MD15G1103200.v1.1

AIG2-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010238
Physical Location & Seq
Reverse (-)
7289214 .. 7290687
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1103200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGAGTGGTGGTGCGATGAGTTCAGTGGTGGCGAACGTTGGTGGTCAGTCGCAGAGCCTCCACAAGGTGTTCGTGTACGGCAGCCTCATGGCGGAGGATGTCTGCCGTGTCCTCTTAAATCGCGTCCCTCAATCCTCCCCTGCCCTCCTCAATGGCTATCATAGGTACAGCATCAAAGGAAGAGTTTATCCGGCGATTGTACCCGTCGAGAATAAAACAGTTGCCGGCAAGGTGCTGTTGGGCATCACAGATCCTGAATTACATATTTTAGATGAATTTGAGGATGTTGAGTATGAAAGAAGCACTGTTGAGGTCTCTTTGATGGATACTTCTGAGAGCTTGCTTGTTCAAGCATACGTTTGGAGCAACAAAAATGATCCGAACTTGTACGGAGACTGGGATTTCGAGGAATGGAAACAACTACACATGAAAGATTTCGTCAAGATGACTTCGGGTTTTATGGAAAAGCTGGAGCTGCCCGAAGCAAAGCCAAGGGTTGCAACATACGAGTCGTTCTTTCAGCAGGATCGCGAGAATCCGCCCGCGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

184

Amino Acids

20.64

Weight (kDa)

4.92

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 23 - 134 3.1e-25 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 123, 531, 545
AciI CCGC 3 cut(s) 92, 539, 543
AclI AACGTT 1 cut(s) 36
AclWI GGATC 3 cut(s) 245, 371, 534
AcsI RAATTY 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 4 cut(s) 77, 167, 201, 389
AfiI CCNNNNNNNGG 2 cut(s) 64, 91
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 3 cut(s) 339, 469, 475
AluI AGCT 3 cut(s) 339, 469, 475
Alw26I GTCTC 2 cut(s) 319, 387
AlwI GGATC 3 cut(s) 245, 371, 534
AlwNI CAGNNNCTG 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 81, 475
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp700I GAANNNNTTC 1 cut(s) 434
BbvI GCAGC 2 cut(s) 93, 462
BccI CCATC 1 cut(s) 316
BceAI ACGGC 2 cut(s) 90, 94
BciVI GTATCC 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 319, 387
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 2 cut(s) 82, 476
BlsI GCNGC 2 cut(s) 83, 477
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 180, 252
BmuI ACTGGG 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 91
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 2 cut(s) 103, 289
BseLI CCNNNNNNNGG 2 cut(s) 64, 91
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 2 cut(s) 93, 462
Bsh1236I CGCG 3 cut(s) 123, 531, 545
BsiSI CCGG 2 cut(s) 191, 225
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 64, 91
BsmAI GTCTC 2 cut(s) 319, 387
BsmFI GGGAC 1 cut(s) 110
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 3 cut(s) 250, 376, 526
Bsp68I TCGCGA 1 cut(s) 531
BspACI CCGC 3 cut(s) 92, 539, 543
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 3 cut(s) 123, 531, 545
BspPI GGATC 3 cut(s) 245, 371, 534
BspTNI GGTCTC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 1 cut(s) 401
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 3 cut(s) 250, 376, 526
BssT1I CCWWGG 1 cut(s) 491
Bst4CI ACNGT 2 cut(s) 220, 307
Bst6I CTCTTC 1 cut(s) 175
BstC8I GCNNGC 3 cut(s) 226, 341, 543
BstDEI CTNAG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 103, 289
BstFNI CGCG 3 cut(s) 123, 531, 545
BstKTI GATC 3 cut(s) 253, 379, 529
BstMAI GTCTC 2 cut(s) 319, 387
BstMBI GATC 3 cut(s) 250, 376, 526
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstUI CGCG 3 cut(s) 123, 531, 545
BstV1I GCAGC 2 cut(s) 93, 462
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuI GTATCC 1 cut(s) 319
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 2 cut(s) 103, 289
BtsIMutI CAGTG 2 cut(s) 30, 303
BtuMI TCGCGA 1 cut(s) 531
Cac8I GCNNGC 3 cut(s) 226, 341, 543
CaiI CAGNNNCTG 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 224
CseI GACGC 2 cut(s) 112, 534
Csp6I GTAC 4 cut(s) 76, 166, 200, 388
CviAII CATG 2 cut(s) 88, 427
CviJI RGCY 7 cut(s) 57, 84, 156, 339, 469, 475, 490
CviKI_1 RGCY 7 cut(s) 57, 84, 156, 339, 469, 475, 490
CviQI GTAC 4 cut(s) 76, 166, 200, 388
DdeI CTNAG 1 cut(s) 333
DpnI GATC 3 cut(s) 252, 378, 528
DpnII GATC 3 cut(s) 250, 376, 526
DraIII CACNNNGTG 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
EciI GGCGGA 2 cut(s) 107, 528
Eco130I CCWWGG 1 cut(s) 491
Eco31I GGTCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 2 cut(s) 91, 430
FaiI YATR 9 cut(s) 89, 162, 264, 294, 355, 428, 461, 505, 550
FaqI GGGAC 1 cut(s) 110
FatI CATG 2 cut(s) 87, 426
FauI CCCGC 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 82, 476
FokI GGATG 2 cut(s) 110, 296
Fsp4HI GCNGC 2 cut(s) 82, 476
GluI GCNGC 2 cut(s) 82, 476
GsuI CTGGAG 1 cut(s) 491
HapII CCGG 2 cut(s) 191, 225
HgaI GACGC 2 cut(s) 112, 534
Hin1II CATG 2 cut(s) 91, 430
HinfI GANTC 2 cut(s) 509, 535
HpaII CCGG 2 cut(s) 191, 225
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 334, 381
Hpy188III TCNNGA 4 cut(s) 208, 254, 442, 530
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 220, 307
HpyCH4IV ACGT 2 cut(s) 36, 357
HpyCH4V TGCA 1 cut(s) 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 36, 357
Hsp92II CATG 2 cut(s) 91, 430
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 250, 376, 526
LmnI GCTCC 2 cut(s) 363, 472
LpnPI CCDG 7 cut(s) 153, 204, 238, 267, 382, 455, 509
Lsp1109I GCAGC 2 cut(s) 93, 462
LweI GCATC 2 cut(s) 180, 252
MaeII ACGT 2 cut(s) 36, 357
MalI GATC 3 cut(s) 252, 378, 528
MboI GATC 3 cut(s) 250, 376, 526
MboII GAAGA 1 cut(s) 192
MflI RGATCY 1 cut(s) 250
MluCI AATT 2 cut(s) 257, 275
MlyI GAGTC 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 434
MseI TTAA 1 cut(s) 116
MspI CCGG 2 cut(s) 191, 225
MvnI CGCG 3 cut(s) 123, 531, 545
MwoI GCNNNNNNNGC 1 cut(s) 475
NaeI GCCGGC 1 cut(s) 226
NdeII GATC 3 cut(s) 250, 376, 526
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 2 cut(s) 91, 430
NruI TCGCGA 1 cut(s) 531
PdiI GCCGGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 535
PkrI GCNGC 2 cut(s) 83, 477
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp1406I AACGTT 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 1 cut(s) 250
RruI TCGCGA 1 cut(s) 531
RsaI GTAC 4 cut(s) 77, 167, 201, 389
RsaNI GTAC 4 cut(s) 76, 166, 200, 388
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 2 cut(s) 82, 476
Sau3AI GATC 3 cut(s) 250, 376, 526
SchI GAGTC 1 cut(s) 518
SetI ASST 9 cut(s) 39, 69, 167, 234, 315, 341, 360, 471, 477
SfaNI GCATC 2 cut(s) 180, 252
Sse9I AATT 2 cut(s) 257, 275
SsiI CCGC 3 cut(s) 92, 539, 543
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 2 cut(s) 220, 307
TaiI ACGT 2 cut(s) 39, 360
TaqI TCGA 2 cut(s) 207, 405
TasI AATT 2 cut(s) 257, 275
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 2 cut(s) 30, 310
TseI GCWGC 2 cut(s) 81, 475
TspDTI ATGAA 3 cut(s) 288, 309, 443
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 30, 310
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 1 cut(s) 275
XmnI GAANNNNTTC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.