AT4G31340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007104
Physical Location & Seq
Forward (+)
15205437 .. 15209604
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31340.2

Sequence Viewer

Length: 1314 bp
ATGGCGGCTGCGAAGCTCGTCGCTTTGTTACTTCTTGCCTTGCTTTTCACCACCAGCGTCTTCGCCGATGCTGATGAGCCGGAGGTTTCTGAGGCTGCCGGATCCGATGGCTCTTCCAAGATCCACCTGGATCAGCTTAATGCCAAGATCCGTGCCCTAGAATCTCAAATCGATGAGAAAACCCGGGAAGTTCAGGGCAAAGACGAGGTAGTAGCAGAGAAGGAGAAACTACTCAAAGAAAGGGAAGACAAGATTGCTTCCTTGCAGACTGAAGTCTCATCACTACAGAAAAAGGGATCATCAGATTCTGCAAAACAGTTGGGAAAGGCTCAAGCACGAGCTGATGAATTAGAGAAGCAGGTTGAGGTTCTCAAGAACTTCTTGGAGCAAAAAAACAAGGAGAAAGATTCCACTGAAGCTCGGACTAATGAAGCTGAGAAAAAACTAAGGGAACTTAACTCGAGCCTTGACAAACTTCAGAAGACAAATGAAGAGCAGAAGAACAAAATAGGAAAACTTGAACGTGCTATTAAGATAGCTGAGGAAGAAATGTTGAGGACAAAGCTCGAAGCAACCACAAAGGCGAAGGAACTGCTAGAGGCTCATGGTTCGTGGCTTCCCCCTTGGCTTGCTGTTCACTGGTTTAAATTTCAGACTTATACCGAGACACATTGGGAGGCCCATGGGAAACCTGCTGTGGAGACAGTAATTCTAAAGGTGACGGAAGCAAAAGCTCAAGCTGAAAAGTGGGCTGAACCACATGTCGAAAACGTTAAAACTAAATATATTCCAGCCATCAAGGAAACCGTTACTATACATGTTGAGCCACACTTCCGAACACTATCCATCAAAGCTAAAGAGGCTTACCATTCATCAAAGAGTGCTGTTTCCCCTCACATTGTCACGGTTCAAGAATTTGTAGACCCCTATTATCAGGAGGCCAAAAAGTTCAGCAAGCCATATGTCGACCAAGTGGCCACGACCACGAAACCACATGTTGATAAAATGAAGGTTGCCATGAAACCTTACACAACCAAGGTGATCATCGTCTATACAGAGTTCCTTGAATCCGCCACAACTTATCACAATCAGGTCCAAGCCCATGTTGAACGAAAACTGAAAAGCCATGAGCTGACGGAGCCTTTTGCTACCAATGAGTTTGTTTGGTTTGCGGCCTCTGCATTGTTGGTATTTCCCATCTTCGTTGCTTACAGAGTGCTCTCTTCTCTCTTCTGCACAAAGACAAAGAAACCTGTTAAACACCCTCATCACCATGGTCGTCGTAAGGCCAAAAGGGCTCACACTGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

437

Amino Acids

49.65

Weight (kDa)

9.03

Isoelectric Point (pI)

25.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 349, 700
AccI GTMKAC 2 cut(s) 921, 966
AciI CCGC 3 cut(s) 5, 1071, 1172
AclI AACGTT 1 cut(s) 771
AclWI GGATC 6 cut(s) 96, 109, 115, 138, 142, 304
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 2 cut(s) 647, 914
AcuI CTGAAG 3 cut(s) 291, 435, 461
AflIII ACRYGT 3 cut(s) 760, 817, 994
AgsI TTSAA 4 cut(s) 521, 911, 1067, 1109
AjnI CCWGG 1 cut(s) 126
Alw21I GWGCWC 1 cut(s) 1221
Alw26I GTCTC 3 cut(s) 280, 659, 695
AlwI GGATC 6 cut(s) 96, 109, 115, 138, 142, 304
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 2 cut(s) 183, 460
AoxI GGCC 5 cut(s) 678, 939, 975, 1173, 1287
ApeKI GCWGC 2 cut(s) 8, 95
ApoI RAATTY 2 cut(s) 647, 914
AspS9I GGNCC 2 cut(s) 679, 1093
AsuC2I CCSGG 2 cut(s) 184, 185
AsuHPI GGTGA 4 cut(s) 40, 730, 1051, 1262
AvaI CYCGRG 2 cut(s) 183, 460
AvaII GGWCC 1 cut(s) 1093
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 977
BamHI GGATCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 1300
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 3 cut(s) 52, 252, 488
Bbv12I GWGCWC 1 cut(s) 1221
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 1 cut(s) 82
BccI CCATC 4 cut(s) 101, 803, 854, 1205
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 1041
BcnI CCSGG 2 cut(s) 184, 185
BcoDI GTCTC 3 cut(s) 280, 659, 695
BfaI CTAG 2 cut(s) 158, 596
BfmI CTRYAG 1 cut(s) 284
BfuAI ACCTGC 2 cut(s) 349, 700
BglI GCCNNNNNGGC 1 cut(s) 1295
BisI GCNGC 4 cut(s) 6, 9, 96, 1173
BlsI GCNGC 4 cut(s) 7, 10, 97, 1174
Bme1390I CCNGG 3 cut(s) 128, 184, 185
Bme18I GGWCC 1 cut(s) 1093
BmeT110I CYCGRG 2 cut(s) 183, 460
BmgT120I GGNCC 2 cut(s) 679, 1093
BmiI GGNNCC 2 cut(s) 103, 1140
BmrFI CCNGG 3 cut(s) 128, 184, 185
BmsI GCATC 1 cut(s) 58
BoxI GACNNNNGTC 1 cut(s) 272
BpiI GAAGAC 3 cut(s) 52, 252, 488
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 3 cut(s) 315, 356, 720
BpuMI CCSGG 2 cut(s) 184, 185
Bsa29I ATCGAT 1 cut(s) 171
BsaJI CCNNGG 5 cut(s) 183, 623, 682, 1035, 1273
Bse1I ACTGG 1 cut(s) 644
BseBI CCWGG 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 5 cut(s) 183, 623, 682, 1035, 1273
BseMII CTCAG 3 cut(s) 81, 426, 531
BseNI ACTGG 1 cut(s) 644
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 1219
BshFI GGCC 5 cut(s) 680, 941, 977, 1175, 1289
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 1221
BsiHKCI CYCGRG 2 cut(s) 183, 460
BsiSI CCGG 3 cut(s) 80, 99, 184
BsmAI GTCTC 3 cut(s) 280, 659, 695
BsnI GGCC 5 cut(s) 680, 941, 977, 1175, 1289
BsoBI CYCGRG 2 cut(s) 183, 460
Bsp1286I GDGCHC 3 cut(s) 157, 1221, 1300
Bsp143I GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
Bsp19I CCATGG 2 cut(s) 682, 1273
BspACI CCGC 3 cut(s) 5, 1071, 1172
BspANI GGCC 5 cut(s) 680, 941, 977, 1175, 1289
BspCNI CTCAG 3 cut(s) 82, 427, 532
BspDI ATCGAT 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 103, 1140
BspMI ACCTGC 2 cut(s) 349, 700
BspPI GGATC 6 cut(s) 96, 109, 115, 138, 142, 304
BspQI GCTCTTC 2 cut(s) 118, 486
BsrI ACTGG 1 cut(s) 644
BssECI CCNNGG 5 cut(s) 183, 623, 682, 1035, 1273
BssMI GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
BssSI CACGAG 1 cut(s) 336
BssT1I CCWWGG 4 cut(s) 623, 682, 1035, 1273
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 4 cut(s) 318, 706, 808, 907
Bst6I CTCTTC 4 cut(s) 118, 486, 1228, 1235
BstC8I GCNNGC 2 cut(s) 630, 956
BstDEI CTNAG 4 cut(s) 90, 435, 446, 540
BstDSI CCRYGG 2 cut(s) 682, 1273
BstKTI GATC 6 cut(s) 104, 123, 133, 150, 299, 1044
BstMAI GTCTC 3 cut(s) 280, 659, 695
BstMBI GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
BstMWI GCNNNNNNNGC 4 cut(s) 860, 1138, 1178, 1295
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 3 cut(s) 764, 821, 998
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 126, 182, 183
BstSFI CTRYAG 1 cut(s) 284
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 82
BstV2I GAAGAC 3 cut(s) 52, 252, 488
BstX2I RGATCY 3 cut(s) 101, 120, 147
BstYI RGATCY 3 cut(s) 101, 120, 147
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 5 cut(s) 680, 941, 977, 1175, 1289
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 2 cut(s) 682, 1273
BtsIMutI CAGTG 3 cut(s) 411, 637, 1302
BveI ACCTGC 2 cut(s) 349, 700
Cac8I GCNNGC 2 cut(s) 630, 956
CaiI CAGNNNCTG 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 679, 1093
Cfr9I CCCGGG 1 cut(s) 183
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 1 cut(s) 46
CviAII CATG 9 cut(s) 605, 683, 761, 818, 995, 1018, 1103, 1127, 1274
DdeI CTNAG 4 cut(s) 90, 435, 446, 540
DpnI GATC 6 cut(s) 103, 122, 132, 149, 298, 1043
DpnII GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 4 cut(s) 118, 486, 1228, 1235
EarI CTCTTC 4 cut(s) 118, 486, 1228, 1235
EciI GGCGGA 1 cut(s) 1060
Eco130I CCWWGG 4 cut(s) 623, 682, 1035, 1273
Eco24I GRGCYC 1 cut(s) 1300
Eco47I GGWCC 1 cut(s) 1093
Eco57I CTGAAG 3 cut(s) 291, 435, 461
Eco88I CYCGRG 2 cut(s) 183, 460
EcoRII CCWGG 1 cut(s) 126
EcoT14I CCWWGG 4 cut(s) 623, 682, 1035, 1273
EcoT38I GRGCYC 1 cut(s) 1300
ErhI CCWWGG 4 cut(s) 623, 682, 1035, 1273
FaeI CATG 9 cut(s) 608, 686, 764, 821, 998, 1021, 1106, 1130, 1277
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 9 cut(s) 604, 682, 760, 817, 994, 1017, 1102, 1126, 1273
FauNDI CATATG 1 cut(s) 961
FbaI TGATCA 1 cut(s) 1041
FblI GTMKAC 2 cut(s) 921, 966
Fnu4HI GCNGC 4 cut(s) 6, 9, 96, 1173
FriOI GRGCYC 1 cut(s) 1300
Fsp4HI GCNGC 4 cut(s) 6, 9, 96, 1173
FspBI CTAG 2 cut(s) 158, 596
GluI GCNGC 4 cut(s) 6, 9, 96, 1173
HaeIII GGCC 5 cut(s) 680, 941, 977, 1175, 1289
HapII CCGG 3 cut(s) 80, 99, 184
HgaI GACGC 1 cut(s) 46
Hin1II CATG 9 cut(s) 608, 686, 764, 821, 998, 1021, 1106, 1130, 1277
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 4 cut(s) 161, 305, 407, 1067
HpaII CCGG 3 cut(s) 80, 99, 184
HphI GGTGA 4 cut(s) 40, 730, 1051, 1262
Hpy166II GTNNAC 3 cut(s) 637, 922, 967
Hpy188I TCNGA 7 cut(s) 91, 106, 304, 423, 480, 654, 836
Hpy188III TCNNGA 3 cut(s) 373, 911, 935
Hpy8I GTNNAC 3 cut(s) 637, 922, 967
Hpy99I CGWCG 2 cut(s) 23, 1284
HpyAV CCTTC 3 cut(s) 214, 580, 1003
HpyCH4III ACNGT 4 cut(s) 318, 706, 808, 907
HpyCH4IV ACGT 2 cut(s) 523, 771
HpyCH4V TGCA 4 cut(s) 265, 311, 1181, 1236
HpyF10VI GCNNNNNNNGC 4 cut(s) 860, 1138, 1178, 1295
HpyF3I CTNAG 4 cut(s) 90, 435, 446, 540
HpySE526I ACGT 2 cut(s) 523, 771
Hsp92II CATG 9 cut(s) 608, 686, 764, 821, 998, 1021, 1106, 1130, 1277
Ksp22I TGATCA 1 cut(s) 1041
Kzo9I GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
LguI GCTCTTC 2 cut(s) 118, 486
LmnI GCTCC 2 cut(s) 385, 1138
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 58
MaeI CTAG 2 cut(s) 158, 596
MaeII ACGT 2 cut(s) 523, 771
MaeIII GTNAC 4 cut(s) 27, 718, 808, 901
MalI GATC 6 cut(s) 103, 122, 132, 149, 298, 1043
MboI GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
MflI RGATCY 3 cut(s) 101, 120, 147
MhlI GDGCHC 3 cut(s) 157, 1221, 1300
MlsI TGGCCA 1 cut(s) 977
MluCI AATT 4 cut(s) 347, 647, 708, 914
MluNI TGGCCA 1 cut(s) 977
Mox20I TGGCCA 1 cut(s) 977
MscI TGGCCA 1 cut(s) 977
MseI TTAA 6 cut(s) 138, 456, 531, 645, 774, 1257
MslI CAYNNNNRTG 1 cut(s) 1272
Msp20I TGGCCA 1 cut(s) 977
MspI CCGG 3 cut(s) 80, 99, 184
MspR9I CCNGG 3 cut(s) 128, 184, 185
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 860, 1138, 1178, 1295
NciI CCSGG 2 cut(s) 184, 185
NcoI CCATGG 2 cut(s) 682, 1273
NdeI CATATG 1 cut(s) 961
NdeII GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
NlaIII CATG 9 cut(s) 608, 686, 764, 821, 998, 1021, 1106, 1130, 1277
NlaIV GGNNCC 2 cut(s) 103, 1140
NmuCI GTSAC 2 cut(s) 718, 901
NspI RCATGY 3 cut(s) 764, 821, 998
PaeR7I CTCGAG 1 cut(s) 460
PciI ACATGT 3 cut(s) 760, 817, 994
PciSI GCTCTTC 2 cut(s) 118, 486
PfeI GAWTC 4 cut(s) 161, 305, 407, 1067
PkrI GCNGC 4 cut(s) 7, 10, 97, 1174
PscI ACATGT 3 cut(s) 760, 817, 994
PshAI GACNNNNGTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 103, 1140
PspPI GGNCC 2 cut(s) 679, 1093
PspXI VCTCGAGB 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 3 cut(s) 101, 120, 147
RseI CAYNNNNRTG 1 cut(s) 1272
SalI GTCGAC 1 cut(s) 965
SapI GCTCTTC 2 cut(s) 118, 486
SaqAI TTAA 6 cut(s) 138, 456, 531, 645, 774, 1257
SatI GCNGC 4 cut(s) 6, 9, 96, 1173
Sau3AI GATC 6 cut(s) 101, 120, 130, 147, 296, 1041
Sau96I GGNCC 2 cut(s) 679, 1093
ScrFI CCNGG 3 cut(s) 128, 184, 185
SduI GDGCHC 3 cut(s) 157, 1221, 1300
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 1 cut(s) 284
Sfr274I CTCGAG 1 cut(s) 460
SinI GGWCC 1 cut(s) 1093
SlaI CTCGAG 1 cut(s) 460
SmaI CCCGGG 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 1272
SmlI CTYRAG 4 cut(s) 330, 371, 460, 735
SmoI CTYRAG 4 cut(s) 330, 371, 460, 735
Sse9I AATT 4 cut(s) 347, 647, 708, 914
SsiI CCGC 3 cut(s) 5, 1071, 1172
SspMI CTAG 2 cut(s) 158, 596
StyD4I CCNGG 3 cut(s) 126, 182, 183
StyI CCWWGG 4 cut(s) 623, 682, 1035, 1273
TaaI ACNGT 4 cut(s) 318, 706, 808, 907
TaiI ACGT 2 cut(s) 526, 774
TaqI TCGA 5 cut(s) 171, 461, 567, 765, 966
TasI AATT 4 cut(s) 347, 647, 708, 914
TauI GCSGC 2 cut(s) 8, 1175
TfiI GAWTC 4 cut(s) 161, 305, 407, 1067
Tru1I TTAA 6 cut(s) 138, 456, 531, 645, 774, 1257
Tru9I TTAA 6 cut(s) 138, 456, 531, 645, 774, 1257
TscAI CASTG 3 cut(s) 418, 644, 1309
TseFI GTSAC 2 cut(s) 718, 901
TseI GCWGC 2 cut(s) 8, 95
Tsp45I GTSAC 2 cut(s) 718, 901
TspDTI ATGAA 6 cut(s) 360, 444, 504, 861, 1022, 1034
TspGWI ACGGA 3 cut(s) 140, 737, 1151
TspMI CCCGGG 1 cut(s) 183
TspRI CASTG 3 cut(s) 418, 644, 1309
VpaK11BI GGWCC 1 cut(s) 1093
XapI RAATTY 2 cut(s) 647, 914
XceI RCATGY 3 cut(s) 764, 821, 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 183
XmiI GTMKAC 2 cut(s) 921, 966
XspI CTAG 2 cut(s) 158, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.