Rorug04G0013000

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005808
Physical Location & Seq
Reverse (-)
2044424 .. 2046766
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0013000.1

Sequence Viewer

Length: 693 bp
ATGGAGAAAACTATACCTTTGGCTATATCTGATTGCTTTCAGCGAGGAGTGGGAGATAAAGCAGTTAATCAATTGGAAAAATCAGTCAACTCAAAACTTGATGCTACTGTATCCAGGCAAATCGAGGCACAGTTTCAAACATCGGGTAAACAAGCTCTTCAGGATGCTCTCAAGTCTAGCATGGAAGCTTTAGTGGTACCTCCCTTTGAGAAGTCATGTAAAGCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAGGGAATGGTTGAACATGCAATTGTGGCTCTGCAGCATTTTGAGTCTGCACGTTCACCATTGGCCCATGCTTTAAGGGAAGCAATTAGCTCCGCATCATCAGTGACTCAAACCCTAAGCGGAGAATTGGATGATGGTCAACGATTGTATTATATAATTGGTATACTTGCAAGCAGTGAAGAATATGCTAAAAGTTCTAGTCTGACTTGGCTAAATTCCGCTTCAAGATTCATGCAAGCAAGTTGTTGCTTGAAATCAACTGAACACTACCTAGATTTCTTCACTGGTAGATTTTACCTATATAAAAGTTGGATGGTTACCAGCAAAAATACACTAATATACCTTGATAGTGGCAAGACTCTTCTGATGGTCAGAGCAAGCAAGGGATGTGATCTAACGGGGTGGCTGATCAAGAGTTTGCGGCAAAGGTTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

230

Amino Acids

25.61

Weight (kDa)

8.53

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 418
AciI CCGC 4 cut(s) 348, 375, 474, 676
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 1 cut(s) 198
AgsI TTSAA 4 cut(s) 137, 269, 480, 508
AjnI CCWGG 1 cut(s) 113
AluBI AGCT 3 cut(s) 155, 188, 345
AluI AGCT 3 cut(s) 155, 188, 345
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 1 cut(s) 469
Asp718I GGTACC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 1 cut(s) 303
BanI GGYRCC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 383, 562, 616
BciT130I CCWGG 1 cut(s) 115
BciVI GTATCC 1 cut(s) 121
BclI TGATCA 1 cut(s) 663
BfaI CTAG 3 cut(s) 177, 453, 527
BfmI CTRYAG 1 cut(s) 287
BfuI GTATCC 1 cut(s) 121
BisI GCNGC 2 cut(s) 290, 677
BlsI GCNGC 2 cut(s) 291, 678
Bme1390I CCNGG 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 115
BmsI GCATC 4 cut(s) 91, 154, 232, 359
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 544
BseBI CCWGG 1 cut(s) 115
BseGI GGATG 4 cut(s) 169, 391, 573, 647
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 301
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 1 cut(s) 320
BshNI GGYRCC 1 cut(s) 196
BsnI GGCC 1 cut(s) 320
Bsp143I GATC 2 cut(s) 646, 663
BspACI CCGC 4 cut(s) 348, 375, 474, 676
BspANI GGCC 1 cut(s) 320
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 544
BssMI GATC 2 cut(s) 646, 663
BssNAI GTATAC 1 cut(s) 419
Bst1107I GTATAC 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 2 cut(s) 109, 132
Bst6I CTCTTC 2 cut(s) 162, 621
BstAPI GCANNNNNTGC 1 cut(s) 685
BstC8I GCNNGC 3 cut(s) 427, 492, 634
BstDEI CTNAG 1 cut(s) 371
BstEII GGTNACC 1 cut(s) 571
BstF5I GGATG 4 cut(s) 169, 391, 573, 647
BstKTI GATC 2 cut(s) 649, 666
BstMBI GATC 2 cut(s) 646, 663
BstMWI GCNNNNNNNGC 2 cut(s) 281, 685
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 113
BstSFI CTRYAG 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 419
BsuI GTATCC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 320
BtsCI GGATG 4 cut(s) 169, 391, 573, 647
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 363, 436, 537
Cac8I GCNNGC 3 cut(s) 427, 492, 634
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 1 cut(s) 197
CviAII CATG 6 cut(s) 181, 216, 226, 272, 323, 487
CviJI RGCY 9 cut(s) 23, 155, 188, 224, 284, 320, 345, 466, 661
CviKI_1 RGCY 9 cut(s) 23, 155, 188, 224, 284, 320, 345, 466, 661
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 1 cut(s) 371
DpnI GATC 2 cut(s) 648, 665
DpnII GATC 2 cut(s) 646, 663
Eam1104I CTCTTC 2 cut(s) 162, 621
EarI CTCTTC 2 cut(s) 162, 621
Eco57I CTGAAG 1 cut(s) 143
Eco91I GGTNACC 1 cut(s) 571
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 113
FaeI CATG 6 cut(s) 184, 219, 229, 275, 326, 490
FatI CATG 6 cut(s) 180, 215, 225, 271, 322, 486
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 290, 677
FokI GGATG 4 cut(s) 176, 398, 580, 654
Fsp4HI GCNGC 2 cut(s) 290, 677
FspBI CTAG 3 cut(s) 177, 453, 527
GluI GCNGC 2 cut(s) 290, 677
HaeIII GGCC 1 cut(s) 320
Hin1II CATG 6 cut(s) 184, 219, 229, 275, 326, 490
HincII GTYRAC 2 cut(s) 88, 395
HindII GTYRAC 2 cut(s) 88, 395
HindIII AAGCTT 1 cut(s) 186
HinfI GANTC 4 cut(s) 299, 361, 483, 613
HphI GGTGA 1 cut(s) 303
Hpy166II GTNNAC 5 cut(s) 88, 149, 311, 395, 419
Hpy188I TCNGA 4 cut(s) 31, 459, 621, 629
Hpy188III TCNNGA 4 cut(s) 161, 253, 480, 667
Hpy8I GTNNAC 5 cut(s) 88, 149, 311, 395, 419
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 109, 132
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 6 cut(s) 275, 289, 305, 425, 490, 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 685
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 6 cut(s) 184, 219, 229, 275, 326, 490
KpnI GGTACC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 2 cut(s) 646, 663
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 1 cut(s) 350
LpnPI CCDG 6 cut(s) 100, 127, 146, 266, 525, 589
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 4 cut(s) 91, 154, 232, 359
MaeI CTAG 3 cut(s) 177, 453, 527
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 2 cut(s) 358, 571
MalI GATC 2 cut(s) 648, 665
MboI GATC 2 cut(s) 646, 663
MboII GAAGA 4 cut(s) 149, 446, 526, 608
MfeI CAATTG 2 cut(s) 71, 276
MluCI AATT 6 cut(s) 71, 276, 339, 380, 411, 469
MlyI GAGTC 3 cut(s) 308, 355, 607
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 3 cut(s) 38, 118, 210
MseI TTAA 2 cut(s) 66, 329
MspR9I CCNGG 1 cut(s) 115
MunI CAATTG 2 cut(s) 71, 276
MvaI CCWGG 1 cut(s) 115
MwoI GCNNNNNNNGC 2 cut(s) 281, 685
NdeII GATC 2 cut(s) 646, 663
NlaIII CATG 6 cut(s) 184, 219, 229, 275, 326, 490
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 483
PkrI GCNGC 2 cut(s) 291, 678
PleI GAGTC 3 cut(s) 307, 355, 607
PpsI GAGTC 3 cut(s) 307, 355, 607
Psp6I CCWGG 1 cut(s) 113
PspEI GGTNACC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 291
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 66, 329
SatI GCNGC 2 cut(s) 290, 677
Sau3AI GATC 2 cut(s) 646, 663
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 3 cut(s) 308, 355, 607
ScrFI CCNGG 1 cut(s) 115
SfaNI GCATC 4 cut(s) 91, 154, 232, 359
SfcI CTRYAG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 6 cut(s) 71, 276, 339, 380, 411, 469
SsiI CCGC 4 cut(s) 348, 375, 474, 676
SspMI CTAG 3 cut(s) 177, 453, 527
StyD4I CCNGG 1 cut(s) 113
TaaI ACNGT 2 cut(s) 109, 132
TaiI ACGT 1 cut(s) 310
TaqI TCGA 1 cut(s) 123
TasI AATT 6 cut(s) 71, 276, 339, 380, 411, 469
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 1 cut(s) 483
Tru1I TTAA 2 cut(s) 66, 329
Tru9I TTAA 2 cut(s) 66, 329
TscAI CASTG 3 cut(s) 363, 436, 544
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 475
TspRI CASTG 3 cut(s) 363, 436, 544
XapI RAATTY 1 cut(s) 469
XceI RCATGY 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 418
XspI CTAG 3 cut(s) 177, 453, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.