
BstV1I GCAGC 2 cut(s) 581, 666
BstZI CGGCCG 1 cut(s) 436
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 6 cut(s) 66, 110, 281, 438, 582, 801
BtgI CCRYGG 2 cut(s) 268, 971
BtgZI GCGATG 1 cut(s) 26
BtrI CACGTC 1 cut(s) 716
BtsCI GGATG 4 cut(s) 278, 476, 616, 981
BtsIMutI CAGTG 2 cut(s) 291, 1099
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 3 cut(s) 195, 279, 377
CciI TCATGA 1 cut(s) 951
CciNI GCGGCCGC 1 cut(s) 436
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 5 cut(s) 65, 580, 581, 968, 1004
Csp6I GTAC 3 cut(s) 220, 992, 1103
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 8 cut(s) 216, 410, 467, 542, 779, 952, 972, 1201
CviQI GTAC 3 cut(s) 220, 992, 1103
DdeI CTNAG 2 cut(s) 671, 763
DpnI GATC 4 cut(s) 228, 825, 906, 916
DpnII GATC 4 cut(s) 226, 823, 904, 914
EaeI YGGCCR 1 cut(s) 436
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 5 cut(s) 57, 85, 118, 266, 664
EarI CTCTTC 5 cut(s) 57, 85, 118, 266, 664
EciI GGCGGA 1 cut(s) 786
Ecl136II GAGCTC 1 cut(s) 149
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 971, 1007
Eco147I AGGCCT 2 cut(s) 110, 801
Eco24I GRGCYC 2 cut(s) 151, 584
Eco32I GATATC 2 cut(s) 776, 1168
Eco47I GGWCC 2 cut(s) 968, 1004
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 149
EcoO109I RGGNCCY 2 cut(s) 65, 580
EcoRII CCWGG 1 cut(s) 310
EcoRV GATATC 2 cut(s) 776, 1168
EcoT14I CCWWGG 2 cut(s) 971, 1007
EcoT38I GRGCYC 2 cut(s) 151, 584
ErhI CCWWGG 2 cut(s) 971, 1007
FaeI CATG 8 cut(s) 219, 413, 470, 545, 782, 955, 975, 1204
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FatI CATG 8 cut(s) 215, 409, 466, 541, 778, 951, 971, 1200
FauNDI CATATG 2 cut(s) 427, 1162
FbaI TGATCA 1 cut(s) 823
FblI GTMKAC 2 cut(s) 403, 712
Fnu4HI GCNGC 6 cut(s) 30, 282, 436, 439, 570, 655
FokI GGATG 4 cut(s) 285, 483, 623, 988
FriOI GRGCYC 2 cut(s) 151, 584
Fsp4HI GCNGC 6 cut(s) 30, 282, 436, 439, 570, 655
FspAI RTGCGCAY 1 cut(s) 792
FspBI CTAG 2 cut(s) 510, 989
FspI TGCGCA 1 cut(s) 792
GlaI GCGC 1 cut(s) 792
GluI GCNGC 6 cut(s) 30, 282, 436, 439, 570, 655
GsaI CCCAGC 1 cut(s) 213
HaeIII GGCC 6 cut(s) 66, 110, 281, 438, 582, 801
HhaI GCGC 1 cut(s) 793
Hin1II CATG 8 cut(s) 219, 413, 470, 545, 782, 955, 975, 1204
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 404
HindII GTYRAC 1 cut(s) 404
HindIII AAGCTT 2 cut(s) 377, 384
HinfI GANTC 3 cut(s) 93, 689, 1151
HphI GGTGA 3 cut(s) 380, 574, 1146
Hpy166II GTNNAC 3 cut(s) 222, 404, 713
Hpy188I TCNGA 3 cut(s) 146, 664, 1185
Hpy188III TCNNGA 5 cut(s) 245, 821, 952, 1148, 1192
Hpy8I GTNNAC 3 cut(s) 222, 404, 713
HpyAV CCTTC 6 cut(s) 83, 240, 736, 866, 1056, 1124
HpyCH4III ACNGT 4 cut(s) 19, 269, 1094, 1189
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 5 cut(s) 330, 425, 806, 1160, 1204
HpyF10VI GCNNNNNNNGC 3 cut(s) 447, 456, 929
HpyF3I CTNAG 2 cut(s) 671, 763
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 8 cut(s) 219, 413, 470, 545, 782, 955, 975, 1204
HspAI GCGC 1 cut(s) 791
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 4 cut(s) 226, 823, 904, 914
LguI GCTCTTC 1 cut(s) 664
LmnI GCTCC 4 cut(s) 146, 154, 604, 1186
Lsp1109I GCAGC 2 cut(s) 581, 666
LweI GCATC 1 cut(s) 815
MaeI CTAG 2 cut(s) 510, 989
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 4 cut(s) 199, 674, 734, 752
MalI GATC 4 cut(s) 228, 825, 906, 916
MboI GATC 4 cut(s) 226, 823, 904, 914
MboII GAAGA 8 cut(s) 44, 68, 102, 105, 253, 584, 651, 1124
MhlI GDGCHC 3 cut(s) 151, 461, 584
MluCI AATT 3 cut(s) 167, 529, 946
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 3 cut(s) 64, 176, 1208
MnlI CCTC 7 cut(s) 60, 121, 138, 269, 424, 703, 1136
MseI TTAA 3 cut(s) 698, 816, 957
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 3 cut(s) 31, 858, 1201
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 447, 456, 929
NcoI CCATGG 1 cut(s) 971
NdeI CATATG 2 cut(s) 427, 1162
NdeII GATC 4 cut(s) 226, 823, 904, 914
NlaIII CATG 8 cut(s) 219, 413, 470, 545, 782, 955, 975, 1204
NlaIV GGNNCC 2 cut(s) 581, 582
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 1 cut(s) 752
NotI GCGGCCGC 1 cut(s) 436
NsbI TGCGCA 1 cut(s) 792
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 1165
PceI AGGCCT 2 cut(s) 110, 801
PciSI GCTCTTC 1 cut(s) 664
PctI GAATGC 3 cut(s) 31, 858, 1201
PfeI GAWTC 2 cut(s) 689, 1151
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 6 cut(s) 31, 283, 437, 440, 571, 656
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PshBI ATTAAT 1 cut(s) 957
Psp124BI GAGCTC 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 581, 582
PspOMI GGGCCC 1 cut(s) 580
PspPI GGNCC 5 cut(s) 65, 580, 581, 968, 1004
RsaI GTAC 3 cut(s) 221, 993, 1104
RsaNI GTAC 3 cut(s) 220, 992, 1103
SacI GAGCTC 1 cut(s) 151
SalI GTCGAC 1 cut(s) 402
SapI GCTCTTC 1 cut(s) 664
SaqAI TTAA 3 cut(s) 698, 816, 957
SatI GCNGC 6 cut(s) 30, 282, 436, 439, 570, 655
Sau3AI GATC 4 cut(s) 226, 823, 904, 914
Sau96I GGNCC 5 cut(s) 65, 580, 581, 968, 1004
ScaI AGTACT 1 cut(s) 993
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 3 cut(s) 151, 461, 584
SfaNI GCATC 1 cut(s) 815
SinI GGWCC 2 cut(s) 968, 1004
SmlI CTYRAG 2 cut(s) 129, 500
SmoI CTYRAG 2 cut(s) 129, 500
Sse9I AATT 3 cut(s) 167, 529, 946
SseBI AGGCCT 2 cut(s) 110, 801
SsiI CCGC 5 cut(s) 29, 282, 435, 439, 771
SspMI CTAG 2 cut(s) 510, 989
SstI GAGCTC 1 cut(s) 151
StuI AGGCCT 2 cut(s) 110, 801
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 2 cut(s) 971, 1007
TaaI ACNGT 4 cut(s) 19, 269, 1094, 1189
TaiI ACGT 1 cut(s) 718
TaqI TCGA 8 cut(s) 55, 171, 246, 252, 403, 687, 881, 1191
TasI AATT 3 cut(s) 167, 529, 946
TatI WGTACW 2 cut(s) 991, 1102
TauI GCSGC 4 cut(s) 32, 284, 438, 441
TfiI GAWTC 2 cut(s) 689, 1151
Tru1I TTAA 3 cut(s) 698, 816, 957
Tru9I TTAA 3 cut(s) 698, 816, 957
TscAI CASTG 2 cut(s) 298, 1099
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 2 cut(s) 569, 654
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 5 cut(s) 38, 552, 861, 940, 1027
TspRI CASTG 2 cut(s) 298, 1099
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 2 cut(s) 968, 1004
VspI ATTAAT 1 cut(s) 957
XapI RAATTY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmiI GTMKAC 2 cut(s) 403, 712
XspI CTAG 2 cut(s) 510, 989
ZrmI AGTACT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.