Rorug04G0013100

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005808
Physical Location & Seq
Reverse (-)
2046828 .. 2051263
4436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0013100.1

Sequence Viewer

Length: 3621 bp
ATGGCTTCTCTGACCACTAAAGCAGCAGCTAGTTCTTCATCTTCTTCTCTCCCACTTTCTTATACTGATCACCAAAATCATTACACATACGAGGTCTTCCTGAGTTTTCGAGGCGAGGATACGCGCTTTAATTTTACAGATCATCTGCACAGCCTTTTGTGTGACAGGGGAATTGAGACCTTCATAGATGATGAGCTCAAAAGAGGAGAAGAAATATCATCGGCTCTTGTCAAAGCAATTGAGGAGTCAAGAGTTTCAATCATCGTCTTCTCTCAAAATTATGCTTCGTCAAGGTGGTGCTTAGATGAGCTGGTCAAGATACTTGAATGCAGAAAATCCATAGGACAAGAGGTTAGAGCAGTTTTCTACAAGGTGGAACCCTCAGATGTCCGACACCAAAGAGGTGCCTTTGGTGAGGCATTTGCTACGCTTGATCAATGCAAATACAAGGATAGCATGGACAATTGGAAGGCAGCTCTTAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCGAGTCCGAGGCTAAATTTATCAGGAAGATTGTTGGCGAGTTGTCCGCCCAAGTAGTAAACCCTTCATGTGAGTTGCAAGTAGCTGCACATCCAATTGCATTAGAGTCTTGTAGACAAGATGTCAACAGACTTTTAGGTGCAGAGGAAAATATTGTTCGCATGGTAGGCATATGGGGGCCAGGTGGAATAGGAAAGACCACAATTGCGAAAGATGTGTTTAATTCAATTCGCTATGAGTTTGAAAGTAGTTGTTTCTTGGCAGATGTTAGATCTAATTCAAATGGCCTATCCCAACTGCAAGAGAGACTTTTGTTTGATATTTTAAGGGACCCAAATATGAAGGTACGCAGTGTTGATGAAGGAATCAGTTTTATAAAGACAAAGATGCGAAATACAAAAGTTCTCTTAATCTTTGATGACGTGAGTGATTCTAGCCAATTACAGAACTTAGTTCCATCCCCTCATTGTTTTGGGCCAGGCAGTAGAATTCTCATAACAACTAGAGATAAACGTTGGCTAATTGCTCATGAAGTTGATGAAGTATACGAGGTTAAGTTGTTAGATGATCGTTATGCTTTGGAGTTGTTTAGCTTGCATGCATTCAAAAGAAATGGACCTCCAAGTGATTATCTGGAACTTGCACGCCGTGCAGTACGCTATGCCGGAGGCCTTCCATTAGCTTTGACAGTTTTAGGCTCTCATCTATTTGGTAGAAGCAGAGATGAGTGGGAAGCTACATTAGATAGTTGTAAAGGAGAAGATCCCCACATAAAGATAAGAAATGTTCTCAAAATAAGTTATGATGCTCTGGGTTTAGATTTAAAAGGATATTTTCTTGATATCGCTTGTTTCTTTAAAGGTAAGGATGTAGACTGTGTGAAACCAATACTAGAAGCTTGCTACGACCTCAAATCAGTGAGTGGTATTGCACAACTCCAACAAAAGGCCTTAATAAGGATTGACGAGACTTTGATGGGTGGTAGAATATGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTATCAAGAGTCACCTGGTGAGCCCGGGAAACGCAGCAGAGTGTGGAGTGAAGAAGATATCAACCACATTCTAACAAATAATACAGGAACAAATAAAATTATAGGCATTCAGGTCCCGTGGCGGTCATCTACGATATTTTTGAATGCTAAAACCTTTTCAAAGATGAAGAATCTTAGATATATTTCTATGGGCACGTACCTGAAATTTGAATCTTTTTATGGAGACATTGGTTATCTCTCAAACCAGTTGAGGTGGCTTGATTGGCCTGATAGTCCGTTGCAATCTTTTCCATCCGATTTTCATGCAAACAAACTTGTAAAGCTCAATATTCCTGACAGCTGCGGAATCACACGACTATGGGAGGGACGTAAGAATTTTCCAATTTTAACATCTATGGATTTAAGAGGTTGTAAATCCCTCATAGACCTCCCGAACTTCACTGGAATGCCCAACTTAATAGAGTTGAAGTTAGATGGTTGTGAAAGTTTAGTTGAGGTTCATCATTCTGTTGGATTCCTAAATAAGCTTGTGACCTTAAGTCTTAAAGGCTGCTGTAACCTAGTTAAGTTGCCAACCGAATTTAGCTTGAAATCTCTCCTTGCTATGGATCTTAGTGATTGCACTAGGCTAGAGGAATTTCCAAAAATTATTGGAAAGATGGATTCTTTCGGAGTGTTGATCCTATCATTCACTCGTGTTAAAGAATTGCATCCGTCAATTGGAAATCTCATTGGGCTTAATCAGTTATGTCTAGTAGATTGTAAAAATCTTACAACTCTACCATGCAGCATATATGAGTTACAAAATCTAGAGATCTTAGATGTGAGTGGATGCTCAAAATTTGATACATTTCCAGAAATCAAGAGAAAGATGAATTCCTTAAAAAAGTTATATCTAAGAGGCAGTGGCATTACAGAATTGCATCCCTCAATTGGAAATCTCATTCGGCTTAATGAGTTTGATCTAGCAGATAGTGAAAATCTTACAACAATACCATGCAGCATATATGAGTTACAAAATCTAGAGATCCTAAATGTGAGTAGATGCTCAAATCTTGTTACATTTCCAACAAAAGCAAGTATTTCACATGATCATGACAGTGGCTCGCTAGCGCTTCCCCAGCTACGTGTACTCAAAATCAAAGGATGTAATTTATCAACTTTTGATTTCATTGGGAGTCTTGACTGCTTGAAAACATTAACCGAACTTGATCTCTCAAGTAACAATTTTGTTAGTGTTCCTGCACTTAGCAAATTTGTCAACTTGCCAAGGATTGACTTGTATTGTTGCAAGAGACTTCGAGAGATTCCAGAGCTTTCAGCAAATATACTTGAGGTAAATGCATGGGATTGCGAATCGTTGGAGAGATTTTCGATATTGCCCAAGTCTTTGAATATGCTAGAGATGAACTTGTGGAATTGCCATAGATTTAGTTACAGTCTGGGCTATGACATGGAGAAGATGAAAAATATTTTGCTGAATAATCCGAACAACTCCCCGTTTACGCTTGTACTACCTGGTAGTGAGGTTCCGAAGTGGTTCCATATTTCAAAGGAGGTTGCTGCTTATGAAAAAGGCCTGGCATTGACATGTGCAGTTTCTTTTGAAATACCAAGCAAATTGAATTGGGAGACCATAGGACTGGCTCTTTGTTCTGTATTAGTTGAGGAAACTTTTAGTATTTTTATTGACGATCGAGTTGTTATTTTCATCAATGAGGAACTCATAGATCAGAGATCCAATCGGCACAGTCCAACGCCAATTGGTCATATGCGTCTGAATTATATTCCATTATGTGATGAAATAAAGGGAAAGGTGGATCAAAACGGTTGGTTGCGTTATCACTGTCGAGTTCAATTTTACCCTGTTGATCGAATGAAAAGCTGTGGAGTTCACCTCGTATGCCAACCACCGAATGAATATTCAAATAAGATTGTAATGGCGGCTCCACCTGCCGATGAGAGTGGTATGCTAGACTGCTTTTCGTGGCTTTCGTCGCCGTCAATTGAAGATGATTGTGAATCCTTGGACACAGACGTTGGCCTAGGCATAAGACGTTGGCTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

1206

Amino Acids

136.19

Weight (kDa)

5.77

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 30 - 191 1e-51 TIR domain
TIR_2 PF13676 32 - 124 1.2e-13 TIR domain
NB-ARC PF00931 217 - 377 4.2e-18 NB-ARC domain
WHD_ROQ1 PF23282 450 - 521 5.3e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 713 - 797 1.3e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 784 - 983 6.1e-23 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 807 - 980 1.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AarI CACCTGC 1 cut(s) 3511
Acc36I ACCTGC 1 cut(s) 3511
AccB1I GGYRCC 1 cut(s) 404
AccI GTMKAC 3 cut(s) 634, 1065, 1392
AccII CGCG 1 cut(s) 124
AciI CCGC 4 cut(s) 567, 1672, 1893, 3494
AclI AACGTT 1 cut(s) 1033
AclWI GGATC 6 cut(s) 1277, 2166, 2224, 2572, 3282, 3378
AcsI RAATTY 9 cut(s) 536, 1008, 1754, 1924, 2129, 2186, 2390, 2425, 2804
AdeI CACNNNGTG 2 cut(s) 1169, 1568
AfaI GTAC 5 cut(s) 867, 1176, 1748, 2682, 3063
AfeI AGCGCT 1 cut(s) 2664
AfiI CCNNNNNNNGG 5 cut(s) 1465, 1476, 2155, 2270, 2483
AflII CTTAAG 2 cut(s) 479, 2086
AflIII ACRYGT 2 cut(s) 2677, 3140
AhdI GACNNNNNGTC 2 cut(s) 641, 2088
AjiI CACGTC 1 cut(s) 943
AjnI CCWGG 5 cut(s) 700, 997, 1564, 3067, 3129
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 6 cut(s) 170, 820, 1481, 1767, 2839, 3176
AlwI GGATC 6 cut(s) 1277, 2166, 2224, 2572, 3282, 3378
Ama87I CYCGRG 1 cut(s) 1574
Aor51HI AGCGCT 1 cut(s) 2664
AoxI GGCC 9 cut(s) 506, 698, 805, 995, 1189, 1467, 1814, 3127, 3592
ApoI RAATTY 9 cut(s) 536, 1008, 1754, 1924, 2129, 2186, 2390, 2425, 2804
ArsI GACNNNNNNTTYG 2 cut(s) 3183, 3215
Asp700I GAANNNNTTC 1 cut(s) 3089
AspA2I CCTAGG 1 cut(s) 3595
AspLEI GCGC 2 cut(s) 126, 2665
AspS9I GGNCC 5 cut(s) 698, 850, 995, 1136, 1663
AsuC2I CCSGG 2 cut(s) 1575, 1576
AsuHPI GGTGA 5 cut(s) 62, 425, 1554, 1580, 3437
AsuNHI GCTAGC 1 cut(s) 2659
AvaI CYCGRG 1 cut(s) 1574
AvaII GGWCC 3 cut(s) 850, 1136, 1663
AvrII CCTAGG 1 cut(s) 3595
BaeGI GKGCMC 1 cut(s) 1745
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 2 cut(s) 198, 1575
BauI CACGAG 1 cut(s) 2244
BbsI GAAGAC 2 cut(s) 88, 259
Bbv12I GWGCWC 1 cut(s) 198
BccI CCATC 7 cut(s) 498, 512, 985, 1489, 1849, 2018, 2203
BceAI ACGGC 2 cut(s) 1152, 3535
BcgI CGANNNNNNTGC 2 cut(s) 1835, 1869
BciT130I CCWGG 5 cut(s) 702, 999, 1566, 3069, 3131
BciVI GTATCC 1 cut(s) 112
BclI TGATCA 3 cut(s) 67, 433, 2641
BcnI CCSGG 2 cut(s) 1575, 1576
BcoDI GTCTC 6 cut(s) 170, 820, 1481, 1767, 2839, 3176
BfoI RGCGCY 1 cut(s) 2666
BfrI CTTAAG 2 cut(s) 479, 2086
BfuAI ACCTGC 1 cut(s) 3511
BfuI GTATCC 1 cut(s) 112
BglII AGATCT 2 cut(s) 791, 2364
BlnI CCTAGG 1 cut(s) 3595
Bme1390I CCNGG 7 cut(s) 702, 999, 1566, 1575, 1576, 3069, 3131
Bme18I GGWCC 3 cut(s) 850, 1136, 1663
BmeRI GACNNNNNGTC 2 cut(s) 641, 2088
BmeT110I CYCGRG 1 cut(s) 1574
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 5 cut(s) 698, 850, 995, 1136, 1663
BmiI GGNNCC 9 cut(s) 378, 406, 699, 851, 852, 1665, 3081, 3092, 3498
BmrFI CCNGG 7 cut(s) 702, 999, 1566, 1575, 1576, 3069, 3131
BmsI GCATC 7 cut(s) 897, 1315, 1503, 2269, 2372, 2482, 2585
BmtI GCTAGC 1 cut(s) 2663
BpiI GAAGAC 2 cut(s) 88, 259
BplI GAGNNNNNCTC 2 cut(s) 3432, 3464
BpuEI CTTGAG 2 cut(s) 2752, 2903
BpuMI CCSGG 2 cut(s) 1575, 1576
BsaAI YACGTR 2 cut(s) 1746, 2678
BsaI GGTCTC 2 cut(s) 170, 3176
BsaJI CCNNGG 6 cut(s) 528, 1574, 1667, 2819, 3576, 3595
Bsc4I CCNNNNNNNGG 5 cut(s) 1465, 1476, 2155, 2270, 2483
Bse1I ACTGG 3 cut(s) 1795, 1996, 3198
BseBI CCWGG 5 cut(s) 702, 999, 1566, 3069, 3131
BseDI CCNNGG 6 cut(s) 528, 1574, 1667, 2819, 3576, 3595
BseLI CCNNNNNNNGG 5 cut(s) 1465, 1476, 2155, 2270, 2483
BseMII CTCAG 2 cut(s) 92, 396
BseNI ACTGG 3 cut(s) 1795, 1996, 3198
BseRI GAGGAG 2 cut(s) 219, 257
BseSI GKGCMC 1 cut(s) 1745
BseYI CCCAGC 1 cut(s) 2670
BsgI GTGCAG 6 cut(s) 131, 591, 681, 1191, 2778, 3165
Bsh1236I CGCG 1 cut(s) 124
Bsh1285I CGRYCG 1 cut(s) 3247
BshFI GGCC 9 cut(s) 508, 700, 807, 997, 1191, 1469, 1816, 3129, 3594
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 3247
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 1574
BsiSI CCGG 2 cut(s) 1185, 1575
BslFI GGGAC 3 cut(s) 863, 1649, 1929
BslI CCNNNNNNNGG 5 cut(s) 1465, 1476, 2155, 2270, 2483
BsmAI GTCTC 6 cut(s) 170, 820, 1481, 1767, 2839, 3176
BsmFI GGGAC 3 cut(s) 863, 1649, 1929
BsmI GAATGC 5 cut(s) 332, 1121, 1656, 1699, 2001
BsnI GGCC 9 cut(s) 508, 700, 807, 997, 1191, 1469, 1816, 3129, 3594
Bso31I GGTCTC 2 cut(s) 170, 3176
BsoBI CYCGRG 1 cut(s) 1574
Bsp1286I GDGCHC 3 cut(s) 198, 1575, 1745
BspACI CCGC 4 cut(s) 567, 1672, 1893, 3494
BspANI GGCC 9 cut(s) 508, 700, 807, 997, 1191, 1469, 1816, 3129, 3594
BspCNI CTCAG 2 cut(s) 93, 395
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 2 cut(s) 1048, 2644
BspLI GGNNCC 9 cut(s) 378, 406, 699, 851, 852, 1665, 3081, 3092, 3498
BspMI ACCTGC 1 cut(s) 3511
BspOI GCTAGC 1 cut(s) 2663
BspPI GGATC 6 cut(s) 1277, 2166, 2224, 2572, 3282, 3378
BspT107I GGYRCC 1 cut(s) 404
BspTI CTTAAG 2 cut(s) 479, 2086
BspTNI GGTCTC 2 cut(s) 170, 3176
BsrI ACTGG 3 cut(s) 1795, 1996, 3198
BssECI CCNNGG 6 cut(s) 528, 1574, 1667, 2819, 3576, 3595
BssNAI GTATAC 1 cut(s) 1066
BssSI CACGAG 1 cut(s) 2244
BssT1I CCWWGG 3 cut(s) 2819, 3576, 3595
Bst1107I GTATAC 1 cut(s) 1066
Bst2BI CACGAG 1 cut(s) 2244
Bst2UI CCWGG 5 cut(s) 702, 999, 1566, 3069, 3131
Bst4CI ACNGT 7 cut(s) 1210, 1397, 2651, 2990, 3302, 3380, 3398
BstAFI CTTAAG 2 cut(s) 479, 2086
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstBAI YACGTR 2 cut(s) 1746, 2678
BstC8I GCNNGC 6 cut(s) 1115, 1119, 1165, 1420, 2657, 2661
BstDEI CTNAG 9 cut(s) 101, 301, 382, 970, 1724, 2162, 2368, 2447, 2798
BstDSI CCRYGG 1 cut(s) 1667
BstENI CCTNNNNNAGG 1 cut(s) 1474
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 1 cut(s) 2666
BstHHI GCGC 2 cut(s) 126, 2665
BstMAI GTCTC 6 cut(s) 170, 820, 1481, 1767, 2839, 3176
BstMCI CGRYCG 1 cut(s) 3247
BstMWI GCNNNNNNNGC 6 cut(s) 687, 1169, 1813, 2671, 3503, 3547
BstNI CCWGG 5 cut(s) 702, 999, 1566, 3069, 3131
BstNSI RCATGY 2 cut(s) 1121, 3144
BstSCI CCNGG 7 cut(s) 700, 997, 1564, 1573, 1574, 3067, 3129
BstSLI GKGCMC 1 cut(s) 1745
BstUI CGCG 1 cut(s) 124
BstV2I GAAGAC 2 cut(s) 88, 259
BstX2I RGATCY 6 cut(s) 791, 1282, 2158, 2364, 2577, 3287
BstXI CCANNNNNNTGG 1 cut(s) 3193
BstYI RGATCY 6 cut(s) 791, 1282, 2158, 2364, 2577, 3287
BstZ17I GTATAC 1 cut(s) 1066
BsuI GTATCC 1 cut(s) 112
BsuRI GGCC 9 cut(s) 508, 700, 807, 997, 1191, 1469, 1816, 3129, 3594
BtgI CCRYGG 1 cut(s) 1667
BtrI CACGTC 1 cut(s) 943
BtsI GCAGTG 2 cut(s) 877, 2461
BtsIMutI CAGTG 6 cut(s) 877, 1443, 1989, 2461, 2656, 3394
BveI ACCTGC 1 cut(s) 3511
Cac8I GCNNGC 6 cut(s) 1115, 1119, 1165, 1420, 2657, 2661
CciI TCATGA 2 cut(s) 1048, 2644
CfoI GCGC 2 cut(s) 126, 2665
Cfr13I GGNCC 5 cut(s) 698, 850, 995, 1136, 1663
Cfr9I CCCGGG 1 cut(s) 1574
CseI GACGC 1 cut(s) 3314
CsiI ACCWGGT 2 cut(s) 1564, 3067
Csp6I GTAC 5 cut(s) 866, 1175, 1747, 2681, 3062
CviQI GTAC 5 cut(s) 866, 1175, 1747, 2681, 3062
DdeI CTNAG 9 cut(s) 101, 301, 382, 970, 1724, 2162, 2368, 2447, 2798
DraI TTTAAA 2 cut(s) 1344, 1378
DraIII CACNNNGTG 2 cut(s) 1169, 1568
DriI GACNNNNNGTC 2 cut(s) 641, 2088
Eam1105I GACNNNNNGTC 2 cut(s) 641, 2088
EciI GGCGGA 1 cut(s) 556
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 3 cut(s) 2819, 3576, 3595
Eco147I AGGCCT 3 cut(s) 1191, 1469, 3129
Eco24I GRGCYC 2 cut(s) 198, 1575
Eco31I GGTCTC 2 cut(s) 170, 3176
Eco32I GATATC 2 cut(s) 1363, 1609
Eco47I GGWCC 3 cut(s) 850, 1136, 1663
Eco47III AGCGCT 1 cut(s) 2664
Eco53kI GAGCTC 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 1574
EcoICRI GAGCTC 1 cut(s) 196
EcoNI CCTNNNNNAGG 1 cut(s) 1474
EcoO109I RGGNCCY 2 cut(s) 850, 1663
EcoRI GAATTC 2 cut(s) 1008, 2425
EcoRII CCWGG 5 cut(s) 700, 997, 1564, 3067, 3129
EcoRV GATATC 2 cut(s) 1363, 1609
EcoT14I CCWWGG 3 cut(s) 2819, 3576, 3595
EcoT22I ATGCAT 3 cut(s) 1123, 1518, 2896
EcoT38I GRGCYC 2 cut(s) 198, 1575
ErhI CCWWGG 3 cut(s) 2819, 3576, 3595
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 3 cut(s) 863, 1649, 1929
FauNDI CATATG 2 cut(s) 692, 3321
FbaI TGATCA 3 cut(s) 67, 433, 2641
FblI GTMKAC 3 cut(s) 634, 1065, 1392
FriOI GRGCYC 2 cut(s) 198, 1575
GlaI GCGC 2 cut(s) 125, 2664
GsaI CCCAGC 1 cut(s) 2674
HaeII RGCGCY 1 cut(s) 2666
HaeIII GGCC 9 cut(s) 508, 700, 807, 997, 1191, 1469, 1816, 3129, 3594
HapII CCGG 2 cut(s) 1185, 1575
HgaI GACGC 1 cut(s) 3314
HhaI GCGC 2 cut(s) 126, 2665
Hin6I GCGC 2 cut(s) 124, 2663
HinP1I GCGC 2 cut(s) 124, 2663
HincII GTYRAC 2 cut(s) 646, 2812
HindII GTYRAC 2 cut(s) 646, 2812
HindIII AAGCTT 2 cut(s) 1416, 2075
HpaII CCGG 2 cut(s) 1185, 1575
HphI GGTGA 5 cut(s) 62, 425, 1554, 1580, 3437
Hpy166II GTNNAC 9 cut(s) 580, 635, 646, 1066, 1393, 2681, 2812, 3054, 3445
Hpy8I GTNNAC 9 cut(s) 580, 635, 646, 1066, 1393, 2681, 2812, 3054, 3445
Hpy99I CGWCG 1 cut(s) 3550
HpyAV CCTTC 6 cut(s) 190, 463, 594, 856, 875, 1202
HpyCH4III ACNGT 7 cut(s) 1210, 1397, 2651, 2990, 3302, 3380, 3398
HpyCH4IV ACGT 7 cut(s) 942, 1033, 1745, 1918, 2677, 3588, 3607
HpyF10VI GCNNNNNNNGC 6 cut(s) 687, 1169, 1813, 2671, 3503, 3547
HpyF3I CTNAG 9 cut(s) 101, 301, 382, 970, 1724, 2162, 2368, 2447, 2798
HpySE526I ACGT 7 cut(s) 942, 1033, 1745, 1918, 2677, 3588, 3607
HspAI GCGC 2 cut(s) 124, 2663
KflI GGGWCCC 1 cut(s) 850
Ksp22I TGATCA 3 cut(s) 67, 433, 2641
LmnI GCTCC 1 cut(s) 3502
LweI GCATC 7 cut(s) 897, 1315, 1503, 2269, 2372, 2482, 2585
MabI ACCWGGT 2 cut(s) 1564, 3067
MaeII ACGT 7 cut(s) 942, 1033, 1745, 1918, 2677, 3588, 3607
MaeIII GTNAC 9 cut(s) 161, 1560, 2080, 2105, 2349, 2562, 2608, 2771, 2984
MfeI CAATTG 8 cut(s) 237, 463, 615, 723, 2268, 2481, 3312, 3555
MflI RGATCY 6 cut(s) 791, 1282, 2158, 2364, 2577, 3287
MhlI GDGCHC 3 cut(s) 198, 1575, 1745
MlyI GAGTC 5 cut(s) 254, 533, 635, 1568, 2737
MmeI TCCRAC 6 cut(s) 415, 1483, 2041, 2642, 2892, 3329
Mph1103I ATGCAT 3 cut(s) 1123, 1518, 2896
MroXI GAANNNNTTC 1 cut(s) 3089
MslI CAYNNNNRTG 5 cut(s) 1906, 1994, 2643, 2649, 3139
MspA1I CMGCKG 1 cut(s) 1890
MspCI CTTAAG 2 cut(s) 479, 2086
MspI CCGG 2 cut(s) 1185, 1575
MspR9I CCNGG 7 cut(s) 702, 999, 1566, 1575, 1576, 3069, 3131
MunI CAATTG 8 cut(s) 237, 463, 615, 723, 2268, 2481, 3312, 3555
Mva1269I GAATGC 5 cut(s) 332, 1121, 1656, 1699, 2001
MvaI CCWGG 5 cut(s) 702, 999, 1566, 3069, 3131
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 6 cut(s) 687, 1169, 1813, 2671, 3503, 3547
NciI CCSGG 2 cut(s) 1575, 1576
NdeI CATATG 2 cut(s) 692, 3321
NheI GCTAGC 1 cut(s) 2659
NlaIV GGNNCC 9 cut(s) 378, 406, 699, 851, 852, 1665, 3081, 3092, 3498
NmuCI GTSAC 3 cut(s) 161, 1560, 2080
NsiI ATGCAT 3 cut(s) 1123, 1518, 2896
NspI RCATGY 2 cut(s) 1121, 3144
PaeI GCATGC 1 cut(s) 1121
PagI TCATGA 2 cut(s) 1048, 2644
PaqCI CACCTGC 1 cut(s) 3511
PceI AGGCCT 3 cut(s) 1191, 1469, 3129
PciI ACATGT 1 cut(s) 3140
PcsI WCGNNNNNNNCGW 1 cut(s) 3542
PctI GAATGC 5 cut(s) 332, 1121, 1656, 1699, 2001
PdmI GAANNNNTTC 1 cut(s) 3089
Ple19I CGATCG 1 cut(s) 3247
PleI GAGTC 5 cut(s) 253, 532, 634, 1567, 2736
PpsI GAGTC 5 cut(s) 253, 532, 634, 1567, 2736
Ppu21I YACGTR 2 cut(s) 1746, 2678
PpuMI RGGWCCY 2 cut(s) 850, 1663
PscI ACATGT 1 cut(s) 3140
PsiI TTATAA 1 cut(s) 896
Psp124BI GAGCTC 1 cut(s) 198
Psp1406I AACGTT 1 cut(s) 1033
Psp5II RGGWCCY 2 cut(s) 850, 1663
Psp6I CCWGG 5 cut(s) 700, 997, 1564, 3067, 3129
PspFI CCCAGC 1 cut(s) 2670
PspGI CCWGG 5 cut(s) 700, 997, 1564, 3067, 3129
PspN4I GGNNCC 9 cut(s) 378, 406, 699, 851, 852, 1665, 3081, 3092, 3498
PspPI GGNCC 5 cut(s) 698, 850, 995, 1136, 1663
PspPPI RGGWCCY 2 cut(s) 850, 1663
PsuI RGATCY 6 cut(s) 791, 1282, 2158, 2364, 2577, 3287
PvuI CGATCG 1 cut(s) 3247
PvuII CAGCTG 1 cut(s) 1890
RsaI GTAC 5 cut(s) 867, 1176, 1748, 2682, 3063
RsaNI GTAC 5 cut(s) 866, 1175, 1747, 2681, 3062
RseI CAYNNNNRTG 5 cut(s) 1906, 1994, 2643, 2649, 3139
SacI GAGCTC 1 cut(s) 198
Sau96I GGNCC 5 cut(s) 698, 850, 995, 1136, 1663
SchI GAGTC 5 cut(s) 254, 533, 635, 1568, 2737
ScrFI CCNGG 7 cut(s) 702, 999, 1566, 1575, 1576, 3069, 3131
SduI GDGCHC 3 cut(s) 198, 1575, 1745
SexAI ACCWGGT 2 cut(s) 1564, 3067
SfaNI GCATC 7 cut(s) 897, 1315, 1503, 2269, 2372, 2482, 2585
SinI GGWCC 3 cut(s) 850, 1136, 1663
SmaI CCCGGG 1 cut(s) 1576
SmiMI CAYNNNNRTG 5 cut(s) 1906, 1994, 2643, 2649, 3139
SmlI CTYRAG 4 cut(s) 479, 2086, 2767, 2882
SmoI CTYRAG 4 cut(s) 479, 2086, 2767, 2882
SphI GCATGC 1 cut(s) 1121
SseBI AGGCCT 3 cut(s) 1191, 1469, 3129
SsiI CCGC 4 cut(s) 567, 1672, 1893, 3494
SspI AATATT 4 cut(s) 673, 1879, 3022, 3473
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 3 cut(s) 1191, 1469, 3129
StyD4I CCNGG 7 cut(s) 700, 997, 1564, 1573, 1574, 3067, 3129
StyI CCWWGG 3 cut(s) 2819, 3576, 3595
TaaI ACNGT 7 cut(s) 1210, 1397, 2651, 2990, 3302, 3380, 3398
TaiI ACGT 7 cut(s) 945, 1036, 1748, 1921, 2680, 3591, 3610
TaqI TCGA 6 cut(s) 109, 2851, 2924, 3247, 3400, 3424
TatI WGTACW 2 cut(s) 2680, 3061
TauI GCSGC 1 cut(s) 3497
TscAI CASTG 6 cut(s) 877, 1443, 1996, 2461, 2656, 3401
TseFI GTSAC 3 cut(s) 161, 1560, 2080
Tsp45I GTSAC 3 cut(s) 161, 1560, 2080
TspGWI ACGGA 2 cut(s) 1815, 2253
TspMI CCCGGG 1 cut(s) 1574
TspRI CASTG 6 cut(s) 877, 1443, 1996, 2461, 2656, 3401
Vha464I CTTAAG 2 cut(s) 479, 2086
VpaK11BI GGWCC 3 cut(s) 850, 1136, 1663
XagI CCTNNNNNAGG 1 cut(s) 1474
XapI RAATTY 9 cut(s) 536, 1008, 1754, 1924, 2129, 2186, 2390, 2425, 2804
XbaI TCTAGA 2 cut(s) 2359, 2572
XceI RCATGY 2 cut(s) 1121, 3144
XmaI CCCGGG 1 cut(s) 1574
XmaJI CCTAGG 1 cut(s) 3595
XmiI GTMKAC 3 cut(s) 634, 1065, 1392
XmnI GAANNNNTTC 1 cut(s) 3089
Zsp2I ATGCAT 3 cut(s) 1123, 1518, 2896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.