Rorug04G0012800

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005808
Physical Location & Seq
Reverse (-)
2027108 .. 2027718
611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0012800.1

Sequence Viewer

Length: 447 bp
ATGGGTTTGGAGGAAGATGTGTTAATTGGGTATTCTTTGATTCATTTATGTGCAGGGTGTGGGGACTTGGATTATGCCCAGAAGGTGTTAGATGAAATGCTTGGGAAAAATACTGTGTCGTGGACTAGTTTGATTTGTGGTTATGGTTGGAGGAATATGCCAAAGGAGGCTGTTTTCTTTGTTTTCGAGAGGGTGGCTGCCGGGATTAAGCCCAATTTGGTGACGATGGTGTGCGTCATTTCTGCTTGCGCAAAGTTGAAGGATGTTGCTTTGAGTGAGAGGAGACTGGCTAGTGAAGCAGTTTCGATCATGGGTGAAATGCTACAACAGGGCCTAAGACCCGACATGGTTACCATGTTATCTGCAATTTCAGTTTGTGCACAACTAATAAGTGATTATCTTTCTGGAAAGTTCTGCCATGGTTATGTTCTAAGGAATGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

148

Amino Acids

16.17

Weight (kDa)

5.64

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 36 - 85 4.5e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 250
AgsI TTSAA 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 277
Alw44I GTGCAC 1 cut(s) 378
AoxI GGCC 1 cut(s) 331
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 1 cut(s) 197
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 331
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 232, 326
BaeGI GKGCMC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 220
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 277
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 2 cut(s) 126, 291
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 202
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 1 cut(s) 306
Bsp19I CCATGG 1 cut(s) 418
BspANI GGCC 1 cut(s) 333
BsrI ACTGG 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 115, 444
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 2 cut(s) 335, 431
BstDSI CCRYGG 1 cut(s) 418
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 247
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 331
CseI GACGC 1 cut(s) 223
CviAII CATG 4 cut(s) 310, 346, 355, 419
CviJI RGCY 5 cut(s) 170, 197, 211, 290, 333
CviKI_1 RGCY 5 cut(s) 170, 197, 211, 290, 333
DdeI CTNAG 2 cut(s) 335, 431
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 418
Eco91I GGTNACC 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 331
EcoO65I GGTNACC 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 4 cut(s) 313, 349, 358, 422
FaiI YATR 9 cut(s) 49, 75, 144, 158, 311, 347, 356, 420, 426
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 309, 345, 354, 418
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 126, 291
FspI TGCGCA 1 cut(s) 250
GlaI GCGC 1 cut(s) 250
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 251
Hin1II CATG 4 cut(s) 313, 349, 358, 422
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 1 cut(s) 40
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 232, 326
Hpy166II GTNNAC 2 cut(s) 123, 380
Hpy188III TCNNGA 2 cut(s) 187, 405
Hpy8I GTNNAC 2 cut(s) 123, 380
HpyAV CCTTC 2 cut(s) 76, 253
HpyCH4III ACNGT 2 cut(s) 115, 444
HpyCH4V TGCA 3 cut(s) 53, 365, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 2 cut(s) 335, 431
Hsp92II CATG 4 cut(s) 313, 349, 358, 422
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 1 cut(s) 306
LpnPI CCDG 6 cut(s) 39, 92, 214, 272, 314, 390
Lsp1109I GCAGC 1 cut(s) 184
MaeI CTAG 2 cut(s) 126, 291
MaeIII GTNAC 2 cut(s) 220, 349
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 1 cut(s) 26
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 3 cut(s) 24, 214, 366
MmeI TCCRAC 1 cut(s) 128
MnlI CCTC 5 cut(s) 4, 144, 160, 183, 273
MseI TTAA 2 cut(s) 23, 207
MslI CAYNNNNRTG 2 cut(s) 48, 423
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
MwoI GCNNNNNNNGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 418
NdeII GATC 1 cut(s) 306
NlaIII CATG 4 cut(s) 313, 349, 358, 422
NmuCI GTSAC 1 cut(s) 220
NsbI TGCGCA 1 cut(s) 250
PfeI GAWTC 1 cut(s) 40
PkrI GCNGC 1 cut(s) 199
PspEI GGTNACC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 331
RseI CAYNNNNRTG 2 cut(s) 48, 423
SaqAI TTAA 2 cut(s) 23, 207
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 382
SetI ASST 1 cut(s) 87
SmiMI CAYNNNNRTG 2 cut(s) 48, 423
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 3 cut(s) 24, 214, 366
SspMI CTAG 2 cut(s) 126, 291
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 2 cut(s) 115, 444
TaqI TCGA 2 cut(s) 186, 305
TasI AATT 3 cut(s) 24, 214, 366
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 23, 207
Tru9I TTAA 2 cut(s) 23, 207
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 32, 108
VneI GTGCAC 1 cut(s) 378
XspI CTAG 2 cut(s) 126, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.