FvH4_2g36470

AIG2-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010238
Physical Location & Seq
Reverse (-)
26698457 .. 26700184
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36470.t1

Sequence Viewer

Length: 513 bp
ATGAGTTCAGTGGCGATGGCGAACTCTCCGAGCGTCGGCCACAACGTGTTCGTCTACGGCAGCCTGATGGCGGAGGACGTCTGTCGAGTCCTCTTGAATCGAGTCCCTCAATCCGCTACTGCTACTCTCAATGGCTATCATAGGTACAGCATCAAAGGACGTGTTTATCCGGCGATTCTACCGGTTGAGAATAAGAAGGTTACCGGCAAGGTCTTATTAGGCATCACAGATCCTGAATTGCATATTTTGGATGAGTTTGAGGATGTTGAGTATCAGAGGAGCACTGTTGAGGTCTCTTTGATGGATAGTTCTGAGAACTTACGAGTTCATACTTATGTCTGGTGTGACAAAAATGACCCAAACTTGTATGGAGAATGGGATTTTGAGGTATGGAAACAAACGCATATGGAAGACTTTATGAAGATGACTTCTGGTTTCATGGAAGAGCTGGAGCAACCTGAATCAAAGCCTAGAGTTGCAACATACGAATCCTTTTATCAGAATGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

171

Amino Acids

19.5

Weight (kDa)

4.82

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 16 - 127 9.7e-26 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 54
AciI CCGC 2 cut(s) 71, 114
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 46
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 2 cut(s) 35, 70
AflIII ACRYGT 2 cut(s) 45, 160
AgeI ACCGGT 1 cut(s) 181
AgsI TTSAA 1 cut(s) 97
AjiI CACGTC 1 cut(s) 161
AluBI AGCT 1 cut(s) 448
AluI AGCT 1 cut(s) 448
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 1 cut(s) 37
ApeKI GCWGC 1 cut(s) 60
AsiGI ACCGGT 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 1 cut(s) 72
BccI CCATC 4 cut(s) 10, 61, 295, 500
BceAI ACGGC 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 2 cut(s) 471, 511
BisI GCNGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 161
BmsI GCATC 2 cut(s) 159, 231
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 298
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 70
Bse118I RCCGGY 2 cut(s) 181, 203
BseGI GGATG 2 cut(s) 256, 268
BseLI CCNNNNNNNGG 2 cut(s) 35, 70
BseMII CTCAG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 72
BshFI GGCC 1 cut(s) 39
BshTI ACCGGT 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 284
BsiSI CCGG 3 cut(s) 170, 182, 204
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 35, 70
BsmAI GTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 39
Bso31I GGTCTC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 2 cut(s) 71, 114
BspANI GGCC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 224
BspQI GCTCTTC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 298
BsrFI RCCGGY 2 cut(s) 181, 203
BssAI RCCGGY 2 cut(s) 181, 203
BssMI GATC 1 cut(s) 229
BssNI GRCGYC 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 2 cut(s) 256, 268
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 1 cut(s) 229
BstPAI GACNNNNGTC 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 39
BtgZI GCGATG 1 cut(s) 29
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 2 cut(s) 256, 268
BtsIMutI CAGTG 2 cut(s) 15, 282
CaiI CAGNNNCTG 1 cut(s) 233
Cfr10I RCCGGY 2 cut(s) 181, 203
CseI GACGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 145
CspAI ACCGGT 1 cut(s) 181
CviAII CATG 1 cut(s) 439
CviJI RGCY 6 cut(s) 39, 63, 135, 448, 469, 510
CviKI_1 RGCY 6 cut(s) 39, 63, 135, 448, 469, 510
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 312
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
DraIII CACNNNGTG 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 86
Eco31I GGTCTC 1 cut(s) 298
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
FaeI CATG 1 cut(s) 442
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 438
FauNDI CATATG 1 cut(s) 405
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 2 cut(s) 263, 275
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 2 cut(s) 471, 511
GluI GCNGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 1 cut(s) 39
HapII CCGG 3 cut(s) 170, 182, 204
HgaI GACGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 1 cut(s) 442
HinfI GANTC 6 cut(s) 87, 97, 102, 175, 461, 488
HpaII CCGG 3 cut(s) 170, 182, 204
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 4 cut(s) 30, 276, 313, 501
Hpy188III TCNNGA 2 cut(s) 94, 233
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 3 cut(s) 45, 78, 160
HpyCH4V TGCA 2 cut(s) 241, 479
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 3 cut(s) 45, 78, 160
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 1 cut(s) 442
Kzo9I GATC 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 279, 451
LpnPI CCDG 9 cut(s) 77, 183, 195, 217, 246, 325, 417, 434, 471
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 2 cut(s) 159, 231
MaeI CTAG 2 cut(s) 471, 511
MaeII ACGT 3 cut(s) 45, 78, 160
MaeIII GTNAC 2 cut(s) 199, 344
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 3 cut(s) 422, 433, 455
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 1 cut(s) 236
MlyI GAGTC 2 cut(s) 96, 111
MnlI CCTC 7 cut(s) 67, 101, 117, 253, 270, 283, 379
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 3 cut(s) 170, 182, 204
NdeI CATATG 1 cut(s) 405
NdeII GATC 1 cut(s) 229
NlaIII CATG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 4 cut(s) 97, 175, 461, 488
PinAI ACCGGT 1 cut(s) 181
PkrI GCNGC 1 cut(s) 62
PleI GAGTC 2 cut(s) 95, 110
PpsI GAGTC 2 cut(s) 95, 110
PshAI GACNNNNGTC 1 cut(s) 81
PspEI GGTNACC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 438
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 1 cut(s) 229
SchI GAGTC 2 cut(s) 96, 111
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 2 cut(s) 159, 231
SmiMI CAYNNNNRTG 1 cut(s) 333
Sse9I AATT 1 cut(s) 236
SsiI CCGC 2 cut(s) 71, 114
SspMI CTAG 2 cut(s) 471, 511
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 3 cut(s) 48, 81, 163
TaqI TCGA 2 cut(s) 85, 100
TasI AATT 1 cut(s) 236
TfiI GAWTC 4 cut(s) 97, 175, 461, 488
TscAI CASTG 2 cut(s) 15, 289
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 317, 427, 434
TspRI CASTG 2 cut(s) 15, 289
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 471, 511
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.