Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005808

Physical Location & Seq

Genomic Coordinates Reverse (-)

25021398 .. 25024444

Gene Structure 3047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1241100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGCGATGACTGTGGCAACTTTCCAACCCAAAACACTTCGACCCAAAAGCCCTTACCTTCTTCCCAATCTACCCAGAAGAGTCTGCAACTTCAAGGCCTTCTCCCTCCACAATCCCCAGCTCCCATCACTCGTGGGTCAGTTCGAACCCACCATCCCAATAGAGAGAGCAGGGACTCCTCCCAGCTCATGGTACACTAATCCATCATTTTACTCTCTCGAAATCAACACCCTTTTCTACCGTGGATGGCAGGCTGTAGGATATACAGAACAGATTAGTAATGCAGGGGAGTTTTTCACTGGCAGATTGGGAAACGTAGAGTTTGTGGTATGCCAAGATGGTGATGGAAAGCTTCAAGCTTTTCACAATGTCTGTCGACATCACGCGATGCTCCTTGCATATGGAAGCGGCCGCAAGTCCTGCTTTGTCTGCCCTTATCATGGATGGACGTATGGGTTAGATGGTGCACTTCTCAAGGCAACTAGGATAACTGGAATACAGAATTTCAATGAAAACGAGTTTGGACTTGTACCACTAAAAGTAGCAACTTGGGGACCGTTTATTCTTCTCAATATGGAACAGAATGTTGACAGTGATCCAGGCAGTGTGCAAAATGAATGGCTTGGCAGCTCCGCAGAAGTACTGAGTAACAACGGGATTGATACTTCATTAAGTTTTGTTTGCCGACGTGATTATGTGATTGAATGTAACTGGAAGGTCTTTTGTGACAACTACTTAGATGGGGGGTACCACGTGCCTTATGCACATAAAGGCCTTGCATCTGGTCTTAATCTTGACGGTTACTCTACCACAATGTATGAAAAGGTCAGCATTCAAAAGTGTGAAAACGGCTCGACAGAAAGAAAAAATGATTTTGATCGGCTTGGGTCAAAAGCGCTGTATGCATTCGTGTATCCAAATTTCATGATTAATAGGTATGGACCTTGGATGGATACCAATCTGGTATTGCCGCTCGGACCGCGGAAATGTCAAGTGAGATTTGATTACTTTATTGAAGCTTCTCTTAAGGATGACAGAGATTTTATAGAGAGAAGTTTGAAAGATAGTGAAAGAGTACAGACGGAAGATGTCATGCTATGTGAAGGCGTTCAAAGAGGTTTGGAATCGCCCGCGTACAATATCGGTAGATACGCCCCGACTGTTGAGAATGCCATGCACCATTTCCATTGCCTGCTTCACAGGAGCTTAACAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00260 map00260

Pfam Domains

Protein Families

Rieske Ring_hydroxyl_A

Protein Analysis

406

Amino Acids

45.9

Weight (kDa)

6.27

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rieske	PF00355	83 - 169	8.9e-18	Rieske [2Fe-2S] domain
Ring_hydroxyl_A	PF00848	228 - 403	1.9e-31	Ring hydroxylating alpha subunit (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29890
fragaria_vesca	FvH4_4g06930 FvH4_4g06930 FvH4_4g06930 FvH4_4g06930 FvH4_4g06930
malus_domestica	MD13G1241100.v1.1
prunus_persica	Prupe.1G072500_v2.0.a1 Prupe.1G072500_v2.0.a1 Prupe.1G072500_v2.0.a1 Prupe.1G072500_v2.0.a1
pyrus_communis	pycom13g21200
rosa_chinensis	RchiOBHm_Chr4g0398871
rosa_laevigata	RLG00000009310 RLG00000009315
rosa_multiflora	Rmu_ssc0000064.1_g000028
rosa_roxburghii	Rroxscaffold_5G00343910 Rroxscaffold_7G00183470
rosa_rugosa	Rorug04G0012400 Rorug04G0012800 Rorug04G0012900 Rorug04G0013000 Rorug04G0013100
rosa_samantha	Rh4AG090500 Rh4BG087800 Rh4CG098800 Rh4DG082800
rosa_wichuraiana	Rw4G007490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	747
AccB1I	GGYRCC	1 cut(s)	747
AccB7I	CCANNNNNTGG	1 cut(s)	189
AccBSI	CCGCTC	1 cut(s)	973
AccI	GTMKAC	1 cut(s)	376
AccII	CGCG	3 cut(s)	386, 982, 1133
AciI	CCGC	7 cut(s)	408, 412, 633, 971, 980, 982, 1131
AclWI	GGATC	1 cut(s)	590
AcoI	YGGCCR	1 cut(s)	409
AcsI	RAATTY	2 cut(s)	502, 919
AcvI	CACGTG	1 cut(s)	754
AfaI	GTAC	6 cut(s)	194, 531, 642, 749, 1077, 1136
AfeI	AGCGCT	1 cut(s)	897
AfiI	CCNNNNNNNGG	2 cut(s)	189, 440
AflII	CTTAAG	1 cut(s)	1025
AgsI	TTSAA	8 cut(s)	94, 356, 508, 704, 836, 1016, 1060, 1112
AjiI	CACGTC	1 cut(s)	689
AjnI	CCWGG	1 cut(s)	598
AjuI	GAANNNNNNNTTGG	2 cut(s)	504, 536
AloI	GAACNNNNNNTCC	2 cut(s)	138, 170
AluBI	AGCT	7 cut(s)	121, 186, 352, 359, 630, 1019, 1206
AluI	AGCT	7 cut(s)	121, 186, 352, 359, 630, 1019, 1206
Alw21I	GWGCWC	1 cut(s)	469
Alw44I	GTGCAC	1 cut(s)	465
AlwI	GGATC	1 cut(s)	590
Aor51HI	AGCGCT	1 cut(s)	897
AoxI	GGCC	3 cut(s)	96, 409, 772
ApaLI	GTGCAC	1 cut(s)	465
ApeKI	GCWGC	1 cut(s)	627
ApoI	RAATTY	2 cut(s)	502, 919
AseI	ATTAAT	1 cut(s)	930
Asp700I	GAANNNNTTC	1 cut(s)	1107
Asp718I	GGTACC	1 cut(s)	747
AspLEI	GCGC	1 cut(s)	898
AspS9I	GGNCC	3 cut(s)	554, 941, 977
AsuHPI	GGTGA	1 cut(s)	353
AsuII	TTCGAA	1 cut(s)	144
AvaII	GGWCC	3 cut(s)	554, 941, 977
BaeGI	GKGCMC	1 cut(s)	469
BanI	GGYRCC	1 cut(s)	747
BauI	CACGAG	1 cut(s)	131
BbrPI	CACGTG	1 cut(s)	754
Bbv12I	GWGCWC	1 cut(s)	469
BbvI	GCAGC	1 cut(s)	639
BceAI	ACGGC	1 cut(s)	865
BciT130I	CCWGG	1 cut(s)	600
BciVI	GTATCC	2 cut(s)	924, 946
BfaI	CTAG	1 cut(s)	483
BfmI	CTRYAG	1 cut(s)	255
BfoI	RGCGCY	1 cut(s)	899
BfrI	CTTAAG	1 cut(s)	1025
BfuI	GTATCC	2 cut(s)	924, 946
BisI	GCNGC	4 cut(s)	409, 412, 628, 971
BlsI	GCNGC	4 cut(s)	410, 413, 629, 972
BmcAI	AGTACT	1 cut(s)	642
Bme1390I	CCNGG	1 cut(s)	600
Bme18I	GGWCC	3 cut(s)	554, 941, 977
BmgBI	CACGTC	1 cut(s)	689
BmgT120I	GGNCC	3 cut(s)	554, 941, 977
BmiI	GGNNCC	2 cut(s)	555, 749
BmrFI	CCNGG	1 cut(s)	600
BmsI	GCATC	2 cut(s)	378, 788
Bpu14I	TTCGAA	1 cut(s)	144
BpuEI	CTTGAG	1 cut(s)	458
BsaAI	YACGTR	1 cut(s)	754
BsaBI	GATNNNNATC	2 cut(s)	876, 957
BsaJI	CCNNGG	3 cut(s)	241, 944, 980
Bsc4I	CCNNNNNNNGG	2 cut(s)	189, 440
Bse1I	ACTGG	3 cut(s)	304, 496, 716
Bse3DI	GCAATG	1 cut(s)	1186
Bse8I	GATNNNNATC	2 cut(s)	876, 957
BseBI	CCWGG	1 cut(s)	600
BseDI	CCNNGG	3 cut(s)	241, 944, 980
BseGI	GGATG	5 cut(s)	153, 251, 449, 954, 1036
BseJI	GATNNNNATC	2 cut(s)	876, 957
BseLI	CCNNNNNNNGG	2 cut(s)	189, 440
BseMI	GCAATG	1 cut(s)	1186
BseMII	CTCAG	1 cut(s)	635
BseNI	ACTGG	3 cut(s)	304, 496, 716
BseRI	GAGGAG	1 cut(s)	168
BseSI	GKGCMC	1 cut(s)	469
BseX3I	CGGCCG	1 cut(s)	409
BseXI	GCAGC	1 cut(s)	639
BseYI	CCCAGC	2 cut(s)	117, 182
Bsh1236I	CGCG	3 cut(s)	386, 982, 1133
Bsh1285I	CGRYCG	1 cut(s)	412
BshFI	GGCC	3 cut(s)	98, 411, 774
BshNI	GGYRCC	1 cut(s)	747
BsiEI	CGRYCG	1 cut(s)	412
BsiHKAI	GWGCWC	1 cut(s)	469
BslFI	GGGAC	2 cut(s)	187, 567
BslI	CCNNNNNNNGG	2 cut(s)	189, 440
BsmFI	GGGAC	2 cut(s)	187, 567
BsmI	GAATGC	3 cut(s)	831, 905, 1174
BsnI	GGCC	3 cut(s)	98, 411, 774
Bsp119I	TTCGAA	1 cut(s)	144
Bsp1286I	GDGCHC	1 cut(s)	469
Bsp143I	GATC	2 cut(s)	595, 877
BspACI	CCGC	7 cut(s)	408, 412, 633, 971, 980, 982, 1131
BspANI	GGCC	3 cut(s)	98, 411, 774
BspCNI	CTCAG	1 cut(s)	636
BspFNI	CGCG	3 cut(s)	386, 982, 1133
BspHI	TCATGA	1 cut(s)	924
BspLI	GGNNCC	2 cut(s)	555, 749
BspPI	GGATC	1 cut(s)	590
BspT104I	TTCGAA	1 cut(s)	144
BspT107I	GGYRCC	1 cut(s)	747
BspTI	CTTAAG	1 cut(s)	1025
BsrBI	CCGCTC	1 cut(s)	973
BsrDI	GCAATG	1 cut(s)	1186
BsrI	ACTGG	3 cut(s)	304, 496, 716
BssECI	CCNNGG	3 cut(s)	241, 944, 980
BssMI	GATC	2 cut(s)	595, 877
BssSI	CACGAG	1 cut(s)	131
BssT1I	CCWWGG	1 cut(s)	944
Bst2BI	CACGAG	1 cut(s)	131
Bst2UI	CCWGG	1 cut(s)	600
Bst4CI	ACNGT	6 cut(s)	13, 242, 558, 593, 800, 1162
Bst6I	CTCTTC	1 cut(s)	73
BstAFI	CTTAAG	1 cut(s)	1025
BstAPI	GCANNNNNTGC	1 cut(s)	420
BstBAI	YACGTR	1 cut(s)	754
BstBI	TTCGAA	1 cut(s)	144
BstC8I	GCNNGC	3 cut(s)	252, 1131, 1193
BstDEI	CTNAG	2 cut(s)	644, 736
BstDSI	CCRYGG	2 cut(s)	241, 980
BstF5I	GGATG	5 cut(s)	153, 251, 449, 954, 1036
BstFNI	CGCG	3 cut(s)	386, 982, 1133
BstH2I	RGCGCY	1 cut(s)	899
BstHHI	GCGC	1 cut(s)	898
BstKTI	GATC	2 cut(s)	598, 880
BstMBI	GATC	2 cut(s)	595, 877
BstMCI	CGRYCG	1 cut(s)	412
BstMWI	GCNNNNNNNGC	4 cut(s)	420, 429, 902, 979
BstNI	CCWGG	1 cut(s)	600
BstSCI	CCNGG	1 cut(s)	598
BstSFI	CTRYAG	1 cut(s)	255
BstSLI	GKGCMC	1 cut(s)	469
BstUI	CGCG	3 cut(s)	386, 982, 1133
BstV1I	GCAGC	1 cut(s)	639
BstZI	CGGCCG	1 cut(s)	409
BsuI	GTATCC	2 cut(s)	924, 946
BsuRI	GGCC	3 cut(s)	98, 411, 774
BtgI	CCRYGG	2 cut(s)	241, 980
BtgZI	GCGATG	2 cut(s)	20, 401
BtrI	CACGTC	1 cut(s)	689
BtsCI	GGATG	5 cut(s)	153, 251, 449, 954, 1036
BtsI	GCAGTG	1 cut(s)	610
BtsIMutI	CAGTG	3 cut(s)	297, 598, 610
Cac8I	GCNNGC	3 cut(s)	252, 1131, 1193
CciI	TCATGA	1 cut(s)	924
CciNI	GCGGCCGC	1 cut(s)	409
CfoI	GCGC	1 cut(s)	898
Cfr13I	GGNCC	3 cut(s)	554, 941, 977
Cfr42I	CCGCGG	1 cut(s)	983
CpoI	CGGWCCG	1 cut(s)	977
Csp6I	GTAC	6 cut(s)	193, 530, 641, 748, 1076, 1135
CspI	CGGWCCG	1 cut(s)	977
CviAII	CATG	5 cut(s)	189, 440, 925, 1093, 1174
CviQI	GTAC	6 cut(s)	193, 530, 641, 748, 1076, 1135
DdeI	CTNAG	2 cut(s)	644, 736
DpnI	GATC	2 cut(s)	597, 879
DpnII	GATC	2 cut(s)	595, 877
EaeI	YGGCCR	1 cut(s)	409
EagI	CGGCCG	1 cut(s)	409
Eam1104I	CTCTTC	1 cut(s)	73
EarI	CTCTTC	1 cut(s)	73
EclXI	CGGCCG	1 cut(s)	409
Eco130I	CCWWGG	1 cut(s)	944
Eco147I	AGGCCT	2 cut(s)	98, 774
Eco47I	GGWCC	3 cut(s)	554, 941, 977
Eco47III	AGCGCT	1 cut(s)	897
Eco52I	CGGCCG	1 cut(s)	409
Eco72I	CACGTG	1 cut(s)	754
EcoRII	CCWGG	1 cut(s)	598
EcoT14I	CCWWGG	1 cut(s)	944
EcoT22I	ATGCAT	1 cut(s)	907
ErhI	CCWWGG	1 cut(s)	944
FaeI	CATG	5 cut(s)	192, 443, 928, 1096, 1177
FalI	AAGNNNNNCTT	4 cut(s)	407, 439, 1008, 1040
FaqI	GGGAC	2 cut(s)	187, 567
FatI	CATG	5 cut(s)	188, 439, 924, 1092, 1173
FauI	CCCGC	1 cut(s)	1138
FauNDI	CATATG	1 cut(s)	400
FblI	GTMKAC	1 cut(s)	376
Fnu4HI	GCNGC	4 cut(s)	409, 412, 628, 971
FokI	GGATG	5 cut(s)	140, 258, 456, 961, 1043
Fsp4HI	GCNGC	4 cut(s)	409, 412, 628, 971
FspBI	CTAG	1 cut(s)	483
GlaI	GCGC	1 cut(s)	897
GluI	GCNGC	4 cut(s)	409, 412, 628, 971
GsaI	CCCAGC	2 cut(s)	121, 186
HaeII	RGCGCY	1 cut(s)	899
HaeIII	GGCC	3 cut(s)	98, 411, 774
HhaI	GCGC	1 cut(s)	898
Hin1II	CATG	5 cut(s)	192, 443, 928, 1096, 1177
Hin6I	GCGC	1 cut(s)	896
HinP1I	GCGC	1 cut(s)	896
HincII	GTYRAC	2 cut(s)	377, 589
HindII	GTYRAC	2 cut(s)	377, 589
HindIII	AAGCTT	3 cut(s)	350, 357, 1017
HinfI	GANTC	3 cut(s)	81, 175, 1124
HphI	GGTGA	1 cut(s)	353
Hpy166II	GTNNAC	4 cut(s)	195, 377, 467, 589
Hpy188I	TCNGA	1 cut(s)	977
Hpy188III	TCNNGA	3 cut(s)	218, 794, 925
Hpy8I	GTNNAC	4 cut(s)	195, 377, 467, 589
Hpy99I	CGWCG	1 cut(s)	690
HpyAV	CCTTC	4 cut(s)	68, 109, 709, 1097
HpyCH4III	ACNGT	6 cut(s)	13, 242, 558, 593, 800, 1162
HpyCH4IV	ACGT	4 cut(s)	315, 449, 688, 753
HpyCH4V	TGCA	9 cut(s)	87, 284, 398, 467, 610, 764, 779, 905, 1177
HpyF10VI	GCNNNNNNNGC	4 cut(s)	420, 429, 902, 979
HpyF3I	CTNAG	2 cut(s)	644, 736
HpySE526I	ACGT	4 cut(s)	315, 449, 688, 753
Hsp92II	CATG	5 cut(s)	192, 443, 928, 1096, 1177
HspAI	GCGC	1 cut(s)	896
KpnI	GGTACC	1 cut(s)	751
KspI	CCGCGG	1 cut(s)	983
Kzo9I	GATC	2 cut(s)	595, 877
LmnI	GCTCC	4 cut(s)	126, 396, 635, 1203
Lsp1109I	GCAGC	1 cut(s)	639
LweI	GCATC	2 cut(s)	378, 788
MaeI	CTAG	1 cut(s)	483
MaeII	ACGT	4 cut(s)	315, 449, 688, 753
MaeIII	GTNAC	4 cut(s)	647, 707, 725, 800
MalI	GATC	2 cut(s)	597, 879
MbiI	CCGCTC	1 cut(s)	973
MboI	GATC	2 cut(s)	595, 877
MboII	GAAGA	4 cut(s)	53, 90, 557, 1097
MhlI	GDGCHC	1 cut(s)	469
MluCI	AATT	2 cut(s)	502, 919
MlyI	GAGTC	2 cut(s)	90, 169
MmeI	TCCRAC	1 cut(s)	49
MnlI	CCTC	3 cut(s)	116, 189, 1109
Mph1103I	ATGCAT	1 cut(s)	907
MroXI	GAANNNNTTC	1 cut(s)	1107
MseI	TTAA	5 cut(s)	671, 789, 930, 1026, 1208
MspA1I	CMGCKG	1 cut(s)	982
MspCI	CTTAAG	1 cut(s)	1025
MspR9I	CCNGG	1 cut(s)	600
Mva1269I	GAATGC	3 cut(s)	831, 905, 1174
MvaI	CCWGG	1 cut(s)	600
MvnI	CGCG	3 cut(s)	386, 982, 1133
MwoI	GCNNNNNNNGC	4 cut(s)	420, 429, 902, 979
NdeI	CATATG	1 cut(s)	400
NdeII	GATC	2 cut(s)	595, 877
NlaIII	CATG	5 cut(s)	192, 443, 928, 1096, 1177
NlaIV	GGNNCC	2 cut(s)	555, 749
NmuCI	GTSAC	1 cut(s)	725
NotI	GCGGCCGC	1 cut(s)	409
NsiI	ATGCAT	1 cut(s)	907
NspV	TTCGAA	1 cut(s)	144
PagI	TCATGA	1 cut(s)	924
PceI	AGGCCT	2 cut(s)	98, 774
PctI	GAATGC	3 cut(s)	831, 905, 1174
PdmI	GAANNNNTTC	1 cut(s)	1107
PfeI	GAWTC	1 cut(s)	1124
PflMI	CCANNNNNTGG	1 cut(s)	189
PkrI	GCNGC	4 cut(s)	410, 413, 629, 972
PleI	GAGTC	2 cut(s)	89, 169
PmaCI	CACGTG	1 cut(s)	754
PmlI	CACGTG	1 cut(s)	754
PpsI	GAGTC	2 cut(s)	89, 169
Ppu21I	YACGTR	1 cut(s)	754
PshBI	ATTAAT	1 cut(s)	930
Psp6I	CCWGG	1 cut(s)	598
PspCI	CACGTG	1 cut(s)	754
PspFI	CCCAGC	2 cut(s)	117, 182
PspGI	CCWGG	1 cut(s)	598
PspN4I	GGNNCC	2 cut(s)	555, 749
PspPI	GGNCC	3 cut(s)	554, 941, 977
RsaI	GTAC	6 cut(s)	194, 531, 642, 749, 1077, 1136
RsaNI	GTAC	6 cut(s)	193, 530, 641, 748, 1076, 1135
Rsr2I	CGGWCCG	1 cut(s)	977
RsrII	CGGWCCG	1 cut(s)	977
SacII	CCGCGG	1 cut(s)	983
SalI	GTCGAC	1 cut(s)	375
SaqAI	TTAA	5 cut(s)	671, 789, 930, 1026, 1208
SatI	GCNGC	4 cut(s)	409, 412, 628, 971
Sau3AI	GATC	2 cut(s)	595, 877
Sau96I	GGNCC	3 cut(s)	554, 941, 977
ScaI	AGTACT	1 cut(s)	642
SchI	GAGTC	2 cut(s)	90, 169
ScrFI	CCNGG	1 cut(s)	600
SduI	GDGCHC	1 cut(s)	469
SfaNI	GCATC	2 cut(s)	378, 788
SfcI	CTRYAG	1 cut(s)	255
Sfr303I	CCGCGG	1 cut(s)	983
SfuI	TTCGAA	1 cut(s)	144
SgrBI	CCGCGG	1 cut(s)	983
SinI	GGWCC	3 cut(s)	554, 941, 977
SmlI	CTYRAG	2 cut(s)	473, 1025
SmoI	CTYRAG	2 cut(s)	473, 1025
Sse9I	AATT	2 cut(s)	502, 919
SseBI	AGGCCT	2 cut(s)	98, 774
SsiI	CCGC	7 cut(s)	408, 412, 633, 971, 980, 982, 1131
SspMI	CTAG	1 cut(s)	483
StuI	AGGCCT	2 cut(s)	98, 774
StyD4I	CCNGG	1 cut(s)	598
StyI	CCWWGG	1 cut(s)	944
TaaI	ACNGT	6 cut(s)	13, 242, 558, 593, 800, 1162
TaiI	ACGT	4 cut(s)	318, 452, 691, 756
TaqI	TCGA	5 cut(s)	40, 144, 219, 376, 854
TasI	AATT	2 cut(s)	502, 919
TatI	WGTACW	2 cut(s)	640, 1075
TauI	GCSGC	3 cut(s)	411, 414, 973
TfiI	GAWTC	1 cut(s)	1124
Tru1I	TTAA	5 cut(s)	671, 789, 930, 1026, 1208
Tru9I	TTAA	5 cut(s)	671, 789, 930, 1026, 1208
TscAI	CASTG	3 cut(s)	304, 598, 610
TseFI	GTSAC	1 cut(s)	725
TseI	GCWGC	1 cut(s)	627
Tsp45I	GTSAC	1 cut(s)	725
TspDTI	ATGAA	5 cut(s)	525, 630, 657, 834, 913
TspGWI	ACGGA	1 cut(s)	1097
TspRI	CASTG	3 cut(s)	304, 598, 610
Van91I	CCANNNNNTGG	1 cut(s)	189
Vha464I	CTTAAG	1 cut(s)	1025
VneI	GTGCAC	1 cut(s)	465
VpaK11BI	GGWCC	3 cut(s)	554, 941, 977
VspI	ATTAAT	1 cut(s)	930
XapI	RAATTY	2 cut(s)	502, 919
XcmI	CCANNNNNNNNNTGG	1 cut(s)	341
XmiI	GTMKAC	1 cut(s)	376
XmnI	GAANNNNTTC	1 cut(s)	1107
XspI	CTAG	1 cut(s)	483
ZrmI	AGTACT	1 cut(s)	642
Zsp2I	ATGCAT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.