AT4G31310

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010238
Physical Location & Seq
Forward (+)
15191220 .. 15192596
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31310.2

Sequence Viewer

Length: 519 bp
ATGTTGTCAGGGAAAGCTGCTTACGATCTCTTTGTCTATGGCAGTCTCCAGGAACCCGAAGTCGTATACGTGCTTCTTAATCGCGTCCCTGACCATGTCTCCGCCGTACTCTCCGGCTTTCACAGGTTTAGACTCAAAGGCCGTGTCTATCCAACAATTCTACCGGACGGTACCGGAAAAGTCAACGGAAAGGTGCTAAAGGGCATAACAGATGATGAACTGAAGATGTTAGATGAGTTTGAAGATGTTGAATATGACAGAAAGACTGTTGAAGTAATGCTGACGGATACTTCAGAGAAGCTGCAAGTCGAAACATATGTATGGAAGAACAAAGATGATCCGGATCTTTATGGAGAATGGGATTTCGAGGAATGGAGACAACATGACAAGGAAGATTTCGTAACAGCGACCAAGAAGTTTCTGGAAAATAGGAGATTACCAGAAGCAAAGACGAGGATGGACACATTCAAAACATTCTTTAAGCAGGATCTTGAGAATGGGAAACCTCTGGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

172

Amino Acids

20.12

Weight (kDa)

5.01

Isoelectric Point (pI)

22.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 12 - 123 4.6e-24 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 170
AccB1I GGYRCC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 1 cut(s) 102
AclWI GGATC 3 cut(s) 332, 351, 495
AcuI CTGAAG 2 cut(s) 242, 276
AfaI GTAC 2 cut(s) 108, 172
AgsI TTSAA 4 cut(s) 242, 251, 272, 469
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 2 cut(s) 17, 301
AluI AGCT 2 cut(s) 17, 301
Alw26I GTCTC 3 cut(s) 50, 103, 370
AlwI GGATC 3 cut(s) 332, 351, 495
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 17, 301
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
Asp718I GGTACC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 170
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BbvI GCAGC 2 cut(s) 4, 288
BccI CCATC 1 cut(s) 451
BceAI ACGGC 2 cut(s) 89, 126
BciT130I CCWGG 1 cut(s) 50
BciVI GTATCC 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 50, 103, 370
BfuI GTATCC 1 cut(s) 280
BisI GCNGC 2 cut(s) 18, 302
BlsI GCNGC 2 cut(s) 19, 303
Bme1390I CCNGG 1 cut(s) 50
BmiI GGNNCC 2 cut(s) 54, 172
BmrFI CCNGG 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 342
BsaWI WCCGGW 3 cut(s) 163, 173, 340
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 1 cut(s) 50
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 1 cut(s) 342
BseXI GCAGC 2 cut(s) 4, 288
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 141
BshNI GGYRCC 1 cut(s) 170
BsiSI CCGG 4 cut(s) 114, 164, 174, 341
BslFI GGGAC 1 cut(s) 71
BsmAI GTCTC 3 cut(s) 50, 103, 370
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 4 cut(s) 25, 337, 343, 487
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 141
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 54, 172
BspPI GGATC 3 cut(s) 332, 351, 495
BspT107I GGYRCC 1 cut(s) 170
BssMI GATC 4 cut(s) 25, 337, 343, 487
BssNAI GTATAC 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 170, 268
BstBAI YACGTR 1 cut(s) 70
BstF5I GGATG 1 cut(s) 462
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 4 cut(s) 28, 340, 346, 490
BstMAI GTCTC 3 cut(s) 50, 103, 370
BstMBI GATC 4 cut(s) 25, 337, 343, 487
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 4, 288
BstX2I RGATCY 2 cut(s) 343, 487
BstYI RGATCY 2 cut(s) 343, 487
BstZ17I GTATAC 1 cut(s) 67
BsuI GTATCC 1 cut(s) 280
BsuRI GGCC 1 cut(s) 141
BtsCI GGATG 1 cut(s) 462
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 107, 171
CviAII CATG 2 cut(s) 95, 383
CviJI RGCY 4 cut(s) 17, 117, 141, 301
CviKI_1 RGCY 4 cut(s) 17, 117, 141, 301
CviQI GTAC 2 cut(s) 107, 171
DpnI GATC 4 cut(s) 27, 339, 345, 489
DpnII GATC 4 cut(s) 25, 337, 343, 487
EciI GGCGGA 1 cut(s) 91
Eco57I CTGAAG 2 cut(s) 242, 276
EcoRII CCWGG 1 cut(s) 48
FaeI CATG 2 cut(s) 98, 386
FaqI GGGAC 1 cut(s) 71
FatI CATG 2 cut(s) 94, 382
FauNDI CATATG 1 cut(s) 316
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 18, 302
FokI GGATG 1 cut(s) 469
Fsp4HI GCNGC 2 cut(s) 18, 302
GluI GCNGC 2 cut(s) 18, 302
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 1 cut(s) 141
HapII CCGG 4 cut(s) 114, 164, 174, 341
HgaI GACGC 1 cut(s) 73
Hin1II CATG 2 cut(s) 98, 386
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 2 cut(s) 132, 512
HpaII CCGG 4 cut(s) 114, 164, 174, 341
Hpy166II GTNNAC 2 cut(s) 67, 184
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 5 cut(s) 341, 422, 491, 509, 516
Hpy8I GTNNAC 2 cut(s) 67, 184
HpyCH4III ACNGT 2 cut(s) 170, 268
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 304
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 98, 386
Kpn2I TCCGGA 1 cut(s) 340
KpnI GGTACC 1 cut(s) 174
Kzo9I GATC 4 cut(s) 25, 337, 343, 487
Lsp1109I GCAGC 2 cut(s) 4, 288
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 400
MalI GATC 4 cut(s) 27, 339, 345, 489
MboI GATC 4 cut(s) 25, 337, 343, 487
MboII GAAGA 4 cut(s) 235, 254, 337, 404
MflI RGATCY 2 cut(s) 343, 487
MluCI AATT 1 cut(s) 156
MlyI GAGTC 2 cut(s) 126, 506
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 3 cut(s) 361, 447, 516
MroI TCCGGA 1 cut(s) 340
MseI TTAA 2 cut(s) 78, 480
MslI CAYNNNNRTG 1 cut(s) 319
MspI CCGG 4 cut(s) 114, 164, 174, 341
MspR9I CCNGG 1 cut(s) 50
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 84
NdeI CATATG 1 cut(s) 316
NdeII GATC 4 cut(s) 25, 337, 343, 487
NlaIII CATG 2 cut(s) 98, 386
NlaIV GGNNCC 2 cut(s) 54, 172
PflFI GACNNNGTC 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 2 cut(s) 19, 303
PleI GAGTC 2 cut(s) 126, 506
PpsI GAGTC 2 cut(s) 126, 506
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 54, 172
PsuI RGATCY 2 cut(s) 343, 487
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 2 cut(s) 108, 172
RsaNI GTAC 2 cut(s) 107, 171
RseI CAYNNNNRTG 1 cut(s) 319
SaqAI TTAA 2 cut(s) 78, 480
SatI GCNGC 2 cut(s) 18, 302
Sau3AI GATC 4 cut(s) 25, 337, 343, 487
SchI GAGTC 2 cut(s) 126, 506
ScrFI CCNGG 1 cut(s) 50
SetI ASST 6 cut(s) 19, 72, 128, 195, 303, 508
SmiMI CAYNNNNRTG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 1 cut(s) 156
SsiI CCGC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 170, 268
TaiI ACGT 1 cut(s) 72
TaqI TCGA 2 cut(s) 309, 366
TasI AATT 1 cut(s) 156
Tru1I TTAA 2 cut(s) 78, 480
Tru9I TTAA 2 cut(s) 78, 480
TseI GCWGC 2 cut(s) 17, 301
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 2 cut(s) 201, 299
Tth111I GACNNNGTC 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.